• Korean Journal of Fisheries and Aquatic Sciences
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• v.50 no.5
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• pp.506-519
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• 2017

This study evaluated the protein recovery and functional properties and biological activity of boiled and steamed process water (BPW and SPW, respectively) generated from the preparation of concentrated roe of bastard halibut (BH; Paralichthys olivaceus), skipjack tuna (ST; Katsuwonus pelamis), and yellowfin tuna (YT; Thunnus albacares) using the cook-dry process. The protein loss from the water extracts (EXT) of 100 g of roe protein was 15.05-19.71% and was significantly (P<0.05) higher than that of BPW (5.47-10.34%) and SPW (3.88-8.18%). The foam capacity of BPW (166-203%) and SPW (15-194%) was better than that of EXT. The emulsifying activity index of the original samples was lower than those ($15.40-107.86m^2/g$) of diluted protein samples. The 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity and the reducing power of BPW and SPW were stronger than those of EXT. The 2,2'-azino-bis-3-ethylbenzothiazoline-6-sulfonic acid ($ABTS^+$) radical scavenging activity of EXT (0.028-0.045mg/mL) was significantly higher those of BPW and SPW. The angiotensin I-converting enzyme (ACE) inhibitory activity of SPW was the highest for BH (1.04 mg/mL), followed by YT and ST. The predominant amino acids in SPW were Glu, Ala, Leu, and His. These results demonstrate that processing water containing diluted organic components, including protein, can be consumed directly by humans as a functional reinforcing material after appropriate concentration processes.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.46 no.2
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• pp.119-128
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• 2013

A protease inhibitor was fractionated from fish eggs using methods based on protein solubility. Fractionation efficiency was evaluated with regard to percent recovery and total inhibitory activity (U). The fractionation of protease inhibitor (PI) from egg extracts of skipjack tuna (ST, Katsuwonus pelamis), yellowfin tuna (YT, Thunnus albacores), and Alaska pollock (AP, Theragra chalcogramma) was performed by precipitation with cold acetone or ammonium sulfate (AS). Fractions exhibiting the strongest inhibitory activity contained 20-40% (v/v) cold acetone or 40-60% saturated AS fractions. AS fractionation was more effective in isolating PI than was precipitation with acetone. The total inhibitory activity and percent recovery of fraction obtained with AS 40-60% toward trypsin and $N{\alpha}$-benzoyl-L-arginine-p-nitroanilide (BAPNA) were 4,976 U and 24.2% for ST, 3,331 U and 38.1% for YT, and 4,750 U and 43.8% for AP, respectively. In comparisons against six commercial proteases, 40-80% AS fractions, made by combining the 40-60% and 60-80% AS fractions from fish egg extract, exhibited the strongest inhibition of trypsin when using a casein substrate. These results suggest that fish eggs act as serine protease inhibitors and may be useful for protease inhibition in foodstuffs.

• Food Science and Preservation
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• v.16 no.2
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• pp.286-291
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• 2009

Microorganisms in seafood cooking drips were counted and identified. Total viable cell counts were 6.40 and 3.10 log CFU/g in cooking drips of Hizikia fusiformis and Thunnus thynnus, respectively. However, microbial populations fell with increased irradiation doses. In H. fusiformis cooking drips, a 5-log reduction in total aerobic bacteria was obtained by irradiation with 5 kGy. In T. thynnus cooking drips, however, contaminating microorganisms were more resistant to gamma irradiation and only a 1-log reduction was seen. DNA sequence analysis showed that the principal contaminating microorganisms in H. fusiformis and T. thynnus cooking drips were Lactobacillus and Bacillus species, respectively. Therefore, the high irradiation resistance of T. thynnus cooking drips microbes may result from spore formation by Bacillus species.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.29 no.2
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• pp.197-207
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• 1996

The proportion of log-associated school catches by Korean tuna purse seiners in the western Pacific has shown a declining trend until recent years. During the period $1990\~1995$, log-associated school catches contributed $34.6\%$ to the total Korean tuna purse seine catch, representing quite a low level compared to the early phase of the purse seine fishery. Species compositions of both log-associated and free-school catches showed that skipjack, Katswonus pelamis, was dominant species and yellowfin, Thunnus albacares, followed, with the small amount of bigeye tunas, T. obesus, Yellowfin proportion was higher in free-school catches than in log-associated school catches. Log-associated school catches monitored during the scientific observation period were made of $60\%$ skipjack, $38\%$ yellowfin, and $2\%$ bigeye tunas, indicating the low skipjack and high yellowfin proportion compared with historical fisheries data based on logbooks. A total of 11 by-catch species were identified, of which sharks occurred together with tunas in all sets and yellowtail kingfish was the most abundant by-catch species. From the length distribution it was found that small yellowfin less than 70 cm mainly distributed around floating objects.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.10 no.1
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• pp.37-47
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• 1977

Korea started the Atlantic tuna fishery from 1964 by means of longline, then added bait-boats in 1972. Both fisheries have given the top priority to catch yellowfin tuna(Thunnus albacares). The paper reviews available catch, effort and biological data, estimates some population parameters in order to understand the status of the Atlantic yellowfin tuna as a whole. The main findings are summarized as follows: 1. The total of 476 million hook-equivalent fishing effort was thrown to catch yellowfin tuna in 1974, among which one thired was shared by longliners. 2. The dominant age group becomes younger in both surface and longline fisheries. 3. The recent mortality coefficients were calculated as 1.5 for total mortality and 0.7 for fishing mortality. 4. The weight at recruitment was 2.7 kg in 1973 which was smaller than the regulation size(3.2kg) proposed by ICCAT. 5. The maximum sustained yield was calculated to 95-145 thousand metric tons, which was the level of recent catch. Therefore, it is apparent that the present yellowfin tuna fishery should continue to receive close attention.

• Korean Journal of Ichthyology
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• v.20 no.2
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• pp.105-111
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• 2008

A pelagic tuna longline research cruise in the eastern and central Pacific Ocean from September to October of 2006 was conducted to compare catch rates with the use of different hook type and bait combinations. Traditional tuna hooks (J 4) and three circle hook types (C15, C16, C18), along with five bait types (chub mackerel (CM), jack mackerel (JM), milkfish (MF), sardine (SD), and squid (SQ)) and hook number as a proxy for hook depth were evaluated for their effect on bigeye tuna catch rates (fish per 1,000 hooks) using Generalized Linear Models (GLMs). Results from 28 sets indicated significant differences in bigeye catch rates between individual longline sets and hook number. The GLM explained 33% of the deviance in bigeye catch rates with these two factors. An alternative model formulation included bait type which had a small effect (explaining 2.7% of the deviance) on catch rates. Hook type had a negligible and non-significant effect in the GLMs. These results indicate that all of the hooks and baits tested are equally effective at catching bigeye tuna and that hook number (depth) was the paramount operational factor in explaining bigeye tuna catch rates.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.50 no.6
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• pp.694-706
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• 2017

This study evaluated the protein recovery, functional properties and biological activity of isolate processed water (IPW) generated in the preparation of protein isolates from fish roes (BH, bastard halibut Paralichthys olivaceus; ST, skipjack tuna Katsuwonus pelamis; YT, yellowfin tuna Thunnus albacares) by an isoelectric solubilization and precipitation process. The IPWs contained 2.7-5.4 mg/mL of protein, and the protein losses were 8-21% (P<0.05). The form capacity of IPW-3 for BH and ST, and IPW-4 for YT was 155, 194, and 164%, respectively. The emulsifying activity index ($27-43m^2/g$) of the YT-IPWs was the strongest, followed by ST ($7-29m^2/g$) and BH ($10-19m^2/g$). The 2,2-diphenyl-1-picrylhydrazyl scavenging activities of IPW-1 and -3 were higher than those of IPW-2 and -4. The 2,2'-azino-bis-3-ethylbenzothiazoline-6-sulfonic acid scavenging activity ($IC_{50}$, mg/mL) of IPW-2 and -4 was 0.03 mg/mL for BH, 0.04-0.08 mg/mL for ST, and 0.04-0.07 mg/mL for YT. BH IPW-3 had the strongest reducing power (0.41 mg/mL) and superoxide dismutase-like activity (1.68 mg/mL). The angiotensin-I converting enzyme inhibitory activity of IPW-3 was the highest for ST (1.52 mg/mL), followed by BH and YT. The common predominant amino acids in the IPWs were the essential amino acids Val, Leu, Lys, and Arg and the non-essential amino acids Ser, Glu, and Ala.

• KSBB Journal
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• v.23 no.6
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• pp.489-493
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• 2008

Although the seafood cooking drips were the byproducts from the fishery industry and being wasted, it had many nutrients including proteins. In this study, the effect of a gamma irradiation on the cooking drips from Hizikia fusiformis, Enteroctopus dofleni and Thunnus thynnus were investigated. The cooking drips were extracted with 70% ethanol solution, and the extracts were analysed for the protein concentration by three different methods of Lowry, BCA and Kjeldahl. The extracts were irradiated with different doses and the protein contents were compared with respect to the absorbed doses. Total content of the proteins was increased with increasing irradiation dose. The change of protein pattern in the irradiated cooking drips was also confirmed by SDS-PAGE analysis. These results shown that the proteins in cooking drips could be unfolded or aggregated by the irradiation. Therefore, gamma irradiation could be considered as an effective method for extracting useful proteins.

• Journal of Radiation Industry
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• v.2 no.1
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• pp.21-26
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• 2008

Although seafood cooking drips were the byproducts from the fishery industry it was known that the cooking drips had many nutrients and could be used as functional materials. Previously, the physiological properties of cooking drips were shown to be increased by a gamma irradiation. But, there was no report on the safe for the genotoxicity on the irradiation. In this study, the genotoxicity of the cooking drips from Hizikia fusiformis, Enteroctopus dofleni and Thunnus thynnus was evaluated by the Ames test (Salmonella typhimurium reversion assay) and the SOS chromotest. The results from all samples were negative in the bacterial reversion assay with S. typhimurium TA98, TA100. No mutagenicity was detected in the assay, both with and without metabolic activation. The SOS chromotest also indicated that the gamma-irradiated seafood cooking drips did not show any mutagenicity. Therefore, this study indicated that gamma irradiation could be used for the hygiene, functional properties and processibility of seafood cooking drips.

• Journal of the Korean Society of Fisheries and Ocean Technology
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• v.31 no.2
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• pp.153-165
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• 1995

The fluctuations of catches in set nets around Kyeongbuk Province, the eastern coast of Korea, were analyzed and investigated by on the values of CPUE(Catch Per Unit Effort per hauling), and composition of dominant species caught from 1985 to 1989. Annual CPUE values were fluctuated every year, but their trends were decreased year by year, When the values were evaluated by species, the trends of annual catches were shown decreasing in file fish(Auteridae), mackerel(Scomber japonicus), tuna(Thunnus Thynnus), rock fish(Sebastes schlegelid) and yellowtail(Seriola quinqueradiata), increasing in sardine(Sardinops melanosticta), jack mackerel(Trachurus japonicus), and herring(Clupea pallasi), and similar in squid(Todarodes pacificus) and cuttle fish(Sepiidae). The main fishing season evaluated by monthly CPUE was estimated from August to November with a little difference by regions : from August to November at Chukpyon and Kanggu, from September to November at Chuksan and Kampo, and August to December in Hupo. When the DPUE values were analyzed by species, the main fishing seasons were quite different by species. Mackerel, jack mackerel, tuna, yellowtail, and rock fish were caught mainly from September to October, file fish and squid from November to January, sardine from April to May, herring in May, and cuttle fish in April. Annual catches were shown highest level in file fish and revealed higher by sardine, jack mackerel, mackerel, squid, tuna, and yellowtail in order. But the highest catches among each species were different with seasons, and that from January to July was sardine, from November to December file fish. The main migrating seasons of file fish, mackerel, squid, tuna, and cuttle fish at Chukpyon were a little earlier than at other regions. Though the migrating seasons of jack mackerel and tuna were almost same in every regions, that of sardine were shown 3 month's difference according to regions. In the year when the warm currents were stronger than those of the normal year and their isotherms were formed from the north to south along the eastern coastal line, the annual fish catches in set net were show higher levels.