• 한국동물위생학회지
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• 제43권1호
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• pp.17-21
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• 2020

It was carried out to investigate the antibiotic residues detected by the residue tests for edible tissues of slaughtered livestock in Korea from January to November in 2019. Positive cases of qualitative test and analytical test for 11 months were 689 and 341, respectively. Positive cases of analytical test for cattle, pigs, horses and poultry were 164, 168, 2 and 7, respectively. Antibiotics were detected from 472 materials from 341 cases and classified as β-lactams 162 (37.9%), aminoglycosides 141 (33%), quinolones 69 (16.2%), sulfonamides 23 (5.4%), amphenicols 10 (2.3%), tetracyclines 9 (2.1%), macrolides 6 (1.4%) and the rest 7 (1.4%). Other materials were benzyl penicillin 119 (27.9%), (dihydro)streptomycin 105 (24.6%) and enrofloxacin (including ciprofloxacin) 39 (9.1%). In conclusion, strict management of benzyl penicillin and streptomycin in cattle and pigs should be implemented to reduce the positive cases of slaughtered livestock in Korea.

• 한국동물위생학회지
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• 제26권2호
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• pp.113-119
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• 2003

This study was carried out to compare the antibiotic residues in the muscles of cattle and pigs from slaughter-houses in Seoul from 2000 to 2002 by EEC-4-plate, Charm II and HPLC methods. The results were summarized as follows; 1. Residual materials were detected from 41 samples(0.6%) by EEC-4-plate method from random sampling and 38 samples(12%) by Charm II method from directed sampling. 2. Violation rates were 0.3% by monitoring and 4.7% by surveillance program. 3. The 35 samples were classified as tetracyclines 30(86%), sulfonamides 4(11%), ${\beta}$-lactams 1(3%) and two samples simultaneously determined oxyteracycline plus sulfadimetoxine, and sulfamerazine plus sulfadimetoxine. 4. The highest residual concentration(ppm) of chlortetracycline, oxytetracyline, sulfamethazine, sufadimetoxine and penicillin were 0.5, 12.0, 6.4, 2.6 and 0.44, respectively.

• 한국동물위생학회지
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• 제32권1호
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• pp.93-102
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• 2009

In this study, antimicrobial resistance of E. coli isolated from domestic beef on sale in Busan and Gyeongnam province was investigated from March to October 2008. A total of 400 beef samples were collected for the monitoring of antimicrobial resistance, and 39 (9.8%) strains of E. coli were isolated. Antimicrobial resistance test was carried out by agar disc diffusion method with 17 antimicrobials. In general, E. coli isolates showed the highest antimicrobial resistance to tetracycline (85.3%), followed by doxycycline (76.5%), streptomycin (61.8%) and sulfamethoxazole/trimethoprim (61.8%). Then they showed higher resistance to several antimicrobials like kanamycin and neomycin (55.9%). However, They had low antimicrobial resistance to amikacin (8.8%), amoxicillin/clavulanic acid (2.9%). Of 39 isolates, 31 (79.5%) were resistant to more than 2 antimicrobials. Among 17 antimicrobials examined, tetracyclines were the most resistant, followed by aminoglycosides, sulfonamides. The resistance was seemed to be correlated to amounts of antimicrobial use. In the result of this study, we suggest that there be need to regulate the abuse of antimicrobial on food-producing animals in Korea because the concern on antimicrobial resistant is gradually increased worldwide.

• 대한의사협회지
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• 제61권11호
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• pp.680-686
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• 2018

Acne vulgaris is a very common condition affecting up of about 80% to 90% of adolescents. The patients with acne have been shown to be adversely impacted by the effect of acne on their quality of life. Four factors are believed to play a key role in the development of acne lesions: excess sebum production, disturbed keratinization within the follicle, colonization of the pilosebaceous duct by Propionibacterium acnes, and the release of inflammatory mediators into the skin. Consequently, the target for acne therapy is these well-known pathogenic factors responsible for this disease state. Topical retinoids correct abnormal keratinization, but it should be applied cautiously because of irritation. Benzoyl peroxide is an effective bactericidal agent against P. acnes. Main topical antibiotics are erythromycin and clindamycin. Fixed combination topical products with retinoids, benzoyl peroxide and antibiotics have been introduced. Use of systemic antibiotics, including tetracyclines and macrolides rapidly improves inflammatory acne lesions. Oral isotretinoin is effective against all of the main pathogenic features of acne but is contraindicated in pregnant women and has been associated with cheilitis and dry skin. Hormonal therapy has been found to improve acne in some selective patients and should be considered for appropriate candidates. This review will present the general aspects of the pharmacological treatments for acne.

• Clinical and Experimental Pediatrics
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• 제62권6호
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• pp.199-205
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• 2019

Although Mycoplasma pneumoniae pneumonia (MPP) has been generally susceptible to macrolides, the emergence of macrolide-resistant MPP (MRMP) has made its treatment challenging. MRMP rapidly spread after the 2000s, especially in East Asia. MRMP is more common in children and adolescents than in adults, which is likely related to the frequent use of macrolides for treating M. pneumoniae infections in children. MRMP is unlikely to be related to clinical, laboratory, or radiological severity, although it likely prolongs the persistence of symptoms and the length of hospital stay. Thereby, it causes an increased burden of the disease and poor quality of life for the patient as well as a societal socioeconomic burden. To date, the only alternative treatments for MRMP are secondary antimicrobials such as tetracyclines (TCs) or fluoroquinolones (FQs) or systemic corticosteroids; however, the former are contraindicated in children because of concerns about potential adverse events (i.e., tooth discoloration or tendinopathy). A few guidelines recommended TCs or FQs as the second-line drug of choice for treating MRMP. However, there have been no evidence-based guidelines. Furthermore, safety issues have not yet been resolved. Therefore, this article aimed to review the benefits and risks of therapeutic alternatives for treating MRMP in children and review the recommendations of international or regional guidelines and specific considerations for their practical application.

• Clinical and Experimental Pediatrics
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• 제63권12호
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• pp.469-476
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• 2020

The major pathogens that cause atypical pneumonia are Mycoplasma pneumoniae, Chlamydophila pneumoniae, and Legionella pneumophila. Community-acquired pneumonia (CAP) caused by M. pneumoniae or C. pneumoniae is common in children and presents as a relatively mild and self-limiting disease. CAP due to L. pneumophila is very rare in children and progresses rapidly, with fatal outcomes if not treated early. M. pneumoniae, C. pneumoniae, and L. pneumophila have no cell walls; therefore, they do not respond to β-lactam antibiotics. Accordingly, macrolides, tetracyclines, and fluoroquinolones are the treatments of choice for atypical pneumonia. Macrolides are the first-line antibiotics used in children because of their low minimum inhibitory concentrations and high safety. The incidence of pneumonia caused by macrolide-resistant M. pneumoniae that harbors point mutations has been increasing since 2000, particularly in Korea, Japan, and China. The marked increase in macrolide-resistant M. pneumoniae pneumonia (MRMP) is partly attributed to the excessive use of macrolides. MRMP does not always lead to clinical nonresponsiveness to macrolides. Furthermore, severe complicated MRMP responds to corticosteroids without requiring a change in antibiotic. This implies that the hyper-inflammatory status of the host can induce clinically refractory pneumonia regardless of mutation. Empirical macrolide therapy in children with mild to moderate CAP, particularly during periods without M. pneumoniae epidemics, may not provide additional benefits over β-lactam monotherapy and can increase the risk of MRMP.

• 멤브레인
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• 제34권2호
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• pp.96-104
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• 2024

항생제는 과도한 사용으로 인해 폐수뿐만 아니라 다양한 수원에서 발견되는 새로운 오염 물질 중 하나입니다. 수중 항생제 오염 물질을 처리하기 위한 고도 산화 공정, 생물학적 처리 등 다양한 기술이 있습니다. 이 두 가지 공정은 비효율적이며, 부산물의 생성은 이 공정을 더욱 복잡하게 만듭니다. 오염 물질을 제거하기 위한 또 다른 대안으로 막 기술이 있습니다. 항생제와 내성 유전자의 제거를 개선하기 위해 막 생물 반응기는 NaClO와 탄소 물질로 변형됩니다. 풍부한 반응성 종의 생성은 항생제의 내성 유전자에 대해 활성입니다.

• 한국환경보건학회지
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• 제32권3호
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• pp.227-234
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• 2006

There has been increasing concern in recent years about the occurrence, fate and toxicity of pharmaceutical products in aquatic environment. Although these compounds have been detected in a wide variety of environmental samples including sewage effluent, surface waters, ground water and drinking water, their concentrations generally range from the low ppt to ppb levels. It is therefore often thought to be unlikely that pharmaceuticals will have a detrimental effect on the environment. This study was conducted to determine the endocrine disruption effects of the several pharmaceutical residues in water using adult Japanese medaka (Oryzias latipes). The common antibiotics were used sulfa durgs (sulfamethoxazole and sulfamethazine) and tetracycline drugs (oxytetracycline and tetracycline). Positive control that was induced Vtg (vitellogenin) in male fish was used $17\beta$-estradiol. Vtg was qualified and quantified through Western blotting and ELISA. After SDS gel electrophoresis, the dominant protein band was identified to molecular weight approximately 205 kDa in whole body samples of vitellogenic female. In female medaka exposed to $17\beta$estradiol, there was no significant difference in total protein induction. In contrast, three to five day exposure of male fish to $17\beta$-estradiol resulted in more than 60.0% increase of total protein compared to that of control males (p<0.01). In case of antibiotics, female fish didn't show significant difference, but male fish was showed significant difference. In addition, Vtg induction in male fish was observed with all the test chemicals. On concentrations greater than 0.1 ppm of sulfamethoxazole, 1 ppm of sulfamethazine, 1 ppm of oxytetracycline and 20 ppm of tetracycline, Vtg induction was increased in a dose response manner. This study is one of the early reports suggesting potential endocrine disruption mechanism of antibiotic pharmaceutical products in aquatic ecosystem. Although the effect concentrations obtained from this study were high as unrealistically as in environments, it is endocrine disruption that we should be considered as one of the important consequences of pharmaceutical contamination at water environment, and warrants due attention in future researches.

• 한국식품위생안전성학회지
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• 제5권3호
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• pp.159-164
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• 1990

To detect and quantitation residual antibiotics and antibacterial agents in meats, we performed a biological assay employing the three microorganisms Bacillus subtilis ATCC 6633, Micrococcus luteus ATCC 9341, and Bacillus cereus var. mycoides ATCC 11778 for the screening purpose and developed a Gas Chromatography-mass Spectrometry(GC/MS) analysis for the confirmation and quantiation. In the biological assay (paper disk method), three test solution are used depending on the character of the residual antibiotics and antibacterial agents, follow by a simple clean up procedure which includes homogenization with Mcilvaine buffer, defatting with includes homogenization with Mcilvaine buffer, defatting with hexane, extraction with chloroform, clean-up by Sep-Pak $C_{18}$ and Bakerbond SPE carboxylic acid column. The chloroform layer is used for the analysis of sulfa agents. macrolides antibiotics and antibacterial agents, Adsorbed materials in the Sep-Pak $C_{18}$ were also employed for th analysis of penicillins and tetracyclines. Effluents from the Sep-Pak $C_{18}$ were cleaned-up one more by Bakerbond 10 SPE COOH column and employed for the analysis of aminoglycosides. In the instrumental analysis by using the GC/MSD, residual antibiotics and antibacterial agent were quantitated by selected ion monitoring (SIM) mode after derivatization. A simultaneous analysis of six residual antibiotic and antibacterial agent such as oxytetracycline, penicillin, ampicillin, choliraphenicol and thiamphenicol was developed with simple cleanup procedures revealing good recovery and reproducibility. Also, simultaneous detection of macrolides antibiotics such as erythromycin, spiramycin, and oleandomycin was developed after acid hydrolysis due to their large molecular structures. Because of the high reproducibility and selectivity of these two methods, it is very desirable that the combination of the two methods be used in the bioassay for the screening of residual antibiotics and antibacterial agent and that GC/MSD analysis be used for the confirmation and quantitation.

• 한국토양비료학회지
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• 제51권1호
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• pp.8-15
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• 2018

A portion of the veterinary antibiotics administrated to livestock are generally excreted via feces and urine. Tetracyclines and tylosin have a greater priority of environmental risk in Korea based on the consumption and the potential to reach soil and water environment. The antibiotics in animal byproducts need to be reduced or eliminated before they are applied to agricultural lands through composting or other agricultural practices. The objective of the study was to investigate the effect of aeration on degradation of antibiotics during storage of swine slurry. Two antibiotics, tetracycline (TC) and tylosin (TYL), were detected from the swine slurry used in the study. One hour aeration per day for 62 days reduced TC concentration from 199 to $43ng\;L^{-1}$ compared with $104ng\;L^{-1}$ without aeration. Aeration for three and six hours decreased TC level to 30 and $23ng\;L^{-1}$, respectively. The dissipation of TC was fitted with a first-order kinetic model. Aeration for 1, 3, and 6 hours every day increased the first-order rate constant, k, from $0.011day^{-1}$ under anaerobic condition to 0.022, 0.026, and $0.037day^{-1}$, respectively. For TYL, aeration during storage of swine slurry enhanced k from $0.0074day^{-1}$ to 0.014, 0.018, and $0.031day^{-1}$ for 1, 3, and 6 hours per day, respectively. For liquid swine slurry, biotic processes can be more effective for dissipation of antibiotics than abiotic processes because of low organic matter and high water content. These results suggest that aeration can increase the degradation rate of antibiotics during storage of swine slurry.