To understand contribution of thermophilic microorganisms in decomposition of litter deposits on shore of lakes, we surveyed a lakeshore litter deposit for bacteria growing at $60^{\circ}C$. Ten thermophilic isolates were selected for in-depth characterization, based on their high capacity to degrade high molecular weight organic compounds. Based on phylogenetic analysis on their 16S rRNA gene sequences, all isolates were identified as Geobacillus. The optimal growth temperature and pH of the strains ranged $55{\sim}60^{\circ}C$ and 6.0${\sim}$8.0, respectively. Salinity was inhibitory to the growth of the isolates, showing marked decrease of growth rates at 3% salinity. Based on activities of hydrolytic enzymes and profiles of carbohydrate utilization (determined by API 50 CHB kit), three G. stearothermophilus strains showed patterns clearly distinctive from other isolates. Two G. kaustophilus strains also demonstrated distinctiveness in their metabolic pattern and ecological parameters. However, ecological and metabolic profiles of the other five isolates were more variable and showed some degree of digression from their phylogenetic classification. Therefore, it could be concluded that endospore-forming thermophilic bacteria in lakeshore litter deposits contribute to degradation of organic materials with diverse ecological niches while having successions similar to microbial flora in compost. We propose that the thermophilic isolates and/or their thermo-tolerant enzymes can be applied to industrial processes as appropriate mixtures.
Heading behaviour of native japonica (GI) and Japanese varieties (GII) grown during 1910-1920s were thermo-sensitive, home bred varieties during 1930s (GIII) - 1950s (GIV) were photo-sensitive and semi-dwarf indica derived varieties released since 1971 (GV) were controlled by basic vegetative phase. Number of leaves on main culm was increased with varietal improvement and culm length was gradually shortened as variety improved. GV variety had the shortest plant height among the groups. The first internode length was about 30cm in all groups and difference of 2nd-4th internode length was caused shortening the culm length. Panicle length and diameter of panicle neck were shortened and became thinner as variety developed from GI to GIV, however, GV had longer panicle and thick panicle neck, respectively. Number of panicles/hill and straw weight/hill increased and became heavier as variety improve from GI to GIV but GV had moderate number of panicle/hill and relatively light straw weight. Number of spikelet/panicle has been reduced with varietal improvement but GV had the biggest number. Grain yield/hill also increased as variety improved, however, it was decreased as transplanting season postponed and GV had shown the most sensitive difference. Top dry matter weight at heading stage and at 20 days after heading (20 DAH) had no difference among the groups except GI which was the smallest. Average grain weight at 20 DAH was heavier in the order of GI > GII, GV > GIV > GIII. It had a little variation in GI & GII while that of other variety group showed significant decrease as transplanting delayed. Harvest index (HI) of GV was the highest at 54% for the earlier transplanting and GIII had the lowest HI. Difference of HI became conspicuous for the later transplanting, the varieties bred later had the lower HI when transplanting of rice delayed. At the earliest transplanting applied (May 15), the grain yield was higher in the order of GI > GIV > GIII > GII > GI, however, that of Tongil type variety (GV) was the lowest for the latest transplanting (June 29). Interrelationship among the agronomical characteristics and grain yield was discussed.
Lithium secondary batteries have been widely used in the portable electric devices as power source. Recently it is expected that the realm of its applications expands to the markets such as energy storage medium of hybrid electric vehicle(HEV), electric vehicle(EV). Cathode active material is crucial in terms of performance, durability, capacity of lithium secondary batteries. It is urgent to develope the technology for mass production of cathode material to cope with the markets' demands in the near future. In this study, a calcination furnace running in real production line is modelled in 3D, and the thermal flow and gas flow after chemical reaction in the furnace is analyzed through numerical computations. Based on the results, it is shown that large volume of $CO_2$ gas is generated from chemical reaction. High concentration of $CO_2$ gas and it's stagnation is clearly found from the reactant containers in which the reaction occur to the bottom area of the furnace. It is also studied that 15% or more $CO_2$ mol fraction could affect to proper formation of $LiCoO_2$ through TGA-DSC analysis. The solutions to evacuate carbon dioxide from the furnace are suggested through the change of furnace design and operating condition as well.
Journal of Korean Society of Environmental Engineers
/
v.30
no.3
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pp.331-338
/
2008
Coal-tar enamel, blown asphalt and polyethylene have been used as wrapping materials of steel pipe in Korea. Currently, every manufacturer produces wrapped steel pipes with different materials and methods, and little research has been performed to get on wrapping methods and materials. In this research, properties of wrapping material of steel pipe used for water supply have been evaluated. All of the materials tested in this work were found to meet the standard. Among the wrapping materials of steel pipe tested, blown asphalt and coal-tar enamel were reasonable in price, and their mechanical properties were excellent. The quality of the wrapped steel pipes was being melted easily in organic solvent. When coated thick, the load of the steel pipes was higher than necessary. Tensile strength of cathode exfoliation and PE 3-layer wrapping method was excellent. The pulling intensity of T-Die PE 3-layer was stronger than PE fluidized in PE wrapping method. Cathode exfoliation area was smaller than PE fluidized. Mechanical property and thermo-property of T-Die PE 3-layer were excellent and its anti-chemical property was great. Liquid epoxy can change the property of coating materials depending on the hardening condition and resin selection. Polyurethane used in this test showed a less adhesive strength with steel pipes than epoxy. Moisture absorbance rate was higher than Epoxy's, however. To utilize polyurethane as wrapping materials, basic property of the matter should be improved followed by finding the best suited coating condition. The method of PE 3-layer by extrude method appeared to be the best in this study. However, identification of other wrapping materials requires further additional tests.
Hydrogen is a clean and efficient energy source and is expected to take an important role in future energy demand. A possibly good route to produce hydrogen is by using biomass and organic wastes as a source through thermo-chemical conversion technology. In this study, pyrolysis of wood Pellet(Oregon pine) has been carried out in batch type fixed-bed reactor in $N_2$ atmosphere during 20 minutes to determine the optimum hydrogen generating conditions. At the influence of temperature, hydrogen yield was increased with increasing temperature. For the influence of Steam/Biomass Ratio(SBR), hydrogen yield was increased by steam addition at low temperature condition. However, effect of steam addition was insignificant over at SBR = 1. The hydrogen yield was increased with increasing SBR at high temperature condition. From result of $H_2$/CO and $H_2/CH_4$ ratio, dominant reaction was steam reforming in this experimental condition. The optimum condition for hydrogen production was determined as follows: $H_2$ yield = 38.3 vol.% (56.01 L/min kg) at $900^{\circ}C$, SBR=3.
A gene coding for xylanase from thermo-tolerant Bacillus pumilus TX703 was cloned into Escherichia coli DH5 $\alpha$ using pUC19. Among 7,400 transformants, four transformants showed clear zones on the detection agar plates containing oat-spells xylan. One of them which showed highest xylanase activity was selected and its recombinant plasmid, named pXES106, was found to carry 2.24 kb insert DNA fragment. When the nucleotide sequence of the cloned xylanase gene (xynK) was determined, xynK gene was found to consist of 1,227 base-pair open reading frame coding for a polypeptide of 409 amino acids with a deduced molecular weight of 48 kDa. The coding sequence was preceded by a putative ribosome binding site, the transcription initiation signals, and cia-acting catabolite responsive element. The deduced amino acids sequence of xylanase is similar to those of the xylanases from Hordeum vulgare (barley) and Clostridium thermocellum, with 39 and 31% identical residues, respectively. The amino acids sequence of this xylanase was quite different from those of the xylanases from other Bacillus species.
Ryu, Chang-Ha;Ryu, Dong-Woo;Choi, Byung-Hee;Synn, Dong-Ho;Loui, John P.
Tunnel and Underground Space
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v.18
no.5
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pp.343-354
/
2008
A new method is suggested herein to measure the virgin earth stresses by means of a borehole. This novel concept is basically a combination of borehole stress relieving and borehole fracturing techniques. The destressing of the borehole is achieved by means of inducing thermal tensile stresses at the borehole periphery by using a cryogenic fluid such as Liquid Nitrogen($LN_2$). The borehole wall eventually develops fractures when the induced thermal stresses exceed the existing compressive stresses at the borehole periphery in addition to the tensile strength of the rock. The above concept is theoretically analyzed for its potential applicability to interpret in situ stress levels from the tensile fracture stresses and the corresponding borehole wall temperatures. Coupled thermo-mechanical numerical simulations are also conducted using FLAC3D, with thermal option, to check the validity of the proposed techniques. From the preliminary theoretical and numerical analysis, the method suggested for the measurement of in situ stresses appears to be capable of accurate estimation of the virgin stresses by monitoring tensile crack formation at a borehole wall and recording the wall temperatures at the time of crack initiation.
Kim, Gyeon-Cheol;Kim, Yi-Soon;Kim, Kyu-Kon;Moon, In-Hyuk;Hwang, Lee-Cheol;Gwon, Ja-Youn;Shin, Soon-Shik
Journal of Physiology & Pathology in Korean Medicine
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v.20
no.3
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pp.527-534
/
2006
This is a pilot study to survey the general demand of senior-assistive necessities before a standardization system for senior-assistive necessities is developed as well as to describe health professionals' opinions about the demand and quality of Oriental medical aids and necessities for daily living for the elderly. This is a descriptive survey in which 29 health professionals are questioned, using structured questionnaires based on ISO 9999. The questionnaires were developed by 7 expert conduction standardization system of senior-assistive products in Korea. The data is analyzed by descriptive statistics. The result is as follows : First, with regard to the demand for all of the items in Oriental medical aids for the elderly, the demand of cupping glasses is the highest, followed by instrument used to apply heat treatment, massage equipment, thermo-therapeutic mattress, and heat or ice packs. With regard to the demand for all of the items for the necessities for daily living for the elderly, chairs are the highest, followed by rolling chairs, beds for health, and heigh adjustable beds. Second, with regard to quality of Oriental medical aids, ${\ulcorner}$aids for hair care${\lrcorner}$ are the best, whereas ${\ulcorner}$aids for boiling Oriental medicine${\lrcorner}$ are the worst. In quality of the necessities for daily living, ${\ulcorner}$chairs${\lrcorner}$ are the best, whereas ${\ulcorner}$beds${\lrcorner}$ are the worst. Above all, this result shows that with ${\ulcorner}$aids for heat or cold treatment${\lrcorner}$, there is relatively high demand and low quaily of Oriental medical aids, and with ${\ulcorner}$Beds${\lrcorner}$, there is relatively high demand and low quality. Therefore, aids for heat or cold treatment and beds in th necessities for daily living are required to be developed for standardization of senior-assistive necessities.
Charcoal burning in the process of manufacture and ordinary use often release many constituent chemical species. As a result of open burning, the chemical composition as well as the physical properties of original material changes through the modification of surface properties of charcoal. Surface modification could be more responsible toward the outside elements for surface adsorption, it becomes easy to adsorb more toxic elements through surface adsorption. In this study, four kinds of commercially available charcoal were studied against the chemical and thermal stability along with the heavy metals and organic hazardous substances. Thermo gravimetric analysis (TGA) and differential scanning calorimetry, from room temperature to $400^{\circ}C$, were performed to study the weight loss and the changes in the behavior of those substances. According to TGA analysis, about 10% of weight loss was happened before $200^{\circ}C$. It was found that related weight loss of this temperature region may responsible to the gas phase organic matter. Natural charcoal, K1 and C1 show 15% of loss during the reaction heated to $400^{\circ}C$, while the artificial charcoal K2, C2 show the weight loss of about 20% was found. This is consistent with the main organic matter and VOC analysis results shown. Chemical composition based on the x-ray diffraction analysis was carried out. X-ray diffraction analysis reveals the existence of chemical additive in the forms of $Ba(NO_3)_2$, $BaCO_3$, and $NaNO_3$.
Manganese (Mn) catalysts were generated using $CeO_2$ and $ZrO_2$supports synthesized by the supercritical hydrothermal method and two different Mn precursors, aimed at an application for a low-temperature selective catalytic reduction process. Manganese acetate (MA) and manganese nitrate (MA) were used as Mn precursors. Effects of the kind and the concentration of the Mn precursor used for catalyst generation on the NOx removal efficiency were investigated. The characteristics of the generated catalysts were analyzed using $N_2$ adsorption-desorption, thermo-gravimetric analysis, X-ray diffraction, and X-ray photoelectron spectroscopy. De-NOx experiments were carried out to measure NOx removal efficiencies of the catalysts. NOx removal efficiencies of the catalysts generated using MA were superior to those of the catalysts generated using MN at every temperature tested. Analyses of the catalyst characteristics indicated that the higher NOx removal efficiencies of the MA-derived catalysts stemmed from the higher oxygen mobility and the stronger interaction with support material of $Mn_2O_3$ produced from MA than those of $MnO_2$ produced from MN.
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