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http://dx.doi.org/10.4014/jmb.1608.08048

Production of Cellulases by Rhizopus stolonifer from Glucose-Containing Media Based on the Regulation of Transcriptional Regulator CRE  

Zhang, Yingying (School of Biotechnology, Jiangnan University)
Tang, Bin (School of Biotechnology, Jiangnan University)
Du, Guocheng (School of Biotechnology, Jiangnan University)
Publication Information
Journal of Microbiology and Biotechnology / v.27, no.3, 2017 , pp. 514-523 More about this Journal
Abstract
Carbon catabolite repression is a crucial regulation mechanism in microorganisms, but its characteristic in Rhizopus is still unclear. We extracted a carbon regulation gene, cre, that encoded a carbon catabolite repressor protein (CRE) from Rhizopus stolonifer TP-02, and studied the regulation of CRE by real-time qPCR. CRE responded to glucose in a certain range, where it could significantly regulate part of the cellulase genes (eg, bg, and cbh2) without cbh1. In the comparison of the response of cre and four cellulase genes to carboxymethylcellulose sodium and a simple carbon source (lactose), the effect of CRE was only related to the concentration of reducing sugars. By regulating the reducing sugars to range from 0.4% to 0.6%, a glucose-containing medium with lactose as the inducer could effectively induce cellulases without the repression of CRE. This regulation method could potentially reduce the cost of enzymes produced in industries and provide a possible solution to achieve the largescale synthesis of cellulases.
Keywords
Cellulases; carbon catabolite repressor; transcriptional regulation; Rhizopus stolonifer;
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1 Ho SN, Hunt HD, Horton RM, Pullen JK, Pease LR. 1989. Site-directed mutagenesis by overlap extension using the polymerase chain resction. Gene 77: 51-59.   DOI
2 Damsteegt EL, McHugh N, Lokman PM. 2016. Storage by lyophilization-resulting RNA quality is tissue dependent. Anal. Biochem. 511: 92-96.   DOI
3 Pearson G, Lago-Leston A, Valente M, Serrao E. 2006. Simple and rapid RNA extraction from freeze dried tissue of brown algae and seagrasses. Eur. J. Phycol. 41: 97-104.   DOI
4 Livak KJ, Schmittgen TD. 2001. Analysis of relative gene expression data using real-time quantitative PCR and the $2^{-{\Delta}{\Delta}CT}$ method. Methods 25: 402-408.   DOI
5 Miller GL, Blum R, Glennon WE, Burton AL. 1960. Measurement of carboxymethylcellulase activity. Anal. Biochem. 1: 127-132.   DOI
6 Cubero B, Scazzocchio C. 1994. Two different, adjacent and divergent zinc finger binding sites are necessary for CREAmediated carbon catabolite repression in the proline gene cluster of Aspergillu nidulans. EMBO J. 13: 407-415.
7 Ruijter GJG, Visser J. 1997. Carbon repression in aspergilli. FEMS Microbiol. Lett. 151: 103-114.   DOI
8 MacPherson S, Larochelle M, Turcotte B. 2006. A fungal family of transcriptional regulators: the zinc cluster proteins. Microbiol. Mol. Biol. Rev. 70: 583-604.   DOI
9 Mach RL, Zeilinger S. 2003. Regulation of gene expression in industrial fungi: Trichoderma. Appl. Microbiol. Biotechnol. 60: 515-522.   DOI
10 Yang F, Gong Y, Liu G, Zhao S, Wang J. 2015. Enhancing cellulase production in thermophilic fungus Myceliophthora thermophila ATCC42464 by RNA interference of cre1 gene expression. J. Microbiol. Biotechnol. 25: 1101-1107.   DOI
11 Berka RM, Grigoriev IV, Otillar R, Salamov A, Grimwood J, Reid I, et al. 2011. Comparative genomic analysis of the thermophilic biomass-degrading fungi Myceliophthora thermophila and Thielavia terrestris. Nat. Biotechnol. 29: 922-929.   DOI
12 Hsieh CC, Cannella D, Jorgensen H, Felby C, Thygesen LG. 2014. Cellulase inhibition by high concentrations of monosaccharides. J. Agric. Food Chem. 62: 3800-3805.   DOI
13 Nogueira KMV, Costa MN, de Paula RG, Mendonca-Natividade FC, Ricci-Azevedo R, Silva RN. 2015. Evidence of cAMP involvement in cellobiohydrolase expression and secretion by Trichoderma reesei in presence of the inducer sophorose. BMC Microbiol. 15: 195.   DOI
14 Kuhad RC, Deswal D, Sharma S, Bhattacharya A, Jain KK, Kaur A, et al. 2016. Revisiting cellulase production and redefining current strategies based on major challenges. Renew. Sustain. Energy Rev. 55: 249-272.   DOI
15 Payne CM, Knott BC, Mayes HB, Hansson H, Himmel ME, Sandgren M, et al. 2015. Fungal cellulases. Chem. Rev. 115: 1308-1448.   DOI
16 Juturu V, Wu JC. 2014. Microbial cellulases: engineering, production and applications. Renew. Sustain. Energy Rev. 33: 188-203.   DOI
17 Hidenoa A, Inouea H, Tsukahara K, Yanoa S, Fang X, Endo T, et al. 2011. Production and characterization of cellulases and hemicellulases by Acremonium cellulolyticus using rice straw subjected to various pretreatments as the carbon source. Enzyme Microb. Technol. 48: 162-168.   DOI
18 Bischof R, Fourtis L, Limbeck A, Gamauf C, Seiboth B, Kubicek CP. 2013. Comparative analysis of the Trichoderma reesei transcriptome during growth on the cellulase inducing substrates wheat straw and lactose. Biotechnol. Biofuels 6: 127.   DOI
19 Chen S, Wayman M. 1992. Novel inducers derived from starch for cellulase production by Trichoderma reesei. Process Biochem. 27: 327-334.   DOI
20 Mandels M, Parrish FW, Reese ET. 1962. Sophorose as an inducer of cellulase in Trichoderma viride. J. Bacteriol. 83: 400-408.
21 Ries L, Belshaw NJ, Ilmen M, Penttila ME, Alapuranen M, Archer DB. 2014. The role of CRE1 in nucleosome positioning within the cbh1 promoter and coding regions of Trichoderma reesei. Appl. Microbiol. Biotechnol. 98: 749-762.   DOI
22 Ronne H. 1995. Glucose repression in fungi. Trends Genet. 11: 12-17.   DOI
23 Drysdale MR, Kolze SE, Kelly JM. 1993. The Aspergillus niger carbon catabolite repressor encoding gene, creA. Gene 130: 241-245.   DOI
24 Lorito M, Mach RL, Sposato P, Strauss J, Peterbauer CK, Kubicek CP. 1996. Mycoparasitic interaction relieves binding of the Cre1 carbon catabolite repressor protein to promoter sequences of the ech42 (endochitinase-encoding) gene in Trichoderma harzianum. Proc. Natl. Acad. Sci. USA 93: 14868-14872.   DOI
25 Strauss J, Mach RL, Zeilinger S, Hartler G, Stoffler G, Wolschek M, et al. 1995. Cre1, the carbon catabolite repressor protein from Trichoderma reesei. FEBS Lett. 376: 103-107.   DOI
26 Mach-Aigner AR, Pucher ME, Steiger MG, Bauer GE, Preis SJ, Mach RL. 2008. Transcriptional regulation of xyr1, encoding the main regulator of the xylanolytic and cellulolytic enzyme system in Hypocrea jecorina. Appl. Environ. Microbiol. 74: 6554-6562.   DOI
27 Lipp M, Brodmann P, Pietsch K, Pauwels J, Anklam E. 1999. IUPAC collaborative trial study of a method to detect genetically modified soy beans & maize in dried powder. J. AOAC Int. 82: 923-928.