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http://dx.doi.org/10.5352/JLS.2008.18.10.1426

Effects of Supplemental Synbiotics Composed of Anaerobic Bacteria, Yeast and Mold on the Aerobic Stability of Total Mixed Ration for Cattle  

Lee, Shin-Ja (Division of Applied Life Science(BK21 Program), Graduate School Gyeongsang National University)
Shin, Nyeon-Hak ( Division of Applied Life Science(BK21 Program), Graduate School Gyeongsang National University)
Jung, Ho-Sik (Hanyang Feed Industrial Company limited)
Hyun, Jong-Hwan ( Division of Applied Life Science(BK21 Program), Graduate School Gyeongsang National University)
Moon, Yea-Hwang (Department of Animal Science & Biotechnology, Jinju National University)
Lee, Sang-Suk (Department of Animal Science & Biotechnology, Sunchon National University)
Lee, Sung-Sill (Division of Applied Life Science(BK21 Program), Graduate School Gyeongsang National University)
Publication Information
Journal of Life Science / v.18, no.10, 2008 , pp. 1426-1434 More about this Journal
Abstract
This study was conducted to investigate the effects of supplementation of synbiotics manufactured with anaerobic bacteria, yeast and mold on preservation of total mixed ration (TMR) by exposing days. Eight treatments were composed of untreated synbiotics(US), bacterial synbiotics (BS), yeasty synbiotics (YS), moldy synbiotics (MS), bacterial and mouldy synbiotics (BMS), yeasty and moldy synbiotics (YMS), bacterial and yeasty synbiotics (BYS), and bacterial, yeasty and moldy synbiotics (BYMS). After 7 days of anaerobic fermentation, fermented-TMRs were exposed to the air during 1, 3, 5, 7, 14 and 21 days. One hundred forty four (8 treatments${\times}$6 days${\times}$3 replications) fermented-TMRs were manufactured by vinyl bag ($43\;cm{\times}58\;cm$). Although no significant differences in the activities of carboxymethylcellulase, xylanase and amylase were observed among treatments, theirs acivities were seemed to increase by treatment of BYS or YMS containing yeast. Total bacterial and mold counts also decreased in the treatments containing yeast. Potential pathogenic bacteria were less detected in BYS and BMYS for E. coli, BMYS and YS for Salmonella, and BMS and BMYS for Shigella than those of the other treatments, MS was, however, contaminated easier than US by pathogenic bacteria. From above results, synbiotics containing facultative anaerobic yeast have effects for preservation of TMR fermented anaerobically. Particularly, BMYS treatment having good results in nutrient contents, dry matter loss and pathogenic bacteria amounts was a resonable synbiotics for preservation of the fermented-TMR.
Keywords
Synbiotics; anaerobic fermentation; total mixed ration; preservation;
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1 Ha, J. K., S. S. Lee, Y. S. Moon and C. H. Kim. 2005. Ruminant Nutrition and physiology. pp. 154, Seoul National University press.
2 Hara, S., M. Itoh. and Y. Ohyama. 1979. Aerobic deterioration of silages changes in temperature, gas metabolism, heat production and microflora. Jpn. J. Zootech. Sci. 50, 549-556
3 Keady, T. W. J. and J. J. Murphy. 1996. Effects of inoculant Treatment on ryegrass silage fermentation, digestibility, rumen fermentation. and performance of lactating dairy cattle. Grass Forge Sci. 51, 232-241.   DOI   ScienceOn
4 Harris, B. Jr., H. H. Van Horn, K. E. Manookian, S. P. Marshal, M. J. Taylor, and C. J. Wilcox. 1983. Sugarcane silage, sodium hydroxide and steam pressure-treated sugarcane bagasse, corn silage, cottonseed hulls, sodium bicarbonate, and Aspergillus oryzae product in complete rations for lactating cows. J. Dairy Sci. 66, 1474-1485.   DOI
5 Holdman, L. V., E. P. Coto and W. E. C. Moore. 1977. Anaerobic laboratory manual (4th eds), Virginia Polytech. Inct. and State Univ. Blackburg, Virginia, USA.
6 Ko, Y. D. 1999. Silage manufacturing strategy of Rumen Animal. Sun Jin. pp30-31.
7 Kim, J. W., J. S. Park. 1997. Industrial effective microorganisms(III). Classification, Characterization and Industrial use of Yeast. Kor. Jour. of Microbiololgy and Biotechnology. 10, 26-33.
8 Kelly, D. 1998. Probiotics in young and newborn animals. J. Anim. Feed Sci. 7, 15-23.   DOI
9 Kellems, R. O., A. Lagerstedt and M. V. Wallentine. 1990. Effect of feeding Aspergillus oryzae fermentation extract or Aspergillus oryzae plus yeast culture plus mineral and vita min supplement on performance of Holstein cows during a complete lactation. J. Dairy Sci. 73, 2922-2928.   DOI   ScienceOn
10 Gibson, G. R. and B. M. Roberfroid. 1995. Dietary modulation of the human colonic microbiota: introducing the concept of prebiotics. J. Nutr. 125, 1401-1412.
11 EI Hassan, S. M., C. J. Newbold, I. E. Edwards, J. H. Topps and J. R. Wassace. 1996. Effect of yeast culture on rumen fermentation, microbial protein flow from the rumen and live-weight gain in bulls given high-cereal diets. J. Anim. Sci. 62, 43-48.   DOI
12 Duncan, D. B. 1955. Multiple range and multiple F tests. Biometrics. 11, 1-42.   DOI   ScienceOn
13 Han, S. W., Lee, K. W., Lee, B. D. and C. G. Sung. 1999. Effect of feeding Aspergillus oryzae culture on fecal microflora, egg qualities, and nutrient metabolizabilities in laying hens. Asian-Aus. J. Anim. Sci. 12, 417-421.   DOI   ScienceOn
14 Driehuis, F., S. J. W. H. Oude EIferink and S. F. Spoelstra. 1999a. Anaerobic lactic acid degradation during ensilage of whole crop maize inoculated with Lactobacillus buchneri inhibits yeast growth and improves aerobic stability. J. Appl. Microbiol. 87, 583-594.   DOI   ScienceOn
15 De Man, J. C., M. Rogosa and M. E Sharpe. 1960. A medium for the cultivation of lactobacilli. J. Appl. Bact. 23, 130-135.   DOI
16 Driehuis, F., S. J. W. H. Oude EIferink and P. G. Van Wikselaar. 1999b. Lactobacillus buchneri improves aerobic stability of laboratory and farm scale whole crop maize silage but does not affect feed intake and milk production of dairy cows, pp. 106-107. In Proceedings of the 12th International Silage Conferenceed. Pauly, T. Uppsala: Swedish University of Agricultural Sciences.
17 Gomez-Alarcon, R. A., C. Dudas and J. T. Huber. 1990. Influence of cultures of Aspergillus oryzae on rumen and total tract digestibility of dietary components. J. Dairy Sci. 73, 703-710.   DOI   ScienceOn
18 Williams, A. G. and C. G. Orpin. 1987. Polysaccharide-degrading enzymes formed by three species of anaerobic rumen fungi grown on a range of carbohydrate substrates. Can. J. Microbiol. 33, 418-426.   DOI   ScienceOn
19 Woolford, M. K. and J. E. Cook. 1978. A note on the effect on the aerobic deterioration of maize silage on the manipulation of the microflora by means of antibiolotics. Anim. Feed Sci. Technol. 3, 89-94.   DOI   ScienceOn
20 Cussen, R. F., R. J. Merry, A. P. Williams and J. K. S. Tweed. 1995. The effect of additives on the ensilage of forage of differing perennial ryegrass and white clover content. Grass Forage Sci. 50, 249-258.   DOI
21 American Public Health Association. 1992. Standard methods for the examination of dairy products. 16th eds. APHA Inc. Washington DC.
22 Beak, T. and F. Gross. 1964. Ursachen der unterschiedlichen Habarkeit von Gaerfutter. Das Wirtschftseigene Futter. 10, 298-312.
23 Fuller, R. 1989. Probiotics in man and animals. J. Appl. Bact. 66, 365-378.   DOI
24 Weinberg, Z. G. and R. E. Muck. 1996. New trends in development and use of inoculants for silage. FEMS Microbiol. Rev. 19, 53-68.   DOI   ScienceOn
25 Stokes, M. R. 1992. Effects of an enzyme mixture, an inoculant, and their interaction on silage fermentation and dairy production. J. Dairy Sci. 75, 764-773.   DOI   ScienceOn
26 Roper, K. B. and D. I. Fennell. 1965. The Genus Aspergillus. pp 357-404, The Williams and Wilkins Co., Baltimore, MD.
27 Rose, A. H. 1980. Rent research on industrially important strains of Saccharomyces cerevisiae. In Skimmer, F. A., S. M. Passmore and R. R. Danenpor (eds.), Biology and Activities of yeasts. The Society for Applied Bacteriology Symposium Series 9, 103. Academic Press, London. UK.
28 SAS. 1999. SAS/STAT software for PC. Release 8.01. SAS institute Inc., Cary, N. C., U.S.A.
29 Sniffen, C. J. 1986. Natural growth stimulators: Micronutrient requirements of the rumen and the role of the yeast cultures in the rumen. Altech's Second Annual Symposium Lecington, Kentucky, USA.
30 Phillips, M. W. and G. L. R. Gordon. 1988. Suger and polysaccharide fermentation by anaerobic fungi from Australia, Britian and New Zealand. Biosystems 21, 377-383.
31 Ranjit. N. K., C. C. Taylor and L. King. 2002. Effect of Lactobacillus buchneri 40788 on the fermentation, aerobic stability and nutrituve value of maize silage. Grass Forage Sci. 57, 73-81.   DOI   ScienceOn
32 Woolford, M. K. 1990. The detrimental effects of air on silage. J. Appl. Bacteriol. 68, 101-116.   DOI
33 Wiedmeier, R. D., M. J. Arambel and J. L. Walters. 1987. Effect of yeast culture and Aspergillus oryzae fermentation extract in ruminal characteristics and nutrient digestibility. J. Dairy Sci. 70, 2063-2068.   DOI   ScienceOn
34 Nisbet, D. J., D. E. Corrier, C. M. Scanlan, A. G. Hollister, R. C. Beier and J. R. Deloach. 1993. Effect of defined continuous- flow derived bacterial culture and dietary lactose on Salmonella colonization in broiler chicks. Avian Disease 37, 1017-1025.   DOI   ScienceOn
35 Oude Elferink, S. J. W. H., J. Krooneman, J. C. Gottschal, S. F. Spoelstra, F. Faber and F. Driehuis. 2001. Anaerobic aconversion of lactic acid to acetic acid and 1, 2 propanediol by Lactocacillus buchneri. Appl. Environ. Microbiol. 67, 125-132.   DOI   ScienceOn
36 Mountfort, D. O. and R. A. Asher. 1988. Production of $\alpha$- amylase by the ruminal anaerobic fungus Neocallimastix frontalis. Appl. Environ. Microbiol. 54, 2293-2299.
37 Nicholson, J. W. G., R. E. McQueen, E. Chan and R. S. Bush. 1991. Forage conservation round bales or silage bage: effect on the characteristics and performance. Anim. Sci. 71, 1167-1180.
38 Mitsuoka, T. 1980. Zonaisaikinno Sekai. Sobunsha 21, 1-34.
39 Pearce, P. D. and T. Bauchop. 1985. Glycosidases of the rumen anaerobic fungus Neocallimastix frontalis grown on cellulosic substrates. Appl. Environ. Microbiol. 49, 1265-1269.
40 Ok, J. U., S. M. Lee, S. J. Lee, J. H. Lim, T. W. Kang, H. Y. Jung, Y. H. Moon and S. S. Lee. 2006. Effect of Yeast Addition in Rice Straw Silage Fermentation. J. Anim. Sci. & Technol. (Kor.) 48, 691-698.   과학기술학회마을   DOI   ScienceOn
41 Nishino, N., H. Wada, M. Yoshida and H. Shiota. 2004. Microbial counts, fermentation products, and aerobic stability of whole crop corn and a Total Mixed Ration ensiled with and Without inoculation of Lactobacillus casei or Lactocacillus buchneri. J. Dairy Sci. 87, 2563-2570.   DOI   ScienceOn
42 Ogimoto, K. and S. Imai. 1981. Atlas of Rumen Microbiology. Japan. Scientific Societies Press. Tokyo, Japan.
43 Pettersson, D., H. Graham and p. Amen. 1989. Enzyme supplementation of broiler chickens. Ani. Prod. 51, 399-404.
44 Kung, Jr. L. and N. K. Ranjit. 2001. The effect of Lactobacillus buchneri and other additives on the fermentation and aerobic stability of barley silage. J. Dairy Sci. 84, 1149-1155.   DOI   ScienceOn
45 Marcus, K. M., J. T. Huber and S. Cramer. 1986. Influence of feeding Vita during hot weather on performance of lactating cows in a large dairy herd. J. Dairy Sci. 69 (Suppl. 1), 188 (Abstr.).
46 Miller, G. L., R. Blum, W. E. Giennon and A. L. Burton. 1960. Measurement of Carboxymethylcellulase activity. Anal. biochem. 1, 127-132.   DOI
47 Line, J. E., J. S. Bailey, Cox, N. A., N. J. Stem and T. Tompkins. 1998. Effect of yeast supplemented feed on Salmonella and Campylobacter populations in broilers. Poultry Sci. 77, 405-410.   DOI
48 Kumar, U., Sareen, V. K. and S. Singh. 1997. Effect of yeast culture supplement on ruminal microbial populations and metabolism in buffalo calves fed a high roughage diet. J. Sci Food Agric. 73, 231-236.   DOI   ScienceOn