The Korea Journal of Herbology (대한본초학회지)

The Korea Association of Herbology (대한본초학회)
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Investigation on the effect of water extracts of Mangifera indica leaves on the hair loss-related genes in human dermal papilla cells

망고 잎 열수 추출물의 모유두 세포에서 탈모 관련 유전자 발현에 미치는 영향 연구

  • Choi, Youngsoo (Department of Biochemistry, Chungnam National University) ;
  • Kim, Eunmi (Department of Predictive Toxicology, Korea Institute of Toxicology) ;
  • Lee, Seong Hee (Huluwa Agriculture & content) ;
  • Han, Hyosang (Department of Health Administration, Joongbu University) ;
  • Kim, Keekwang (Department of Biochemistry, Chungnam National University)
  • 최영수 (충남대학교 생화학과) ;
  • 김은미 (안전성평가연구소 예측독성연구본부) ;
  • 이성희 (홀루아농업) ;
  • 한효상 (중부대학교 보건행정학과) ;
  • 김기광 (충남대학교 생화학과)
  • Received : 2021.03.22
  • Accepted : 2021.05.25
  • Published : 2021.05.30
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Abstract

Objectives : Mangifera indica leaves are well known for having a variety of benefits, including anti-inflammatory, anti-tumor, diabetic retinopathy and diabetic vasculosis. However, the effects of Mangifera indica leaves on hair loss inhibition have not been studied. In this study, we investigated to find out the activity of Mangifera indica leaves on hair loss. Methods : 2,2'-azino-bis-3-ethylbenzothiazoline-6-sulphonic acid(ABTS) analysis was performed to confirm the antioxidant efficacy of the water extract of Mangifera indica leaves (WEML). To examine the effect of WEML on cell viability in dermal papillar (DP) cells, 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetra Zolium (MTS) analysis was performed. The changes in the mRNA expression level of the hair loss and hair growth-related genes in dermal papilla cells by WEML treatment were confirmed by quantitative RT-PCR. Results : In dermal papilla (DP) cells, ABTS analysis and MTS analysis of WEML showed antioxidant efficacy and low cytotoxicity. As a result of gene expression analysis through Quantitative RT-PCR, no changes in hair growth-related genes BMP6 and CTNNB1 was confirmed. but inhibitory activity of WEML on hair loss-related genes EGR1, SGK, DKK1, SRD5A1 and SRD5A2 was confirmed. Conclusion : We confirmed that WEML has excellent antioxidant efficacy and a inhibitory activity of hair loss-related genes including 5α-reductase genes. These results suggest that Mangifera indica leaves have a potential activity as a hair loss treatment for hair loss and hair growth. Biochemical or molecular biological research on hair loss is needed.

Keywords

References

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