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Assessment of Substantial Equivalence and Environmental Risk for Event Selection of Genetically Modified Chrysanthemum (국화의 GM 이벤트 선발을 위한 실질적 동등성 및 환경위해성 평가)

  • Lim, Jin Hee;Shim, Myung Syun;Oh, Kwan Hun;Seo, Ji Yeon;Lee, Su Young;Park, Sang Kun;Shin, Hak Ki;Cho, Myoung Rae
    • Horticultural Science & Technology
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    • v.32 no.4
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    • pp.517-524
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    • 2014
  • As an assessment of substantial equivalence and environmental risks of branchless chrysanthemum GM lines (LeLS69, LeLS76, LeLS80) developed in Korea, we investigated their degree of branchlessness and developmental characteristics including growth and flowering, as well as the stability of populations of insect pests and soil microbes. The GM lines were compared with a control variety, 'Jinba'. LeLS80 showed the highest level of branchlessness among the three GM lines, whereas abnormal axillary buds developed in lines LeLS76 and LeLS69. There were no significant differences between the growth characteristics of 'Jinba' and the GM lines. The branchlessness LeLS80 was increased during the summer season, suggesting that it is a useful germplasm for developing elite branchless cultivars. These GM lines showed no significant differences in the occurrence of five insect pests including aphids, mites, leaf miners, thrips, and whiteflies. Similarly, for soil microbes including bacteria and actinomycetes, there were no significant differences between 'Jinba' and the GM lines. However, there was a higher occurrence of fungi in the GM lines compared to 'Jinba'. Our findings suggest that 'Jinba' and the GM lines are substantially equivalent, although further monitoring studies are needed to confirm this.

Effects of Diet Food Containing Jerusalem Artichoke's Inulin, Lotus Leaf, and Herb on Weight and Body Fat of Obesity University Students (돼지감자의 이놀린, 연잎, 허브의 다이어트제제가 비만인의 체중 및 체성분에 미치는 영향)

  • Lee, Eun-Hye;Kang, Sang-Mo
    • Journal of Applied Biological Chemistry
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    • v.52 no.1
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    • pp.8-14
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    • 2009
  • This study was conducted to assess the effects of diet food containing Jerusalem artichoke's inulin, lotus leaf, and herb on weight and body fat. Participants in this study were selected based on the following criteria: BMI over $25kg/m^2$, body fat percentage higher than 25%, abdominal obesity level of 0.85 measured by body composition measurement unit (ZEUS 9.9 PLUS, Korea) total 24 female and male university students over 20 of age were assigned to two different groups: control group and diet group and the study was carried out for 30 days. When we measured what the tester's body weight and height, we used an automatic measure machin which is called IMI-1000 from Immanuel company and when we measured what the hip and wist circumference, we used a tape measure. When we measured body mss index (BMI), body Int, body mass, lean mass, waist/hip ratio (WHB), obesity index, we used a ZEUS9.9 PLUS-Korea which is based on bioelectrical impedance analysis, The food intake was checked by means of diet record method to be input into CAN program in order to analyze nutrient intake. Our findings indicated that the diet group, as compared to the control group, lost weight of approximately 2.5 kg and showed statistically significant difference. In addition the level of body Int, muscle, abdominal obesity, obesity, waist and bottom measurement all showed significant decrease after study period. However, there was no big difference in body fat percentage because both body fat level and muscle level dropped together, Putting all these together, diet food in this study containing Jerusalem artichoke's inulin, lotus leaf powder, and herbs powder including nettle, eucalyptus was found to be effective in significant reduction of weight and body fat and obesity-related body indicators. Also, it is considered that this diet food has potential to prevent and improve effectively obesity from abnormal fat accumulation.

Recognition and Satisfaction of National Oral Examination for Workers in Incheon and Daejeon (인천·대전지역 근로자의 직장구강검진 인식 및 만족도)

  • Jang, Hye-Mi;Hwang, Soo-Jeong
    • Journal of dental hygiene science
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    • v.14 no.4
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    • pp.516-524
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    • 2014
  • Workers' oral health is important to guarantee workers' right of health and increase productivity. The aim of this study was to investigate recognition and satisfaction of national oral examination for workers in Incheon and Daejeon. Three hundred seventy-nine workers who were selected by convenience cluster sampling answered the questionnaire about experience, recognition and satisfaction of national health examination and oral examination by self recording type. A total of 76.0 percent of the subjects had national health examination, and 51.7% had national oral examination. A total of 64.9 percent had experienced notice about national health examination from the company, and 28.2% about national oral examination. The subjects had high positive score about intention of treatment, dental scaling, and change of toothbrushing method depending on oral examination, need of national health and oral examination, and usefulness to one's health through national health and oral examination. Office workers, regular workers, and the workers with monthly income more than 2 million won had more notice about national health and oral examination compared with sales and production workers, contract workers and the workers with monthly income less than 2 million won. We suggest institutional management of the notice about national oral examination be needed, especially for economically poor, or contract workers to increase the ratio to have national oral examination.

Pulse wave velocity and ankle brachial index in obese adolescents (비만 청소년에서 맥파 속도와 발목 상완 동맥압 지수에 대한 연구)

  • Kim, Ji Hye;Koo, Hee Sun;Hong, Young Mi
    • Clinical and Experimental Pediatrics
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    • v.50 no.11
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    • pp.1078-1084
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    • 2007
  • Purpose : The prevalence of childhood obesity has doubled over the last 30 years. Obesity-associated sequelae in the vasculature begins in the early stages of life. The purpose of this study was to investigate how pulse wave velocity (PWV) and ankle brachial index (ABI) change with height, weight and body mass index (BMI) in obese adolescents. Methods : Seventy-nine obese adolescents (group 1: $85th{\leq}BMI<95th$ percentile, n=40; group 2 ($BMI{\geq}95th$ percentile, n=39) were included. The control group(group 3) included 99 healthy adolescents. Brachial- ankle (ba) PWV and ABI were estimated with blood pressure from four extremities. Heart rate (HR), and pre-ejection period/ejection time (PEP/ET) were also estimated. BMI was calculated from individual height and weight. Linear regression analysis was performed to evaluate the correlations between BMI and PWV. Results : Blood pressure and baPWV were significantly higher in group 2, compared to either group 1 or group 3. However, there was no significant difference in ABI, HR and PEP/ET between the groups. PWV showed linear correlation with both BMI and body weight. Conclusion : Obesity was associated with higher arterial stiffness in adolescents, which was demonstrated by an increase in PWV. There was no significant correlation between obesity and ABI.

Safety Assessments between Commercial Milk and DHA Fortified Milk of Dairy Cows Fed Feeds Containing Protected Fish Oil Treated with Formaldehyde (시중 일반우유와 포름알데히드로 보호 처리된 어유 첨가 사료를 먹인 DHA 강화우유의 안전성 연구)

  • Chun, Su-Hyun;Nam, Mi-Hyun;Hong, Chung-Oui;Yang, Sung-Yong;Yoo, Jin-Ah;Seo, Dong-Won;Chung, Il-Joong;Lee, Kwong-Won
    • Journal of Food Hygiene and Safety
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    • v.26 no.4
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    • pp.349-354
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    • 2011
  • Our objective in this study is to assess the safety of docosahexaenoic acid (DHA) fortified milk of dairy cows fed feeds containing protected fish oil treated with formaldehyde by analyzing formaldehyde concentration in commercial milk and DHA fortified milk of dairy cows fed formaldehyde treated feed. There are 3 milk samples in this study: Commercial milk (CM), DHA fortified milk for Kid (DHA-K) and DHA fortified milk for Baby (DHA-B). We confirm the fresh quality of these three samples by physicochemical tests. In fat content result, three groups are significantly different at the p < 0.05 by Duncan's multiple range test, but fat content of group DHA-K is about half the level of the other two groups. Protein content of group DHA-K is 1 % higher than other two groups. According to the analysis result of DHA content of DHA fortified milk, DHA content of DHA-B is two-fold higher than DHA-K. Similar pattern was seen in the intake based on age. According to HPLC analysis result of formaldehyde concentration in milk, commercial milk and DHA fortified milk are between 0.013 ppm and 0.057 ppm which is formaldehyde standard level in fresh milk settled in WHO (World Health Organization). Three groups have no significantly differences at the p < 0.05 by Duncan's multiple range test. For this reason, it can be concluded that there is no transition of formaldehyde from dairy cows fed formaldehyde treated feeds to its produced milk. Safety about formaldehyde of DHA fortified milk of dairy cows fed formaldehyde treated feeds is considered similar to commercial milk.

Development of High Meat Quality Using Microsatellite Markers in Berkshire Pigs (Microsatellite Marker를 이용한 육질 우수 버크셔 계통 조성에 관한 연구)

  • Lee, Yong-Hwa;Kwon, Seul-Gi;Park, Da-Hye;Kwon, Eun-Jung;Cho, Eun-Seok;Bang, Woo-Young;Park, Hwa-Chun;Park, Beom-Young;Choi, Jong-Soon;Kim, Chul-Wook
    • Journal of Animal Science and Technology
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    • v.53 no.2
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    • pp.89-97
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    • 2011
  • In this study, the efficiency of microsatellite (MS) markers for pork quality was examined and further, their suitability to domestic pork industry also was verified, by measuring meat quality parameters of Berkshire breeds. A total of 323 pigs of Berkshire breeds were slaughtered and subjected to meat quality evaluation. In addition, the genomic DNAs from blood samples of slaughtered pigs were used for genotyping analysis of 50 MS markers. The results revealed that Berkshire breeds have excellent meat quality, compared with the popular domestic breeds such as Duroc, Yorkshire, and Landrace. Noticeably, the Berkshire breeds exhibited a significant post-mortem pH24hr ($5.88{\pm}0.01$) and fat content ($2.878{\pm}0.06$). Through the linkage analysis between MS markers, 14 MS markers showed significant association with meat quality traits (p<0.05). Maximum significant differences of 0.55 pH24hr value and 2.04% fat content were observed between the highest and lowest allele populations. If these 14 MS markers are applied to the pork quality diagnosis kit, the synergistic effect can be expected in meat quality parameters such as meat color, fat content, pH 24 hr, cooking loss, drip loss and water-holding capacity.

Effects of Supplemental Synbiotics Composed of Anaerobic Bacteria, Yeast and Mold on the Aerobic Stability of Total Mixed Ration for Cattle (혐기성 박테리아, 효모 및 곰팡이로 제조된 synbiotics의 첨가가 축우용 완전혼합사료의 호기적 안전성에 미치는 영향)

  • Lee, Shin-Ja;Shin, Nyeon-Hak;Jung, Ho-Sik;Hyun, Jong-Hwan;Moon, Yea-Hwang;Lee, Sang-Suk;Lee, Sung-Sill
    • Journal of Life Science
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    • v.18 no.10
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    • pp.1426-1434
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    • 2008
  • This study was conducted to investigate the effects of supplementation of synbiotics manufactured with anaerobic bacteria, yeast and mold on preservation of total mixed ration (TMR) by exposing days. Eight treatments were composed of untreated synbiotics(US), bacterial synbiotics (BS), yeasty synbiotics (YS), moldy synbiotics (MS), bacterial and mouldy synbiotics (BMS), yeasty and moldy synbiotics (YMS), bacterial and yeasty synbiotics (BYS), and bacterial, yeasty and moldy synbiotics (BYMS). After 7 days of anaerobic fermentation, fermented-TMRs were exposed to the air during 1, 3, 5, 7, 14 and 21 days. One hundred forty four (8 treatments${\times}$6 days${\times}$3 replications) fermented-TMRs were manufactured by vinyl bag ($43\;cm{\times}58\;cm$). Although no significant differences in the activities of carboxymethylcellulase, xylanase and amylase were observed among treatments, theirs acivities were seemed to increase by treatment of BYS or YMS containing yeast. Total bacterial and mold counts also decreased in the treatments containing yeast. Potential pathogenic bacteria were less detected in BYS and BMYS for E. coli, BMYS and YS for Salmonella, and BMS and BMYS for Shigella than those of the other treatments, MS was, however, contaminated easier than US by pathogenic bacteria. From above results, synbiotics containing facultative anaerobic yeast have effects for preservation of TMR fermented anaerobically. Particularly, BMYS treatment having good results in nutrient contents, dry matter loss and pathogenic bacteria amounts was a resonable synbiotics for preservation of the fermented-TMR.

Selection and Horticultural Characteristics Evalution of High ${\alpha}-Glucosidase$ Inhibitor in Pepper (고추의 ${\alpha}-glucosidase$ 저해제 고 활성 계통 선발 및 특성 평가)

  • Cho, Myeong-Cheoul;Park, Dong-Bok;Yang, Eun-Young;Pae, Do-Ham;Won, Se-Ra;Yu, Wang-Kyun;Rhee, Hae-Ik
    • Journal of Bio-Environment Control
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    • v.16 no.3
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    • pp.233-239
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    • 2007
  • This study was carried out to investigate the distribution of ${\alpha}-glucosidase$ inhibitor (AGI) activity and to evaluate horticultural characteristics of pepper (Capsicum spp.). AGI activities of pepper fruits and leaves were different from 1.0 to 20.5 times and 1.0 to 5.9 times, respectively. Weight, length and width of evaluated pepper fruit were distributed from 0.5 to 56.0 g, 0.8 to 15.4 cm and 0.5 to 6.3 cm per fruit respectively. Stem colors before transplanting varied from green to violet. Length and width of leaf were distributed from 3.1 to 5.0 cm and 2.1 to 3.0 cm. Immature fruit color was almost green and mature fruit color was almost red. In horticultural characteristics of selected pepper lines with high AGI activity, the fruit position was downward position. The immature fruit color was green in all lines except one and the mature fruit color was red in all lines. Fruit weight and fruit length of selected pepper lines with high AGI activity were distributed from 5.9 to 41.1 g and 5.9 to 17.0 cm and leaf width and leaf length were distributed from 5.8 to 29.7 cm and 3.9 to 8.7 cm, respectively. The AGI activities of pepper is widely variable between leaf and fruit. According to this result, it suggested the possibility of developing a new pepper line with high AGI activity.

Microflora and enzyme activity of conventional Meju, and isolation of useful mould (재래식 메주의 미생물군, 효소역가 및 유용 균주의 분리)

  • Choi, Seong Hyun;Lee, Mi Hyun;Lee, Seuk Keun;Oh, Man Jin
    • Korean Journal of Agricultural Science
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    • v.22 no.2
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    • pp.188-196
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    • 1995
  • To obtain useful mould strain in soybean fermentation industry, we collected conventional Meju all over the Korea and tested existance of aflatoxin in Meju collected. Also, we measured distribution of microorganism and enzyme activity of Meju and isolated Aspergillus oryzae O4-5 as a industrially useful mould. The results obtained were summarized as follows: 1. Aflatoxin was not detected by EZ-Screen Test Kit and HPLC analysis in various Meju sample collected all over the Korea from 1994.12 to 1995.2. 2. Colony forming units(CFU) of mould, yeasts, aerobe, and anaerobe were $1.3{\times}10^4-2.8{\times}10^6$, $1{\times}10^2-1.5{\times}0^6$, $2.0{\times}10^7-8.0{\times}10^8$ and $3.0{\times}10^6-7.3{\times}10^8$ per gram of Meju, respectively, indicating that CFU inter Meju samples were varied with big difference. 3. The activities of ${\alpha}$-amylase and gluco-amylase were 5-80 units and 2-34 units per g of Meju, respectively. It was shown that enzyme activity was varied depending on where the Meju was collected. 4. The activities of acidic, neutral and alkaline protease were 5-33 units, 5-302 units and 5-363 units per g of Meju, respectively. Acidic protease of Improved Meju made by D-Company was higher than that of conventional Meju as 66 units per g of Meju. 5. CNU O4-5 strain selected as a noble strain could produce amylase and protease in high level, and identified as a strain that belongs to Aspergillus oryzae. 6. The activities of acidic and neutral protease of Aspergillus oryzae CNU O4-5 strain isolated were about 10% higher than those of Aspergillus oryzae JM which has been used in soybean fermentation industry. Amylase activity of CNU O4-5 strain was similar to Aspergillus oryzae JM.

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Skin diseases of male workers in painting workplace (도장 부서 남성 근로자들의 피부 질환)

  • Park, Jae-Beom;Lee, Kyung-Jong;Jang, Jae-Yeon;Chung, Ho-Keun
    • Journal of Preventive Medicine and Public Health
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    • v.30 no.4 s.59
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    • pp.830-839
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    • 1997
  • Painting is risky work to occupational skin disease. This research was carried out to investigate the prevalence of occupational skin diseases in painting department of a shipyard company in June 1996. Dermatological examination, self-administered questionnaire survey were conducted. Exposed group(n=379) was selected randomly in painting department and control of group(n=151) was selected in those who had not exposed to paints or solvents. The prevalence of contact dermatitis(11.9%) is significantly elevated compared with control of group(2.6%), and odds ratio(OR) is 4.95 (95% confidence interval[CI]: 1.75-14.02). The prevalence of Tinea pedis, the most common skin disease, in exposure group is 48.0%, and its odds ratio(OR) is significantly elevated compared with control group (OR:3.18, 95% CI: 2.06-4.90). Pompholyx is also significantly elevated in prevalence(11.9%) and OR(OR:6.64, 95% CI:2.03-21.69). There were no difference in the prevalence of contact dermatitis, Tinea pedis, and pompholyx by use of personal protective equipments(PPE), job categories, and duration of employment in painting department. In exposure group, 71.1% suffer from contact dermatitis improved in vacation or holiday and 68.9% of them had lesion on exposed area, it suggest that contact dermatitis in exposed group is related to their work. In conclusion, workers in painting department have high risks of contact dermatitis, Tinea pedis, and pompholyx. A health policy should be provided to prevent skin disease among painting department.

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