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http://dx.doi.org/10.5010/JPB.2008.35.4.329

Transgenic tobacco with γ-TMT of perilla showed increased salt resistance and altered pigment synthesis  

Woo, Hee-Jong (National Academy of Agricultural Science, Rural Development Administration (RDA))
Sung, Jwa-Kyung (National Academy of Agricultural Science, Rural Development Administration (RDA))
Kim, Jung-Bong (National Academy of Agricultural Science, Rural Development Administration (RDA))
Kim, Na-Young (National Academy of Agricultural Science, Rural Development Administration (RDA))
Lee, Si-Myung (National Academy of Agricultural Science, Rural Development Administration (RDA))
Shin, Kong-Sik (National Academy of Agricultural Science, Rural Development Administration (RDA))
Lim, Sun-Hyung (National Academy of Agricultural Science, Rural Development Administration (RDA))
Suh, Seok-Cheol (National Academy of Agricultural Science, Rural Development Administration (RDA))
Kim, Kyung-Hwan (Audit and Inspection Officer, Rural Development Administration (RDA))
Cho, Yong-Gu (Department of Crop Science, Chungbuk National University)
Publication Information
Journal of Plant Biotechnology / v.35, no.4, 2008 , pp. 329-335 More about this Journal
Abstract
Tocopherols are essential lipophilic antioxidant in human cells, while little is known about its function in plant tissues. To study the impact of composition and content of tocopherols on stress tolerance, tobacco (Nicotiana tabacum) was transformed with a construct containing a cDNA insert encoding $\gamma$-tocopherol methyltransferase ($\gamma$-TMT/VTE4) from perilla under the control of the cauliflower mosaic virus (CaMV) 35S promoter. The transgenic tobacco was confirmed by PCR and RT-PCR. The total content and composition of tocopherols in the transgenic lines were similar with wild type controls. However, chlorophyll-a and carotenoid content in the transgenic lines were increased by up to 45% (P<0.01) and 39% (P<0.02), respectively. Also, the over-expression of $\gamma$-TMT increased the salt stress tolerance in tobacco plants. These results demonstrate that over-expression of $\gamma$-TMT gene in tocopherol bio-synthetic pathway can increase salt stress tolerance and contents of chlorophyll-a and carotenoid in transgenic tobacco plants.
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