• Archives of Pharmacal Research
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• 제29권12호
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• pp.1164-1170
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• 2006

A triptolide-lysozyme (TP-LZM) conjugate was synthesized to achieve renal specific delivery and to reduce the side effects of triptolide. Triptolide was coupled to lysozyme through succinic via an ester bond with an average coupling degree of 1 mol triptolide per 1 mol lysozyme. The lysozyme can specifically accumulate in the proximal tubular cells of the kidney, making it a potential carrier for targeting drugs to the kidney. The structure of triptolide succinate (TPS) was confirmed by IR, $^{1}H-NMR$, MS and UV. The concentrations of triptolide in various samples were determined by reversed-phase high-performance liquid chromatography (HPLC). In this study, the physicochemical and stability profiles of TP-LZM under various conditions were investgated the stability and releasing profiles of triptolide-lysozyme (TP-LZM) under various conditions. In vitro release trails showed triptolide-lysozyme was relatively stable in plasma (less than 30% of free triptolide released) and could release triptolide quickly in lysosome (more than 80% of free triptolide released) at $37^{\circ}C$ for 24 h. In addition, the biological activities of the conjugate on normal rat kidney proximal tubular cells (NRK52E) were also tested. The conjugate can effectively reduce NO production in the medium of NRK52E induced by lipopolysaccharide (LPS) but with much lower toxicity. These studies suggest the possibility to promote curative effect and reduce its extra-renal toxicity of triptolide by TP-LZM conjugate.

• 환경영향평가
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• 제25권6호
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• pp.369-383
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• 2016

본 연구에서는 강원도 평창군 대관령면에 위치한 산지초지와 초지의 말단에 조성한 식생여과대(Vegetation Buffer Strip, VBS)에 분포형 유역모델 CAMEL을 적용하였다. 그리고 이를 통해 확보한 매개변수를 초지와 식생여과대로 구분한 다수의 시험격자에 적용하여 다양한 인자들에 의한 식생여과대 오염부하 저감효과를 평가하였다. 시험격자를 이용한 시나리오 모의결과, 식생여과대에서 강우의 직접유출량은 식생여과대 폭이 넓어질수록 감소하는 것으로 나타났으며, 전반적으로 경사가 높을수록 오염물질의 저감효율은 낮아지는 것으로 나타났다. 유사의 경우, 식생여과대 폭에 따른 저감효율은 전반적으로 큰 차이를 보이지 않았다. TOC와 TN의 경우, 시험격자의 경사가 $10^{\circ}$, $20^{\circ}$일 때, 식생여과대 폭에 따른 저감효율은 큰 차이를 보이지 않았으나, 경사가 $30^{\circ}$일 때 식생여과대 폭이 좁을수록 높은 저감효율을 보였다. 반면, TP의 경우, 양토(Loam)에서의 저감효율은 전반적으로 높지만, 사양토(Sandy loam)에서는 경사가 $20^{\circ}$, $30^{\circ}$일 때 식생여과대 폭이 넓을수록 높은 저감효율을 보이는 것으로 분석되었다. 이는 TOC와 TN의 모의결과와는 상반되는 결과로 대부분 입자성 물질로 존재하는 인의 경우 지표면에서의 포착현상으로 인해 길이에 따라 비례적으로 저감효율이 높아지는 경향을 보이는 것으로 사료된다. 본 연구에서 구축한 모델은 향후 비점오염물질의 유출을 효과적으로 저감할 수 있는 식생여과대 조성을 위한 기준 등을 제안하는데 효과적으로 사용될 수 있을 것으로 기대된다.

• 생물환경조절학회지
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• 제27권1호
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• pp.71-79
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• 2018

본 연구는 우량묘 생산을 위해 신개발된 인공배지와 육묘용 배지의 이화학적 특성을 비교하고 적정 관수 간격을 구명하기 위해 수행되었다. 5종의 기존 인공배지(유기배지인 coir, 혼합배지인 Tosilee와 Q plug, 그리고 무기배지인 LC와 rockwool)와 4종의 신개발 배지(혼합배지인 TP-S1, 그리고 무기배지인 PU 14-S1, PU-7B, PU 15-S1)를 이용하여 토마토(Solanum lycopersicum L.) '예광'을 육묘하였으며, 14일간 1일(14회), 2일(7회) 그리고 3일(5회) 간격으로 관수 처리하였다. pH는 PU 15-S1 배지에서 유의성 있게 가장 높았으며, 모든 배지에서 pH 5.17-6.90의 범위였다. EC는 Q plug 배지에서 가장 높게 나타났다. 초기발아율은 PU 15-S1 배지에서 가장 우수하였다. 최종발아율과 평균발아수는 PU 14-S1 배지를 제외하고는 모든 배지에서 유의적인 차이가 없었다. 파종 후 15일째 토마토 묘의 생육은 Q plug 배지에서 우수하였다. 파종 후 29일 째 묘의 생육 또한 Q plug 배지에서 유의적으로 우수하게 나타났고, 다음으로 rockwool과 PU-7B 배지에서 우수하였다. 또한 생육은 1일 간격 처리에서 가장 우수한 경향을 보였다. 결과적으로 본 실험에서 토마토 육묘 시 인공배지의 적용가능성을 확인하였으며, 묘의 생육은 Q plug 배지에서 가장 우수하였다. 신개발된 PU-7B 인공배지에 양분을 첨가하고 1일 간격으로 관수한다면 Q plug 배지의 결과와 같이 우수한 토마토 묘를 생산할 수 있을 것으로 판단된다.

• Journal of Microbiology and Biotechnology
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• 제20권12호
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• pp.1696-1701
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• 2010

The ${\alpha}$-amylases activity was improved by random mutagenesis and screening. A region comprising residues from the position 34-281 was randomly mutated in B. licheniformis ${\alpha}$-amylase (AmyL), and the library with mutations ranging from low, medium, and high frequencies was generated. The library was screened using an effective liquid-phase screening method to isolate mutants with an altered pH profile. The sequencing of improved variants indicated 2-5 amino acid changes. Among them, mutant TP8H5 showed an altered pH profile as compared with that of wild type. The sequencing of variant TP8H5 indicated 2 amino acid changes, Ile157Ser and Trp193Arg, which were located in the solvent accessible flexible loop region in domain B.

• ALGAE
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• 제32권4호
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• pp.275-284
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• 2017

Microcystis aeruginosa causes harmful algal blooms in the Nakdong River of Korea. We studied the effect of different concentrations and ratios of ammonium ($NH_4{^+}$), nitrate ($NO_3{^-}$), and phosphate ($PO{_4}^{3-}$) on growth of this species in BG-11 medium: each nutrient alone, $NO_3{^-}:NH_4{^+}$ ratio, the N : P ratio with fixed total N (TN), and the N : P ratio with fixed total P (TP). The single nutrient experiments indicated that M. aeruginosa had the highest growth rate at $NH_4{^+}$ and $NO_3{^-}$ concentrations of $500{\mu}M$, and at a $PO{_4}^{3-}$ concentration of $5{\mu}M$. The $NO_3{^-}:NH_4{^+}$ ratio experiments showed that M. aeruginosa had the highest growth rate at a ratio of 1 : 1 when TN was $100{\mu}M$ and $250{\mu}M$, and the lowest growth rate at a ratio of 1 : 1 when the TN was $500{\mu}M$. The N : P ratio with fixed TN experiments indicated that M. aeruginosa had the highest growth rates at 50 : 1, 20 : 1, and 100 : 1 ratios when the TN was 100, 250, and $500{\mu}M$, respectively. In contrast, the N : P ratio with fixed TP experiments showed that M. aeruginosa had the highest growth rates at 200 : 1 ratio at all tested TP concentrations. In conclusion, our results imply that the $NO_3{^-}:NH_4{^+}$ ratio and the $PO{_4}^{3-}$ concentration affect the early stage of growth of M. aeruginosa. In particular, our results suggest that the maximum growth of M. aeruginosa is not simply affected by the $NO_3{^-}:NH_4{^+}$ ratio and the N : P ratio, but is determined by the TN concentration if a certain minimum $PO{_4}^{3-}$ concentration is present.

• 한국물환경학회지
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• 제20권6호
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• pp.563-570
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• 2004

Phased isolation intrachannel clarifier ditch process developed in this study is an enhanced biological nutrient removal process employing two ditches with intrachannel clarifiers. Bench-scale phased isolation ditch process was used to evaluate the system performance on municipal wastewater and detailed assessment of internal behavior in a ditch and each reactions. When the system was operated at the HRTs of 6~12hours, SRTs of 9~31 days, and cycle times of 4hours, the system showed removals of BOD, TN, and TP as high as 88~97%, 73~78%, and 65~90%, respectively. The internal behavior were well matched on each reactions such as nitrification, denitrification, and phosphorus release and uptake. As the SRT became longer, TN removal increased gradually, whereas TP removal decreased contrarily. However, the system was capable of producing an effluent TP concentration 1mg/L or less even at longer SRTs except the case of solids discharge by malfunction of intra-clarifier occurred by its geometrical limit. The system performance slightly decreased by hydraulic shock loading(increasing of influent flowrate and decreasing of system HRT). However, the higher system performance could be achieved again after four cycles. Thus, the system reliability could be successfully achieved short-term hydraulic shock loading that occurred in medium- and small-sized wastewater treatment plants suffering fluctuation of influent quality and flowrate during wet season.

• 한국식품영양학회지
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• 제24권1호
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• pp.117-123
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• 2011

This study was conducted to establish process conditions for plant-originated lactic acid production using a mixed culture of plant originated lactic acid bacteria, Lactobacillus sakei B2-16, and Lactobacillus plantarum P23, which were isolated from kimchi, and Bacillus subtilis, which was TP6 isolated from Denjang. Soybean medium was pretreated for 10 minutes at $110^{\circ}C$ and hydrolyzed with 0.2%(w/v) cellulase at $55\sim60^{\circ}C$ for at least 2 hrs. The quality of the final fermentation product was influenced by the inoculation ratio of the Lactobacillus sakei B2-16, Lactobacillus plantarum P23, and Bacillus subtilis TP6. The optimum microorganism inoculation ratio was 1:0.7:0.3, Lactobacillus sakei B2-16: Lactobacillus plantarum P23: Bacillus subtilis TP6, respectively. The sensory characteristics of the product were a refreshing sourness and a soft flavor.

• 대한환경공학회지
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• 제31권9호
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• pp.755-764
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• 2009

시화호 인공습지내 표층퇴적토에 의한 오염물질 제거효율을 평가하기 위하여 표층퇴적물내 물질함량과 퇴적토에 의한 오염물질의 용출여부를 조사하였다. 조사기간 동안 표층퇴적물의 COD, TOC, IL, TN, TP 함량은 각각 4.1~47.7 mg/g, 0.29~2.81%, 1.88~8.15%, 0.03~0.35%, 362~1,150 ${\mu}g$/g의 범위로 조사지점과 시기에 따라 차이를 보였다. 유기물과 TN 함량은 봄에 높고 시간이 지날수록 감소하는 경향을 보인 반면 TP 함량은 유의적인 차이를 보이지 않았다(3월${\geq}$5월${\geq}$7월${\geq}$9월, p=0.003 for COD, p=0.001 for TOC, p=0.017 for IL, p=0.015 for TN). 표층퇴적토의 중금속 함량은 As 3.5~13.9 ${\mu}g$/g, Cd 0.08~0.38 ${\mu}g$/g, Cr 51.8~107.0 ${\mu}g$/g, Cu 16.4~81.8 ${\mu}g$/g, Pb 26.8~81.8 ${\mu}g$/g, Zn 85~559 ${\mu}g$/g의 범위로 항목에 따라 상이한 시공간적인 분포를 보였다. 퇴적물환경기준으로 볼 때 유기물 함량은 대부분 기준 이하의 수준을 보인 반면 TN과 TP는 "중간오염" 또는 "심한 오염" 수준을 보였다. 그리고 중금속 함량은 Cd과 Pb을 제외한 모든 항목에서 퇴적물 환경기준으로 초과하는 것으로 나타났다. 퇴적토의 용출실험 결과로부터 TN, Pb, Zn은 퇴적토에 의해 제거되는 반면 TP, Cd, Cu는 퇴적토로부터 용출되는 경향을 보였다. 그러므로 시화호 인공습지에서 표층퇴적토의 수질정화기능을 향상시킬 수 있는 방안수립이 필요하다.

• The Journal of Asian Finance, Economics and Business
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• 제7권7호
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• pp.209-217
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• 2020

Taxes are levied in almost every country, primarily to raise revenue for government expenditures. This study explores factors influencing tax compliance of small- and medium-sized enterprises (SMEs) in Vietnam. Data from 376 SMEs, who are business taxpayers, were collected through a researcher-administered questionnaire survey method. The results indicate that six groups of factors have significant impacts on tax compliance among Vietnamese SMEs. These groups include: Business characteristics (BC), Characteristics of accounting practices within organization (AP), Awareness of tax obligations (TO), Tax policy (TP), View on tax compliance (TC), and Probability of tax examination on taxpayer compliance (TE). Multivariate analysis was adopted; Cronbach's alpha coefficients were calculated, then, Exploratory Factor Analysis (EFA) was used. The findings show that, among these six factors, the most influential is Characteristics of accounting practices (AP). Thus, it is recommended that tax agencies should help SMEs improve their accounting skills and increase their knowledge by organizing training workshops and short courses on taxation. SMEs also need to have an adequate accounting system in accordance with principles and standards prescribed by the Tax Law. It is expected that this study can provide important insights and understandings to policy-makers, practitioners, academicians and other regulatory authorities in tax policy formulations.

• Journal of Microbiology and Biotechnology
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• 제27권3호
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• pp.514-523
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• 2017

Carbon catabolite repression is a crucial regulation mechanism in microorganisms, but its characteristic in Rhizopus is still unclear. We extracted a carbon regulation gene, cre, that encoded a carbon catabolite repressor protein (CRE) from Rhizopus stolonifer TP-02, and studied the regulation of CRE by real-time qPCR. CRE responded to glucose in a certain range, where it could significantly regulate part of the cellulase genes (eg, bg, and cbh2) without cbh1. In the comparison of the response of cre and four cellulase genes to carboxymethylcellulose sodium and a simple carbon source (lactose), the effect of CRE was only related to the concentration of reducing sugars. By regulating the reducing sugars to range from 0.4% to 0.6%, a glucose-containing medium with lactose as the inducer could effectively induce cellulases without the repression of CRE. This regulation method could potentially reduce the cost of enzymes produced in industries and provide a possible solution to achieve the largescale synthesis of cellulases.