Browse > Article
http://dx.doi.org/10.7314/APJCP.2014.15.2.891

PAX1 Methylation Analysis by MS-HRM is Useful in Triage of High-grade Squamous Intraepithelial Lesions  

Wang, Zhen-Ming (Department of Clinical Laboratory, Weifang City People's Hospital)
Publication Information
Asian Pacific Journal of Cancer Prevention / v.15, no.2, 2014 , pp. 891-894 More about this Journal
Abstract
This study is aimed to investigate the role of paired boxed gene 1 (PAX1) methylation analysis by methylation-sensitive high-resolution melting (MS-HRM) in the detection of high grade lesions in atypical squamous cells cannot exclude high-grade squamous intraepithelial lesion (ASC-H) and compared its performance with the Hybrid Capture 2 (HC2) human papillomavirus (HPV) test. In our study, 130 cases with a diagnosis of ASC-H from the cervical cytological screening by Thinprep cytologic test (TCT) technique were selected for triage. Their cervical scrapings were collected and evaluated by using PAX1 methylation analysis (MS-HRM) and high-risk HPV DNA test (HC2), followed by colposcopy and cervical biopsy. Chi-square test were used to test the differences of PAX1 methylation or HPV infection between groups. In the detection of CIN2+, the sensitivity, specificity, the PPV, NPV and the accuracy of PAX1 MS-HRM assay and high-risk HPV (HR-HPV) tests were respectively 80.6% vs 67.7%, 94.9% vs 54.5%, 83.3%, vs 31.8%, 94.0% vs 84.4%, and 91.5% vs 57.7%. The PAX1 MS-HRM assay proved superior to HR-HPV testing in the detection of high grade lesions (CIN2+) in ASC-H. This approach could screen out the majority of high grade lesion cases of ASC-H, and thus could reduce the referral rate to colposcopy.
Keywords
ASC-H; PAX1 methylation; MS-HRM analysis; HPV testing;
Citations & Related Records
Times Cited By KSCI : 1  (Citation Analysis)
연도 인용수 순위
1 Dimitrakopoulos L, Vorkas PA, Georgoulias V, Lianidou ES (2012). A closed-tube methylation-sensitive high resolution melting assay (MS-HRMA) for the semi-quantitative determination of CST6 promoter methylation in clinical samples. BMC Cancer, 12, 486.   DOI
2 Kristensen LS, Wojdacz TK, Thestrup BB, et al (2009). Quality assessment of DNA derived from up to 30 years old formalin fixed paraffin embedded (FFPE) tissue for PCR-based methylation analysis using SMART-MSP and MS-HRM. BMC Cancer, 9, 453.   DOI   ScienceOn
3 Nambaru L, Meenakumari B, Swaminathan R, Rajkumar T (2009). Prognostic significance of HPV physical status and integration sites in cervical cancer. Asian Pac J Cancer Prev, 10, 355-60.
4 Selvaggi SM (2013). Clinical significance of atypical squamous cells cannot exclude high grade squamous intraepithelial lesion with histologic correlation-: A 9-Year experience. Diagn Cytopathol, 41, 943-6.   DOI   ScienceOn
5 Sherman ME, Castle PE, Solomon D (2006). Cervical cytology of atypical squamous cells-cannot exclude high-grade squamous intraepithelial lesion (ASC-H): characteristics and histologic outcomes. Cancer, 108, 298-305.   DOI   ScienceOn
6 Sherman ME, Solomon D, Schiffman M (2001). Qualification of ASCUS. A comparison of equivocal LSIL and equivocal HSIL cervical cytology in the ASCUS LSIL Triage Study. Am J Clin Pathol, 116, 386-94.   DOI   ScienceOn
7 Bandyopadhyay S, Austin RM, Dabbs D, Zhao C (2008). Adjunctive human papillomavirus DNA testing is a useful option in some clinical settings for disease risk assessment and triage of females with ASC-H Papanicolaou test results. Arch Pathol Lab Med, 132, 1874-81.
8 Candiloro IL, Mikeska T, Dobrovic A (2011). Assessing combined methylation-sensitive high resolution melting and pyrosequencing for the analysis of heterogeneous DNA methylation. Epigenetics, 6, 500-7.   DOI
9 Wang JL, Yang YZ, Dong WW, et al (2013). Application of human papillomavirus in screening for cervical cancer and precancerous lesions. Asian Pac J Cancer Prev, 14, 2979-82.   과학기술학회마을   DOI   ScienceOn
10 The ASCUS-LSIL Triage Study (ALTS) Group (2003). Results of a randomized trial on the management of cytology interpretations of atypical squamous cells of undetermined significance. Am J Obstet Gynecol, 188, 1383-92.   DOI
11 Wright TC, Jr., Cox JT, Massad LS, Twiggs LB, Wilkinson EJ (2002). 2001 Consensus Guidelines for the management of women with cervical cytological abnormalities. JAMA, 287, 2120-9.   DOI   ScienceOn
12 Walboomers JM, Jacobs MV, Manos MM, et al (1999). Human papillomavirus is a necessary cause of invasive cervical cancer worldwide. J Pathol, 189, 12-9.   DOI
13 Wittwer CT, Reed GH, Gundry CN, Vandersteen JG, Pryor RJ (2003). High-resolution genotyping by amplicon melting analysis using LCGreen. Clin Chem, 49, 853-60.   DOI   ScienceOn
14 Wojdacz TK, Dobrovic A (2007). Methylation-sensitive high resolution melting (MS-HRM): a new approach for sensitive and high-throughput assessment of methylation. Nucleic Acids Res, 35, e41.   DOI   ScienceOn
15 Wu HH, Allen SL, Kirkpatrick JL, Elsheikh TM (2006). Reflex high-risk human papilloma virus DNA test is useful in the triage of women with atypical squamous cells cannot exclude high-grade squamous intraepithelial lesion. Diagn Cytopathol, 34, 707-10.   DOI   ScienceOn
16 Wu W, Zhang J, Yang H, Shao Y, Yu B (2011). Examination of AKAP12 promoter methylation in skin cancer using methylation-sensitive high-resolution melting analysis. Clin Exp Dermatol, 36, 381-5.   DOI   ScienceOn
17 Lai HC, Lin YW, Huang TH, et al (2008). Identification of novel DNA methylation markers in cervical cancer. Int J Cancer, 123, 161-7.   DOI   ScienceOn
18 Carreon JD, Sherman ME, Guillen D, et al (2007). CIN2 is a much less reproducible and less valid diagnosis than CIN3: results from a histological review of population-based cervical samples. Int J Gynecol Pathol, 26, 441-6.   DOI   ScienceOn