• Genomics & Informatics
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• 제18권4호
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• pp.40.1-40.8
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• 2020

Avian influenza (AIV) outbreaks can induce fatal human pulmonary infections in addition to economic losses to the poultry industry. In this study, we aimed to develop a rapid and sensitive point-of-care AIV test using loop-mediated isothermal amplification (LAMP) technology. We designed three sets of reverse transcription LAMP (RT-LAMP) primers targeting the matrix (M) and hemagglutinin (HA) genes of the H5 and H9 subtypes. RT-LAMP targeting the universal M gene was designed to screen for the presence of AIV and RT-LAMP assays targeting H5-HA and H9-HA were designed to discriminate between the H5 and H9 subtypes. All three RT-LAMP assays showed specific amplification results without nonspecific reactions. In terms of sensitivity, the detection limits of our RT-LAMP assays were 100 to 1,000 RNA copies per reaction, which were 10 times more sensitive than the detection limits of the reference reverse-transcription polymerase chain reaction (RT-PCR) (1,000 to 10,000 RNA copies per reaction). The reaction time of our RT-LAMP assays was less than 30 min, which was approximately four times quicker than that of conventional RT-PCR. Altogether, these assays successfully detected the existence of AIV and discriminated between the H5 or H9 subtypes with higher sensitivity and less time than the conventional RT-PCR assay.

• 한국컴퓨터정보학회논문지
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• 제20권9호
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• pp.147-153
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• 2015

This Study is to test the causal relationships among university student's stress, personal relationships and psychological anxiety, and then suggest some theoretical and policy implications basing on the analytical results. For the purpose of this study, survey targeting on 416 university students was conducted. The analytical results of this study showed that psychological anxiety moderates the relation between the stress, personal relationship and smart phone addiction. This study suggested some implications basing on this analytical results.

• 대한약침학회지
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• 제26권4호
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• pp.366-372
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• 2023

Objectives: We aimed to evaluate the genotoxicity of a recently developed no-pain pharmacopuncture (NPP) targeting muscle relaxation and analgesia using the micronucleus test. Methods: To evaluate the potential of NPP extracts to induce micronuclei in rat bone marrow cells, a micronucleus test was performed using male Sprague-Dawley rats. The test substance NPP was administered intramuscularly at concentrations of 0.25, 0.5, and 1 mL/animal. Saline was used as the negative control and cyclophosphamide as the positive control. Results: No NPP treatment-related deaths or abnormal changes in general appearance were observed at any dose level during the experimental period. No statistically significant differences in body weight were observed in any of the NPP dose groups compared to the saline negative control group. NPP did not cause a significant increase in the incidence of micronucleated polychromatic erythrocytes (PCEs) and PCEs or in the ratio of PCE-to-total erythrocytes. Conclusion: The NPP extract did not exhibit genotoxic in Sprague-Dawley rat bone marrow cells under the conditions of this study. Further toxicity studies of the NPP extract are required.

• 한국정보시스템학회지:정보시스템연구
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• 제26권1호
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• pp.143-169
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• 2017

Purpose With the increase of the elderly population, the growth of its information system related market is increasing. However, empirical studies for the usage of elderly people's smartphones are still limited. This study aims to investigate the factors that affect the seniors' smartphone usage behaviors. Design/Methodology/Approach First, a preliminary study was conducted with targeting seniors who have visited Gwangju Senior Technology Center(GSTC) in order to understand the factors that affect the intention of utilizing smartphones. Next, based on the preliminary study results and literature reviews including the UTAUT model, performance expectancy, effort expectancy and social influence were considered for the major factors affecting the usage of elderly people's smartphones. Also, the moderate effects of personal innovativeness and usage function were discussed. A questionnaire survey of 181 seniors visiting GSTC was analyzed to empirically test the research model. Findings By the empirical tests, the performance expectancy and the effort expectancy of the seniors had significant impacts on their smartphone usage behavior. While the moderating effect of personal innovativeness was not significant, the moderating effect of the usage function was significant in the relationship between performance expectancy and the smartphone usage behavior. The results of this study are expected to provide guidelines to excavate the technical development and the niche market targeting the elderly people.

• Asian Pacific Journal of Cancer Prevention
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• 제17권3호
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• pp.1571-1576
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• 2016

Alteration of the acetylation status of chromatin and other non-histone proteins by HDAC inhibitors has evolved as an excellent epigenetic strategy in treatment of cancers. The present study was sought to identify compounds with positive pharmacological profiles targeting HDAC1. Analogues of Vorinostat synthesized by Cai et al, 2015 formed the test compounds for the present pharmacological evaluation. Hydroxamte analogue 6H showed superior pharmacological profile in comparison to all the compounds in the analogue dataset owing to its better electrostatic interactions and hydrogen bonding patterns. In order to identify compounds with even better high affinity and pharmacological profile than 6H and Vorinostat, virtual screening was performed. A total of 83 compounds similar to Vorinostat and 154 compounds akin to analogue 6H were retrieved. SCHEMBL15675695 (PubCid: 15739209) and AKOS019005527 (PubCid: 80442147) similar to Vorinostat and 6H, were the best docked compounds among the virtually screened compounds. However, in spite of having good affinity, none of the virtually screened compounds had better affinity than that of 6H. In addition SCHEMBL15675695 was predicted to be a carcinogen while AKOS019005527 is Ames toxic. From, our extensive analysis involving binding affinity analysis, ADMET properties predictions and pharmacophoric mappings, we report Vorinostat hydroxamate analogue 6H to be a potential candidate for HDAC inhibition in treatment of cancers through an epigenetic strategy.

• Asian Pacific Journal of Cancer Prevention
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• 제15권15호
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• pp.6243-6246
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• 2014

To investigate the role of miR-1297 and the tumor suppressor gene PTEN in cell proliferation of testicular germ cell tumors (TGCT). MTT assays were used to test the effect of miR-1297 on proliferation of the NCCIT testicular germ cell tumor cell line. In NCCIT cells, the expression of PTEN was assessed by Western blotting further. In order to confirm target association between miR-1297 and 3'-UTR of PTEN, a luciferase reporter activity assay was employed. Moreover, roles of PTEN in proliferation of NCCIT cells were evaluated by transfection of PTEN siRNA. Proliferation of NCCIT cells was promoted by miR-1297 in a concentration-dependent manner. In addition, miR-1297 could bind to the 3'-UTR of PTEN based on luciferase reporter activity assay, and reduced expression of PTEN at protein level was found. Proliferation of NCCIT cells was significantly enhanced after knockdown of PTEN by siRNA. miR-1297 as a potential oncogene could induce cell proliferation by targeting PTEN in NCCIT cells.

• 폴리머
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• 제33권6호
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• pp.515-519
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• 2009

생분해성 고분자인 poly((R)-3-hydroxy butyrate)와 poly(ethylene glycol)을 결합시켜 양친성 이중블록 공중합체를 형성하었고, 표적인자인 folate를 수식하여 특정 암세포에 표적화하도록 설계하였다. 이 공중합체는 수용액상에서 미셀을 이루며, DLS로 측정한 결과, 125~156 nm의 크기였고, 동결건조하여 SEM으로 관찰한 결과 구형임을 확인하였다. 여기에 소수성 약물인 griseofulvin을 사용하여, 35~56%의 봉입률을 나타내었다. 약물은 in vitro상에서 24시간 동안 지속적으로 방출되었다. 세포생존율을 측정하여, folate가 수식된 입자가 그렇지 않은 입자보다 약 10% 더 낮은 세포생존율을 보임으로써 표적지향 효과가 있다는 것을 알 수 있었다.

• 한국의류학회지
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• 제25권6호
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• pp.1112-1121
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• 2001

The purpose of the study was to investigate and make comparison of brand images of men's wear brands and women's wear brands targeting middle-aged people or older people. Thus, the study could be an exploratory study to search apparel niche-market for middle-aged or older consumers. The questionnaire consisted of questions on 28 brand image. The subjects were to evaluate their brand images and rate on 5 point Likert-type scales of 28 adjectives. Most of the subjects were designers or merchandisers who worked for men'wear brands or women's wear brands. The SPSS/WIN Program was used to calculate frequency, t-test, and cluster analysis. The results from the study were as follow : 1) The common images which men's wear brands and women's wear brands strongly pursued were prestigious, luxurious. 2) Each 37 men's wear brands and 36 women's wear brands were clustered into one groups by brand images. The common images included in first clusters of men's wear and woman's wear brands consisted of prestigious. luxurious, formal, comfort, and conservative. This market needs to be more segmented by differentiated brand images.

• 조명전기설비학회논문지
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• 제27권9호
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• pp.23-35
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• 2013

In this study, we collected the emotion vocabulary novel related to the LED system light emotion and the existing terms through previous research, targeting professionals and KJ method, we selected emotion term of 35 kinds. Targeting this east Foreigner, we compared the emotion evaluation by changing the lighting elements in accordance office, meeting room, lounge, the OA room different behavior patterns. The results, showing the difference between the results of emotion existing research generally derived factors three axes "future, functionality" See, "stability", "activity". As a result of the comparison of the emotion of the East and the West, the Oriental, functional aspects of the LED system light of space office, meeting room, lounge is drawn most, On the other hand, Westerners, come up, stable surface shows the difference in accidentally. Based on these results, the future, and tries to utilized to evaluate the emotion reaction of the illumination elements each Test-Bed actual.

• 한국학교ㆍ지역보건교육학회지
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• 제16권2호
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• pp.101-114
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• 2015

Objectives: Find out how it affects anxiety situations and self-esteem targeting the critical period of development of self-esteem in the lower grades in elementary school based on laughter therapy in having a positive influence on physical health promotion and positive emotional cultivation. And to verify the effects of laughter activity was to provide preliminary data on mental health education and guidance of school life. Methods: By targeting Ulsan Y elementary school, second grade 7 class, 191 students conducted test self-esteem and anxiety. Homogeneous group of experimental and control groups were selected in each one class. Classified as experimental group carried out laughter activity programs and control group did not carried out. Also the separation of the application program before and after, then it were performed preliminary-post-further as same checklist. Program coverage period is 12 weeks and 30 minutes per session was conducted twice a week. Differences between the experimental group and the comparison group has conducted the independent samples t-test, and if the difference between the experimental group and the comparison group were verified by performing a variance analysis(Oneway ANOVA) and post multiple comparisons. Results: Analysis of the experimental group decreased anxiety in the post-test than the pre-test as a whole, in particular, sub-areas of concern, shame and shyness was shown to be effective, and decision of the difficulty was not significant by insignificant reduction. In addition, self-esteem was increased in overall score. The score of collective self-esteem and School life self-esteem were more increased, and domestic self-esteem was not significant by slight increases. In contrast, the comparison group showed no significant difference in all areas of anxiety and self-esteem of pre-test and post-test. Conclusions: In experiments on self-esteem and anxiety of lower grade in elementary school through laughter activity program, comparison group it did not show an effect by accident, and experimental group showed meaningful effects that self-esteem was increased and anxiety was reduced. Thus, laughter activity can be seen that improving self-esteem, anxiety reduction and etc, is very beneficial to mental health.