• Biomaterials and Biomechanics in Bioengineering
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• 제2권1호
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• pp.33-45
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• 2015

To engineer tissue-like structures, cells are required to organize themselves into three-dimensional networks that mimic the native tissue micro-architecture. Here, we present agarose-based multi-well platform incorporated with electrical stimulation to build skeletal muscle-like tissues in a facile and highly reproducible fashion. Electrical stimulation of C2C12 cells encapsulated in collagen/matrigel hydrogels facilitated the formation 3D muscle tissues. Consequently, we confirmed the transcriptional upregulations of myogenic related genes in the electrical stimulation group compared to non-stimulated control group in our multi-well 3D culture platform. Given the robust fabrication, engineered muscle tissues in multi-well platform may find their use in high-throughput biological studies drug screenings.

• Applied Biological Chemistry
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• 제38권6호
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• pp.516-521
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• 1995

항 MRSA 물질을 찾기 위하여 찬국 해양 토양을 검색하였고, 거기서 분리된 방선균의 이차 대사물질 중 항 MRSA 효능을 보이는 물질을 AM3이라고 명명하고 이에 대한 연구를 하였다.

• 한국진공학회:학술대회논문집
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• 한국진공학회 2013년도 제45회 하계 정기학술대회 초록집
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• pp.274.2-274.2
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• 2013

We described a simple and efficient fabrication method for generating microfluidic channels with a circular-cross sectional geometry by exploiting the reflow phenomenon of a thick positive photoresist. Initial rectangular shaped positive photoresist micropatterns on a silicon wafer, which were fabricated by a conventional photolithography process, were converted into a half-circular shape by tuning the temperature to around $105^{\circ}C$. Through optimization of the reflow conditions, we could obtain a perfect circular micropattern of the positive photoresist, and control the diameter in a range from 100 to 400 ${\mu}m$. The resultant convex half-circular photoresist was used as a template for fabricating a concave polydimethylsiloxane (PDMS) through a replica molding process, and a circular PDMS microchannel was produced by bonding two half-circular PDMS layers. A variety of channel dimensions and patterns can be easily prepared, including straight, S-curve, X-, Y-, and T-shapes to mimic an in vivo vascular network. To inform an endothelial cell layer, we cultured primary human umbilical vein endothelial cells (HUVECs) inside circular PDMS microchannels, and demonstrated successful cell adhesion, proliferation, and alignment along the channel.

• Journal of Microbiology and Biotechnology
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• 제28권12호
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• pp.2121-2132
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• 2018

Abnormal melanin synthesis results in several hyperpigmentary disorders such as freckles, melanoderma, age spots, and other related conditions. In this study, we investigated the anti-melanogenic effects of an extract from the microalgae Chlamydomonas reinhardtii (CE) and potential mechanisms responsible for its inhibitory effect in B16F10, normal human epidermal melanocyte cells, and human skin-equivalent models. The CE extract showed significant dose-dependent inhibitory effects on ${\alpha}$-melanocyte-stimulating, hormone-induced melanin synthesis in cells. Additionally, the CE extract exhibited suppressive effects on the mRNA and protein expression of microphthalmia-associated transcription factor, tyrosinase, tyrosinase-related protein-1, and tyrosinase-related protein-2. The CE extract also inhibited the phosphorylation of protein kinase A and extracellular signal-related kinase, which function as upstream regulators of melanogenesis. Using a three-dimensional, reconstructed pigmented epidermis model, the CE-mediated, anti-pigmentation effects were confirmed by Fontana-Masson staining and melanin content assays. Taken together, CE extract can be used as an anti-pigmentation agent.

• 대한약학회:학술대회논문집
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• 대한약학회 2002년도 Proceedings of the Convention of the Pharmaceutical Society of Korea Vol.2
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• pp.366.1-366.1
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• 2002

Emerging medical technologies for effective and lasting repair of articular cartilage include delivery of cells or cell-seeded scaffolds to a defective site to initiate de novo tissue regeneration. In this respect. the availability of an appropriate biomaterial scaffold is crucial to allow chondrocyte growth and cartilaginous matrix deposition in a three-dimensional geometry. Hyaluronic acid (HA) molecules are anchored to the chondrocyte membrane via receptors, such as CD44. (omitted)

• ETRI Journal
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• 제42권6호
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• pp.837-845
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• 2020

The objective of this study was to implement an in vitro exposure system for 28 GHz to investigate the biological effects of fifth-generation (5G) communication. A signal source of 28 GHz for 5G millimeter-wave (MMW) deployment was developed, followed by a variable attenuator for antenna input power control. A power amplifier was also customized to ensure a maximum output power of 10 W for high-power 28-GHz exposure. A 3-dB uniformity over the 80 mm × 80 mm area that corresponds to four Petri dishes of three-dimensional cell cultures can be obtained using a customized choke-ring-type antenna. An infrared camera is employed for temperature regulation during exposure by adjusting the airflow cooling rate via real-time feedback to the incubator. The reported measurement results confirm that the input power control, uniformity, and temperature regulation for 28-GHz exposure were successfully accomplished, indicating the possibility of a wide application of the implemented in vitro exposure system in the fields of various MMW dose-response studies.

• 문화기술의 융합
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• 제10권1호
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• pp.345-352
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• 2024

삼성동 코엑스에 위치한 'K-Pop Squre Media'는 대형 전광판에 다양한 입체 동영상을 상영하며, 대중적 이목과 매스미디어의 주목을 받게 되었다. 기존과 차별화된 대형 곡면 LED 전광판의 영상은 미디어 파사드 기법의 일종으로 코엑스 광장의 공간적 특성과 함께 새로운 랜드마크이자 명소로 자리 잡고 있다. 이에, 본 연구는 도시 공간 속 옥외 광고에서 활용되는 미디어 파사드에 대해서 K-Pop Squre Media를 중심으로 그 특성을 살펴보고자 한다. 이를 위한 연구의 접근은 첫째, 도심의 공공 공간을 중심으로 '공간적 특성'에 대해 케빈린치(Kevin Lynch)의 도시 공간을 구성하는 5가지 요소, 월리엄 미첼(William J. Mitchell)이 제시한 스마트 스페이스와 공간 패러다임의 변화를 중심으로 살펴본다. 둘째, 옥외 광고의 '뉴미디어성'을 중심으로 Digital OOH(Out-Of-Home) Media의 세 가지 유형에 대해 분석하고 셋째, '미디어 파사드의 신기술과 내용구성'에 대해 전시 매체 (프로젝션, LED 스크린)의 유형, 미디어 파사드의 시초, 최신 전시 기술인 아나몰픽(Anamolphic)에 대해 살펴본다. 본 연구를 통해 K-Pop Squre Media는 스마트 스페이스의 공간적 특성, Digital OOH의 뉴미디어 특성, 그리고, 미디어 파사드의 내용 구성을 통해 공공성, 소통성, 예술성, 장소성의 특성을 보이고 있음을 알 수 있다.

• 복식
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• 제51권5호
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• pp.113-134
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• 2001

This study was carried out with an objective to prepare the framework of conformity for the 21st century modern fashion, which is interactive with mankind, based on a position that the 21st century contemporary fashion with its amalgamating trend of diversified artistic forms may not be elucidated on a standpoint centering on one certain modality. The hybrid fashion trend of historical modality which provides freedom and satisfaction in creation of an individuals expressive power in expressing true desire of mans inside through man-centered thought of the times has made multifarious motives of the past and present fragmentary. It thus extracts inner divisions and the concept of consolidation through three-dimensional form. Modern fashion is newly interpreted by the material and details of high technologies. and is harmonized by mix and match with various expressions. By having it, it is being expressed together with many types of dresses and ornaments. Modern fashion is being expressed together with many types of dresses and ornaments by new interpretations with the material and details of high technologies and by being harmonized with mix and match with various expressions. The hybrid fashion design trend of regional elements formed by stimuli and contacts of diverse local culture by globalization of network that was achieved by scientific technologies of the contemporary information society has disintegrated varied boundaries in the conventional culture by the latest communication technology-new media on the basis of newly created culture. The fact that regional elements could be expressed as if they were interrelated without each being deprived of originality may be explained with an interpretation on pluralism. The hybrid fashion design trend of cultural aspect, which manifests in the background of scientific technological culture as it transcends the societal-cultural boundaries based on the de-centralization theory of Frederic Jameson, has supplemented the imperfect meanings through conversional correlation with other forms and internal program changes. The middle stratal and polyhedral characteristics are seen as each cultural element is dismantled and reassembled by application of 'multiple-time point expression'. Design forming method is not to bring destruction from outside, but to embrace instability and chaos through radical dismantling of the inside. and to pursue diversity and openness. Thus. it is implemented by an approach that takes the role of design process. In communication of discontinuity, continuity was dismantled through forms of mixing, overlap, perversion, insertion and coincidence.

• 한국해양환경ㆍ에너지학회지
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• 제6권1호
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• pp.44-59
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• 2003

육상축양장 배출수 처리에 의한 대상해역의 수질개선 효과를 산정하기 위하여 생태계 모델시뮬레이션을 행하였다 가막만에서의 잔차류 패턴은 해수가 출입하는 북쪽의 협수도에서는 남향류가 강한 것으로 나타났으며, 남쪽 만구부에서는 동향류가 우세하며 시계방향의 환류가 강하게 나타났다. 9개 정점 표층에서의 DIP, DIN과 COD 농도의 지점별 계산치와 관측치를 비교한 결과 DIP의 경우 상대오차는 평균 14.3％, DIN은 평균 25.8％ 그리고 COD는 평균 14.4％였다. DIP, DIN과 COD 모두 오염부하의 영향이 큰 만의 북서쪽에서 높은 농도를 나타내었다. 육상축양장 배출수의 오존처리 시스템 처리효율을 DIP 90％, DIN 80％ 그리고 COD 60％로 적용하여 시뮬레이션 한 결과 DIP의 경우는 9개 정점에서 34.4~54.0％ 범위로 평균 46.4％의 수질 개선효과가 나타났으며, DIN의 수질 개선효과는 0.4~25.4％ 범위로 평균 8.4％로 나타났다. 그리고 COD는 만 전체적으로 농도가 감소하여 수질환경기준 I등급 영역이 확장되었으며, 9개 정점에서 15.6~29.4％ 범위로 평균 22.7％의 수질 개선효과가 나타났다.

• Journal of Microbiology and Biotechnology
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• 제32권4호
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• pp.531-540
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• 2022

Due to the high incidence of malignant melanoma, the establishment of in vitro models that recapitulate the tumor microenvironment is of great biological and clinical importance for tumor treatment and drug research. In this study, 3D printing technology was used to prepare GelMA/PEGDA composite scaffolds that mimic the microenvironment of human malignant melanoma cell (A375) growth and construct in vitro melanoma micro-models. The GelMA/PEGDA hybrid scaffold was tested by the mechanical property, cell live/dead assay, cell proliferation assay, cytoskeleton staining and drug loading assay. The growth of tumor cells in two- and three-dimensional culture systems and the anti-cancer effect of luteolin were evaluated using the live/dead staining method and the Cell Counting Kit-8 (CCK-8) method. The results showed a high aggregation of tumor cells on the 3D scaffold, which was suitable for long-term culture. Cytoskeleton staining and immunofluorescent protein staining were used to evaluate the degree of differentiation of tumor cells under 2D and 3D culture systems. The results indicated that 3D bioprinted scaffolds were more suitable for tumor cell expansion and differentiation, and the tumor cells were more aggressive. In addition, luteolin was time- and dose-dependent on tumor cells, and tumor cells in the 3D culture system were more resistant to the drug.