Journal of Plant Biotechnology

  • 제35권4호
  • /
  • Pages.329-335
  • /
  • 2008
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  • 1229-2818(pISSN)
  • /
  • 2384-1397(eISSN)
한국식물생명공학회 (The Korean Society of Plant Biotechnology)
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들깨 γ-TMT 형질전환 담배의 색소성분 변화 및 염 스트레스 내성 증가

Transgenic tobacco with γ-TMT of perilla showed increased salt resistance and altered pigment synthesis

  • 우희종 (농촌진흥청 국립농업과학원) ;
  • 성좌경 (농촌진흥청 국립농업과학원) ;
  • 김정봉 (농촌진흥청 국립농업과학원) ;
  • 김나영 (농촌진흥청 국립농업과학원) ;
  • 이시명 (농촌진흥청 국립농업과학원) ;
  • 신공식 (농촌진흥청 국립농업과학원) ;
  • 임선형 (농촌진흥청 국립농업과학원) ;
  • 서석철 (농촌진흥청 국립농업과학원) ;
  • 김경환 (농촌진흥청 감사담당관실) ;
  • 조용구 (충북대학교 식물자원학과)
  • Woo, Hee-Jong (National Academy of Agricultural Science, Rural Development Administration (RDA)) ;
  • Sung, Jwa-Kyung (National Academy of Agricultural Science, Rural Development Administration (RDA)) ;
  • Kim, Jung-Bong (National Academy of Agricultural Science, Rural Development Administration (RDA)) ;
  • Kim, Na-Young (National Academy of Agricultural Science, Rural Development Administration (RDA)) ;
  • Lee, Si-Myung (National Academy of Agricultural Science, Rural Development Administration (RDA)) ;
  • Shin, Kong-Sik (National Academy of Agricultural Science, Rural Development Administration (RDA)) ;
  • Lim, Sun-Hyung (National Academy of Agricultural Science, Rural Development Administration (RDA)) ;
  • Suh, Seok-Cheol (National Academy of Agricultural Science, Rural Development Administration (RDA)) ;
  • Kim, Kyung-Hwan (Audit and Inspection Officer, Rural Development Administration (RDA)) ;
  • Cho, Yong-Gu (Department of Crop Science, Chungbuk National University)
  • 발행 : 2008.12.31
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초록

Tocopherols are essential lipophilic antioxidant in human cells, while little is known about its function in plant tissues. To study the impact of composition and content of tocopherols on stress tolerance, tobacco (Nicotiana tabacum) was transformed with a construct containing a cDNA insert encoding $\gamma$-tocopherol methyltransferase ($\gamma$-TMT/VTE4) from perilla under the control of the cauliflower mosaic virus (CaMV) 35S promoter. The transgenic tobacco was confirmed by PCR and RT-PCR. The total content and composition of tocopherols in the transgenic lines were similar with wild type controls. However, chlorophyll-a and carotenoid content in the transgenic lines were increased by up to 45% (P<0.01) and 39% (P<0.02), respectively. Also, the over-expression of $\gamma$-TMT increased the salt stress tolerance in tobacco plants. These results demonstrate that over-expression of $\gamma$-TMT gene in tocopherol bio-synthetic pathway can increase salt stress tolerance and contents of chlorophyll-a and carotenoid in transgenic tobacco plants.

키워드

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피인용 문헌

  1. Strategies for developing marker-free transgenic plants vol.16, pp.6, 2011, https://doi.org/10.1007/s12257-011-0519-3