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Histological and Histochemical Studies on the Cutaneous Mucous Glands According to the Development of Frog, Rana nigromaculata (개구리(Rana nigromaculata)발생에 따른 피부점액선의 조직학적 및 조직화학적 연구)

  • Kim, Han-Hwa;Noh, Yong-Tai;Chung, Young-Wha
    • The Korean Journal of Zoology
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    • v.18 no.2
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    • pp.51-60
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    • 1975
  • The present study was performed histologically and histochemically to observe the cutaneous mucous glands in the frog, Rana nigromaculata during metamorphosis. The cutaneous thssues including dermal plicae in the dorsal portions of the frog tadpoles at each metamorphosis stage were fixed in 10% buffered formalin at$4^{\circ}C$, embedded in paraffin wax, sectioned 4 $\mu$m thickness and stained with periodic acid-Schiff(PAS) and alcian blue (AB) at both pH 2.5 and pH 1.0. The results of observation were as follows: 1. The developments of cutaneous mucous glands of the frog tadpole were begun with appearance of gland cell nest in the dermis at metamorphosis XV stage and significant numerical increases could be seen at metamorphosis XX, XXIII and XXIV stages. 2. This cutaneous mucous gland of the frog tadpole could be divided into two types; A-type glands showed strong positivities to both PAS and AB at pH 2.5 in the gland body cells and to PAS in the gland neck cells, and B-type glands at AB pH 2.5 in the gland body cells. 3. In the A-type mucous glands, the reactivities of the glandular epithelial cells to both PAS and AB stain could be first seen at the metamorphosis XIX stage of frog tadpole. The reactivities of the glandular epithelial cells to both PAS and AB pH 2.5 were gradually increased according to the process of metamorphosis after XX stage of metamorphosis. 4. The B-type mucous glands were first seen at the XX stage and the reactivity of the glandular epithelial cells to AB at pH 2.5 was gradually increased according to the process of metamorphosis after XX stage. 5. The A-type and the B-type mucous glands were in the ratio of 99 : 1, 7 : 3 and 5.5 : 4.5 for each of metamorphosis XX, XXI-XXII and XXIII-XXV stages. 6. The remarkable development of the cutaneous mucous glands of the frog tadpoles might be needed to maintain water and electrolyte balances according to the change of way from aquatic life to amphibious.

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One-Dimensional Consolidation Simulation of Kaolinte using Geotechnical Online Testing Method (온라인 실험을 이용한 카올리나이트 점토의 일차원 압밀 시뮬레이션)

  • Kwon, Youngcheul
    • KSCE Journal of Civil and Environmental Engineering Research
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    • v.26 no.4C
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    • pp.247-254
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    • 2006
  • Online testing method is one of the numerical experiment methods using experimental information for a numerical analysis directly. The method has an advantage in that analysis can be conducted without using an idealized mechanical model, because mechanical properties are updated from element test for a numerical analysis in real time. The online testing method has mainly been used for the geotechnical seismic engineering, whose major target is sand. A testing method that may be applied to a consolidation problem has recently been developed and laboratory and field verifications have been tried. Although related research thus far has mainly used a method to update average reaction for a numerical analysis by positioning an element tests at the center of a consolidation layer, a weakness that accuracy of the analysis can be impaired as the thickness of the consolidation layer becomes more thicker has been pointed out regarding the method. To clarify the effectiveness and possible analysis scope of the online testing method in relation to the consolidation problem, we need to review the results by applying experiment conditions that may completely exclude such a factor. This research reviewed the results of the online consolidation test in terms of reproduction of the consolidation settlement and the dissipation of excess pore water pressure of a clay specimen by comparing the results of an online consolidation test and a separated-type consolidation test carried out under the same conditions. As a result, the online consolidation test reproduced the change of compressibility according effective stress of clay without a huge contradiction. In terms of the dissipation rate of excess pore water pressure, however, the online consolidation test was a little faster. In conclusion, experiment procedure needs to improve in a direction that hydraulic conductivity can be updated in real time so as to more precisely predict the dissipation of excess pore water pressure. Further research or improvement should be carried out with regard to the consolidation settlement after the end of the dissipation of excess pore water pressure.

Structural Evaluation Method to Determination Safe Working Load of Block Handling Lugs (블록 이동용 러그의 안전사용하중 결정에 관한 구조 평가법)

  • O-Hyun Kwon;Joo-Shin Park;Jung-Kwan Seo
    • Journal of the Korean Society of Marine Environment & Safety
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    • v.29 no.4
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    • pp.363-371
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    • 2023
  • To construct a ship, blocks of various sizes must be moved and erected . In this process, lugs are used such that they match the block fastening method and various functions suitable for the characteristics of each shipyard facility. The sizes and shapes of the lugs vary depending on the weight and shape of the block structures. The structure is reinforced by welding the doubling pads to compensate for insufficient rigidity around the holes where the shackle is fastened. As for the method of designing lugs according to lifting loading conditions, a simple calculation based on the beam theory and structural analysis using numerical modeling are performed. In the case of the analytical method, a standardized evaluation method must be established because results may differ depending on the type of element and modeling method. The application of this ambiguous methodology may cause serious safety problems during the process of moving and turning-over blocks. In this study , the effects of various parameters are compared and analyzed through numerical structural analysis to determine the modeling conditions and evaluation method that can evaluate the actual structural response of the lug. The modeling technique that represents the plate part and weld bead around the lug hole provides the most realistic behavior results. The modeling results with the same conditions as those of the actual lug where only the weld bead is connected to the main body of the lug, showed a lower ulimated strength compared with the results obtained by applying the MPC load. The two-dimensional shell element is applied to reduce the modeling and analysis time, and a safety working load was verified to be predicted by reducing the thickness of the doubling pad by 85%. The results of the effects of various parameters reviewed in the study are expected to be used as good reference data for the lug design and safe working load prediction.

Effect of Restriction of Vitamin A and D on Carcass Characteristics in Hanwoo Steers (비타민 A와 D의 공급제한이 거세 한우의 육질등급에 미치는 영향)

  • Kim, W.Y.;Park, J.K.;Cho, S.Y.;Nam, K.T.;Yeo, J.M.
    • Journal of Practical Agriculture & Fisheries Research
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    • v.18 no.1
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    • pp.13-24
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    • 2016
  • Sixty Hanwoo steers(15 months of age; 409±29.2 kg of BW) were used to evaluate the effects of dietary vitamins A and D restriction on carcass characteristics. Steers were allotted randomly to 1 of 4 treatments: Control(diet supplemented with vitamins A, D and E), -A (diet supplemented with vitamins D and E), -D(diet supplemented with vitamins A and E) and -AD(diet supplemented with vitamin E only). Steers were fed the experimental diet for a period of 8 months(until 23 months of age), and then supplemented with vitamins A and D at 0.05% of the diet(as fed-basis) from 24 to 26 months of age, and at 0.1% of the diet from 27 to 31 months of age(harvesting time). Dietary restriction of vitamins A and D did not affect DM intake, daily gain and feed conversion ratio. But the concentration of serum retinol was significantly(P<0.05) decreased by vitamin A restriction with the lowest concentration being seen at 23 months of age(345.0 ㎍/L and 326.7㎍/L for control and -D treatment versus 169.3 ㎍/L and 175.4 ㎍/L for -A and -AD treatments). The serum concentration of 25(OH)D3 was also decreased significantly(P<0.05) by vitamin D restriction and the lowest concentration was seen at 18 months of age(53.7ng/ml and 61.8ng/ml for control and - A treatment versus 24.0 ng/ml and 24.5 ng/ml for -D and -AD treatments). After the restriction period of vitamins A and D, the concentrations of retinol and 25(OH)D3 for - A, -D and -AD treatments were recovered at those of control. Dietary restriction of vitamins A and D did not affect carcass weight, backfat thickness, ribeye area, quality grade and yield grade. But marbling score was significantly increased by vitamin A restriction compared with control(6.73, 6.87 and 5.73 for -A, -AD and control, respectively). The results of the present study suggested that dietary vitamin A restriction could improve marbling score in Hanwoo steers.

An Understanding the Opening Style of the West Philippine Basin Through Multibeam High-Resolution Bathymetry (고해상도 다중빔음향측심 지형자료 분석을 통한 서필리핀분지의 진화 연구)

  • Hanjin Choe;Hyeonuk Shin
    • Journal of the Korean earth science society
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    • v.44 no.6
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    • pp.643-654
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    • 2023
  • The West Philippine Basin, an oceanic basin half the size of the Philippine Sea Plate, lies in the western part of the plate and south of the Korean Peninsula on the Eurasian Plate. It subducts beneath the Eurasian Plate and the Philippine Islands bordering the Ryukyu Trench and the Philippine Trench with 25-50% of this basin already consumed. However, the history of the opening of the basin's southern region has been a topic of debate. The non-transform discontinuity formed during the seafloor spreading is similar to the transform fault boundaries normally perpendicular to mid-ocean ridge axes; however, it was created irregularly due to ridge propagations caused by variations of mantle convection attributable to magma supply changes. By analyzing high-resolution multi-beam echo-sounding data, we confirmed that the non-transform discontinuity due to the propagating rift evolved in the entire basin and that the abyssal hill strike direction changed from E-W to NNW-SSE from the fossil spreading center. In the early stage of basin extension, the Amami-Sankaku Basin was rotated 90 degrees clockwise from its current orientation, and it bordered the Palau Basin along the Mindanao Fracture Zone. The Amami-Sankaku Basin separated from the Palau Basin while the spreading of the West Philippine Basin began with a counter-clockwise rotation. This indicates that the non-transform discontinuities formed by a sudden change in magma supply due to the drift of the Philippine Sea Plate and simultaneously with the rapid changes in the spreading direction from ENE-WSW to N-S. The Palau Basin was considered to be the sub-south of the West Philippine Basin, but recent studies have shown that it extends into an independent system. Evidence from sediment layers and crustal thickness hints at the possibility of its existence before the West Philippine Basin opened, although its evolution continues to be debated. We performed a combined analysis using high-resolution multi-beam bathymetry and satellite gravity data to uncover new insights into the evolution of the West Philippine Basin. This information illuminates the complex plate interactions and provides a crucial contribution toward understanding the opening history of the basin and the Philippine Sea Plate.

Non-Destructive Material Analysis of Whetstones Discovered in Grain Transport Ship of the Early Joseon Period (조선 초기 조운선(마도4호선)에서 출수된 숫돌의 비파괴 재질 분석 연구)

  • Dal-Yong Kong;Jae Hwan Kim;Eun Young Park;Yong Cheol Cho;Ki Hong Yang
    • Economic and Environmental Geology
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    • v.56 no.6
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    • pp.661-674
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    • 2023
  • From the seafloor of Taean, Chungcheongnamdo Province, a ship of the Joseon Dynasty was discovered for the first time in the history of underwater excavations in Korea in 2014 and was named Mado Shipwreck No. 4. A total of 27 unused whetstones loaded as tribute were discovered on the hull of Mado No. 4, which revealed that Mado Shipwreck No. 4 was a Grain transport ship that sank while carrying tribute from Naju to Hanyang between 1417 and 1425 (King Taejong to King Sejong). All of the 27 whetstones are in the shape of narrow and long sticks. The average values of length, width, thickness, and weight are 161.5 mm, 36.1 mm, 22.7 mm, and 253.2 g, respectively. The result of X-ray diffraction analysis shows that the constituent minerals are quartz, alkali feldspar, and plagioclase, which is similar to that of the high-resolution digital stereomicroscope analysis. The average porosity of Mado-2672 and 2673 is 2.69% and 1.78%, respectively, and the average surface hardness is 807.2HLD and 834.5HLD, respectively. It is interpreted that if the porosity increases beyond a certain level, it affects the decrease in surface hardness. All of these are made of feldspathic sandstones with an average SiO2 content of 74.51% and were confirmed to be suitable as grindstones. They are all medium whetstones when classified based on the SiO2 content. These whetstones are small in size and weight and are convenient to carry, so they are presumed to be a type of non-stationary whetstone, and are estimated to have been mainly used in the fields such as weapon polishing and craft production during the Joseon Dynasty.

Evaluation of Virtual Grid Software (VGS) Image Quality for Variation of kVp and mAs (관전압과 관전류량 변화에 대한 가상 그리드 소프트웨어(VGS) 화질평가)

  • Chang-gi Kong
    • Journal of the Korean Society of Radiology
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    • v.17 no.5
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    • pp.725-733
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    • 2023
  • The purpose of this study is to evaluate the effectiveness of virtual grid software (VGS). The purpose of this study is to evaluate the changes in energy and object thickness by dividing the use of VGS into two cases (Without-VGS) without using a movable grid. We attempted to determine the effectiveness of VGS by acquiring images using a chest phantom and a thigh phantom and analyzing SNR and CNR. In the chest phantom and femoral phantom, the tube flow was fixed at 2.5 mAs, and the tube voltage was changed by 10 kVp from 60 to 100 kVp to measure SNR and CNR, and SNR was about 1.09 to 8.86% higher in the chest phantom than in Without-VGS, and CNR was 4.18 to 14.56% higher in the VGS than in Without-VGS. And in the femoral phantom, SNR was about 9.78 to 18.05% higher in VGS than in Without-VGS, and CNR was 21.07 to 44.44% higher in VGS than in Without-VGS. The tube voltage was fixed at 70 kVp in the chest phantom and the femoral phantom, and the amount of tube current was changed at 2.5 to 16 mAs, respectively, and after X-ray irradiation, SNR and CNR were measured in the chest phantom, which was about 1.49 to 11.11% higher in VGS than in Without-VGS, and CNR was 4.76 to 13.40% higher in VGS than in Without-VGS. And in the femoral phantom, SNR was about 2.22 to 17.38% higher in VGS than in Without-VGS, and CNR was 13.85 to 40.46% higher in VGS than in Without-VGS. Therefore, if an inspection is required with a mobile X-ray imaging device, it is believed that good image quality can be obtained by using VGS in an environment where it is difficult to use a mobile grid, and it is believed that the use of mobile X-ray devices can be increased.

Effects of Crude Protein and Calcium Levels in Feed on Growth Performance and Carcass Characteristic of Hanwoo Steers (사료 내 조단백질 및 칼슘 수준에 따른 비육후기 거세한우의 성장성적 및 도체특성에 미치는 영향)

  • Ryu, Chae-Hwa;Lee, Sung-Dae;Lee, Seul;Baek, Youl-Chang
    • Korean Journal of Organic Agriculture
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    • v.31 no.4
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    • pp.453-460
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    • 2023
  • This study was conducted to confirm the growth performance of Hanwoo steers according to the crude protein and calcium levels and their effects on carcass characteristics. The experiment consisted of a total of 4 groups: HPHC (CP 15%, Ca 1.2%), HPLC (CP 15%, Ca 0.6%), LPHC (CP 12.5%, Ca 1.2%), LPLC (CP 12.5%, Ca 0.6%). In the feeding trial, Hanwoo steers (650.8±16.27 kg, n = 32, 24 months of age) were divided into four feeding groups (n = 8 each) with the same initial body weight. Animals were fed with each rice straw and concentrate (1:9) until the late fattening stage. Growth performance was measured by daily weight gain, feed conversion rate, and final weight. Carcass characteristics were measured carcass weight, loin area, back-fat thickness and marbling score. There was no significant difference in growth performance and carcass characteristics among the treatment groups. However, the ratio of meat quality grade 1+ or higher was 76% in LPLC, which was higher than other treatments (HPHC, 63%; HPLC, 63% and LPHC, 50%). Therefore, reducing crude protein in the feed did not affect growth and carcass characteristics. In addition, calcium control in the late fattening stage did not affect growth performance. In LPLC, the ratio of meat quality 1+ or higher was the highest, so additional research on calcium control in feed is thought to be necessary. However, there was no significant difference in the relationship between protein and calcium levels in feed in all study results, so the influence of the two factors on each other is thought to be low.

A Study on the Radioprotection Effect of Selenium and Arginine Mixtures for Reducing Radiation Damage to Police SOU (경찰특공대 요원의 방사선손상 감소를 위한 셀레늄과 아르기닌 혼합물의 방호효과 연구)

  • Geun-Woo Jeong;Hae-Suk Kim;Jae-Hyeong Park;Sung-Hyun Joo;Jae-Gyeong Choi;Se-Im Cheon;Byung-In Min
    • Journal of the Korean Society of Radiology
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    • v.18 no.2
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    • pp.119-125
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    • 2024
  • The purpose of this study is to examine the radioprotection effect of mixtures of selenium and arginine for development of radioprotection agents that can minimize radiation damage to police special operation unit in the event of radioactive terrorism. In this study 72 male rats were classified into 4 groups: normal group(NC Group), selenium and arginine mixtures administration group(SeAr Group), radiation exposure group(IR Group), and selenium and arginine mixture administration group followed by radiation exposure(SeAr+IR Group). The 7Gy of X-ray was irradiated to whole body of SD rats. And selenium and arginine were dministered orally at 3mg/kg and 150mg/kg once a day for 14 days. And then hematological and histological analyzes were performed on days 1, 7, and 21 after radiation exposure. In hemotological analysis, significant radioprotection wes observed in lymphocytes(p<0.05) on day 1, platelet(p<0.01) on day 7, red blood cell(p<0.01) on day 21 of radiation exposure in SeAr+IR group compared to IR group. In histological analysis, it was observed that the border of small crypt cells in the small intestine was less collapsed and the length of small crypts was relatively recovered on day 7 and showed that the number of cells and cell wall thickness were better in the prostate on day 21 in SeAr+IR group compared to IR group. Therefore, it is judged that selenium and arginine mixtures have radioprotection effect on blood and tissues due to radiation exposure. it will be helpful for research on radioprotection agents to reduce radiation damage to police special operation unit.

Studies on the MSM (Methyl Sulfonyl Methane) Treatment Method of Soybeans (콩의 MSM (Methyl Sulfonyl Methane) 처리 방법에 관한 연구)

  • Se Eun Chae;Seung Ka Oh;Young-Son Cho;Doobo Shim;Dong-Kyung Yoon;Seung Ho Jeon
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.69 no.1
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    • pp.25-33
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    • 2024
  • This experiment was conducted at the affiliated farm of the Suncheon University from 2022 to 2023 to investigate the growth, yield, and quality characteristics of soybeans based on the treatment method of Methyl Sulfony Methane (MSM) for the establishment of stable production practices. In the initial investigation of growth characteristics in 2022, an increasing trend in characteristics such as plant height, stem thickness, and branching index was observed as the treatment concentration increased from 50% to 200%. Yield components also followed the same trend, with the basal fertilization + top dressing 3 times at 200% treatment showing the highest yield at 355 kg·10a-1, with the highest number of pods. In the subsequent study to determine the optimal concentration exceeding 200% in 2023, growth characteristics showed a trend of 400% > 200% > 800%. The basal fertilization + top dressing 3 times at 400% treatment exhibited the longest plant height (106.7 cm) and the most branches (6). In terms of seed quality, this treatment also had the highest proportion (66.9%) of seeds with a diameter over 6.7 mm. Additionally, in yield components such as pods, seeds per pod, and 100-seed weight, the basal fertilization + top dressing 3 times at 400% treatment showed the highest values, resulting in a maximum yield of 374 kg·10a-1, representing a 23.4% increase compared to the control. Therefore, for the optimal production of high-quality soybeans, it is recommended to apply the treatment of basal fertilization + top dressing 3 times at 400% concentration, with top dressing occurring at 30-day intervals before harvest.