• 대한본초학회지
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• 제33권4호
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• pp.35-41
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• 2018

Objectives : Hyeonggaeyeongyotang Gagambang (HYT) is a herbal medicine prescribed for the treatment of inflammatory diseases, but it is necessary to study the exact therapeutic efficacy. This study aims to investigate the antibacterial and anti-inflmmatory activities of HYT. Methods : Antibacterial activity of HYT was confirmed by staining Escherichia coli, a gram negative strain, and Staphylococcus aureus, a gram positive strain, on solid Lysogeny Broth (LB) medium containing HYT. Antioxidant activity of HYT was confirmed by 2,2'-azino-bis-3-ethylbenzothiazoline-6-sulphonic acid (ABTS) assay. The phosphorylation of nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha ($I{\kappa}B{\alpha}$) after lipopolysaccharide (LPS) treatment with HYT-treated RAW 264.7 mouse macrophages cells was confirmed by immunoblot analysis and the level of interleukin 1 beta (IL-$1{\beta}$) mRNA expression level was confirmed by quantitative real-time PCR. Results : HYT showed a concentration-dependent antibacterial activity against Escherichia coli and Staphylococcus aureus and also showed excellent antioxidant activity. HYT treatment attenuated the phosphorylation of $I{\kappa}B{\alpha}$induced by LPS treatment in RAW 264.7 mouse macrophages cells. The phosphorylation of $I{\kappa}B{\alpha}$is crucial for the regulation of the expression of various pro-inflammatory cytokines. In addition, IL-$1{\beta}$ mRNA expression level of RAW 264.7 mouse macrophages cells stimulated by LPS treatment was also inhibited by HYT treatment. Conclusions : Through experimental demonstration of the antioxidative, antimicrobial and anti-inflammatory effects of HYT, we demonstrated that HYT is a herbal medicine effective for the treatment of inflammatory diseases caused by various bacterial infections.

• 한국임상수의학회지
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• 제11권2호
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• pp.507-516
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• 1994

The purposes of this investigations were survey of the occurred bacterial diseases, development of new animal health drug, guidance to formers on the treatment and control methods of diseases. Some series of investigations have been carried out by microbiological, pathological and serological examinations. The results could be summarized as follows. 1. A total of 953 cases of outbreaked swine diseases have been diagnosed in Clinical pathology laboratories, Bayer Vet Res Institute during 8 years (from 1986 to 1993). The high incidence diseases were colibacillosis, pleuropneumonia, streptococcal infection and pasteurellosis in decreasing order. 2. Pleuropneumonia caused by Actinobacillus pleuropneumoniae was the most important respiratory diseases and pasteurellosis by Pasteurella multocide could be confirmed in several cases. 3. Actinobacillus pleuropneumoniae 50 strains were isolated and identified by biochemical and serological tests. In serotyping test, 22 isolated strains were serotype 5, 21 strains as serotype 2, each 2 strains as serotype 3 and 7 by the coagglutination test. 4. Colibacillosis and edema discase caused by Escherichia coli has been the most predominant outbreaked disease in this investigations. The 100 isolates of E coli strains were sensitive to amikacin, colistin, enrofloxacin, gentamycin and trimethoprim -sulfamethoxazole. 5. Swine erysipelas caused by Erysipelothrix rhusiopathiae was confirmed 25 cases as acute septicemic forms. Isolates of E rhusiopathiae were highly sensitive to ampicillin, cephalothin, enrofloxcin, penicillin and tetracycline. 6. The 49 cases of hemorrhagic and necrotic enteritis in piglets were observed and 13 strains of Clostridium perfringens could be isolated and confirmed by biological and serological test. Isolates of Clostridium perfringens type C were highly sensitive to ampicillin, cephalothin, enrofloxacin, penicillin and trimethoprim- sulfamethoxazole. 7. The 14 strains of Streptococcus suis type II could be isolated from meningitis of piplets. 8. Polyserositis caused by Haemophilus parasuis and salmonellosis were observed and confirmed. Also Corynebacterial infections and several parasitosis have been also observed in this investigations.

• 융합정보논문지
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• 제12권1호
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• pp.119-126
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• 2022

본 연구에서는 최적의 조건에서 추출된 지누아리 추출물의 항산화능과 항염능을 확인하였다. 다양한 ethanol 수용액으로 지누아리의 유용물질을 추출하여 DPPH, ABTS, nitrite 라디컬 소거능을 측정하고, 폴리페놀 및 플라보노이드 함량을 분석하였다. 20% ethanol 수용액으로 추출한 경우에 라디컬 소거능이 가장 우수하였고, 폴리페놀과 플라보노이드의 함량 또한 가장 높았다. 이로부터 20% ethanol 수용액이 지누아리의 항산화 및 항염 물질을 효율적으로 추출하였음을 확인하였다. 한편, RAW 264.7 세포주를 이용하여 지누아리 추출물의 세포독성과 항염능을 확인하였다. 지누아리 추출물은 200 ㎍/mL의 농도에서 독성을 나타내지 않았고, 이 농도에서 50% 이상의 NO 생성 억제능을 나타냈다. 이러한 결과로 지누아리 추출물은 항산화 및 항염 특성을 지니는 기능성 화장품 원료로 사용 가능함을 확인하였다.

• Journal of Microbiology and Biotechnology
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• 제22권11호
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• pp.1549-1556
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• 2012

Endophytic fungi were isolated from roots of six halophytes in Suncheon Bay. The endophytic fungi of 35 species isolated from halophytes were identified by internal transcribed spacer (ITS) containing the ITS1, 5.8s, and ITS2 regions. All fungal strains were analyzed to diversity at the genus level. Fungal culture filtrates (FCF) of endophytic fungi were treated to Waito-c rice (WR) seedling for plant growth-promoting verification. It was confirmed that fungal strain Sj-2-2 provided plant growth promotion (PGP) to WR seedling. Then, PGP of Suaeda japonica was confirmed by treating culture filtrate of Sj-2-2. As a result, it was verified that culture filtrate of Sj-2-2 had more advanced PGP than positive control when treated to S. japonica. The secondary metabolites involved in culture filtrate of Sj-2-2 were identified by HPLC and GC-MS SIM analysis. The presence of physiologically bioactive gibberellins (GAs) and other inactive GAs in culture filtrate of Sj-2-2 was detected. The molecular analysis of sequences of Sj-2-2 showed the similarity to Penicillium sp. of 99% homology. The PGP of Sj-2-2 as well as symbiosis between endophytic fungi and halophytes growing naturally in salt marsh was confirmed. Sj-2-2 was identified as a new fungal strain producing GAs by molecular analysis of sequences. Consequently, the Sj-2-2 fungal strain was named as Penicillium sp. Sj-2-2. In this study, the diversity of endophytic fungi isolated from roots of halophytes in salt marsh and the PGP of a new gibberellin-producing fungal strain were confirmed.

• Clinical and Experimental Pediatrics
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• 제61권9호
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• pp.291-300
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• 2018

Purpose: Understanding changes in pathogen and pneumonia prevalence among pediatric pneumonia patients is important for the prevention of infectious diseases. Methods: We retrospectively analyzed data of children younger than 18 years diagnosed with pneumonia at 117 Emergency Departments in Korea between 2007 and 2014. Results: Over the study period, 329,380 pediatric cases of pneumonia were identified. The most frequent age group was 1-3 years old (48.6%) and the next was less than 12 months of age (17.4%). Based on International Classification of Diseases, 10th revision diagnostic codes, confirmed cases of viral pneumonia comprised 8.4% of all cases, pneumonia due to Mycoplasma pneumoniae comprised 3.8% and confirmed cases of bacterial pneumonia 1.3%. The prevalence of confirmed bacterial pneumonia decreased from 3.07% in 2007 and 4.01% in 2008 to 0.65% in 2014. The yearly rate of pneumococcal pneumonia also decreased from 0.47% in 2007 to 0.08% in 2014. A periodic prevalence of M. pneumoniae pneumonia (MP) was identified. Conclusion: The increased number of patients with pneumonia, bacterial pneumonia, pleural effusion, and empyema in 2011 and 2013-2014 resulted from an MP epidemic. We provide evidence that the frequency of confirmed cases of bacterial pneumonia and pneumococcal pneumonia has declined from 2007 to 2014, which can simultaneously reflect the effectiveness of the pneumococcal conjugate vaccine.

• 대한본초학회지
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• 제36권3호
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• pp.39-46
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• 2021

Objectives : Mangifera indica leaves are well known for having a variety of benefits, including anti-inflammatory, anti-tumor, diabetic retinopathy and diabetic vasculosis. However, the effects of Mangifera indica leaves on hair loss inhibition have not been studied. In this study, we investigated to find out the activity of Mangifera indica leaves on hair loss. Methods : 2,2'-azino-bis-3-ethylbenzothiazoline-6-sulphonic acid(ABTS) analysis was performed to confirm the antioxidant efficacy of the water extract of Mangifera indica leaves (WEML). To examine the effect of WEML on cell viability in dermal papillar (DP) cells, 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetra Zolium (MTS) analysis was performed. The changes in the mRNA expression level of the hair loss and hair growth-related genes in dermal papilla cells by WEML treatment were confirmed by quantitative RT-PCR. Results : In dermal papilla (DP) cells, ABTS analysis and MTS analysis of WEML showed antioxidant efficacy and low cytotoxicity. As a result of gene expression analysis through Quantitative RT-PCR, no changes in hair growth-related genes BMP6 and CTNNB1 was confirmed. but inhibitory activity of WEML on hair loss-related genes EGR1, SGK, DKK1, SRD5A1 and SRD5A2 was confirmed. Conclusion : We confirmed that WEML has excellent antioxidant efficacy and a inhibitory activity of hair loss-related genes including 5α-reductase genes. These results suggest that Mangifera indica leaves have a potential activity as a hair loss treatment for hair loss and hair growth. Biochemical or molecular biological research on hair loss is needed.

• 한국정보통신학회논문지
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• 제25권12호
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• pp.1756-1761
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• 2021

2019년 12월경 후베이 우한시에서 발생한 코로나19 바이러스가 점차 줄어드는 듯 보였으나, 2020년 11월, 2021년 6월 기준으로 점차 늘어나고 있으며, 전세계적으로 총 1억 9천 2백만명, 대한민국 기준 총 확진자는 대략 18만4천명으로 추정된다. 이에 따른 대책으로 중앙재난안전대책본부는 사회적 거리두기 4단계를 시행하면서 강력한 대응책을 내고있지만, 델타바이러스등 전염성이 강한 코로나 변이 바이러스가 기승을 부리면서 국내 일일 확진자 수는 1800명대 까지 증가하게 되었다. 그에따라 코로나바이러스의 심각성을 강조하고자 코로나 누적 확진자 수를 ARIMA 알고리즘을 이용해 예측한다. 그 과정에서 추세와 계절성을 제거하기 위해서 차분을 이용하고, MA, AR, 자기상관함수와 편자기상관함수를 이용해 ARIMA에서 p,d,q값을 결정하고 예측한다. 마지막으로 예측값과 실제값을 비교해 얼마나 잘 예측되었는지 평가한다.

• Human Ecology Research
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• 제57권2호
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• pp.225-241
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• 2019

This study develops and validates an objective scale to measure newspaper use at home by elementary school students and parents. We developed a preliminary scale of 59 items through the review of literature on newspaper use and mediation as well as the examination of content validity by education experts. Collected data were analyzed using SPSS 21.0 and AMOS 21.0 programs. A total of 42 items were supported by 703 parents with students in grades 1-6 using exploratory factor analysis. The model included 3 categories and 9 sub-factors: instruction (modeling, restriction, and text instruction), activities (play activity, conversation, online mediation, and scrap activity), and belief (academic achievement and information acquisition). Confirmatory factor analysis confirmed and validated the model fit; in addition, convergent validity, and discriminant validity, and cross validity was confirmed through correlation analysis by gender comparison and grade comparison. We also verified the validity of this scale through correlation analysis based on Yu and Jung (2012)'s newspaper mediation variables and scale in regards to children's motivation for using newspapers. Finally, internal consistency reliability and half reliability were also confirmed. In conclusion, the suitability and stability of home newspaper utilization scale for elementary students (HNUS-E) were confirmed. This scale provides parents and educators with ideas for the development of the children's literacy, cognitive, and affective domains that can be effectively used in research on newspaper use for school-aged children.

• 대한족부족관절학회지
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• 제8권1호
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• pp.1-6
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• 2004

Purpose: We studied the diagnostic value of ultrasonography to confirm the presence of the syndesmosis injuries in the ankle. Materials and Methods: Pre-operative ultrasonography were taken in 42 cases of ankle fractures to determine injury of syndesmosis between February 2000 and August 2003. Tear of syndesmosis was confirmed when they met the following criteria; widening of distal tibiofibular clear space greater than 7 mm on pre-operative AP X-rays, leakage of contrast dye during intra-operative arthrography, and operative findings revealing syndesmosis rupture. We compared these diagnoses with those made by pre-operative ultrasonography. Results: Syndesmosis injuries were confirmed in 23 cases among the total 42 cases. Ultrasonography revealed syndesmosis tear in 25 cases, with 20 cases corresponding with confirmed tear and 5 cases without tear in confirm. Syndesmoses were found to be intact in 17 cases of ultrasonograph, but 3 cases were actually confirmed tear. In ankle ultrasonography, the sensitivity was 90.9%, the specificity was 75.0%. Conclusion: Preoperative ultrasonography is a non-invasive and useful study to determine the syndesmosis injury in ankle fractures.

• 융합정보논문지
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• 제12권4호
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• pp.231-238
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• 2022

본 연구는 대학생의 전공만족이 진로결정 자기효능감과 취업자신감에 미치는 영향을 규명하고자 한다. 연구방법은 서울, 경기도의 뷰티전공 2년제 대학생 335명을 대상으로 설문조사하여 분석에 사용하였다. 수집된 자료는 SPSS 26.0을 이용하여 빈도분석, 요인분석, 신뢰도분석, 전공만족이 진로결정 자기효능감, 취업자신감의 상관관계를 분석하였고, 각 변인들 간에 미치는 영향을 확인하기 위해 다중회귀분석을 실시하였다. 연구의 결과는 첫째, 대학생의 전공만족이 진로결정 자기효능감과 취업자신감에 통계적 유의한 정적(+)상관관계가 확인되었다. 둘째, 전공만족이 자기 효능감에 미치는 영향으로 자기평가, 문제해결, 목표선택에서 통계적 유의미한 영향이 확인되었다. 섯째, 전공만족이 취업자신감에 통계적 유의미한 영향이 확인되었다. 넷째, 진로결정 자기효능감이 취업자신감에서 통계적 유의미한 영향이 확인되었다.