Journal of fish pathology (한국어병학회지)

The Korean Society of Fish Pathology (한국어병학회)
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Identification of Nocardia seriolae by polymerase chain reaction

PCR에 의한 Nocardia seriolae의 검출

  • Park, Myoung-Ae (Pathology Division, National Fisheries Research and Development Institute (NFRDI)) ;
  • Cho, Mi-Young (Pathology Division, National Fisheries Research and Development Institute (NFRDI)) ;
  • Kim, Myoung-Sug (Pathology Division, National Fisheries Research and Development Institute (NFRDI)) ;
  • Kim, Jae-Hoon (Gangreung branch office, National Fisheries Products Inspection Service (NFIS)) ;
  • Lee, Deok-Chan (Pathology Division, National Fisheries Research and Development Institute (NFRDI))
  • 박명애 (국립수산과학원 병리연구과) ;
  • 조미영 (국립수산과학원 병리연구과) ;
  • 김명석 (국립수산과학원 병리연구과) ;
  • 김재훈 (국립수산물품질검사원 강릉지원) ;
  • 이덕찬 (국립수산과학원 병리연구과)
  • Published : 2009.04.01
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Abstract

A method for the identification of Nocardia seriolae, the causative agent of nocardiosis in cultured fishes, using PCR was developed in the study. A PCR primer set specific to N. seriolae was designed based on 16S-23S rRNA sequence of various Nocardia species accessed in GenBank. Designed PCR primer set, Nseri-F (5'-GCA AAC TCT TCG AAC AGT CG-3') and Nseri-R (5'-GGA TAT CAG GAC TTA CCG GC-3'), amplifies the target regions of N. seriolae only, but not 4 other Nocardia species, N. asteroides, N. crassostreae, N. farcinica and N. salmonicida.

본 연구에 사용한 Nseri-F와 Nseri-R primer는 N. seriolae의 16S-23S rRNA 부위에 위치한 영역으로 여러 종의 Nocardia spp.와 비교할 때 PCR 검출 특이성이 매우 높을 것으로 예상되었다. 실제로 이 primer를 이용하여 동물 (N. asteroides 와 N. farcinica), 어류 (N. asteroides, N. salmoniciada 및 N. seriolae) 뿐만 아니라 무척추동물 (N. crassostreae) 등에 감염되는 원인체들에 대하여 실험한 결과 N. seriolae의 특이적 검출이 가능 하였다.

Keywords

References

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