• 한국건축시공학회지
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• 제9권3호
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• pp.95-101
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• 2009

Constructing large-scale mat foundation mass concrete is increasing for the stability of building structure, because a lot of high rise building are being built in order to make full use of limited space. However, It is of increasing concerns that because limited placing equipments, available job-site and systems for mass concete placement in construction field do not allow to place great quantity of concrete at the same time in large scale mat foundation, consistency between placement lift can not be secured. And also, it is likely to crack due to stress caused by the difference of hydration heat generation time. To find out the solution against above problems, this study is to reconfirm the performance of normal concrete designed by mix proportion and super retarding concrete. The Fundamental test shows what happens if low heat proportioning and control method of setting time are applied at the job-site of newly constructed high rise building. The test result show that slump flow of concrete has been somewhat increased as the target retarding time gets longer, while the air content has been slightly decreased but this is no great difference from normal concrete. The setting time shows to be retarded as target retarding time gets longer, the range of retarding time increases. It is necessary to increase the amount of mix of super retarding agent in the proportion ration by setting curing temperature high since outdoor curing is about 6 hours faster than standard curing, which means the temperature of the concrete will be higher than the temperature of the surrounding environment, due to its high hydration heat when applying in a construction site. The compressive strength of super retarding concrete appears to be lower than normal concrete due to the retarding action in the early stage. However, as the time goes by, the compressive strength gets higher, and by the 28th day the strength becomes the same or higher than normal concrete.

• BMB Reports
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• 제43권6호
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• pp.419-426
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• 2010

Aeromonas hydrophila is a bacterial pathogen that infects a large number of eukaryotes, including humans. The UDP-galactose 4'-epimerase (GalE) catalyzes interconversion of UDP-galactose to UDP-glucose and plays a key role in lipopolysaccharide biosynthesis. This makes it an important virulence determinant, and therefore a potential drug target. Our earlier studies revealed that unlike other GalEs, GalE of A. hydrophila exists as a monomer. This uniqueness necessitated elucidation of its structure and active site. Chemical modification of the 6xHis-rGalE demonstrated the role of histidine residue in catalysis and that it did not constitute the substrate binding pocket. Loss of the 6xHis-rGalE activity and coenzyme fluorescence with thiol modifying reagents established the role of two distinct vicinal thiols in catalysis. Chemical modification studies revealed arginine to be essential for catalysis. Site-directed mutagenesis indicated Tyr149 and Lys153 to be involved in catalysis. Use of glycerol as a cosolvent enhanced the GalE thermostability significantly.

• Journal of Forest and Environmental Science
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• 제34권4호
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• pp.275-283
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• 2018

This study created the running water (Bandibooliwon) and lentic water (Sooryunwon) artificial habitats of Luciola lateralis in Hwadamsup, Gwangju-si, Gyunggi-do from April 2014 to February 2016. Through various habitat types, metapopulation was formed, and more sound population was induced to be maintained via interactions between habitats. We investigated coenosite at the habitat creation-target site, and 20 species in the lentic water site and 38 species in running water site were found. After releasing L. lateralis, we checked the status of settlement of L. lateralis larvae through traps and macroscopy, controlled the flow velocity of the habitats in line with the life cycle of L. lateralis (hatching, larva period, landing, pupation, copulation, and oviposition), and shaped gentle slope at water side, and planted moss. We artificially supplied melanian snails (Semisulcospira sp.), the food source, in the running water site and induced stable feeding. Also, We created artificial insectary, bred larvae stably in winter, and secured firefly population for a festival. Through night time observation, the time most suitable for a festival was confirmed to be from 20:30 to 22:00.

• 한국지반공학회논문집
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• 제29권3호
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• pp.41-50
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• 2013

지반 해석 결과에는 항상 불확실성이 존재하며, 이러한 불확실성은 주로 지반 구조의 불확실한 평가에 기인한다. 이러한 지반 구조의 불확실성에 따른 지반 해석 결과의 불확실성은 몬테카를로 시뮬레이션을 사용한 확률론적 해석에 의해 고려될 수 있다. 몬테카를로 시뮬레이션을 사용한 확률론적 지반 해석의 수행을 위해서는 대상 지반에서 존재 가능한 다양한 지반 구조의 생성이 필요하다. 본 연구에서는 확률론적 지반 해석을 위한 대상 지반에서 존재 가능한 다양한 지반 구조 생성 방법을 제안하였다. 제안된 방법은 대상 지반에서 획득된 지반 조사 결과를 바탕으로 하모닉 웨이브릿 변환을 사용하여 지반 구조를 평가한 후, 이로부터 대상 지반에 존재 가능한 다양한 지반 구조를 생성할 수 있다. 제안된 방법은 실제 현장 조사 결과들에 적용되었으며 이를 통해 제안된 방법의 현장 적용성을 확인할 수 있었다.

• KIEAE Journal
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• 제6권4호
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• pp.3-10
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• 2006

To preserve scenic-view of Gwanak Mountain from the urban development, the viewshed component ratio and the scenic-view preference are investigated and analyzed by selecting view points at Nangokro. After 16 candidate view points are selected from the site, sight component ratio is measured through visual structure analysis and preference are measured by using the Likert scale. In addition, factor analysis, and regression analysis were conducted to examine the relationship between influencing factors and preference. As a result of the regression analysis of the 9 factors to influence the scenic-view preference, two factors such as degree of natural landscape and degree of arrangement around the site are chosen to influence the scenic-view preference. Through the preference factor analysis, two preference factors are selected as follows; 1) The factor of the landscape formation - Existence and nonexistence of the scenic-view conservation, degree of the landscape opening, and degree of natural landscape, degree of the landscape originality, and degree of the landscape sequence, degree of the landscape intimacy 2) The factor surrounding the landscape - Degree of arrangement around the site, degree of the landscape complexity, and atmosphere around the site.

• 환경영향평가
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• 제22권1호
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• pp.79-87
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• 2013

The purpose of this study is to establish PM-10 management manual for developing large scale sites by assessing the status of PM-10 reduction at ongoing large scale development sites. After analyzing the meteorological conditions and air quality characteristics of Sihwa MTV development site, ISCST3 (Industrial Source Complex Short Term Model 3) was implemented to predict PM-10 generation. The outcomes of ISCST3 modelling were utilized for verification of site survey data. As a result of applying air pollution modeling, the diffusion rate of PM-10 decreases according as the wind speed decreases. And the emission rate of PM-10 increase is linear to the concentration of PM-10. The reduction target of PM-10 can be derived quantitatively from the difference between the forecasted emission rate and the permissible emission limit of PM-10. The assessment of PM-10 characteristics which is deduced from ISCST3 and site survey can be practically applied to accomplish environmentally acceptable air quality manual for large scale development sites.

• 한국건축시공학회지
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• 제3권3호
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• pp.107-112
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• 2003

This study is to investigate the properties of concrete with crushed sand on site and to propose a quality guideline for its use as artificial sand and concrete. From our experimental result in laboratory and site, we found that demand water of concrete with crushed sand for target slump increased by 18kg/m3 compared to mixed sand and l8kg/m3 compared to sea sand respectively. The compressive strength increased by around 3∼6% when compared to concrete with sea sand. Accordingly, our study showed that the combined sand mixed with sea sand would be desirable to obtain workability and strength of concrete including dry shrinkage and bleeding test. Furthermore, the optimal replacement percentage of crushed sand was 50% with sea sand. As such, crushed sand would be sufficient as fine aggregate for concrete in terms of economic efficiency and quality. Crushed sand, on the other hand can only be used as fine aggregate when VFS(Very Fine Sand) is below 3.5 percentage of weight of sand and particle shape is above 55 percentage. Also, the particle shape and microsand passing NO.200 sieve should continually be improved to increase workability of concrete on site.

• Geomechanics and Engineering
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• 제34권1호
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• pp.29-41
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• 2023

The 3D geospatial modeling of geotechnical information can aid in understanding the geotechnical characteristic values of the continuous subsurface at construction sites. In this study, a geostatistical optimization model for the three-dimensional (3D) mapping of subsurface stratification and the SPT-N value based on a trial-and-error rule was developed and applied to a dam emergency spillway site in South Korea. Geospatial database development for a geotechnical investigation, reconstitution of the target grid volume, and detection of outliers in the borehole dataset were implemented prior to the 3D modeling. For the site-specific subsurface stratification of the engineering geo-layer, we developed an integration method for the borehole and geophysical survey datasets based on the geostatistical optimization procedure of ordinary kriging and sequential Gaussian simulation (SGS) by comparing their cross-validation-based prediction residuals. We also developed an optimization technique based on SGS for estimating the 3D geometry of the SPT-N value. This method involves quantitatively testing the reliability of SGS and selecting the realizations with a high estimation accuracy. Boring tests were performed for validation, and the proposed method yielded more accurate prediction results and reproduced the spatial distribution of geotechnical information more effectively than the conventional geostatistical approach.

• 한국안전학회지
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• 제35권1호
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• pp.45-52
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• 2020

Construction industry has a relatively higher industrial accident rate than other domestic industries. Thus, to reduce the accident rate, researches on the methodology of worker's safety education combined with new technologies like IT technology have increased. In the light of workers' safety information provision, this study develops a VR(Virtual Reality)-based construction site using the BIM(Building Information Modeling) data. The target structures and geographical features are included in the VR-based construction site where the construction machinery model and worker model are also created using a game engine. For highly effective provision of safety information, video clips with suitable captions corresponding to working processes were made with proper screen directing. They should be appropriately connected to correct worker's operations to improve the work commitment level, sense of reality and inducement of interest. From this scenario, the 3D VR-based construction site, which can be experienced through a VR equipment, was created and in the same platform, the safety information was provided by the video clip combined with the suitable captions. Although the real construction site involves various requirements depending on field situation and the expertness and experience of workers are not consistent, the developed safety information provision based on the VR construction site is expected to effectively reduce the incomplete factors leading to construction accidents by improving the worker's perception of workplace safety.

• The Plant Pathology Journal
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• 제34권3호
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• pp.191-198
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• 2018

Fast and accurate diagnosis is needed to eradicate and manage economically important and invasive diseases like fire blight. Loop-mediated isothermal amplification (LAMP) is known as the best on-site diagnostic, because it is fast, highly specific to a target, and less sensitive to inhibitors in samples. In this study, LAMP assay that gives more consistent results for on-site diagnosis of fire blight than the previous developed LAMP assays was developed. Primers for new LAMP assay (named as DS-LAMP) were designed from a histidine-tRNA ligase gene (EAMY_RS32025) of E. amylovora CFBP1430 genome. The DS-LAMP amplified DNA (positive detection) only from genomic DNA of E. amylovora strains, not from either E. pyrifoliae (causing black shoot blight) or from Pseudomonas syringae pv. syringae (causing shoot blight on apple trees). The detection limit of DS-LAMP was 10 cells per LAMP reaction, equivalent to $10^4$ cells per ml of the sample extract. DS-LAMP successfully diagnosed the pathogens on four fire-blight infected apple and pear orchards. In addition, it could distinguish black shoot blight from fire blight. The $B{\ddot{u}}hlmann$-LAMP, developed previously for on-site diagnosis of fire blight, did not give consistent results for specificity to E. amylovora and on-site diagnosis; it gave positive reactions to three strains of E. pyrifoliae and two strains of P. syringae pv. syringae. It also, gave positive reactions to some healthy sample extracts. DS-LAMP, developed in this study, would give more accurate on-site diagnosis of fire blight, especially in the Republic of Korea, where fire blight and black shoot blight coexist.