• KSBB Journal
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• v.10 no.5
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• pp.499-509
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• 1995

PU.1, a tissue-specific transcription activator, binds to a purine-rich sequence(5'-GAGGAA-3') called PU box. The PU.1 cDNA consists of an open reading frame of 816 nucleotides coding for 272 amino acids. The amino terminal end is highly acidic, while the carboxyl terminal end is highly basic. Transcriptional activation domain is located at the amino terminal end, while DNA binding domain is located at the carboxyl terminal end. Activation of PU.1 transcription factor is supposed to be accomplished by the phosphorylation of serine residue(s). There exist 22 serines in the PU.1. Five(the 41, 45, 132$.$133, and 148th) of the serines(plausible phosphorylation site by casein kinase II), are the primary targets of interest in elucidating the molecular mechanism(s) of the action of the PU.1 gene. In this study, PU.1 cDNA coding for the five serine residues(41th AGC, 45th AGC, 132$.$133th AGC$.$TCA, and 148th TCT), was mutated to alanine codon(41th GCC, 45th GCC, 132$.$133th GCC$.$GCA, and 1481h GCT), respectively, by Splicing-Overlapping-Extension(SOE) using Polymerase Chain Reaction(PCR). And each mutated cDNA fragments was ligated into pBluescript KS＋ digested with HindIII and Xba I, to generate mutant clones named pKKS41A, pRKS45A, pMKS132$.$133A, and pMKS148A. The clones will be informative to study the "Structure and Function" of the immu-nologically important gene, PU.1.

• Corrosion Science and Technology
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• v.10 no.1
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• pp.1-5
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• 2011

The major purpose of the present study is to evaluate the performance of various galvanized (GI) or galvannealed (GA) mild steels and AHSS in stamping applications. Finite Element Analysis (FEA) of selected stamping operations was conducted to estimate the critical pressure boundary conditions that exist in practice. Using this information, laboratory tribotests, e.g. Twist Compression (TCT), Deep Drawing (DDT) and Strip Drawing (SDT) Tests, were developed to evaluate the performance of selected lubricants and die materials/coatings in forming galvanized steels of interest. The sheet materials investigated included mild steels and AHSS (e.g. DP600 GI/GA, DP780 GI/GA, TRIP780 GA and DP980 GI/GA). Experimental results showed that galvanized material resulted in more galling, while galvannealed material showed more powdering and flaking. The surface roughness and chemical composition of galvanized sheet materials affected the severity of galling under the same testing conditions, i.e. lubricants and die materials/coatings. The results of this study helped to determine the critical interface pressure that initiates lubricant failure and galling in stamping selected galvanized sheet materials. Thus, to prevent or postpone the critical interface conditions, the results of this study can be used to select the optimum combination of galvanized sheet, die material, die coating and lubricant for forming structural automotive components.

• Asian Pacific Journal of Cancer Prevention
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• v.15 no.2
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• pp.891-894
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• 2014

This study is aimed to investigate the role of paired boxed gene 1 (PAX1) methylation analysis by methylation-sensitive high-resolution melting (MS-HRM) in the detection of high grade lesions in atypical squamous cells cannot exclude high-grade squamous intraepithelial lesion (ASC-H) and compared its performance with the Hybrid Capture 2 (HC2) human papillomavirus (HPV) test. In our study, 130 cases with a diagnosis of ASC-H from the cervical cytological screening by Thinprep cytologic test (TCT) technique were selected for triage. Their cervical scrapings were collected and evaluated by using PAX1 methylation analysis (MS-HRM) and high-risk HPV DNA test (HC2), followed by colposcopy and cervical biopsy. Chi-square test were used to test the differences of PAX1 methylation or HPV infection between groups. In the detection of CIN2+, the sensitivity, specificity, the PPV, NPV and the accuracy of PAX1 MS-HRM assay and high-risk HPV (HR-HPV) tests were respectively 80.6% vs 67.7%, 94.9% vs 54.5%, 83.3%, vs 31.8%, 94.0% vs 84.4%, and 91.5% vs 57.7%. The PAX1 MS-HRM assay proved superior to HR-HPV testing in the detection of high grade lesions (CIN2+) in ASC-H. This approach could screen out the majority of high grade lesion cases of ASC-H, and thus could reduce the referral rate to colposcopy.

• The Journal of Korean Society of Virology
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• v.30 no.2
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• pp.131-139
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• 2000

Bovine leukemia virus (BLV) is an etiological agent of chronic diseases in cows worldwide. The BLV is one of retroviruses that contain a multi-functional enzyme, reverse transcriptase produced from the pol gene in its genome. We have sequenced some regions in the pol gene of BLV proviruses found in the Southern province of Korea from samples that turned out to be BL V positives by a PCR analysis. On the 5' side of the BLV pol gene (polymerase region), it was found that there were four leucines located at every 7 amino acids. They can form a leucine zipper motif that was not same as the pol gene of Japanese BLV isolate. The sequencing result of the proviral pol gene in Korean-type BLV also revealed some mutations leading to amino acid changes such as $CCT(Pro){\to}CTC(Leu)$, $AAT(Asn){\to}AAA(Lys)$, and non-sensible variations i.e., $TCT(Ser){\to}TCC(Ser)$, $ATT(Ile){\to}ATC(I1e)$ and $ACG(Thr){\to}ACA(Thr)$. On the 3' side of the pol gene (integrase region), some nucleotide sequences were mutated and led to amino acid changes. Among them, a mutation, $GAA(Glu){\to}GAC(Asp)$ occurred in many Korean-type BLV proviruses was very interesting because the amino acid was regarded as one of the most conserved amino acids in the retroviral integrase. It was also notable that the mutation on any leucine residue did not occur, in spite of its frequent appearance.

• Journal of Korean Society of Transportation
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• v.14 no.2
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• pp.191-222
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• 1996

현재 쓰이고 있는 무신호 교차로의 서비스수준 측정방법은 지체도에 의해서 판정 되어지고 있으며, 안전도를 기준으로 서비스수준을 측정하는 방법은 존재하지 않는다. 교차 로의 안전은 사고의 횟수를 기준으로 하거나, TCT(Traffic Conflict Technique)를 이용하여 측정되어지고 있다. 두 방법 모두 많은 시간과 인력이 요구되는 방법이다. 이에 많은 시간 과 인력투자 없이 교차로의 안전을 진단하는 방법을 개발하는데 이 연구의 목적이 있다. 무 신호 교차로의 안전에 영향을 미치는 요소로는 교차로의 시거, 운전자의 인지반응시간, 차 두간격, 차량속도, 차중, 노면상태, 날씨 등이 있다. 이 모든 요소들이 복합적으로 작용하여 사고를 유발한다. 이 연구에서는 위의 요소들을 모두 고려하여 무신호 교차로의 안전을 분 석하는 방법을 개발하고저 한다. 위의 요소들 중에서 교차로의 시거를 제일 주요한 요소로 보고 나머지 요소들을 첨가시켜서 분석하는 방법을 썼다. 위에 열거한 많은 요소들을 고려 하기 위해서는 Simulation 방법이 채택되었으며, 그 중에서도 Monte carlo Simulationah형 을 썼다. 이 연구에서는 무신호 4지교차로의 횡단차량에 대해서만 고려하였으며 이 연구에 서 개발된 Simulation 모델을 Conflict의 개수와 그때의 소모된 평균 운동에너지를 산출하여 위험도를 측정하는 기준으로 삼았다. 모델의 결과에 의하면 교차로 시거가 길수록 안전하 고, 상대적으로 시거가 짧을수록 위험하다고 나왔다. 또한 AASHTO의 교차로 시거 값은 약 간 하향 조정하여도 안전도에 있어서는 큰 변화가 없는 것으로 분석되었으며, 아울러 안전 에 의한 서비스수준(LOS. A~F)의 기준이 설정되었다. 모델의 결과에 의하여 교통 공학자들 은 어떤 무신호 교차로의 안전수준을 상대적으로 평가할 수 있으며, 교차로 시거의 개선 후 얻어질 편익을 미리 예상할 수 있다.

• KSII Transactions on Internet and Information Systems (TIIS)
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• v.10 no.4
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• pp.1519-1534
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• 2016

In this paper, we study how to optimize the data shuffle phase by leveraging the flow relationship in datacenter networks (DCNs). In most of the clustering computer frameworks, the completion of a transfer (a group of flows that can enable a computation stage to start or complete) is determined by the flow completing last, so that limiting the rate of other flows (not the last one) appropriately can save bandwidth without impacting the performance of any transfer. Furthermore, for the flows enter network late, more bandwidth can be assigned to them to accelerate the completion of the entire transfer. Based on these characteristics, we propose the flow chasing algorithm (FCA) to optimize the completion of the entire transfer. We implement FCA on a real testbed. By evaluation, we find that FCA can not only reduce the completion time of data transfer by 6.24% on average, but also accelerate the completion of data shuffle phase and entire job.

• Journal of fish pathology
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• v.22 no.1
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• pp.85-90
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• 2009

A method for the identification of Nocardia seriolae, the causative agent of nocardiosis in cultured fishes, using PCR was developed in the study. A PCR primer set specific to N. seriolae was designed based on 16S-23S rRNA sequence of various Nocardia species accessed in GenBank. Designed PCR primer set, Nseri-F (5'-GCA AAC TCT TCG AAC AGT CG-3') and Nseri-R (5'-GGA TAT CAG GAC TTA CCG GC-3'), amplifies the target regions of N. seriolae only, but not 4 other Nocardia species, N. asteroides, N. crassostreae, N. farcinica and N. salmonicida.

• Steel and Composite Structures
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• v.28 no.1
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• pp.111-121
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• 2018

It has been argued that fracture energy of composite laminates depends on their thickness and number of layers. In this paper a modified direct energy balance approach (DEBA) has been developed to evaluate the mode-II shear fracture energy for E-glass/Epoxy laminates from finite element model at an arbitrary thickness. This approach considers friction and damage/plasticity deformations using cohesive zone modeling (CZM) and nonlinear finite element modeling. The presence of compressive stress and resulting friction was argued to be a possible cause for the thickness dependency of fracture energy. In the finite element modeling, CZM formulation has been developed with bilinear cohesive constitutive law combined with friction consideration. Also ply element have been developed with shear plastic damage model. Modified direct energy balance approach has been proposed for estimation of mode-II shear fracture energy. Experiments were performed on laminates of glass epoxy specimens for characterization of material parameters and determination of mode-II fracture energies for different thicknesses. Effect of laminate thickness on fracture energy of transverse crack tension (TCT) and end notched flexure (ENF) specimens has been numerically studied and comparison with experimental results has been made. It is shown that the developed numerical approach is capable of estimating increase in fracture energy due to size effect.

• Korean Journal of Remote Sensing
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• v.24 no.2
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• pp.195-203
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• 2008

Burn severity is an important role for rehabilitation of burned forest area. This factor led to the pilot study to determine if high resolution IKONOS images could be used to classify and delinenate the bum severity over burned areas of Samchock Fire and Cheongyang-Yesan Fire. The results of this study can be summarized as follows: 1. The modified Normalized Bum Ratio (NBR) for IKONOS imagery can be evaluated using burn severity mapping. 2. IKONOS-derived NBR imagery could provide fire scar and detail mapping of burned areas at Samchock fire and Cheongyang-Yesan Burns.

• Proceedings of the International Microelectronics And Packaging Society Conference
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• 2004.09a
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• pp.233-241
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• 2004

A new alloy definition will be presented concerning increasing demands for the board level reliability of miniaturized interconnections. The damage mechanism for LFBGA components on different board finishes is not quite understood. Further demands from mobile phones are the drop test, characterizing interface performance of different package constructions in relation to decreased pad constructions and therefore interfaces. The paper discusses the characterization of interfaces based on SnPb, SnPbXYZ, SnAgCu and SnAgCuInNd ball materials and SnAgCuInNd as solder paste, the stability after accelerated tests and the description of modified interfaces strictly related to the assembly conditions, dissolution behavior of finishes on board side and the influence of intermetallic formation. The type of intermetallic as well as the quantity of intermetallics are observed, primaliry the hardness, E modules describing the ability of strain/stress compensation. First results of board level reliability are presented after TCT-40/+150. Improvement steps from the ball formulation will be discussed in conjunction to the implementation of lead free materials In order to optimize ball materials for area array devices accelareted aging conditions like TCTs were used to analyze the board level reliability of different ball materials for BGA, LFBGA, CSP, Flip Chip. The paper outlines lead-free ball analysis in comparison to conventional solder balls for BGA and chip size packages. The important points of interest are the description of processability related to existing ball attach procedures, requirements of interconnection properties and the knowledge gained the board level reliability. Both are the primary acceptance criteria for implementation. Knowledge about melting characteristic, surface tension depend on temperature and organic vehicles, wetting behavior, electrical conductivity, thermal conductivity, specific heat, mechanical strength, creep and relaxation properties, interactions to preferred finishes (minor impurities), intermetallic growth, content of IMC, brittleness depend on solved elements/IMC, fatigue resistance, damage mechanism, affinity against oxygen, reduction potential, decontamination efforts, endo-/exothermic reactions, diffusion properties related to finishes or bare materials, isothermal fatigue, thermo-cyclic fatigue, corrosion properties, lifetime prediction based on board level results, compatibility with rework/repair solders, rework temperatures of modified solders (Impurities, change in the melting point or range), compatibility to components and laminates.