대한본초학회지 (The Korea Journal of Herbology)

  • 제33권4호
  • /
  • Pages.35-41
  • /
  • 2018
  • /
  • 1229-1765(pISSN)
  • /
  • 2288-7199(eISSN)
대한본초학회 (The Korea Association of Herbology)
권호 표지
DOI QR코드

DOI QR Code

형개련교탕(荊芥連翹湯) 가감방(加減方)의 항균 및 항염증 효능

Investigation of Antimicrobial and Anti-inflammatory Activities of the Hyeonggaeyeongyotang Gagambang

  • Gang, Seong Gu (Department of Biochemistry, Chungnam National University) ;
  • Cho, Nam Joon (Department of Biochemistry, Chungnam National University) ;
  • Kim, Ji Young (Department of Biochemistry, Chungnam National University) ;
  • Han, Hyo Sang (Department of Health Administration, Joongbu University) ;
  • Kim, Kee Kwang (Department of Biochemistry, Chungnam National University)
  • 투고 : 2018.04.21
  • 심사 : 2018.07.25
  • 발행 : 2018.07.30
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

Objectives : Hyeonggaeyeongyotang Gagambang (HYT) is a herbal medicine prescribed for the treatment of inflammatory diseases, but it is necessary to study the exact therapeutic efficacy. This study aims to investigate the antibacterial and anti-inflmmatory activities of HYT. Methods : Antibacterial activity of HYT was confirmed by staining Escherichia coli, a gram negative strain, and Staphylococcus aureus, a gram positive strain, on solid Lysogeny Broth (LB) medium containing HYT. Antioxidant activity of HYT was confirmed by 2,2'-azino-bis-3-ethylbenzothiazoline-6-sulphonic acid (ABTS) assay. The phosphorylation of nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha ($I{\kappa}B{\alpha}$) after lipopolysaccharide (LPS) treatment with HYT-treated RAW 264.7 mouse macrophages cells was confirmed by immunoblot analysis and the level of interleukin 1 beta (IL-$1{\beta}$) mRNA expression level was confirmed by quantitative real-time PCR. Results : HYT showed a concentration-dependent antibacterial activity against Escherichia coli and Staphylococcus aureus and also showed excellent antioxidant activity. HYT treatment attenuated the phosphorylation of $I{\kappa}B{\alpha}$induced by LPS treatment in RAW 264.7 mouse macrophages cells. The phosphorylation of $I{\kappa}B{\alpha}$is crucial for the regulation of the expression of various pro-inflammatory cytokines. In addition, IL-$1{\beta}$ mRNA expression level of RAW 264.7 mouse macrophages cells stimulated by LPS treatment was also inhibited by HYT treatment. Conclusions : Through experimental demonstration of the antioxidative, antimicrobial and anti-inflammatory effects of HYT, we demonstrated that HYT is a herbal medicine effective for the treatment of inflammatory diseases caused by various bacterial infections.

키워드

참고문헌

  1. Aderem A, Ulevitch RJ. Toll-like receptors in the induction of the innate immune response. Nature. 2000 ; 406(6797) : 782-7. https://doi.org/10.1038/35021228
  2. Kim DI. Bangyaghabpyeon. Seoul : Ilwolseogag. 1991: 172.
  3. Kong JH Jeungbomanbyeonghoechun hagwon. Seoul : Iljungsa 1994 : 12-4.
  4. Jang YA, Lee JT. The Evaluation of Antioxidant Antiinflammatory and Anti-aging of Extract Solvent and Poria cocos by Parts. Kor. J. Aesthet. Cosmetol. 2015 ; 13(3) : 377-83.
  5. Park EJ, Shin SY. Effects of Hyunggyeyungyotang and Kamihyunggyeyungyotang administration on the anti-inflammation, analgesia and anti-allergic reaction in mice. J Korean oriental pediatrics. 1997 ; 11(1) : 249-73.
  6. Kim MJ, Lee JR, Kim SC, Jee SY. Inhibitory Effect of Hyeonggaeyeongyo-tang Water Extract on production of Nitric Oxide, IL-6 and Expression of iNOS, COX-2 in LPS- Activated Raw 264.7 Cells. Korean J. physiology & pathology. 2007 ; 21(2) : 491-7.
  7. Kim EH, Hwang SY, Kim SC, Jee SY. The Effect of Hyeonggaeyeongyotang Extract on the Fertility, Early Embryonic Development in Wistar rats by Oral Gavage Administration. Korea J. Orental Medical Prescription. 2008 ; 16(1) : 65-78.
  8. Hwang SY, Lee JR, Kim SC, Jee SY. A study on Genotoxicity Test of Hyeong-gae-yeon-gyo-tang extract. Korea J. Herbology. 2007 ; 22(4) : 287-300.
  9. Kang RY, Park BK, Gim SB, Choi HJ, Kim DH. The effects of HYT on various immunological factors related to pathogenesis of allergic dermatitis in NC/Nga mice induced by Biostir AD. Daejeon University J. Korean Oriental Medicine Institute. 2009 ; 18(2) : 47-62.
  10. Kang RY, Park BK, Gim SB, Choi HJ, Kim DH. The effects of HYGB on various immunological factors related to pathogenesis of allergic dermatitis in NC/Nga mice. Daejeon University J. Korean Oriental Medicine Institute. 2009 ; 18(2) : 63-79.
  11. Park JH, Kim JC, Hong SU. The effects of Hyunggaeyungyo-tang of suppression of iNOS production on RAW 264.7cell. J. Korean Oriental Medical Ophthalmology & Otolaryngology & Dermatology. 2011 ; 24(1) : 78-85.
  12. Park JH, Hong SU. The Effects of Hyunggaeyungyo-tang of Suppression of iNOS Production on Mice with Allergic Rhinitis. J. Korean Oriental Medical Ophthalmology & Otolaryngology & Dermatology. 2012 ; 25(1) : 12-21.
  13. Jagdeo J, Adams L, Lev-Tov H, Sieminska J, Michl J, Brody N. Dose-dependent antioxidant function of resveratrol demonstrated via modulation of reactive oxygen species in normal human skin fibroblasts in vitro. J Drugs Dermatol. 2010 ; 9(12):1523-6.
  14. Yin MJ1, Yamamoto Y, Gaynor RB. The antiinflammatory agents aspirin and salicylate inhibit the activity of I(kappa)B kinase-beta. Nature. 1998 ; 396(6706) : 77-80. https://doi.org/10.1038/23948
  15. Kim IR, Kim HC, Kuk YB, Park SJ, Park YK, Park JH, Seo BI, Seo YB, Shin MK, Lee YJ, LeeYC, Lee JH, Leem KH, Cho SI, Chung JK, Joo YS, Choi HY,. Boncho-Hak. Seoul:Young-Lim Press. 2007 : 157, 163, 207, 216, 345, 393, 447, 496, 583, 629, 634.
  16. Miao EA, Leaf IA, Treuting PM, Mao DP, Dors M, Sarkar A, Warren SE, Wewers MD, Aderem A. Caspase-1-induced pyroptosis is an innate immune effector mechanism against intracellular bacteria. Nat Immunol. 2010 ; 11(12) : 1136-42. https://doi.org/10.1038/ni.1960
  17. Schieber M, Chandel NS. ROS function in redox signaling and oxidative stress. Curr Biol. 2014 ; 24(10) : R453-62. https://doi.org/10.1016/j.cub.2014.03.034