• Journal of Life Science
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• v.29 no.5
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• pp.607-613
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• 2019

The purpose of this study was to investigate the effects of either chrysin or exercise on the inflammasome and thermogenic markers in the livers of high-fat fed mice. C57BL/6 mice were randomly assigned to four groups: normal diet control (NC; n=5), high-fat diet control (HC; n=5), high-fat diet with chrysin (Hch; n=5), and high-fat diet with moderate exercise (HME; n=5). The mice were fed a high-fat diet (60% of calories from fat) or normal diet (18% of calories from fat). Chrysin was supplemented orally as 50mg/kg/day dissolved in a 0.1ml solution of dimethyl sulfoxide. The exercised mice ran on a treadmill at 12-20 m/min for 30-60 min/day, 5 times/week, for 16 weeks. After the intervention, the epididymal fat and liver weights were significantly decreased in the HME group compared with HC and Hch groups. The adipocyte size was effectively decreased in the Hch and HME groups compared with the HC group. The inflammasome markers NLRP3, $IL-1{\beta}$, and caspase1 were significantly decreased in the Hch and HME groups compared with the HC group. The thermogenic markers $PGC-1{\alpha}$ and BMP7 were significantly lower in the HC than in the NC group. However, the HME group showed an increase in the thermogenic markers. In conclusion, chrysin and moderate exercise have positive effects on obese metabolic complications induced by high-fat diets by reducing inflammasome genes. However, chrysin supplementation had no effect on thermogenic gene expression. Moderate exercise would therefore seem to be more effective in controlling obesity-induced metabolic deregulation.

• Journal of Ginseng Culture
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• v.3
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• pp.119-131
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• 2021

The purpose of this study was to explore the characteristics of and solution to thermogenic reactions to Korean ginseng through bibliographic research. A review of bibliography on the thermogenic reaction induced by ginseng indicated that people who lack fluids, have fever in lungs, a stuffy chest, coughing up sputum, a strong pulse, and constipation due to fever in the stomach may exhibit such a reaction. In the herbal medicine book of the Qing Dynasty, Bon-Cho-Jeong-Ui (本草正義) recorded that wild simulated ginseng is slightly cold and replenishes the body's liquid reserves. The book also describes the field cultivated ginseng is slightly warm and enhancing stamina. Bon-Kyung-Bong-Won (本經逢源), Bon-Cho-Pyon-Dok (本草便讀), and Bon-Cho-Jeong-Ui were published authors in the Qing Dynasty, and they explained that ginseng fine root is bitter, supplements bodily fluids, and can be easily ingested because it is cold. Wol-Ji-In-Sam-Jeon (月池人蔘傳) and Bon-Cho-Bi-Yo (本草備要) described fresh ginseng assweet, bitter, and slightly cold in the Ming and Qing Dynasties, respectively. On the basis of this bibliographic description, the thermogenic reaction induced by Korean ginseng can be overcome by using ginseng fine root, fresh ginseng, or wild simulated ginseng. These ginseng radices are slightly cool, replenish human bodily fluids, and show the physiological effects of easy ingestion properties.

• 한국신재생에너지학회:학술대회논문집
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• 2007.06a
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• pp.531-533
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• 2007

We report and discuss molecular and isotopic properties of hydrate-bound gases from 55 samples and void gases from 494 samples collected during Ocean Drilling Program (ODP) Leg 204 at Hydrate Ridge offshore Oregon. Gas hydrates appear to crystallize in sediments from two end-member gas sources (deep allochthonous and in situ) as mixtures of different proportions. In an area of high gas flux at the Southern Summit of the ridge (Sites 1248-1250), shallow (0-40 meters below the seafloor (mbsf)) gas hydrates are composed of mainly allochthonous mixed microbial and thermogenic methane and a small portion of thermogenic C2+ gases, which migrated vertically and laterally from as deep as 2-2.5 km depths. In contrast, deep (50-105 mbsf) gas hydrates at the Southern Summit (Sites 1248 and 1250) and on the flanks of the ridge (Sites 1244-1247) crystallize mainly from microbial methane and ethane generated dominantly in situ. A small contribution of allochthonous gas may also be present at sites where geologic and tectonic settings favor vertical gas migration from greater depth (e.g., Site 1244).

• Molecules and Cells
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• v.45 no.10
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• pp.673-684
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• 2022

The past two decades have witnessed an upsurge in the appreciation of adipose tissue (AT) as an immunometabolic hub harbouring heterogeneous cell populations that collectively fine-tune systemic metabolic homeostasis. Technological advancements, especially single-cell transcriptomics, have offered an unprecedented opportunity for dissecting the sophisticated cellular networks and compositional dynamics underpinning AT remodelling. The "re-discovery" of functional brown adipose tissue dissipating heat energy in human adults has aroused tremendous interest in exploiting the mechanisms underpinning the engagement of AT thermogenesis for combating human obesity. In this review, we aim to summarise and evaluate the use of single-cell transcriptomics that contribute to a better appreciation of the cellular plasticity and intercellular crosstalk in thermogenic AT.

• BMB Reports
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• v.53 no.3
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• pp.142-147
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• 2020

Lipid accumulation in white adipose tissue is the key contributor to the obesity and orchestrates numerous metabolic health problems such as type 2 diabetes, hypertension, atherosclerosis, and cancer. Nonetheless, the prevention and treatment of obesity are still inadequate. Recently, scientists found that brown adipose tissue (BAT) in adult humans has functions that are diametrically opposite to those of white adipose tissue and that BAT holds promise for a new strategy to counteract obesity. In this study, we evaluated the potential of sinapic acid (SA) to promote the thermogenic program and lipolysis in BAT. SA treatment of brown adipocytes induced the expression of brown-adipocyte activation-related genes such as Ucp1, Pgc-1α, and Prdm16. Furthermore, structural analysis and western blot revealed that SA upregulates protein kinase A (PKA) phosphorylation with competitive inhibition by a pan-PKA inhibitor, H89. SA binds to the adenosine triphosphate (ATP) site on the PKA catalytic subunit where H89 binds specifically. PKA-cat-α1 gene-silencing experiments confirmed that SA activates the thermogenic program via a mechanism involving PKA and cyclic AMP response element-binding protein (CREB) signaling. Moreover, SA treatment promoted lipolysis via a PKA/p38-mediated pathway. Our findings may allow us to open a new avenue of strategies against obesity and need further investigation.

• Journal of Nutrition and Health
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• v.57 no.2
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• pp.171-184
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• 2024

Purpose: Although activating thermogenic adipocytes is a promising strategy to reduce the risk of obesity and related metabolic disorders, emerging evidence suggests that it is difficult to induce adipocyte thermogenesis in obesity. Therefore, this study aimed to investigate the regulation of adipocyte thermogenesis in diet-induced obesity. Methods: Adipose progenitor cells were isolated from the white and brown adipose tissues of control diet (CD) or high-fat diet (HFD) fed mice, and fully differentiated white and brown adipocytes were treated with β-agonists or 18-carbon fatty acids for β-adrenergic activation or peroxisome proliferator-activated receptor (PPAR) activation. Results: Compared to the CD-fed mice, the expression of uncoupling protein 1 (Ucp1) was lower in the white adipose tissue of the HFD-fed mice; however, this was not observed in the brown adipose tissue. The expression of peroxisome proliferator-activated receptor gamma (Pparg) was lower in the brown adipose progenitor cells isolated from HFD-fed mice than in those isolated from the CD-fed mice. Norepinephrine (NE) treatment exerted lesser effect on peroxisome proliferator-activated receptor-γ coactivator (Pgc1a) upregulation in white adipocytes derived from HFD-fed mice than those derived from CD-fed mice. Regardless which 18-carbon fatty acids were treated, the expression levels of thermogenic genes including Ucp1, Pgc1a, and positive regulatory domain zinc finger region protein 16 (Prdm16) were higher in the white adipocytes derived from HFD-fed mice. Oleic acid (OLA) and γ-linolenic acid (GLA) upregulated Pgc1a expression in white adipocytes derived from HFD-fed mice. Brown adipocytes derived from HFD-fed mice had higher expression levels of Pgc1a and Prdm16 compared to their counterparts. Conclusion: These results indicate that diet-induced obesity may downregulate brown adipogenesis and NE-induced thermogenesis in white adipocytes. Also, HFD feeding may induce thermogenic gene expression in white and brown primary adipocytes, and OLA and GLA could augment the expression levels.

• Advances in nano research
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• v.9 no.2
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• pp.123-131
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• 2020

Conventional fabrics that have modified in to conductive fabrics using conductive nanomaterials have novel applications in different fields. These of fabrics can be used as heat generators with the help of the Joule heating mechanism, which is applicable in thermal therapy and to maintain the warmth in cold weather conditions in a wearable manner. A modified fabric can also be used as a sensor for body temperature measurements using the variation of resistance with respect to the body temperature deviations. In this study, polyol synthesized silver nanowires (Ag NWs) are incorporated to commercially available cotton fabrics by using drop casting method to modify the fabric as a thermogenic temperature sensor. The variation of sheet resistance of the fabrics with respect to the incorporated mass of Ag NWs was measured by four probe technique while the bulk resistance variation with respect to the temperature was measured using a standard ohm meter. Heat generation profiles of the fabrics were investigated using thermo graphic camera. Electrically conductive fabrics, fabricated by incorporating 30 mg of Ag NWs in 25 ㎠ area of cotton fabric can be heated up to a maximum steady state temperature of 45℃, using a commercially available 9 V battery.

• Proceedings of the PSK Conference
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• 2002.10a
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• pp.262.1-262.1
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• 2002

Uncoupling proteins (UCPs) are transmembrane proton transporters present in the mitochondria. In UCPs. UCP1 and UCP3 play an important role in adaptive thermogenesis by uncoupling mitochondrial oxidation of substrates from ATP synthesis. PPARg coactivator 1(PGC-1) regulates transcriptional activity of PPARg and other nuclear receptors and controls the expression of UCPs. (omitted)

• The Korean Journal of Petroleum Geology
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• v.13 no.1
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• pp.24-29
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• 2007

The main purpose of this study is to identify the shallow gas origin in the KSSM zone. Based on the results of gas composition and isotope in the headsapace gas, the shallow gas is mainly composed of methane and carbon and deuterium isotopes (${\delta}^{13}CCH_4$ and ${\delta}DCH_4$) of methane has ranged from -93.4%o to -70.9%, and from -228%o to -199%o in each. These results imply that shallow gas has predominately biogenic source by $CO_2$ reduction rather than thermogenic. The carbon isotopic separation (${\varepsilon}_c$) between methane and carbon dioxide $(CO_2)$ has a range of 54.4 to 72.2, it also supports biogenic origin of shallow gas.

• Preventive Nutrition and Food Science
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• v.14 no.3
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• pp.173-178
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• 2009

This study was conducted to determine if supplementation with a compound composed of caffeine (50 mg), capsaicin (75 mg), sesamine (30 mg), L-carnitine (300 mg), banaba (50 mg) and lotus (10 mg) enhanced human autonomic nervous activities (ANS) associated with thermogenic sympathetic activity and fat utilization. Ten healthy college males (21.2$\pm$1.0 yr) volunteered for this experiment. Autonomic nervous activities associated with energy metabolism were examined at 30 min intervals for a total of 120-min while at rest and every 5-min during exercise at 50% of the ventilation threshold before and after intake of the compound or placebo with 100 ml of water for 10 days. In addition, heart rate variability power spectral analysis was used to assess human autonomic nervous activities. The results indicated that there were no significant differences in heart rate during rest and exercise among trials. Furthermore, the autonomic nervous activity tended to increase after 10-days of consumption of the test compounds during the experimental period, but the differences did not reach statistical significance. However, before and after the compound test trial there was a significantly higher respiratory gas exchange ratio (rest 0: 0.83$\pm$0.01 vs. rest 3: 0.89$\pm$0.02, p<0.05), carbohydrate oxidation (CHO) rate (rest 0: 44.57$\pm$5.83 vs. rest 2: 63.86$\pm$5.91%, p<0.05) and a lower fat oxidation rate (rest 0: 55.43$\pm$5.83 vs. rest 2: 36.14$\pm$5.91%, p<0.05. In conclusion, the results of the present study suggested that the compound composed of caffeine, capsaicin, sesamine, L-carnitine, banaba and lotus components that was evaluated in this study did not induce a significant increase in human autonomic nervous activities or lipolysis, even though the individual components have been reported to induce increased fat oxidation.