• 재활복지공학회논문지
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• 제11권2호
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• pp.187-198
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• 2017

이 연구에서는 우리나라의 모바일 앱 접근성 KS, 미국의 재활법 508조 개정 표준 및 유럽의 BBC 지침을 기준으로 개방형 플랫폼인 안드로이드 모바일 뱅킹 앱의 접근성을 분석하였다. 실험 결과, 우리나라 8개 시중은행의 모바일 뱅킹 앱 중 상기 3가지 표준의 세부 항목을 모두 만족하는 앱은 한 곳도 없었다. 대표적인 위반 항목은 대체 텍스트 미비, 키보드 초점 위반, 컨트롤 크기 및 간격 미준수, 명도대비 비율 낮음 등이었다. 이들 위반사례는 시각장애인, 상지장애인 및 저시력인과 고령자의 접근을 불가능하게 만든다는 점에서 치명적인 오류이다. 모바일 뱅킹 앱이 접근성을 준수하지 않는 원인에 있어서는 모바일 앱 개발자와 제공자들의 접근성에 관한 인식이 낮고 접근성 구현 방법을 제대로 숙지하지 못한 것으로 분석되었다. 우리나라의 모바일 앱 접근성 지침을 재활법 508조 개정 표준 및 BBC 표준과 비교한 결과, 많은 항목이 누락되어 있었다. 또한 평가 기준이 모호하고 추상적이어서 개발자들이 구체적으로 참고하기 어려운 측면이 있는 것으로 판단되었다. 따라서 모바일 앱 접근성 향상을 위한 개발자 및 정부의 노력과 더불어 표준의 보완이 필요하다.

• Bulletin of the Korean Chemical Society
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• 제31권10호
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• pp.2873-2876
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• 2010

Excess free iron generates oxidative stress that may contribute to the pathogenesis of various causes of neurodegenerative diseases. Previous studies have shown that one of the primary causes of increased brain iron may be the release of excess iron from intracellular iron storage molecules. In this study, we attempted to characterize the oxidative damage of DNA induced by the reaction of ferritin with $H_2O_2$. When DNA was incubated with ferritin and $H_2O_2$, DNA strand breakage increased in a time-dependent manner. Hydroxyl radical scavengers strongly inhibited the ferritin/$H_2O_2$ system-induced DNA cleavage. We investigated the generation of hydroxyl radical in the reaction of ferritin with $H_2O_2$ using a chromogen, 2,2'-azinobis-(2-ethylbenzthiazoline-6-sulfonate) (ABTS), which reacted with ${\cdot}OH$ to form $ABTS^{+\cdot}$. The initial rate of $ABTS^{+\cdot}$ formation increased as a function of incubation time. These results suggest that DNA strand breakage is mediated in the reaction of ferritin with $H_2O_2$ via the generation of hydroxyl radicals. The iron-specific chelator, deferoxamine, also inhibited DNA cleavage. Spectrophotometric study using a color reagent showed that the release of iron from $H_2O_2$-treated ferritin increased in a time-dependent manner. Ferritin enhanced mutation of the lacZ' gene in the presence of $H_2O_2$ when measured as a loss of $\alpha$-complementation. These results indicate that ferritin/$H_2O_2$ system-mediated DNA cleavage and mutation may be attributable to hydroxyl radical generation via a Fenton-like reaction of free iron ions released from oxidatively damaged ferritin.

• Journal of Microbiology and Biotechnology
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• 제13권2호
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• pp.292-300
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• 2003

Pseudomonas fluorescens B16 is a plant glowth-prornoting rhizobacterium, which produces an antibacterial compound that is effective against plant root pathogens, such as Agrobacrerium tumefaciens and Raistonia solanacearum. We mutagenized the strain B16 with Omegon-Km and isolated six antibacterial-activity-deficient mutants. Two cosmid clones that hybridized with the mutant clones also were isolated from a genomic library of tile parent strain. Using deletion and complementation analyses, it was found that the biosynthesis genes resided in a 4.3-kb SalI-NarI fragment. When a plasmid clone carrying the fragment was introduced into P. fluorescens strain 1855.344, which does not exhibit any antibacterial activity, the transconjugants exhibited antibacterial activity, indicating that the plasmid clone carried all the genes essential for production of the antibacterial compound. DNA sequence analysis of the fragment identified four putative open reading frames (ORFs): orf1 through orf4 The deduced amino acid sequences of ORF1, ORF2, and ORF4 were similar to cystathionine gamma lyase, pyruvate formate-lyase activating enzyme, and transcriptional regulator, respectively, yet the amino acid sequence of ORF3 showed no similarities to any known proteins. It was also demonstrated that the antibacterial activity was responsible for biological control of the bacterial wilt caused by R. solanacearum.

• Journal of Microbiology
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• 제37권4호
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• pp.248-255
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• 1999

We have analyzed the MRE3/REC114 gene of Saccharomyces cerevisiae, previously detected in isolation of mutants defective in meiotic recombination. We cloned the MRE3/REC114 gene by complementation of the meiotic recombination defect and it has been mapped to chormosome XIII. The DNA sequence analysis revealed that the MRE3 gene is identical to the REC114 gene. The upstream region of the MRE3/REC114 gene contains a T_4C site, a URS (upstream repression sequence) and a TR (T-rich) box-like sequence, which reside upstream of many meiotic genes. Coincidentally, northern blot analysis indicated that the three sizes of MRE3/REC114 transcripts, 3.4, 1.4 and 1.2 kb, are induced in meiosis. A less abundant transcript of 1.4 kb is detected in both mitotic and meiotic cells, suggesting that it is needed in mitosis as well as meiosis. To examine the role of the MRE3/REC114 gene, we constructed mre3 disruption mutants. Strains carrying an insertion or null deletion of the MRE3/REC114 gene showed slow growth in nutrient medium and the doubling time of these cells increased approximately by 2-fond compared to the wild-type strain. Moreover, the deletion mutant (${\delta}$mre3) displayed no meiotically induced recombination and no viable spores. The mre3/rec114 spore lethality can be suppressed by spo13, a mutation that causes cells to bypass reductional division. The double-stranded breaks (DSBs) which are involved in initiation of meiotic recombination were not detected in the analysis of meiotic chromosomal DNA from the mre3/rec114 disruptant. From these results we suggest that the MRE3/REC114 gene product is essential in normal growth and in early meiotic stages involved in meiotic recombination.

• The Plant Pathology Journal
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• 제37권3호
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• pp.232-242
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• 2021

Glomerella leaf spot (GLS) is a severe infectious disease of apple whose infective area is growing gradually and thus poses a huge economic threat to the world. Different species of Colletotrichum including Colletotrichum gloeosporioides are responsible for GLS. For efficient GLS control, it is important to understand the mechanism by which the cruciferous crops and C. gloeosporioides interact. Arginine is among one of the several types of amino acids, which plays crucial role in biochemical and physiological functions of fungi. The arginine biosynthesis pathway involved in virulence among plant pathogenic fungi is poorly understood. In this study, CgCPS1 gene encoding carbamoyl phosphate synthase involved in arginine biosynthesis has been identified and inactivated experimentally. To assess the effects of CgCPS1, we knocked out CgCPS1 in C. gloeosporioides and evaluated its effects on virulence and stress tolerance. The results showed that deletion of CgCPS1 resulted in loss of pathogenicity. The ∆cgcps1 mutants showed slow growth rate, defects in appressorium formation and failed to develop lesions on apple leaves and fruits leading to loss of virulence while complementation strain (CgCPS1-C) fully restored its pathogenicity. Furthermore, mutant strains showed extreme sensitivity to high osmotic stress displaying that CgCPS1 plays a vital role in stress response. These findings suggest that CgCPS1 is major factor that mediates pathogenicity in C. gloeosporioides by encoding carbamoyl phosphate that is involved in arginine biosynthesis and conferring virulence in C. gloeosporioides.

• Journal of Microbiology
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• 제41권3호
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• pp.212-218
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• 2003

Citrus phytoene synthase (CitPsy) and ${\beta}$-carotene hydroxylase (CitChx), which are involved in caroteinoid biosynthesis, are distantly related to the corresponding bacterial enzymes from the point of view of amino acid sequence similarity. We investigated these enzyme activities using Pantoea ananatis carotenoid biosynthetic genes and Escherichia coli as a host cell. The genes were cloned into two vector systems controlled by the T7 promoter. SDS-polyacrylamide gel electrophoresis showed that CitPsy and CitChx proteins are normally expressed in E. coli in both soluble and insoluble forms. In vivo complementation using the Pantoea ananatis enzymes and HPLC analysis showed that ${\beta}$-carotene and zeaxanthin were produced in recombinant E. coli, which indicated that the citrus enzymes were functionally expressed in E. coli and assembled into a functional multi-enzyme complex with Pantoea ananatis enzymes. These observed activities well matched the results of other researchers on tomato phytoene synthase and Arabidopsis and pepper ${\beta}$-carotene hydroxylases. Thus, our results suggest that plant carotenoid biosynthetic enzymes can generally complement the bacterial enzymes and could be a means of carotenoid production by molecular breeding and fermentation in bacterial and plant systems.

• 한국조경학회지
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• 제30권1호
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• pp.11-17
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• 2002

This paper compares the difference between the village groves in the Jinan settlement region and the Hamyang settlment region in Honam and the Youngnam mountainous region. The results are as follows: Fifty-one village groves in the Jinan legion were found and 40 village groves were found in the Hamyang region. It was established that the village groves in the Jinan region of Honam province were conserved better than those of Hamyang region of Youngnam province. In the Jinan area, the village groves were principally found in the Jinan-eup and the Hamyang regions, primarily in Seosang-myun and Aneui-myun. So it was determined that the village groves were distributed inequitably in the two regions. In L / W ratio, the Jinan region is 3.6 and Hamyang region is 2.0 Jinan is 1.8 times greater than Hamyang. Therefore, it was concluded that Jinan is stronger than Hamyang in linear function of the village groves. Stone stooge for complementation function was found in 11 village groves in the Jinan region and 6 village groves in Hamyang. So the percentage of the remaining stone storage is 15-20 % of all village groves. Regarding grove makeup, the village gloves in Jinan region is deciduous forest principally. In contrast, the groves of Hamyang are principally coniferous forest. The dominant tree of the Jinan-gun region is Zelkova serrata, but in Hamyang it is Pinus densiflrora. By number, Hamyang region has two times the trees than Jinan region. In density of stand, the Hamyang region is 76.2% higher than Jinan region. In height and diameter of trees, the Jinan region is greater than the Hamyang region. It was identified that RSI of both region is 40%. So in density of trees, both region are sparse. This paper has a limit in ideiltiting the differences between Yolmg-nam ar\ulcorner Hnam village groves because comparative review only examined two regions.

• 한국작물학회:학술대회논문집
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• 한국작물학회 2017년도 9th Asian Crop Science Association conference
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• pp.110-110
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• 2017

Stomata are the main gateways for water and air transport between leaves and the environment. Inward-rectifying potassium channels regulate photo-induced stomatal opening. Rice contains three inward rectifying shaker-like potassium channel proteins, OsKAT1, OsKAT2 and OsKAT3. Among these, only OsKAT2 is specifically expressed in guard cells. Here, we investigated the functions of OsKAT2 in stomatal regulation using three dominant negative mutant proteins, OsKAT2(T235R), OsKAT2(T285A) and OsKAT2(T285D), which are altered in amino acids in the channel pore and at a phosphorylation site. Yeast complementation and patch clamp assays showed that all three mutant proteins lost channel activity. However, among plants overexpressing these mutant proteins, only plants overexpressing OsKAT2(T235R) showed significantly less water loss than the control. Moreover, overexpression of this mutant protein led to delayed photo-induced stomatal opening and increased drought tolerance. Our results indicate that OsKAT2 is an inward-rectifying shaker-like potassium channel that mainly functions in stomatal opening. Interestingly, overexpression of OsKAT2(T235R) did not cause serious defects in growth or yield in rice, suggesting that OsKAT2 is a potential target for engineering plants with improved drought tolerance without yield penalty.

• Journal of Microbiology and Biotechnology
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• 제26권12호
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• pp.2106-2115
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• 2016

To identify the global effects of (p)ppGpp in the gram-positive bacterium Deinococcus radiodurans, which exhibits remarkable resistance to radiation and other stresses, RelA/SpoT homolog (RSHs) mutants were constructed by direct deletion mutagenesis. The results showed that RelA has both synthesis and hydrolysis domains of (p)ppGpp, whereas RelQ only synthesizes (p)ppGpp in D. radiodurans. The growth assay for mutants and complementation analysis revealed that deletion of relA and relQ sensitized the cells to $H_2O_2$, heat shock, and amino acid limitation. Comparative proteomic analysis revealed that the bifunctional RelA is involved in DNA repair, molecular chaperone functions, transcription, the tricarboxylic acid cycle, and metabolism, suggesting that relA maintains the cellular (p)ppGpp levels and plays a crucial role in oxidative resistance in D. radiodurans. The D. radiodurans relA and relQ genes are responsible for (p)ppGpp synthesis/hydrolysis and (p)ppGpp hydrolysis, respectively. (p)ppGpp integrates a general stress response with a targeted re-programming of gene regulation to allow bacteria to respond appropriately towards heat shock, oxidative stress, and starvation. This is the first identification of RelA and RelQ involvement in response to oxidative, heat shock, and starvation stresses in D. radiodurans, which further elucidates the remarkable resistance of this bacterium to stresses.

• 한국측량학회:학술대회논문집
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• 한국측량학회 2004년도 추계학술발표회 논문집
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• pp.405-410
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• 2004

In mid-90's, the Korean government introduced the GIS(Geographic Information System) to digitalize every topography of national land and thereby, index locations and attributes of various urban facilities to construct a system whereby every information could be managed and operated in an integrated way, but the reliability of such geographic information has yet to be tested, much less its modification, complementation and maintenance. Under such circumstances, this study was aimed at constructing a reference point infrastructure for Seoul and Kyonggi area and comparing the data obtained from the GPS operation and various facility location data with the existing GIS data to address the problems of GIS operation and suggest their solutions. As a result of calculating the GPS reference point data and analyzing the deviations of the unknown point data in comparison with the fixed point ones, it was found that the horizontal location values were reliable within +/- 5cm, but that the above-sea level values varied as much as 1.4m depending on the deployment of pre-set fixed points determined by the direct level gauging. In addition, as a result of directly surveying major facilities around the roads based on the coordinates of the urban reference points networked with such a reference point system to check their conformity to existing data, it was confirmed that the difference was as wide as 2m. Such differences may be attributable to the fact that the data with their geographic information not confirmed are used as basic data for GIS. Hence, this study suggests the ways to set the absolute geographic data based on reference points and test the reliability of existing data and thereby, suggests a methods to solve the problems.