• Applied Biological Chemistry
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• v.30 no.1
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• pp.88-94
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• 1987

To investigate ginseng oligopeptides with biological activities, the water extract was purified by ultra-filtration, gel filtration, ion-exchange and thin layer chromatography. Ultra-filtered water extract exhibited antilipolytic activity, inhibiting epinephrine-induced lipolysis in the isolated fat cells of rat epididymal adipose tissue. The filtrate was separated into 3 fractions by Sephadex G-25 gel filtration. Peptides were found only in the first fraction(S-FI). Saponine and sugars were also detected in tie fraction. S-FI fraction resolved further into 6 fractions by Dowex 50 ion-exchange chromatography. The sugar and saponine depleted fraction(P-F2) from the second chromatography showed antilipolytic activity. The P-F2 fraction revealed 6 spots on TLC. The 6 spots were isolated by TLC and identified as peptides.

• Journal of Integrative Natural Science
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• v.3 no.4
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• pp.215-218
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• 2010

cis,cis-1,2,3,4-Tetraphenylbutadiene has been synthesized and its optical properties are investigated by using UV-Vis absorption and fluorescence spectroscopy. Thin films of tetraphenylbutadiene prepared from thin layer chromatography(TLC) displays strong luminescence and used for the detection of vapor of organic halide. Tetraphenylbutadiene shows dramatic quenching photoluminescence under exposure of chloroform vapor.

• Journal of the Korean Society of Food Science and Nutrition
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• v.25 no.6
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• pp.1069-1073
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• 1996

Inhibition of xanthine oxidase by seaweed extracts obtained from Undaria pinnatifida, Ecklonia stolonifera, Ecklonia cava, Laminaria japonica, Sargassum, Codiumfragile, Enteromorpha compressa and Porphyra tenera were investigated. Extracts of E. stolonifera and E. mua remarkably inhibited xanthine oxidase activity compared to those of other seaweed. The xanthine oxidase inhibitory activity of E. cava was higher than that of E. stolonifera. Diethyl ether extract from E. cava was more effective in the inhibition of xanthine oxidase than other solvent extracts. Two xanthine oxidase inhibitors(A-1 and A-2) from diethyl ether extract were isolated and purified by silica gel column chromatography, thin layer chromatography and high performance liquid chromatography. Xanthine oxidase inhibitory activities of these compounds were 27.8 and 48.1% per 0.4mg, respectively. The active compound A-2 had absorption peak at 420nm, 456nm and 467nm, which can be considered as siphonaxanthine.

• The Korean Journal of Food And Nutrition
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• v.13 no.2
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• pp.146-151
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• 2000

To evaluate anticaries and antiinflammation of Aloe vera peel, antimicrobial substances were extracted from Aloe vera peel and identified. The antimicrobial active substances of water extract were successfully purified with solvent fractionation, silica gel column chromatography, preparative thin layer chromatography and UV spectrophotometer. Two purified active substances were identified as aloe-emodin and barbaloin by Mass Spectrometer, 1H-NMR and FT-IR.

• Korean Journal of Microbiology
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• v.11 no.3
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• pp.109-114
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• 1973

It is well known that the ornithine-containing lipids were separated and identified from the other lipids of Rhodopseudomonas spheroides, Rhodospirillum, and Thiobacillus thiooxidans. An ornithine-containing lipid that lacks phosphorus and glycerol has been observed in Ferrobacillus ferrooxidans which was isolated from dalsung copper mine in Korea. The aminolipid was extracted from F.ferrooxidans and furthr purified by thin layer chromatography, and the product was identified as an ornithine-containing lipid by paper and liquid chromatography respectively.

• Korean Journal of Microbiology
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• v.23 no.4
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• pp.271-281
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• 1985

Bacillus thuringiensis var. thuringiensis produces an extracellular insecticidal thermostable .betha.-exotoxin, which was purified through microfiltering, barium precipitation, charcoal absorption chromatography, ion exchange column chromatography and gel filtration. The exotoxin in each purification step was detedted by thin layer chromatography, high pressure liquid chromatography and paper electrophoresis with efficient results. The exotoxin productivity on time course was checked by spectrophotometric absorbance at 258nm with the result that the exotoxin was initially produced in 6 hour culture and reached maximum value in 36 hour culture. Anti-bacterial effect test on Micrococcus flava was applied as toxicity test. The results showed that frowth inhibition of M. flava could be shown in plate assay of cell free filtered supernatant, alkaline eluant from charcoal and purified exotosin obtained from gel filtration column chromatography on Sephadex G-10 appeared to be 740. Heat stability of the exotoxin was confirmed through autoclaving twice.

• Applied Biological Chemistry
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• v.29 no.3
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• pp.318-323
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• 1986

We empolyed gel filtraction, polyacrylamide gel electrophoresis, amino acid autoanalyzer, thin layer chromatography for determining protein and lipid composition in walnut. The walnut contained 22.18% of crude protein and 64.23% of crude lipid. Glutamic acid (38.60%) was the major amino acid in soluble protein, followed by arginine and aspartic acid. The sodium dodecyl sulfate polyacrylamide gel electrophoresis analysis showed 12 band in soluble protein of walnut, and collection rate of main protein fraction purified by Sephadex G-150 was 60.67%. The molecular weight for the main protein was estimated to be 43,000. The lipid fraction obtained by silicic acid column chromatography were mainly composed of about 93.05% neutral lipid, whereas compound lipid was only 7.0% level. Among the neutral lipid by thin layer chromatography, triglyceride was 82.05%, sterol ester and free fatty acid were 3.86% and 4.80%, repectively. The predominant fatty acids of total and neutral lipids were linoleic acid $(64.48{\sim}69.98%)$ and oleic acid $(13.89{\sim}15.36%)$. The major fatty acids of triglyceride separated from neutral lipid were linolenic acid (69.98%).

• Proceedings of the Costume Culture Conference
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• 2004.04a
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• pp.44-46
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• 2004

심하게 퇴색된 출토 복식유물의 경우 유물의 고유색을 알지 못함은 물론 사용된 염료의 종류를 알지 못함으로 인하여 유물의 보존처리 및 장기간 보관과 전시에 큰 어려움을 지닌다. 이화학 분야에서는 미지시료의 기초 성분분석 방법으로서 thin layer chromatography(TLC)법이 오래 전부터 사용되었는데, 퇴색된 복식유물의 염료분석을 다룬 일부 선행연구에서는 이를 활용하여 유물의 염료를 판정하였다(Kharbade ＆ Agrawal, 1985; Schweppe, 1989). (중략)

• 한국생물공학회:학술대회논문집
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• 2003.10a
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• pp.300-300
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• 2003

• Korean Journal of Food Science and Technology
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• v.36 no.4
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• pp.655-661
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• 2004

Sphingolipid production was investigated through Ganoderma lucidum-submerged cultivation. Crude sphingolipid obtained from G. lucidum was purified by methanol precipitation, Dowex AG DW-X8 (H+ form) cation exchange chromatography, and preparative thin layer chromatography, Structure and functionalities of purified sphingolipid were elucidated including cutaneous hydration effect. Possibility of use as cosmetics material and new biomaterial was explored. Production was 0.4 g/L at 1% yield. Purified sphingolipid was identified as D-ribo-1,3,4-trihydroxy-2-aminoocta decan through UV/VIS, FT-IR, and $^1H-NMR$. Sphingolipids increased skinmate value for cutaneous hydration effect by 20% at $500\;{\mu}g/mL$ and decreased skin roughness at $100\;{\mu}g/mL$. Results suggest shingolipids from G. lucidum are effective for cutaneous hydration and improvement of skin roughness.