• 대한의용생체공학회:학술대회논문집
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• 대한의용생체공학회 1997년도 춘계학술대회
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• pp.51-54
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• 1997

Lumbrokinase, potent fibrinolytic enzyme purified from earthworm, was immobilized onto the total artificial heart valves using photoreaction. This valve were implanted into the lamb for three days. After experiments, thrombus was observed in the untreated valves whereas no thrombus was observed in the lumbrokinase immobilized valves. The fibrinolytic activity and proteolytic activity of the implanted valve was examined. The fibrinolytic activity of the valve was remained after the implantation. The lumbrokinase could be a suitable fibrinolytic agents in the vascular contacting devices to reduce the thrombus.

• 대한본초학회지
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• 제23권4호
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• pp.191-196
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• 2008

Objectives: This research was investigated to find out the effect of the anticoagulant Perillae folium extract. Methods: To examine an active effect of anticoagulation in Perillae folium extract, the study measured Prothrombin time(PT) and activated partial thromboplastin time(APTT) of human plasma in vitro and measured bleeding time and arterio-venous shunt model in rats in vivo. Results: Bleeding time of Perillae folium extract in vivo had a significant increase 1.6 times and thrombus weight of Perillae folium extract had a significant reduction of thrombus weight as 68%. Perillae folium extract had an effect of anticoagulation by operating on extrinsic pathway factor II, V, VII, X and intrinsic pathway factor VIII, IX, X, VI, VII in the coagulation system. Conclusions: Considering the above mentioned results, it is judged that a Perillae folium extract has a control effect of thrombus creation.

• 대한의용생체공학회:의공학회지
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• 제27권5호
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• pp.267-273
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• 2006

Numerical analysis was performed on the enzyme transport and the flow fields in order to predict the effectiveness of urokinase injection regimens in clot dissolution. The species and momentum transport equations were numerically solved for the case of uniform perfusion of enzyme into a fibrin clot for an arterial thrombus and a deep vein thrombus models. In order to predict the thrombus lysis efficiency of continuous and forced intermittent injections, enzyme perfusion and clot lysis were simulated for the different injection velocities. Intermittent injection showed faster clot lysis compared to continuous perfusion, and lysis efficiency was increased as injection velocity increased.

• 대한본초학회지
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• 제24권2호
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• pp.7-11
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• 2009

Objectives : This research was investigated anticoagulant effect of the Chaenomelis Fructus extract. Methods : To examine an active effect of anticoagulation in Chaenomelis Fructus extract, the study measured Prothrombin time(PT) and activated partial thromboplastin time (APTT) of human plasma in vitro and measured bleeding time and arterio-venous shunt model in rats in vivo. Results : Bleeding time of Chaenomelis Fructus extract in vivo had a significant increase as about 1.6 times and thrombus weight of Chaenomelis Fructus extract had a significant reduction of thrombus weight as 50%. Chaenomelis Fructus extract represented an effect of anticoagulation by operating on extrinsic pathway factor II, V, VII, X and intrinsic pathway factor VIII, IX, X, XI, XII in the coagulation system. Conclusions : Considering the above mentioned results, it is judged that a Chaenomelis Fructus extract has a control effect of thrombus creation.

• 대한의용생체공학회:의공학회지
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• 제22권5호
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• pp.439-448
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• 2001

이식형 양심실 보조 장치 (Biventricular Assist Device, BVAD)에서 판막이 닫힐 때 나는 소리의 특성과 판막의 물리적인 상태의 상관성을 고찰하였다. 본 연구에서 Bj rk Shiley Convexo Concave tilting disk 판막을 사용했으며, 모의 순환계와 양의 체내에서 동작하는 BVAD 에서 판막음을 측정하였다. 모의 순환계에서는 정상 판막. 기계적으로 손상된 판막. 모의 혈전이 형성된 판막의 3가지를 고려하였다. 양에 이식된 BVAD의 경우, 이식 후 1일부터 5일 동안 규칙적인 간격으로 판막음을 측정하였다. 측정된 신호의 스펙트럼 특성은 Multiple Signal Classification (MUSIC)을 이용하여 추정하였다. MUSIC의 최적 차수는 Bayesian Information Criterion (BIC)을 이용하여 계산하였다. 실험 결과, 판막의 기계적인 손상은 판막 폐쇄음의 주파수 스펙트럼 구조를 변화시키고 있으며, 혈전의 형성은 판막음 스펙트럼의 기본 구조는 유지하지만 피크 주파수와 에너지의 크기를 변화시키는 것으로 관찰되었다. 최대 에너지를 가지는 MSP (maximum spectral peak)는 정상 판막에서는 2 kHz에 위치하고 있으나 모의 혈전을 부탁한 판막에서는 3 kHz로 이동하였다. 손상된 판막은 7 kHz 부근에서 강한 피크 보이고 있다 실험 동물 내에서 판막에 혈전이 형성되어감에 따라 판막음은 저주파 성분 (〈 2kHz)이 상대적으로 크게 감소하였고, Ist 2nd. 3rd MSP 주파수는 약간씩 상승하였다. 또한 혈전이 형성되어 감에 따라 반복해서 측정된 판막음의 1st, 2nd. 3rd MSP 주파수의 변화 정도 및 BIC 차수는 감소하는 것으로 나타났다

• Journal of Yeungnam Medical Science
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• 제10권1호
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• pp.237-244
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• 1993

저자들은 승모판 협착증과 동반된 자유롭고 부유하는 좌심방내의 구형혈전은 1례를 치험하였기에 그 희귀성에 비추어 문헌 고찰과 함께 보고하는 바이다.

• Journal of Korean Neurosurgical Society
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• 제63권1호
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• pp.34-44
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• 2020

Objective : Despite widespread use of flow diverters (FDs) to treat aneurysms, the exact healing mechanism associated with FDs remains poorly understood. We aim to describe the healing process of aneurysms treated using FDs by demonstrating the histopathologic progression in a canine aneurysm model. Methods : Twenty-one side wall aneurysms were created in common carotid artery of eight dogs and treated with two different FDs. Angiographic follow-ups were done immediately after placement of the device, 4 weeks and 12 weeks. At last follow-up, the aneurysm and the device-implanted parent artery were harvested. Results : Histopathologic findings of aneurysms at 4 weeks follow-up showed intra-aneurysm thrombus formation in laminating fashion, and neointimal thickening at the mid-segment of aneurysm. However, there are inhomogenous findings in aneurysms treated with the same type of FD showing same angiographic outcomes. At 12 weeks, aneurysms of complete and near-complete occlusion revealed markedly shrunken aneurysm filled with organized connective tissues with thin neointima. Aneurysms of incomplete occlusion at 12 weeks showed small amount of organized thrombus around fringe neck and large empty space with thick neointmal formation. Neointimal thickness and diameter stenosis was not significantly different between the groups of FD specification and follow-up period. Conclusion : Intra-aneurysmal thrombus formation and organization seem to be an important factor for the complete occlusion of aneurysms treated using the FD. Neointimal formation could occur along the struts of the FD independently of intra-aneurysmal thrombus formation. However, neointimal formation could not solely lead to complete aneurysm healing.

• Journal of Chest Surgery
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• 제18권3호
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• pp.446-455
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• 1985

A total and consecutive 156 patients have undergone cardiac valve surgery including 13 closed mitral commissurotomy, 13 open mitral commissurotomy, one mitral annuloplasty, 75 mitral valve replacement, one aortic annuloplasty, 24 aortic valve replacement, 3 tricuspid valve replacement, 25 double valve replacement and one triple valve replacement. 155 prosthetic valves were replaced in a period between September 1976 and August 1985. There were 68 males and 88 females with age range from 8 to 69 yrs [mean 36.5 yr]. Out of replaced valves, 61 was tissue valve including 54 Carpentier-Edwards, and 4 was mechanical valves including 74 St. Jude Medical, and the position replaced was 101 valves for mitral, 46 for aortic and 8 for tricuspid. Single valve replacement in 102 cases, double valve replacement in 25 cases [17 for AVR+MVR, and 8 for MVR+TVR], and only one case was noted in the triple valve replacement. Early mortality within 30 days after operation was noted in 11 cases [7%]; 7 after MVR, 2 after DVR, and each one after open mitral commissurotomy and mitral annuloplasty. Cause of death was valve thrombus, cerebral air embolism, low output syndrome, uncontrollable arrhythmia, parapneumonic sepsis, acute cardiac tamponade and left atrial rupture. 7 late deaths were noted during the follow-up period from 1 to 104 months [average 48 month]; three due to valve and left atrial thrombus formation, two due to CVA from overdose of warfarin, and each one due to congestive heart failure and chronic constrictive pericarditis, Anticoagulants after prosthetic valve replacement were maintained with warfarin, dipyridamole and aspirin to the level of around 50% of normal prothrombin time in 79 cases, and Ticlopidine with aspirin in 47 cases to compare the result of each group. There were 11 major thromboembolic episodes including 3 deaths in the warfarin group. Two cases of CVA due to overdose of warfarin was noted in the warfarin group. In the ticlopidine group, there was only one left atrial thrombus confirmed at the time of autopsy. Among the survived 138 cases, nearly all cases[136 cases] were included in NYHA functional class I and II during the follow-up period. In conclusion, surgical treatment of the cardiac valve disease in 156 clinical cases revealed excellent result with acceptable operative risk and late mortality. Prevention of thrombus formation with anti-platelet aggregator Ticlopidine has better result than warfarin group presently with no specific side effect such as bleeding or gastrointestinal trouble.

• 한방재활의학과학회지
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• 제30권3호
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• pp.45-56
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• 2020

Objectives This study was designed to investigate the effects of ChondroT on thrombus in FeCl₃-induced rats. Methods We exposed FeCl₃ to rat's carotid artery to induce thrombus. Specimens were divided in 5 groups; Intact, Control, ASA10 (aspirin 10 mg/kg), CT100 (ChondroT 100 mg/kg), and CT200 (ChondroT 200 mg/kg), each n=6. We investigated thromboxane, platelet activating factor (PAF), histological change, lipid metabolism, transaminase, leukocyte, erythrocyte and thrombocyte level. Results In ASA10, CT200 groups, there was significants decrease in both thromboxane level and total cholesterol level, compared to control group and there were significant histological changes of blood vessel, compared to control group. In CT200 group, there was significant decrease in PAF level, compared to control group (p<0.05). In ASA10, CT200 groups, triglycerides level tended to decrease, compared to control group. Conclusions Based on these results, it could be suggested that ChondroT was effective on thrombus in FeCl₃-induced rats, and further study is needed to conduct a rigorous clinical research.

• Archives of Plastic Surgery
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• 제46권3호
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• pp.214-220
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• 2019

Background Microvascular anastomosis patency is adversely affected by local and systemic factors. Impaired intimal recovery and endothelial mechanisms promoting thrombus formation at the anastomotic site are common etiological factors of reduced anastomosis patency. Epigallocatechin gallate (EGCG) is a catechin derivative belonging to the flavonoid subgroup and is present in green tea (Camellia sinensis). This study investigated the effects of EGCG on the structure of vessel tips used in microvascular anastomoses and evaluated its effects on thrombus formation at an anastomotic site. Methods Thirty-six adult male Wistar albino rats were used in the study. The right femoral artery was cut and reanastomosed. The rats were divided into two groups (18 per group) and were systemically administered either EGCG or saline. Each group were then subdivided into three groups, each with six rats. Axial histological sections were taken from segments 1 cm proximal and 1 cm distal to the microvascular anastomosis site on days 5, 10, and 14. Results Thrombus formation was significantly different between the EGCG and control groups on day 5 (P=0.015) but not on days 10 or 14. The mean luminal diameter was significantly greater in the EGCG group on days 5 (P=0.002), 10 (P=0.026), and 14 (P=0.002). Intimal thickening was significantly higher on days 5 (P=0.041) and 10 (P=0.02). Conclusions EGCG showed vasodilatory effects and led to reduced early thrombus formation after microvascular repair. Similar studies on venous anastomoses and random or axial pedunculated skin flaps would also contribute valuable findings relevant to this topic.