• KOREAN JOURNAL OF CROP SCIENCE
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• v.54 no.1
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• pp.1-6
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• 2009

This study is to analyze the effects of the growth temperature of mungbean sprouts ($15{\sim}30{\pm}1^{\circ}C$) on the yield ratio, content of phenolic compounds and DPPH (1,1-diphenyl-2-picrylhydrazyl), ADH (alcohol dehydrogenase), ALDH (aldehyde dehydrogenase) activities of the sprouts. When the growth temperature of mungbean sprouts was higher, the yield ratio of the sprouts was higher while the hard seed rate was lower, but $25{\pm}1^{\circ}C$ and $30{\pm}1^{\circ}C$ showed no regular tendency. The content of the total phenol from the ethanol extract of the sprouts was higher in the growth temperature of $15{\pm}1$, $20{\pm}1$, and $25{\pm}1^{\circ}C$, while the content of total flavonoid was higher in the growth temperature of $15{\pm}1$, and $20{\pm}1^{\circ}C$. The DPPH radical scavenging activity of the ethanol extract of the sprouts was higher when the growth temperature was lower, while the activity of ADH and ALDH showed no regular tendency according to the growth temperature. Considering the yield ratio, content of phenolic compounds, biological activities of mungbean sprouts, the optimum cultivation temperature of mungbean sprouts may be $20{\sim}25^{\circ}C$.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.53 no.3
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• pp.273-278
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• 2008

This study set out to investigate the changes to the growth, yield, and flavonoid contents of mungbean according to different seeding times from mid May to mid July in the southern region of Korea. Days to first flowering, days to first maturing, and cultivation period were shorter at later seeding time than earlier seeding time. But later the seeding time was, days from first maturing to first harvesting, days required between harvesting increased. Number of pods at first harvesting and yield of mungbean were highest when seeded at late June, showing increase in yield 14% more than at early June as standard seeding time. However number of seeds per pod and 1,000-seed weight at first harvesting were highest when seeded at mid July. The number of harvesting was smallest at two times when seeded at June or mid July. The contents of average vitexin and isovitexin in mungbean were highest in the order of mid July, late June, and mid May. In particular, their contents reached its highest point in the seeds of the second harvest. Considering the results of the cultivation period, yield, harvesting times, vitexin and isovitexin contents, the proper seeding time of mungbeans in the southern region of Korea will be from late June to mid July.

• Journal of Digital Convergence
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• v.12 no.11
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• pp.461-468
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• 2014

This study was to figure out the influence of oral health behavior and periodontal status on their OHIP-14. Explained to 255 people who visited the scaling center of Dental Hygienics of a college in Gyeongbuk from March 7th to June 13th 2014 and it was agreed to perform. Result of dental plaque and calculus rate of those with no TBI were high; the less frequent do they teeth brushing the higher were their rate of dental plaque, bleeding and calculus, while their OHIP-14 was low. The rate of calculus of whom with no education on dental floss use and who do not use dental flosswas high. The more powerful for those with higher rate of dental plaque; while the lower was the rate of calculus the higher was OHIP-14. Therefore, the more do they brush their teeth and the lower are the rate of their calculus, the more positively has it been figured out to influence on the quality of their life of oral health. Consequently, public health program should be developed to educate them on the necessity of scaing on a regular basis and the cause calculus of occurrence, as well as a media through which they can easily participate in the program.

• Journal of dental hygiene science
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• v.17 no.2
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• pp.183-191
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• 2017

The purpose of this study was to evaluate a clinical preventative care program, based on a dental hygiene process (accessment, diagnosis, planning, implementation, evaluation; ADPIE) in a dental clinic, by analyzing patient recognition, knowledge, practice, and satisfaction with respect to oral health. The collected data (in percentages) were analyzed Fisher's exact test and paired t-test using IBM SPSS ver. 21.0 (IBM Co., USA). This study demonstrated a significant difference in oral health knowledge, recognition, and practice before and after the clinical preventive care program (p<0.05). The results were significant in the individual preventive plan within the planning stage, and in the professional teeth cleaning implementation stage (p<0.05). This result can be attributed to the sympathy of the dental hygienist (p<0.05). There was a positive correlation between recommending dental checkups and regular checking of the (r=0.552, p<0.05), undergoing radiography (r=0.434, p<0.01), following an individual preventive plan (r=0.568, p<0.01), undergoing proximal machine teeth cleaning (r=0.437, p<0.05), following tooth brushing instructions (r=0.552, p<0.05), and the evaluation results (r=1.000, p<0.05). Our results demonstrate, that the clinical preventive care program, based on dental hygiene, is an effective program. Given the positive effect of dental revisits and patient recommendations promoting dental hygienists, it is hoped that this preventative program will be widely used.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.53 no.4
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• pp.353-358
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• 2008

The purpose of this study was to examine the effects of black polyethylene film mulching and soil conditioners on the growth, yield, and flavonoids biosynthesis of mungbeans. The seedling stand rate, plant height, and pods per plant were 98%, 61 cm, and 15.1, respectively, when the mungbean grown black polyethylene film mulches. They were greater than these results when the without mulches was applied by 13%, 9 cm, and 1.8, respectively. Mungbean grown with black polyethylene film mulches ($243\;kg{\cdot}10a^{-1}$) exhibited 38% more seed yield than without mulches ($176\;kg{\cdot}10a^{-1}$). However, no significant difference in contents of vitexin and isovitexin of mungbeans between with black polyethylene film mulches and without mulches was observed. Among soil conditioners, standard fertilizer+oil cake and standard fertilizer+mineral powders increased number of pods. The standard fertilizer+oil cake showed the highest seed yield (119%) of mungbean when it mixed with standard fertilizers, and followed by mineral powders (115%). The mixed combinations of oil cake or mineral powders also seem to have affected the biosynthesis of vitexin and isovitexin, whose content in mungbeans was significantly more.

• Korean Journal of Plant Resources
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• v.22 no.2
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• pp.128-135
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• 2009

In this study, the selected 789 lines having agronomic values out of over 2,500 mungbean (Vigna radiata (L.) Wilczek) germplasms, examined the 27 characters including hypocotyl color and leaf size of 401 lines in 2005 and of 388 lines in 2006, and analyzed the contents of vitexin and isovitexin in these mungbean germplasms. The average contents of vitexin and isovitexin in mungbean of the 401 lines used in 2005 were 8.71($1.1{\sim}13.4$) and 9.54($0.9{\sim}15.9$) mg/g, respectively, and the correlation coefficiency ($R^2$) of the contents of vitexin and isovitexin was 0.958. Nine lines including VC3890B were selected to be the ones containing high vitexin and isovitexin. The average contents of vitexin and isovitexin in mungbean of the 388 lines used in 2006 were 10.17($2.0{\sim}15.9$) and 10.64($0.2{\sim}17.6$) mg/g, respectively, and the correlation coefficiency ($R^2$) of the contents of vitexin and isovitexin was 0.958. Six lines including VC4096-2B-4-B-2-B were selected to be the ones containing high vitexin and isovitexin. The contents of vitexin and isovitexin were higher in the mungbean with larger leaves and longer ripening period out of the main characters.

• Animal Bioscience
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• v.34 no.5
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• pp.867-879
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• 2021

Objective: Fibronectin 3 (FN3) and immunoglobulin like modules (Ig) are usually collocated beside modular cellulase catalytic domains. However, very few researches have investigated the role of these modules. In a previous study, we have sequenced and analyzed bacterial metagenomic DNA in Vietnamese goats' rumen and found that cellulase-producing bacteria and cellulase families were dominant. In this study, the properties of modular cellulases and the role of a FN3 in unique endoglucanase belonging to glycosyl hydorlase (GH) family 5 were determined. Methods: Based on Pfam analysis, the cellulases sequences containing FN3, Ig modules were extracted from 297 complete open reading frames (ORFs). The alkaline, thermostability, tertiary structure of deduced enzymes were predicted by AcalPred, TBI software, Phyre2 and Swiss models. Then, whole and truncated forms of a selected gene were expressed in Escherichia coli and purified by His-tag affinity column for assessment of FN3 ability to enhance enzyme activity, solubility and conformation. Results: From 297 complete ORFs coding for cellulases, 148 sequences containing FN3, Ig were identified. Mostly FN3 appeared in 90.9% beta-glucosidases belonging to glycosyl hydrolase family 3 (GH3) and situated downstream of catalytic domains. The Ig was found upstream of 100% endoglucanase GH9. Rarely FN3 was seen to be situated downstream of X domain and upstream of catalytic domain endoglucanase GH5. Whole enzyme (called XFN3GH5 based on modular structure) and truncate forms FN3, XFN3, FN3GH5, GH5 were cloned in pET22b (+) and pET22SUMO to be expressed in single and fusion forms with a small ubiquitin-related modifier partner (S). The FN3, SFN3 increased GH5 solubility in FN3GH5, SFN3GH5. The SFN3 partly served for GH5 conformation in SFN3GH5, increased modules interaction and enzyme-soluble substrate affinity to enhance SXFN3GH5, SFN3GH5 activities in mixtures. Both SFN3 and SXFN3 did not anchor enzyme on filter paper but exfoliate and separate cellulose chains on filter paper for enzyme hydrolysis. Conclusion: Based on these findings, the presence of FN3 module in certain cellulases was confirmed and it assisted for enzyme conformation and activity in both soluble and insoluble substrate.

• The korean journal of orthodontics
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• v.29 no.3 s.74
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• pp.361-374
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• 1999

Intraoral filled type of orthodontic appliance can cause reversible or irreversible damages such as gingivitis, periodontitis, enamel decalcification, dental caries, root resorption, and pulpal changes. Such adverse effects are brought by increase in dental plaque as well as oral flora. Such an increase causes gingival inflammation and enamel decalcification. The purpose of this study is to get klowledge on initial changes in dental plaque, gingivitis, and enamel decalcification after bonding fixed orthodontic appliances according to time flow, gender, and sides(right/left) of premolar region. For control group, 48 students of dental college, Yonsei university(26 males, 22 females) were chosen; for experimental group, 73 orthodontic patients(36 males, 37 females) who will be treated with fixed appliances were chosen. All the subjects had no systemic disease, juvenile periodontitis and all the females had passed their ,menarche. Tooth brushing instruction was given to all the subjects prior to the experiment. For control group, plaque index, gingival index, and decalcification index were measured twice at 3 weeks interval ; for experimental group, the same was done prior to, 3, 6, 9 weeks after bonding fixed appliances. The following results were obtained: 1. In plaque index 3 weeks after placement of appliances, and it showed gradual increase afterwards. 2. In gingival index3 weeks after placement of appliances, and afterwards it showed increase at a faster rate than plaque index. 3. Enamel decalcification began to show between 3 and 6 weeks after bonding fixed appliances. Decalcification index began to increase 6 weeks after appliance placement, but there was no statistical significance. 4. When the comparison was made between two sides of premolar region, the right side showed greater index in plaque and gingival index of experimental group.

• Journal of the Korean Society of Food Science and Nutrition
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• v.36 no.11
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• pp.1409-1416
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• 2007

Seven selected commercial pure or refined olive oils were obtained from the market, and their physicochemical properties and volatile characterizations were investigated. Fatty acid profiles of the analyzed olive oils showed oleic $(61.2{\sim}74.7mole%)$, palmitic $(10.2{\sim}16.8mole%)$, linoleic $(9.4{\sim}18.0mole%)$, stearic $(1.9{\sim}3.0mole%)$, palmitoleic $(0.7{\sim}2.4mole%)$ and linolenic acid $(0.5{\sim}0.9mole%)$. According to Hunter#s color measurement, pure or refined olive oils showed $L^*$ value of $92.2{\sim}99.0$, $a^*$ value of $-22.2{\sim}-3.2$, and $b^*$ value of $18.5{\sim}55.0$. Their total phenol contents ranged from 1.9 to $13.3mg/100g$ while ${\alpha}-tocopherol$ content showed $7.91{\sim}13.88mg/100g$. Oxidation stability of the pure or refined olive oils were observed by Rancimat. The induction period ranged from 17.37 to 34.72 hr while their POV were $6.83{\sim}20.31meq/kg$ oil. Electronic nose and gas chromatograph-mass spectrometry with head-space solid phase microextraction were applied to identify and discriminate the volatile compounds and flavors in pure or refined olive oils, respectively.

• KSBB Journal
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• v.24 no.6
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• pp.541-548
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• 2009

For investigation of anti-angiogenesis mechanism of Anthrisci radix, Psoraleae semen, Siegesbeckiae herba and Corni fructus, anti-cell adhesion experiment was performed. The adhesion of U937 cells to IL-$1\beta$-stimulated HUVECs was completely suppressed by 276% at 0.2 mg/L of Anthrisci radix, 220% at 5 mg/L of Psoraleae semen, 158% at 10 mg/L of Siegesbeckiae herba and 132% at 20 mg/L of Corni fructus, respectively. And the adhesion of PMA-chemical stimulated U937 cells to HUVECs, it was inhibited 139% at 0.2 mg/L of Anthrisci radix, 442% at 5 mg/L of Psoraleae semen, 720% at 10 mg/L of Siegesbeckiae herba and 664% at 20 mg/L of Corni fructus. Also, the adhesion of chemical stimulated U937 cells to IL-$1\beta$/chemical stimullated HUVECs, it was inhibited by 286% at 0.2 mg/L of Anthrisci radix, 146% at 5 mg/L of Psoraleae semen, 436% at 10 mg/L of Siegesbeckiae herba and 297% at 20 mg/L of Corni fructus, respectively. It would be a useful substance for anti-cell adhesion based on anti-angiogenesis for anti-obesity and anti-cancer.