• Asian-Australasian Journal of Animal Sciences
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• v.29 no.4
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• pp.500-508
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• 2016

This study was conducted to investigate the effects of Momordica charantia saponin (MCS) on ruminal fermentation of maize stover and abundance of selected microbial populations in vitro. Five levels of MCS supplements (0, 0.01, 0.06, 0.30, 0.60 mg/mL) were tested. The pH, $NH_3-N$, and volatile fatty acid were measured at 6, 24, 48 h of in vitro mixed incubation fluids, whilst the selected microbial populations were determined at 6 and 24 h. The high dose of MCS increased the initial fractional rate of degradation at t-value = 0 ($FRD_0$) and the fractional rate of gas production (k), but decreased the theoretical maximum of gas production ($V_F$) and the half-life ($t_{0.5}$) compared with the control. The $NH_3-N$ concentration reached the lowest concentration with 0.01 mg MCS/mL at 6 h. The MSC inclusion increased (p<0.001) the molar proportion of butyrate, isovalerate at 24 h and 48 h, and the molar proportion of acetate at 24 h, but then decreased (p<0.05) them at 48 h. The molar proportion of valerate was increased (p<0.05) at 24 h. The acetate to propionate ratio (A/P; linear, p<0.01) was increased at 24 h, but reached the least value at the level of 0.30 mg/mL MCS. The MCS inclusion decreased (p<0.05) the molar proportion of propionate at 24 h and then increased it at 48 h. The concentration of total volatile fatty acid was decreased (p<0.001) at 24 h, but reached the greatest concentration at the level of 0.01 mg/mL and the least concentration at the level of 0.60 mg/mL. The relative abundance of Ruminococcus albus was increased at 6 h and 24 h, and the relative abundance of Fibrobacter succinogenes was the lowest (p<0.05) at 0.60 mg/mL at 6 h and 24 h. The relative abundance of Butyrivibrio fibrisolvens and fungus reached the greatest value (p<0.05) at low doses of MCS inclusion and the least value (p<0.05) at 0.60 mg/mL at 24 h. The present results demonstrates that a high level of MCS quickly inhibits in vitro fermentation of maize stover, while MCS at low doses has the ability to modulate the ruminal fermentation pattern by regulating the number of functional rumen microbes including cellulolytic bacteria and fungi populations, and may have potential as a feed additive applied in the diets of ruminants.

• Asian-Australasian Journal of Animal Sciences
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• v.30 no.11
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• pp.1620-1632
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• 2017

Objective: This study evaluated the effects of a traditional Chinese medicine formula (TCMF) on muscle fiber characteristics in finishing pigs and the effects of the formula's extract (distilled water, ethyl acetate and petroleum ether extraction) on porcine cell proliferation and isoforms of myosin heavy chain (MyHC) gene expression in myocytes. Methods: In a completely randomized design, ninety pigs were assigned to three diets with five replications per treatment and six pigs per pen. The diets included the basal diet (control group), TCMF1 (basal diet+2.5 g/kg TCMF) and TCMF2 (basal diet+5 g/kg TCMF). The psoas major muscle was obtained from pigs at the end of the experiment. Muscle fiber characteristics in the psoas major muscle were analyzed using myosin ATPase staining. Cell proliferation was measured using 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) dye and cytometry. Isoforms of MyHC gene expression were detected by real-time quantitative polymerase chain reaction. Results: The final body weight and carcass weight of finishing pigs were increased by TCMF1 (p<0.05), while the psoas major muscle cross-sectional area was increased by TCMF (p<0.05). The cross-sectional area and diameter of psoas major muscle fiber Ι, IIA, and IIB were increased by TCMF2 (p<0.05). The cross-sectional area and fiber diameter of psoas major muscle fiber IIA and IIB were increased by diet supplementation with TCMF1 (p<0.05). Psoas major muscle fiber IIA and IIB fiber density from the pigs fed the TCMF1 diet and the type IIB fiber density from the pigs fed the TCMF2 diet were lower compared to pigs fed the control diet (p<0.05). Pigs fed TCMF2 had a higher composition of type Ι fiber and a lower percentage of type IIB fiber in the psoas major muscle (p<0.05). The expression levels of MyHC Ι, MyHC IIa, and MyHC IIx mRNA increased and the amount of MyHC IIb mRNA decreased in the psoas major muscle from TCMF2, whereas MyHC Ι and MyHC IIx mRNA increased in the psoas major muscle from TCMF1 (p<0.05). Peroxisome proliferator-activated receptor ${\gamma}$ $coactivator-1{\alpha}$ and CaN mRNA expression in the psoas major muscle were up-regulated by TCMF (p<0.05). Porcine skeletal muscle satellite cell proliferation was promoted by $4{\mu}g/mL$ and $20{\mu}g/mL$ TCMF water extraction (p<0.05). Both $1{\mu}g/mL$ and $5{\mu}g/mL$ of TCMF water extraction increased MyHC IIa, MyHC IIb, and MyHC IIx mRNA expression in porcine myocytes (p<0.05), while MyHC Ι mRNA expression in porcine myocytes was decreased by $5{\mu}g/mL$ TCMF water extraction (p<0.05). Porcine myocyte MyHC Ι and MyHC IIx mRNA expression were increased, and MyHC IIa and MyHC IIb mRNA expression were down-regulated by $5{\mu}g/mL$ TCMF ethyl acetate extraction (p<0.05). MyHC Ι and MyHC IIa mRNA expression in porcine myocytes were increased, and the MyHC IIb mRNA expression was decreased by $1{\mu}g/mL$ TCMF ethyl acetate extraction (p<0.05). Four isoforms of MyHC mRNA expression in porcine myocytes were reduced by $5{\mu}g/mL$ TCMF petroleum ether extraction (p<0.05). MyHC IIa mRNA expression in porcine myocytes increased and MyHC IIb mRNA expression decreased by $1{\mu}g/mL$ in a TCMF petroleum ether extraction (p<0.05). Conclusion: These results indicated that TCMF amplified the psoas major muscle cross-sectional area through changing muscle fiber characteristics in finishing pigs. This effect was confirmed as TCMF extraction promoted porcine cell proliferation and affected isoforms of MyHC gene expression in myocytes.

• Journal of Korean Medical classics
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• v.4
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• pp.19-73
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• 1990

Today, many people are more interested in preventing the disease than curing it. Chi-Kung (氣功) is the way of Life-Cultivation (養生法) peculiar to the orient, it is reported in china that Chi-Kung has an excellent curative value not only in curing the disease but also in preventing it. But the full-scale study of Chi-Kung is not be made up to now in Korea, so I studied the developmental history of chinese Chi-Kung through the oriental medical books. From this study, I reached the following conclusions ; 1. Chi-Kung is naturally derived from the self-preservation instinct to adapt oneself to circumstances of the nature, but in the investigation from the documentational records, it is originated in the treatment method of the Sam-Huang-O-Jae (三皇五帝 )period to cure the abnormal circulation of the vital force and blood caused by damp (濕). 2. As the principle and the method of the Life-Cultivation of the Chun-chu-Jeon-Kook (春秋戰國) periods were recorded in Huang-Jae-Nai-Gyung (黃帝內徑) detailly and the remedy examples by ancient Chi-Kung such as Tao-Yin (導引), Haeng-Chi (行氣) were presented, we considered that theoretical basis of the development of Life-cultivation and Chi-Kung study was furnished in that period. 3. A famous doctor, Hwa-Ta (華陀) lived in Han dynasty, researched the theory and practice of Tao-Yin transmitted from the former generations, as that result, he formed a kind of medical gymnastics what is called O-Keum-Hi (五禽戱). It is considered that 'O-Keum-Hi' is a Tao-Yin method developed more practically and systemetically than the Tao-Yin appeared in the 'Jang-Ja' (莊子) or 'Hoy-Nam-Ja' (淮南子). 4. In Wui-Jin-Nambook Jo (魏晋南北朝) periods, the contents of Chi-Kung were more abundant under the influence of Buddhism (佛敎) and Taoism (道敎). Galhong (葛洪), the author of 'Po-Bak-Ja' (抱朴子) arranged the ancient Chi-Kung method systematically first of all, Tao-Goeng-Gyung (陶宏景), the author of 'Yang-Seong-Yeun-Myung-Rok' (養性延命錄) recorded the 'Yook-Ja-Geul' (六字訣) first time. 5. There is a new development of Chi-Kung therapy in Soo-Tang-Odae (隋唐五代) pefiods, especially So-Won-Bang (巢元方), the author of 'Jey-Byung-Won-Hwu-Ron' (諸病源候論) collected aimost all of the Chi-Kung method, for curing the disease formed before soo (隋) period. From that fact, we supposed that Chi-Kung was utilized more widely in curing the disease. 6. 'So-Ju-Cheon-Hwa-Hu-Peob' (小周天火候法) was adopted as the best orthodox approach under the influence of Nae-Tan-Taoist (道敎內丹學派) in Song-Keum-Won (宋金元) periods, especially in the song dynasty, 'Pal-Dan-Geum' (八段錦) was appeared and assignment of six-Chi (六氣) for bowel and viscera in the 'Yook-Ja-Geul' (六字訣) was decided firmly, that is to say Lung-Si (肺-呬), Heart-Kha (心-呵), Spleen-Hoa (脾-呼), Liver-Hoe (肝-噓), Kidneychui (賢-吹), Three-Burner-shi (三焦-嘻). 7. In Myung-Cheong (明淸) periods, The general practitioner applied the principle of 'Byun-Jeng-Ron-Chi' (辨證論治) to the Chi-Kung field, and after Myeong dynasty the style of doing 'Yook-Ja-Gyel' (六字訣) was developed to the moving style. 8. Today, in china, the study on the Chi-Kung is being progressed constantly under the positive assistance of government, Chi-Kung-Hak (氣功學) has taking its place as a branch of study step by step. It is considered that the establishment of Chi-Kung-Hak Classroom (氣功學教室) and Medical Chi-Kung Center (氣功療法室) for special and systematic research are needed, at the same time the settlement of institutional system for training the Chi-Kung technician (氣功師) is also needed.

• Journal of Sericultural and Entomological Science
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• no.12
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• pp.37-45
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• 1970

The processing management forms of our country's filature factories in 1969 are summarized as follows. (1) About 80％ of total cocoon collection is made within 5 days involving peak day, and 10％ of cocoon collection is finished until 3 days before and after the peak day, (2) About 92％ of alive cocoons transported on unpaved road, and about 40％ of the cocoons purchased by all factories are loaded on trucks from common selling station which is far beyond 40km, therefore a new packing system of alive cocoons to drop the damage of cocoon qualities, should be taken. (3) 22％ of all factories in our. country have only low-temperature cocoon drying machine. Therefore the installment of hot-air cocoon drying machine is required urgently. (4) In view of cocoon qualities in our country, the grouping method of cocoon for reeling. taken by about 50％ of the factories at percent, which classify cocoons for reeling as high group (1,2,3,4 grades) and low group(5,6 grades), will have to be replaced by the method tat classify them high group (1,2 grades) middle group (3,4 grades), low group (5,6 grades). (5) The .ratio of cocoon assorting stood about 10％ in multi-ends reeling, about 15％ in automatic reeling, conclusively, the ratio of cocoon assorting for automatic reeling was higher tan that for multi-ends reeling. One person's ability for a day in cocoon assorting reaches to about 80-100kg. (6) Cocoon cooking condition requires the increase of the cooking time, the pressure and temperature used to be prolonged as much as the qualities of cocoons are material cocoon ior automatic and double cocoon machines are treated uncompletely. (7) Automatic silk reeling is being performed at 1-2$^{\circ}C$ lower in reeling water temperature and operated at about twice velocity. (8) The temperature and humidity of rereeling room stood at 25$^{\circ}C$, 67.2％ R.H and 32.3$^{\circ}C$, 51.9％ R.H of rereeling machine are showed, Average rereeling velocity is 233m/min and large reefs charged for one person are 7.5 reels and form of skein used in all factories is double skein. (9) About 73％ of water sources for filature used under-earth water. About 48％ of all filature factories in our country have not yet water purifying equipments. Installation of the equipment for these factories seems to be urgent, (10) Denier .balance, sizing reel, seriplane, are being used in most factories as self-inspection apparatus. (11) More than 90％ of the factories use the vacum tank in rereeling process and about 20％ of them use it in cocoon cooing process (12) Only 21％ of the factories use chemicals in filature process. About all them use "Seracol 100" in cocoon cooking process and "Seracol 500" in rereeling process, (13) Above survey results explain each all factories show large difference in the processing management. Therefore, it is believed that intercommunication through seminar or technical exchange will contribute to the production evaluation of cocoon in our filature industry.

• Korean journal of applied entomology
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• v.3
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• pp.31-39
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• 1964

The authors studied on the bionomics of the Florida wax scale, Ceroplastes floridensis Comstock and bionomics of two kind of parasites which attack on this Florida wax scale in 1963 to 1964. 1. Ceroplastes floridensis Comstock was heavily infested on the persimmon tree planted in the southern part of Korea, specially at the center of Taegu city. Parasitic ratio of important parasite of Ceroplastes floridensis Comstock, Microterys clauseni was $66.67\%$ and appeared low parasited ratio at the center of the city. 2. The developed eggs of the Ceroplastes floridensis Comstock was appear며 from mid-May to early July. The peak of it was late-May to early June. Average stored eggs inside of a body is 2030. $2030.77\pm0.49.$. 3. The hatching period of the Ceroplastes floridensis Comstock begins from early June to early July. Mid-June was the peak period of it. Pupation period of the male was August 14 to September 7 and adult was emerged on August 20 to September 8. Mean longevity for adult male was 3 days in August but 4 days in September. 4. On the differences of the body- length parasited on the three host plants, persimmon, platanus, and trifoliate orange, the body length parasited on the persimmon was the largest, medium on platanus and the smallest on the trifoliate orange. 5. Microterys clauseni H. Compere parasite on tile Ceroplastes floridensis Comstock with 5 generations in a year. Hibernate in the body of the host as tan·at stage. The first adult appears in middle April. Fifteen days was required for one generation in $21^{\circ}$. The authors observed on the form ovary, female with 6 ovarioles, and mean number of eggs per female was $47.19\pm0.55$. 6. On the parasited individuals of the Microterys clauseni H. Compere a larva was the most numerous and was found 2 individuals or 4 individuals parasite on the one Ceroplastes floridensis Comstock. 7. Coccophagus app. parasited on the only male body of the Ceroplastes floridensis Comstock. The parasitic ratio was $14.831\%$ on persimmon tree in Taegu city.

• The Journal of the Petrological Society of Korea
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• v.7 no.1
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• pp.15-26
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• 1998

The Dabie-Sulu ultra-high pressure metamorphic (UHPM) zone is commonly suggested to be a collisional belt between the Sino-Korea craton (North China craton) and Yangtze craton (Zhai and Cong, 1996). Two important questions in formulating the tectonic evolution of the northeast Asia are : (1) the boundary between the UHPM zone and the Sino-Korea craton in the Shandong peninsula and (2) the extension of this Chinese UHPM zone into the Korean peninsula. There have been different opinions on the boundary between UHPM zone and the Sino-Korea craton in the Shandong peninsula. For example, the boundary has been suggested to be the Tan-Lu fault (Bai et al., 1993; Wang and Cong, 1996), or Wulian-Rongcheng fault (Cao et al., 1990). Our recent study finds out new evidences, indicating that the possible boundary is the Kunyushan granitoid complex zone, which occurs along the Wulian-Muping fault. Our new evidences are : (1) the basic rocks west to the Kunyushan granitoid zone are high-pressure granulites rather than eclogites (Zhai, 1996) with their Sm-Nd isotopic ages of 1750 Ma and 2788 Ma, representing their retrograde metamorphic and petrogenetic ages, respectively (Li et al., 1997b); (2) the orthogneisses west to the Kunyushan granitoid zone yield 2600-2900 Ma zircon ages and 1600-2020 Ma Rb-Sr and chemical U-Th-total Pb ages, with no younger data (Enami et al., 1993; Ishizaka et al., 1994), having a typical characteristic for the early Precambrian rocks in the Sino-Korea craton; (3) the orthogneisses east to the Kunyushan granitoid zone have 110-320 Ma isotopic ages with a peak value of 180-230 Ma, showing a typical characteristic of metamorphic rocks in the UHPM zone; (4) the Kunyushan granitoid zone consists of numerous granitic bodies, stocks and veins, which have 1900-2000 Ma, 610-710 Ma and 124-180 Ma istotopic ages indicating a long and complicated evolution history of this granitoid zone. There are many lenses and enclosures of metamorphic rocks from the Sino-Korea craton and Sulu UHPM belt in the Kunyushan granitoid zone. Zhai et al. (1998) have defined the Kunyushan granitoid zone as the Jiaodong Boundary complex zone. Some geologists suggested that the UHPM zone extend eastward to the Korea peninsula (Yin and Nie, 1993; Wang and Cong, 1996) and possibly to the Imjingang belt (Chang, 1994; Ree et al., 1996). Unfortunately, there has not been a conclusive evidence indicating that UHPM rocks occur in the Korea peninsula. In this regard, it becomes more important to compare metamorphic rocks in the Shandong peninsula with those in northern and southern Korea peninsula.

• Proceedings of the Korean Institute of Information and Commucation Sciences Conference
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• v.9 no.1
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• pp.397-402
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• 2005

In the Korean seas, Sea Surface Temperature (SST) and Thermal Fronts (TF) were analyzed temporally and spatially during 8 years from 1993 to 2000 using NOAA/AVHRR MCSST As the result of EOF method applying SST, the variance of the 1st mode was 97.6%. It is suitable to explain SST conditions in the whole Korean seas. Time coefficients were shown annual variations and spatial distributions were shown the closer to the continent the higher SST variations like as annual amplitudes. The 2nd mode presented higher time coefficients of 1993, 94, and 95 than those of other years. Although the influence is a little, that tan explain EININO effort to the Korean seas. TF were detected by Sobel Edge Detection Method using gradient of SST. Consequently, TF were divided into 4 fronts; the Subpolar Front (SPF) dividing into the north and south part of the East sea , the Kuroshio Front (KF) in the East China Sea (ESC), the South Sea Coastal Front (SSCF) in the South sea, and the Tidal Front in the West sea. TF located in steep slope of submarine topography. The distributions of 1st mode in SST were bounded in the same place, and these results should be considered to influence of seasonal variations. To discover temporal and spatial variations of TF, SST gradient values were analyzed by EOF. The time coefficients fo the 1st mode (variance : 64.55%) showed distinctive annual variations and SPF, KF, and SSCF was significantly appeared in March. the spatial distributions of the 2nd mode showed contrast distribution, as SPF and SSCF had strong'-'value, where KF had strong'+'value. The time of'+'and'-'value was May and October, respectively. Time coefficients of the 3rd mode had 2 peaks per year and showed definite seasonal variations. SPF represented striking'+'value which time was March and October. That was result reflected time of the 1st and 2nd mode. We can suggest specific temporal and spatial variations of TF using EOF.

• Journal of the Korean Institute of Traditional Landscape Architecture
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• v.36 no.3
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• pp.37-47
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• 2018

This study was attempted to understand present status and characteristics of landscape components of the domestic Korean Gugokwonlim created by the classical scholars in the Joseon Dynasty. The results are as follows. First, Distribution of Gukokwonlim in Korea shows that 55(51.4%) are located in Daegu and Gyeongsangbuk-do, and 22(20.56%) are located in Chungcheongbuk-do. Concentrated locations of Gugok are on the part of Baekdudaegan, from Sobaeksan mountain to Sokrisan mountain via Wolaksan mountain, and the Nakdong River basin of the Nakdong vein in the right bank. This consideration seems to be closely related to the academy of Yeongnam Confucianism and the their trend of Wonrim enjoyment. Second, according to the result of examining the distribution of Gugok according to the basic local government authority, The biggest number of the Gugok places(10 places, 9.35%) are located in Andong, which is called 'the capital of Korean spiritual culture.' Additionally in order, 9 places(9.45%) is located in Goesan, 8 places(7.48%) in Mungyung, 6 places(5.61%) in Bonghwa, and 5 places(4.67%) in Yeongju. Third, in order to the creating time of Gugokwonlim, 33 (33.0%) were created in $18^{th}$ century, and other 33 (33.0%) were created in $19^{th}$ century. In addition, 14 were created during $20^{th}$ century, while 13 were created in $17^{th}$ century. And 4 were created in $16^{th}$ century. Respectively. great number of $18^{th}$ and $19^{th}$ centuries shows that many(66.0%) Gugokwonrim were created between late 18th to 19th centuries. Fourth, There were 97(90.65%) of 'Gugok' in the form of collecting type, and a total number of bottom-up style Gugok were 99(92.5%) while top-down style Gugok were 8(7.5%). Fifth, Among the contents of Gugok, 67 were found in pome of Gugok(64.49%), 29 caved letters in rock(27.10%), and 16 in painting of Gugok(14.95%). Sixth, The most emerged landscape components of Gugok was Dae(臺) 124(13.05%), followed by Am(巖) 115[11.2%, including of Am(岩)] 115(11.2%), and Dam(潭) 73(7.68%), Jeong(亭) 48(5.05%), Dong(洞) 39(4.10%), San(山) 36(3.78%), Am(岩, rocks) 31(3.26 %), Bong(峯, peaks) 27(2.84%), Yeon(淵) 23(2.42%) and Chun(川) and Tan(灘) 22(2.31%). Mostly, common landscape components of Gugok are entrusted natural things. It is expected that more studies about the analysis of characteristics of Gugok's positioning types considering total distance and a gradient are required to understand more clearly characteristics and location distribution of true Gugok and its landscape components.

• Journal of the Korean Institute of Traditional Landscape Architecture
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• v.34 no.2
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• pp.1-15
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• 2016

This study aims at the consideration of the usages of traditional waterscape elements, which are difficult to define their concepts and their differences and it has been proceeded mainly with analysis of literature. It elicited various waterscape types by extracting the place names associated with the watersacpe elements from the chapter "Sancheon" of the volumes Gyeongsang-province in "Sinjeung Donggukyeojiseungram", which is a government-compiled geography book in the early period of Joseon Dynasty, and drew the features of each waterscape element by interpreting the dictionary definition and the original text and studying the similar examples. The results of study are drawn as follows. 1. The chapter "Sancheon" includes 22 types of waterscape elements and they are classified by means of locations and water-flow forms: River Landscape, Lake & Pond Landscape, Coast landscape. 2. River landscape maintaining constant natural water-flow constitutes the linear type, related to the class of stream, which includes 'Su(water)', 'Gang(river)', 'Cheon(stream)' and 'Gye(brook)' and the dotty type, created by the nature of trenched meander rivers, which includes 'Tan(beach)', 'Roe(rapids)', 'Pok(waterfall)' and 'Jeo(sandbank)'. 3. Lake & Pond Landscape forming water collected in a certain area constitutes 'Ho(lake)', which is a broad and calm spot created around mid and down stream of river, 'Yeon(pool)', 'Dam(pond)', 'Chu(small pond)', which are naturally created on the water path around mid and down stream of river, 'Ji(pond)', 'Dang(pond)', 'Taek(swamp)', which is collected on a flatland and 'Cheon(spring)', 'Jeong(spring)' which means gushing out naturally. 4. Coast Landscape includes 'Ryang', 'Hang', which are the space between land and an island or islands, 'Got(headland)' which sticks out from the coast into the sea, 'Jeong(sandbank)' which forms sandy beaches and 'Do' which shows high appearance frequency by reflecting the geographical importance of islands. This study comprehended the diversity of traditional waterscape elements and drew the fact that they are the concept reflecting the differentiated locational, scenic and functional features. That way, it understood the aesthetic sense on nature, which ancestors had formed with the interests in natural landscape and the keen observation on it, became the basic idea elucidating the characteristic on Korean traditional gardens, which minimize the artificiality and make nature the subject.

• Korean Journal of Poultry Science
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• v.46 no.1
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• pp.31-37
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• 2019

A chicken clathrin-associated adaptor protein $3-{\delta}$ subunit 2 (AP3S2) is a subunit of AP3, which is involved in cargo protein trafficking to target membrane with clathrin-coated vesicles. AP3S2 may play a role in virus entry into host cells through clathrin-dependent endocytosis. AP3S2 is also known to participate in metabolic disease developments of progressions, such as liver fibrosis with hepatitis C virus infection and type 2 diabetes mellitus. Chicken AP3S2 (chAP3S2) gene was originally identified as one of the differentially expressed genes (DEGs) in chicken kidney which was fed with different calcium doses. This study aims to characterize the molecular characteristics, gene expression patterns, and transcriptional regulation of chAP3S2 in response to the stimulation of Toll-like receptor 3 (TLR3) to understand the involvement of chAP3S2 in metabolic disease in chicken. As a result, the structure prediction of chAP3S2 gene revealed that the gene is highly conserved among AP3S2 orthologs from other species. Evolutionarily, it was suggested that chAP3S2 is relatively closely related to zebrafish, and fairly far from mammal AP3S2. The transcriptional profile revealed that chAP3S2 gene was highly expressed in chicken lung and spleen tissues, and under the stimulation of poly (I:C), the chAP3S2 expression was down-regulated in DF-1 cells (P<0.05). However, the presence of the transcriptional inhibitors, BAY 11-7085 (Bay) as an inhibitor for nuclear factor ${\kappa}B$ ($NF{\kappa}B$) or Tanshinone IIA (Tan-II) as an inhibitor for activated protein 1 (AP-1), did not affect the expressional level of chAP3S2, suggesting that these transcription factors might be dispensable for TLR3 mediated repression. These results suggest that chAP3S2 gene may play a significant role against viral infection and be involved in TLR3 signaling pathway. Further study about the transcriptional regulation of chAP3S2 in TLR3 pathways and the mechanism of chAP3S2 upon virus entry shall be needed.