• Journal of Microbiology and Biotechnology
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• v.19 no.11
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• pp.1306-1318
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• 2009

The filamentous ascomycete Sclerotinia sclerotiorum is well known for its ability to produce a large variety of hydrolytic enzymes. Two $\alpha$-amylases ScAmy54 and ScAmy43 predicted to play an important role in starch degradation were showed to produce specific oligosaccharides essentially maltotriose that have a considerable commercial interest. Primary structure of the two enzymes was established by N-terminal sequencing, MALDI-TOF masse spectrometry and cDNA cloning. The two proteins have the same N-terminal catalytic domain and ScAmy43 derived from ScAmy54 by truncation of 96 amino acids at the carboxyl-terminal region. Data of genomic analysis suggested that the two enzymes originated from the same $\alpha$-amylase gene and that truncation of ScAmy54 to ScAmy43 occurred probably during S. sclerotiorum cultivation. The structural gene of Scamy54 consisted of 9 exons and 8 introns, containing a single 1,500-bp open reading frame encoding 499 amino acids including a signal peptide of 21 residues. ScAmy54 exhibited high amino acid homology with other liquefying fungal $\alpha$-amylases essentially in the four conserved regions and in the putative catalytic triad. A 3D structure model of ScAmy54 and ScAmy43 was built using the 3-D structure of 2guy from A. niger as template. ScAmy54 is composed by three domains A, B, and C, including the well-known $(\beta/\alpha)_8$ barrel motif in domain A, have a typical structure of $\alpha$-amylase family, whereas ScAmy43 contained only tow domains A and B is the first fungal $\alpha$-amylase described until now with the smallest catalytic domain.

• The Plant Pathology Journal
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• v.28 no.4
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• pp.409-417
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• 2012

We identified two novel expansin (EXP) genes in the expressed sequence tag database of Bursaphelenchus xylophilus, designated as Bx-EXPB2 and -EXPB3. Novel Bx-EXPBs encoded 150 amino acids and their similarities in coding sequence were 70.7-84.0% to the previously reported EXPB1 of B. xylophilus. Bx-EXPB2 and Bx-EXPB3 were clustered with Bx-EXPB1 and Bm-EXPB1, respectively, forming the independent phylogeny with other nematode EXPs. All identified Bx-EXPBs contained the signal peptide and were only expressed during the propagative stage, suggesting that they are secreted to facilitate nematode migration through hosts by loosening cell walls during infection. Quantitative real-time PCR analysis showed that the relative accumulation of Bx-EXPB3 mRNAs was the highest among the three Bx-EXPs examined and the order of mRNA accumulation was as follows: Bx-EXPB3 > Bx-EXPB2 >> Bx-EXPB1. Homology modeling of Bx-EXPBs showed that the structurally optimum template was EXLX1 protein of Bacillus subtilis, whichshared residues essential for catalytic activity with Bx-EXPB1 and Bx-EXPB2 except for Bx-EXPB3. Taken together, Bx-EXPB1 and Bx-EXPB2 may be involved migration through plant tissues and play a role in pathogenesis.

• Journal of the Korea Safety Management & Science
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• v.9 no.6
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• pp.123-135
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• 2007

In the recent years, the companies have manually recorded a production status in a work diary or have mainly used a bar code in order to collect each process's progress status, production performance and quality information in the production and logistics process in real time. But, it requires an additional work because the worker's record must be daily checked or the worker must read it with the bar code scanner. At this time, data's accuracy is decreased owing to the worker's intention or mistake, and it causes the problem of the system's reliability. Accordingly, in order to solve such problem, the companies have introduced RFID which comes into the spotlight in the latest automatic identification field. In order to introduce the RFID technology, the process flow must be analyzed, but the ASME sign used by most manufacturing companies has the difficult problem when the aggregation event occurs. Hence, in this study, the RFID logistic flow analysis Modeling Notation was proposed as the signature which can analyze the manufacturing logistic flow amicably, and the manufacturing logistic flow by industry type was analyzed by using the proposed RFID logistic flow analysis signature. Also, to monitor real-time information through EPCglobal network, EPCISEvent template by industry was proposed, and it was utilized as the benchmarking case of companies for RFID introduction. This study suggested to ensure the decision-making on real-time information through EPCglobal network. This study is intended to suggest the Modeling Notation suitable for RFID characteristics, and the study is intended to establish the business step and to present the vocabulary.

• The Journal of Korean Institute of Electromagnetic Engineering and Science
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• v.15 no.10 s.89
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• pp.983-995
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• 2004

This paper introduces a pre-compensation filter for compensating dispersive and resonant properties experienced along the usage of non-ideal wideband antennas in impulse communication systems. It has been well blown that the transmitted impulse signal becomes deformed because of dispersive and resonant characteristics. Accordingly, in spite of using ideal template signal at the correlator in coherent receiver, these impairments degrade overall performance attributed to low level of coherence. To overcome this problem this paper exploits a realization technique of pre-compensation filter purposely installed at transmitter whose stability is automatically guaranteed because it has an inversion form of minimum-phase ARMA (Auto-Regressive Moving Average) system. The performance of proposed scheme will be shown in results from computer simulations to verify its affirmative impact on impulse communication system with regarding several distinctively shaped antennas.

• Korean Journal of Computational Design and Engineering
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• v.20 no.2
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• pp.145-158
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• 2015

ISO 15926 is an international standard for the sharing and integration of plant lifecycle information. Plant design data consist of logical configuration, equipment specifications, 2D piping and instrument diagrams (P&IDs), and 3D plant models (shape data). Although 3D computer-aided design (CAD) data is very important data across the plant lifecycle, few studies on the exchange of 3D CAD data using ISO 15926 have been conducted so far. For this, we analyze information requirements regarding plant 3D design in the process industry. Based on the analysis, ISO 15926 templates are defined for the representation of constructive solid geometry (CSG) - based 3D design data. Since system environments for 3D CAD modeling and Semantic Web technologies are different from each other, we present system architecture for processing and visualizing plant 3D design data in the Web Ontology Language (OWL) format. Through the visualization test of ISO 15926-based 3D design data for equipment with a prototype system, feasibility of the proposed method is verified.

• International Journal of Industrial Entomology and Biomaterials
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• v.10 no.1
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• pp.25-33
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• 2005

Recently a cDNA clone of apoLp-III from Hyphantria cunea was isolated and subjected to computational analysis to compare with other available sequences. Multiple sequence alignments were carried out using the amino acid sequences of apoLp-III from six insects. It was found that the H. cunea apoLp-III has relatively high sequence identities to Spodoptera litura ($69.5\%$), Manduca sexta ($66.8\%$), Galleria mellonella ($65.1\%$), Bombyx mori N4 ($54.3\%$) but less identity to Locusta migratoria ($18.3\%$). The amino acid composition was compared with other insects using EXPASY tools; it shows that alanine (Ala), glutamine (Gln), leucine (Leu) and lysine (Lys) are the major amino acid components of apoLp-III in H. cunea as well as other lepidopterans. Homology modeling performed using PSI-BLAST (PDB template M. sexta) reveals that the apoLp-III molecules consist of five, long amphipathic alpha helical bundles with short loops connecting the helices and shows homology with other insects. Phylogenetic analysis shows that the orthopteran apoLp-III represented by locust was most distantly related to the lepidopteran insects.

• Korean Journal of Computational Design and Engineering
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• v.17 no.1
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• pp.17-25
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• 2012

Recently, requests for accurate process planning using simulation have been increasing in many engineering fields including the shipbuilding industry, and many application systems for simulation have been developed. It is difficult, however, for a user to reuse the developed systems, because simulation models in the system are defined by its own method. In addition, the simulation model should be modified whenever a simulation sequence, which is called simulation scenario, is changed. Therefore, in this study, an elementary simulation object is proposed to modularize a simulation model. And the management scheme of simulation scenario is proposed to manage the scenario outside of the simulation models. Also, a simulation template is proposed to increase the development efficiency. To verify the efficiency of the proposed methods, application examples for shipbuilding process planning are implemented.

• Journal of Integrative Natural Science
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• v.8 no.2
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• pp.89-98
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• 2015

Chemoattractant receptor homologous molecule expressed on Th2 cells (CRTh2) is a G-protein coupled receptor targeted for inflammatory diseases such as asthma, allergic rhinitis and atopic dermatitis. In this study, pharmacophore modeling and comparative molecular field analysis (CoMFA) were performed on the series of 2-(2-(benzylthio)-1H-benzo[d]imidazol-1-yl) acetic acids derivatives. Five highly active compounds were used for generation of pharmacophore models using GASP module. The best pharmacophore model was selected and used as template for the alignment of compounds which was used for CoMFA analysis. The best predictions obtained for CoMFA was $q^2=0.545$, $r^2=0.756$. The predictive ability of the model was investigated using 15 test set compounds. Contour maps suggested that presence of bulky substituents at $5^{th}$ position of benzene ring connected to suphur atoms attached to imidazol ring will increase the activity of the compounds. The results obtained from this study will be useful to design more potent CRTh2 antagonists.

• Korean Journal of Computational Design and Engineering
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• v.21 no.3
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• pp.242-251
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• 2016

Around the world, BIM application has been mandated. In Korea, an announcement was made in 2016 that BIM application will be required for all public constructions ordered by the Public Procurement Service. However, BIM cannot be used for building permits as Seumter, a building permit system, doesn't support BIM, even if it was used for building design. To utilize BIM for permit application, this study introduces a method of management for BIM-used permit application information. It was done by analyzing the data entered for new building permit application in Seumter. For the analysis, the permit application information is classified as internal BIM information and external BIM information and the required information is defined. To easily create and utilize the data types at the design stage, this study introduces a method of creating and utilizing a permit template. Also, this study developed a tool for combining the area information to add up all the related information such as gross floor area and floor area ratio. This study is significant in that it analyzes the required information for new building permit application among all the permit application types in Korea.

• Journal of the Korean Institute of Landscape Architecture
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• v.27 no.4
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• pp.143-150
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• 1999

The purpose of this research is to find som ways to model tree forms more efficiently in reference with surveying structural data and handling parameters in plant Editor of AccuRender, the AutoCAD-based rendering software adopting the procedural plant modeling technique. In case of modelling a new tree, because it is efficient to modify an existing tree data as a template, we attempted to classify 81 species' data from existing plant library including conifers and deciduous tree. According to the qualitative characteristics and quantitative parameters of geometrical and branching structure, 8 types of tree form were classified with factor and cluster analysis. Some critical aspects found in the distributions of standardized scores of parameters in each type were discussed for explaining the tree forms intuitively. For adaptability of the resulted classification and typical parameters, 10 species of tree were measured and modelled, and proved to be very similar to the real structures of tree forms. CG or CAD-based plant modelling technique would be recommended not only as a presentation tool but for planting design, landscape simulation and assessment.