• Journal of Plant Biotechnology
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• 제35권4호
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• pp.329-335
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• 2008

Tocopherols are essential lipophilic antioxidant in human cells, while little is known about its function in plant tissues. To study the impact of composition and content of tocopherols on stress tolerance, tobacco (Nicotiana tabacum) was transformed with a construct containing a cDNA insert encoding $\gamma$-tocopherol methyltransferase ($\gamma$-TMT/VTE4) from perilla under the control of the cauliflower mosaic virus (CaMV) 35S promoter. The transgenic tobacco was confirmed by PCR and RT-PCR. The total content and composition of tocopherols in the transgenic lines were similar with wild type controls. However, chlorophyll-a and carotenoid content in the transgenic lines were increased by up to 45% (P<0.01) and 39% (P<0.02), respectively. Also, the over-expression of $\gamma$-TMT increased the salt stress tolerance in tobacco plants. These results demonstrate that over-expression of $\gamma$-TMT gene in tocopherol bio-synthetic pathway can increase salt stress tolerance and contents of chlorophyll-a and carotenoid in transgenic tobacco plants.

• Pediatric Gastroenterology, Hepatology & Nutrition
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• 제26권5호
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• pp.249-265
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• 2023

Purpose: Infant regurgitation is associated with other functional gastrointestinal disorders and signs and symptoms that have a major impact on the quality of life of infants and their families. This study evaluated the safety, tolerance, and real-world effectiveness of an anti-regurgitation formula containing locust bean gum (LBG), prebiotics, and postbiotics to alleviate digestive symptoms beyond regurgitation. Methods: This 3-month study involved infants with regurgitation requiring the prescription of an anti-regurgitation formula according to usual clinical practice. Outcomes included evaluation of the evolution of stool consistency and frequency; occurrence of colic, constipation, and diarrhea; and assessment of regurgitation severity. Infant crying, parental assessment of infant well-being, and parental satisfaction with the stool consistency were also evaluated. Results: In total, 190 infants (average age: 1.9±1.1 months) were included. After three months, stool frequency and consistency remained within the normal physiological range, with 82.7% of infants passing one or two stools per day and 90.4% passing loose or formed stools. There was no significant increase in the number of infants with diarrhea, whereas a decrease was observed in the number of infants with constipation after 1 month (p=0.001) and with colic after both 1 and 3 months (p<0.001). Regurgitation severity and crying decreased and parental satisfaction with stool consistency, formula acceptability, infant well-being, and sleep quality increased. Monitoring of adverse events did not reveal any safety concerns. Conclusion: Formulas containing LBG, prebiotics, and postbiotics were well tolerated and provided an effective strategy for managing infant regurgitation and gastrointestinal discomfort.

• Journal of Plant Biotechnology
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• 제38권2호
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• pp.117-124
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• 2011

Considering that the world population is expected to total 9 billion by 2050, it will clearly be necessary to sustain and even accelerate the rate of improvement in crop productivity. In the 21st century, we now face another, perhaps more devastating, environmental threat, namely climate change, which could cause irreversible damage to agricultural ecosystem and loss of production potential. Enhancing intrinsic yield, plant abiotic stress tolerance, and pest and pathogen resistance through agricultural biotechnology will be a critical part of feeding, clothing, and providing energy for the human population, and overcoming climate change. Development and commercialization of genetically engineered crops have significantly contributed to increase of crop yield and farmer's income, decrease of environmental impact associated with herbicide and insecticide, and to reduction of greenhouse gas emissions from this cropping area. Advances in plant genomics, proteomics and system biology have offered an unprecedented opportunities to identify genes, pathways and networks that control agricultural important traits. Because such advances will provide further details and complete understanding of interaction of plant systems and environmental variables, biotechnology is likely to be the most prominent part of the next generation of successful agricultural industry. In this article, we review the prospects for modification of agricultural target traits by genetic engineering, including enhancement of photosynthesis, abiotic stress tolerance, and pest and pathogen resistance associated with such opportunities and challenges under climate change.

• 한국작물학회:학술대회논문집
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• 한국작물학회 2018년도 추계학술대회
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• pp.179-179
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• 2018

Potato (Solanum tubersosum L.) is susceptible to various environmental stresses such as salt, high temperature, and drought. Especially, potato tuber growth is greatly affected by drought that causes not only yield reduction but also loss of tuber quality. Since unpredictable global weather changes cause more severe and frequent water limiting conditions, improvement of potato drought tolerance can minimize such adverse effects under drought and can impact on sustainable potato production. Genetic engineering can be utilized to improve potato drought tolerance, but such approaches using endogenous potato genes have rarely been applied. We were obtained AtbZIP28 gene transgenic potato plants. It is identified transcript levels at various stress conditions, polyethylene glycol (PEG), NaCl, (ABA). Also, For identification to regulate ER stress response genes in AtbZIP28 gene transgenic potato plant, we screened seven potato genes from RNA-seq analysis under TM treatment. Five and two genes were up- and down-regulated by TM, respectively. Their expression patterns were re-examined at stress agents known to elicit TM, DTT, DMSO and salt stress.

• 한국작물학회:학술대회논문집
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• 한국작물학회 2017년도 9th Asian Crop Science Association conference
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• pp.153-153
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• 2017

Potato (Solanum tubersosum L.) is susceptible to various environmental stresses such as frost, high temperature, and drought. Enhancement of potato drought tolerance can reduce yield loss under drought that has negative effect on potato tuber growth. Genetic engineering can be utilized to achieve this goal, but such approaches using endogenous potato genes have rarely been applied. Since unpredictable global weather changes cause more severe and frequent water limiting conditions, improvement of potato drought tolerance can minimize such adverse effects under drought and can impact on sustainable potato production. Genetic engineering can be utilized to improve potato drought tolerance, but such approaches using endogenous potato genes have rarely been applied. We were obtained AtbZIP28 gene transgenic potato plants. It is identified transcript levels at various stress conditions, polyethylene glycol (PEG), NaCl, abscisic ${\underline{acid}}$ (ABA). Also, For identification to regulate ER stress response genes in AtbZIP28 gene transgenic potato plant, we screened seven potato genes from RNA-seq analysis under TM treatment. Five and two genes were up- and down-regulated by TM, respectively. Their expression patterns were re-examined at stress agents known to elicit TM, DTT, DMSO and salt stress.

• 통상정보연구
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• 제14권3호
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• pp.113-138
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• 2012

본 연구는 우리나라 중소제조기업의 수출성과 결정요인을 살펴보았다. 우선 기존 연구를 살펴보고, 수출성과 선행요인으로 기업가정신과 제품차별화 역량을 설정하였다. 또한, 수출성과의 선행요인으로서 제품차별화 역량을 기업가정신과 수출성과의 매개요인으로 분석하였다. 이로써, 기업가정신, 제품차별화 역량 그리고 수출성과의 통합적 모형을 구축하고 그 관계를 살펴보는 것을 연구의 목적으로 하였다. 실증분석에서는 국내 중소제조 수출기업 152개 표본에 PLS(Partial Least Square) 분석을 이용하였다. 분석도구는 SmartPLS2.0을 사용하였다. 분석 결과 중소제조기업 기업가의 위험감수성과 혁신성은 제품차별화 역량과 수출성과에 모두 긍정적 영향을 미쳤다. 제품차별화 역량 역시 중소제조 수출기업의 수출성과에 긍정적 영향을 준다는 점을 확인하였다. 매개효과 분석에서는 제품차별화 역량이 기업가정신 중 혁신성과 수출성과 사이에 유의한 매개효과를 나타냈다. 그러나 위험감수성에는 유의한 매개효과를 나타내지 못했다. 이러한 결과는 중소제조 수출기업에 제품차별화 역량이 필요할수록 적극적으로 혁신성향을 추구해야 한다는 점을 시사한다. 즉, 제품차별화를 시도할 때 위험감수성보다 혁신성향을 강조하는 것이 의미가 있음을 보여준다. 이에 본 연구는 제품차별화 역량의 매개효과를 분석한 점에서 동적역량이나 경쟁우위 관점에서 중소제조기업의 수출성과를 살펴보려는 연구에 이론적 시사점을 가진다. 또한, 중소제조 기업 경영자에게는 위험감수성과 혁신성의 역할이 모두 중요하지만, 낯선 환경의 외국시장 경쟁에 필요한 제품차별화 역량에는 혁신성향의 역할에 더 주목해야 한다는 실무적 시사점을 제시한다.

• 환경영향평가
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• 제21권5호
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• pp.631-648
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• 2012

The integrative ecological approaches of chemical assessments, physical habitat modelling, and multi-metric biological health modelling were applied to Gwanpyeong Stream within Gap-Stream watersheds to evaluate environmental impacts on the constructions of residential and commercial complex. For the analysis, the surveys conducted from 45 sites of reference streams within the Gap-Stream watershed and 3 regular sites during 2009 - 2010. Physical habitat health, based on the habitat model of Qualitative Habitat Evaluation Index(QHEI) declined from the headwaters(good - fair condition) to the downstream(poor condition). Chemical water quality, based turbidity and electric conductivity(EC), was degraded toward to the downstream, and especially showed abrupt increases, compared to the values of control streams(CS). Also, concentrations of chlorophyll-a in the downstreams were greater compared to the control stream(CS), indicating an eutrophication. Biological health conditions, based on the Index of Biological Integrity(IBI) using fish assemblages, averaged 19.3 which is judged as a fair condition by the biological criteria of the Ministry of Environment, Korea. The comparisons of model metric values in sensitive species and riffle-benthic species on the Maximum Species Richness Line(MSRL) of 45 reference streams indicated a massive disturbances in all sampling locations. Also, tolerance guild and trophic guild analyses suggest that dominances of tolerant species and omnivores were evident, indicating a biological degradation by habitat disturbances and organic matter pollutions. There was no distinct longitudinal variations of IBI model values from the headwater to the downstream in spite of slight chemical and habitat health gradients among the sampling sites. Overall, integrative ecological health(IEH) scores, based on the chemical, physical, and biological parameters, were low compared to the 45 reference streams due to physical and chemical disturbances of massive constructions of the residential and commercial complex. This stream, thus showed a tendency of typical urban streams which are disturbed in the chemical water quality, habitat structures, and biological integrity. Effective stream management plans and restoration strategies are required in this urban stream for improving integrative stream health.

• 환경영향평가
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• 제21권6호
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• pp.841-857
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• 2012

Preliminary ecological environmental assessments including physico-chemical constituents, water quality, fish fauna analysis, physical habitat health, and ecological health assessment were conducted as a primary step for Jeungchon micro-habitat ecosystem restoration in 2012. Water chemistry analysis of conductivity, dissolved oxygen, chlorophyll-a and etc. indicated that there were no significant differences(p < 0.05) among 6 sites between the headwaters and downstream. Multi-metric model analysis of Qualitative Habitat Evaluation Index(QHEI) showed that brooklets were at "good condition" as a mean QHEI of 158.7(n = 6) and the longitudinal differences of the model values between the sites were minor(QHEI range: 153 - 165). Total fish species and the number of individuals were 12 and 481, respectively, and dominant species were Zacco platypus(49.5%) and Zacco koreanus(36.8%). Tolerance guild analysis showed that the proportion of sensitive species($S_S$) had a negative linear function[$S_S=86.35-0.31(D_H)$; $R^2$ = 0.892, p < 0.01] with a distance from the headwaters, while the proportion of tolerant species($T_S$) had a positive linear function($R^2$ = 0.950, F = 90.28, p < 0.001) with the distance. Trophic feeding guild analysis showed that the proportion of insectivore species($I_n$) had a negative linear function($R^2$ = 0.934, p < 0.01) with a distance from the headwaters, while the proportion of omnivore species($O_m$) had a positive linear function($R^2$ = 0.958, p < 0.001) with the distance. Index of Biological Integrity(IBI) model, based on fish assemblages, showed a "fair condition" as a mean IBI of 23(n = 6), and there was a distinct differences of ecological health between the headwaters(S1 = 30; "good condition") and the downstreams(S6 = 14; "poor condition"). Overall, the preliminary environmental impact assessments suggest that water quality, physical habitat conditions(QHEI model), and ecological health(IBI model) were maintained well, even if the state was not an excellent conditions.

• KSII Transactions on Internet and Information Systems (TIIS)
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• 제10권3호
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• pp.976-995
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• 2016

Workflow scheduling is one of the challenging problems in cloud computing, especially when service reliability is considered. To improve cloud service reliability, fault tolerance techniques such as fault recovery can be employed. Practically, fault recovery has impact on the performance of workflow scheduling. Such impact deserves detailed research. Only few research works on workflow scheduling consider fault recovery and its impact. In this paper, we investigate the problem of workflow scheduling in clouds, considering the probability that cloud resources may fail during execution. We formulate this problem as a multi-objective optimization model. The first optimization objective is to minimize the overall completion time and the second one is to minimize the overall execution cost. Based on the proposed optimization model, we develop a heuristic-based algorithm called Min-min based time and cost tradeoff (MTCT). We perform extensive simulations with four different real world scientific workflows to verify the validity of the proposed model and evaluate the performance of our algorithm. The results show that, as expected, fault recovery has significant impact on the two performance criteria, and the proposed MTCT algorithm is useful for real life workflow scheduling when both of the two optimization objectives are considered.

• 디지털융복합연구
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• 제14권4호
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• pp.239-249
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• 2016

본 연구에서는 청소년들이 가진 마음의 창 유형으로 개방형, 주장형, 신중형, 고립형의 네 기질들로 분류하고 스마트폰 중독에 미치는 영향을 분석하였다, 연구대상은 전북소재 3개 중학생 406명 이었으며, 측정도구로는 Luft(1970)의 '조해리 마음의 창' 검사와 한국정화진흥원이 개발한 '스마트폰 중독 진단척도'를 사용하였다. 본 연구의 결과 첫째, 성별에 관한 분석결과를 살펴보면, 성별에 따라서 청소년 스마트폰 중독의 차이점은 크지 않으나 남학생의 경우 내성에 영향을 받는 경우가 크고, 여학생의 경우 일상생활장애에 영향을 받고 있음을 알 수 있다. 둘째, 학년에 따른 차이에 관한 분석결과를 살펴보면, 스마트폰 중독의 하위요인인 일상생활장애, 내성은 통계적으로 매우 높은 유의미한 차이가 있는 것으로 나타났다. 셋째, 마음의 창 유형과 스마트론 중독간의 분석결과 주장형과 고립형은 스마트혼 중독에 유의미한 결과를 가져온 반면, 개방형은 부적인 상관관계를 나타냈다. 넷째, 마음의 창 유형이 스마트폰 중독에 의미 있는 영향을 미치는 변인인지에 대하여 검증한 결과 마음의 창 유형이 스마트폰 중독에 영향을 미치는 것을 알 수 있다. 이상의 결과를 바탕으로 스마트폰 중독에 가장 심각한 유형은 신중형과 고립형으로 나타났다. 그러므로 스마트폰 중독을 치료하는 프로그램 개발에 신중형과 고립형을 위한 구체적인 방안을 모색해야 될 것이다.