Polymer(Korea) (폴리머)

The Polymer Society of Korea (한국고분자학회)
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Targeted Drug Delivery Carriers Using Folate Conjugated Poly((R)-3-hydroxy butyrate)-Poly(ethylene glycol) Nanoparticles

Folate가 수식된 Poly((R)-3-hydroxy butyrate)-Poly(ethylene glycol) 나노입자를 이용한 표적지향형 약물전달체

  • Kwon, Seung-Ho (Department of Applied Chemistry & Biological Engineering, Chungnam National University) ;
  • Kim, Young-Jin (Department of Applied Chemistry & Biological Engineering, Chungnam National University)
  • 권승호 (충남대학교 공과대학 바이오응용화학과) ;
  • 김영진 (충남대학교 공과대학 바이오응용화학과)
  • Published : 2009.11.25
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Abstract

Biodegradable poly((R)-3-hydroxy butyrate) and poly(ethylene glycol) was conjugated to make amphiphilic di-block copolymer. Folate was conjugated at di-block copolymer to target the cancer cells. Copolymer was ready to form the self-assembled micelle whose size was 125~156 nm in aqueous solution. Griseofulvin as a hydrophobic drug was loaded in nanoparticles. Their loading efficiencies were 35~56%. Hydrophobic drug was continuously released for 24 h. Cell viability test showed that folate attached particles were 10% more efficient than the particles without targeting ligands.

생분해성 고분자인 poly((R)-3-hydroxy butyrate)와 poly(ethylene glycol)을 결합시켜 양친성 이중블록 공중합체를 형성하었고, 표적인자인 folate를 수식하여 특정 암세포에 표적화하도록 설계하였다. 이 공중합체는 수용액상에서 미셀을 이루며, DLS로 측정한 결과, 125~156 nm의 크기였고, 동결건조하여 SEM으로 관찰한 결과 구형임을 확인하였다. 여기에 소수성 약물인 griseofulvin을 사용하여, 35~56%의 봉입률을 나타내었다. 약물은 in vitro상에서 24시간 동안 지속적으로 방출되었다. 세포생존율을 측정하여, folate가 수식된 입자가 그렇지 않은 입자보다 약 10% 더 낮은 세포생존율을 보임으로써 표적지향 효과가 있다는 것을 알 수 있었다.

Keywords

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