• Korean Journal of Pharmacognosy
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• v.38 no.3 s.150
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• pp.287-290
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• 2007

Phytochemical investigation of the MeOH extract of the dried stem barks of Fraxinus rhynchophylla Hance (Oleaceae), as guided by cytoprotective activity against tert-butyl hydroperoxide (t-BHP)-induced cell injury in mouse hippocampal HT22 cells, furnished two coumarins, esculetin (1) and fraxetin (2). Compounds 1 and 2 had the significant cytoprotective effects on t-BHP-induced cellular oxidative injury in HT22 cells. Furthermore, compounds 1 and 2 showed potent DPPH radical scavenging effect, exhibiting $IC_{50}$ values of 14.68 and 9.64 ${\mu}M$, respectively.

• Asian-Australasian Journal of Animal Sciences
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• v.17 no.2
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• pp.259-262
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• 2004

The ability of Modified Glucomannan (MG) to bind T-2 toxin (T-2) in the gastrointestinal tract has been tested in vivo by feeding 120 five-wk-old broiler chicken with the following six treatment diets, 1) Control diet; 2) Control+MG (0.1%); 3) Control+T-2 (500 ppb); 4) Control+T-2 (500 ppb)+MG (0.1%); 5) Control+T-2 (1,000 ppb) and 6) Control+T-2 (1,000 ppb)+MG (0.1%). Twenty birds were assigned to each treatment group, which had five experimental groups. Four birds of each experimental group were sacrificed at an interval of 30 min i.e. at 0, 30, 60, 90 and 120 min after feeding experimental diets. The whole gut contents of each bird were collected, dried and toxin concentration was determined. Percent T-2 recovered from the gut was significantly lower (p<0.05) in the groups fed MG at all the time intervals. The percent T-2 adsorbed by the MG at different T-2 levels (500 and 1,000 ppb) was 15.97 and 14.77, 22.53 and 22.67, 26.88 and 28.03, and 31.50 and 31.83 at 30, 60, 90 and 120 min, respectively.

• Korean Journal of Microbiology
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• v.55 no.3
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• pp.234-241
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• 2019

An intensive interaction between yeasts and insects has highlighted their relevance for attraction to food and for the insect's development and behavior. Yeast associated in the gut of insects secretes cellulase which aided in the food digestion (cellulose degradation). Three strains of cellulose-degrading yeast were isolated from the gut of adult grasshoppers collected in Gyeonggi Province, South Korea. The strains $ON22^T$, $G10^T$, and $G15^T$, showed positive cellulolytic activity in the carboxymethyl cellulose (CMC)-plate assay. The phylogenetic tree based on sequence analysis of D1/D2 domains of the large subunit rRNA gene and the internal transcribed spacer (ITS) regions revealed that the strains $ON22^T$ (100 and 98.4% sequence similarities in D1/D2 domains and ITS) and $G10^T$ (99.8 and 99.5% in D1/D2 domain and ITS region) were most closely related to the species Moesziomyces aphidis JCM $10318^T$; $G15^T$ (100% in D1/D2 domains and ITS) belongs to the species Moesziomyces antarcticus JCM $10317^T$, respectively. Morphology and biochemical test results are provided in the species description. Cellulase with its massive applicability has been used in various industrial processes such as biofuels like bioethanol productions. Therefore, this is the first report of the cellulolytic yeast strains $ON22^T$, $G10^T$, and $G15^T$ related to the genus Moesziomyces in the family Ustilaginaceae (Ustilaginales), in Korea.

• The Journal of Korean Academic Society of Nursing Education
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• v.22 no.4
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• pp.567-576
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• 2016

Purpose: This study was conducted to test the effects of flipped learning for undergraduate nursing students. Methods: The study was a nonequivalent control group pretest-posttest design. Participants were 140 nursing students (experimental group=70, control group=70). The flipped learning was provided for 6 times (18 hours). Data were collected between March 7 and May 30, 2016. Data were analyzed using Chi-square, t-test, paired t-test with SPSS/WIN 22.0. Results: The results showed that self-efficacy (t=4.67, p<.001), critical thinking disposition (t=6.55, p<.001), and communication competence (t=3.57, p=.001) were significantly higher than in the experimental group than in the control group. Conclusion: The study findings suggest that the flipped learning has proven to be influential educational program for improving the self-efficacy, critical thinking disposition, and communication competence of nursing students. The flipped learning was an effective teaching program for nursing student, and need to develop other nursing major subjects.

• Molecules and Cells
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• v.27 no.5
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• pp.557-562
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• 2009

We examined the effects of the NADPH oxidase p22phox C242T polymorphism on endurance exercise performance and oxidative DNA damage in response to acute and chronic exercises. One hundred three subjects were recruited, among which 26 healthy subjects (CC: 12, TC: 12, and TT: 2) were studied during rest, exercise at 85% $VO_2max$, and recovery before and after 8 weeks of treadmill running. Lymphocyte DNA damage increased significantly in response to exercise (p < 0.05). There were no significant differences in plasma MDA, SOD concentrations and lymphocyte DNA damage between CC genotype and T allele group, but significant endurance training differences were observed. Endurance training increased exercise time to exhaustion in both the CC genotype and T allele groups (p < 0.05) but no significant difference was found between groups. The results of the current study with young, healthy, Korean men are interpreted to mean that 1) the majority had the CC genotype of the NADPH oxidase p22phox C242T polymorphism (82.5%: CC, 15.5%: TC, 1.9%: TT), 2) acute exercise increased lymphocyte DNA damage, 3) endurance training significantly increased exercise time to exhaustion, and alleviated lymphocyte DNA damage, and 4) The NADPH oxidase p22phox C242T polymorphism, however, did not alter lymphocyte DNA damage or exercise performance at rest, immediately after exercise, or during recovery.

• Journal of Korean Neurosurgical Society
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• v.59 no.6
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• pp.615-621
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• 2016

Objectives : To review surgical results of post-fusion lumbar flatback treated with pedicle subtraction osteotomy (PSO) or Smith-Petersen osteotomies (SPOs). Methods : Twenty-eight patients underwent osteotomies. Radiological outcomes by sagittal vertical axis (SVA), and pelvic tilt (PT), T1 pelvic angle (T1PA), and pelvic incidence (PI)-lumbar lordosis (LL) at preoperative, postoperative 1 month, and final were evaluated. Oswestry Disability Index (ODI), visual analog scale (VAS) score of back pain/leg pain, and Scoliosis Research Society-22 score (SRS-22r) were analyzed and compared. Patients were divided into 2 groups (SVA ${\leq}5cm$ : normal, SVA >5 cm : positive) at final and compared outcomes. Results : Nineteen patients (68%) had PSO and the other 9 patients had SPOs with anterior lumbar interbody fusions (ALIFs) (Mean age : 65 years, follow-up : 31 months). The PT, PI-LL, SVA, T1PA were significantly improved at 1 month and at final (p<0.01). VAS score, ODI, and SRS-22r were also significantly improved at the final (p<0.01). 23 patients were restored with normal SVA and the rest 5 patients demonstrated to positive SVA. SVA and T1PA at 1 month and SVA, PI-LL, and T1PA at final were significantly different (p<0.05) while the ODI, VAS, and SRS-22r did not differ significantly between the groups (p>0.05). Common reoperations were early 4 proximal junctional failures (14%) and late four rod fractures. Conclusion : Our results demonstrate that PSO and SPOs with ALIFs at the lower lumbar are significantly improves sagittal balance. For maintenance of normal SVA, PI-LL might be made negative value and T1PA might be less than $11^{\circ}$ even though positive SVA group was also significantly improved clinical outcomes.

• Aerospace Industry
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• v.79
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• pp.22-22
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• 2002

• Journal of the Korea Society of Computer and Information
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• v.22 no.9
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• pp.91-97
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• 2017

IoT (Internet of Things) systems are based on heterogeneous hardware systems of different types of devices interconnected each other, ranging from miniaturized and low-power wireless sensor node to cloud servers. These IoT systems composed of heterogeneous hardware utilize data sets collected from a particular set of sensors or control designated actuators when needed using open APIs created through abstraction of devices' resources associated to service applications. However, previously existing IoT services have been usually developed based on vertical platforms, whose sharing and exchange of data is limited within each industry domain, for example, healthcare. Such problem is called 'data silo', and considered one of crucial issues to be solved for the success of establishing IoT ecosystems. Also, IoT services may need to dynamically organize their services according to the change of status of connected devices due to their mobility and dynamic network connectivity. We propose a way of dynamically composing IoT services under the concept of WoT (Web of Things) where heterogeneous devices across different industries are fully integrated into the Web. Our approach allows developers to create IoT services or mash them up in an efficient way using Web objects registered into multiple standardized horizontal IoT platforms where their resources are discoverable and accessible. A Web-based service composition tool is developed to evaluate the practical feasibility of our approach under real-world service development.

• Journal of Microbiology and Biotechnology
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• v.27 no.3
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• pp.535-541
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• 2017

Two Gram-staining-negative, red-pinkish, coccus-shaped, non-motile, and aerobic bacterial strains, designated $Ant21^T$ and Ant22, were isolated from the Antarctic coastal sea water. Strains $Ant21^T$ and Ant22 showed UVC and gamma radiation resistance. Phylogenetic analyses based on 16S rRNA gene sequences determined that these strains belong to the genus Deinococcus. Through the analyses of the 16S rRNA gene sequences, strains $Ant21^T$ and Ant22 were found to have 97.7% and 97.8% similarity to Deinococcus marmoris DSM $12784^T$ and 97.0% and 97.2% similarity to Deinococcus saxicola AA-$1444^T$, respectively. The sequence similarity with the type strains of other Deinococcus species was less than 96.9% for both strains. Strains $Ant21^T$ and Ant22 shared relatively high 16S rRNA gene sequence similarity (99.3%) and had a closely related DNA reassociation value of $84{\pm}0.5%$. Meanwhile, they showed a low level of DNA-DNA hybridization (<30%) with other closely related species of the genus Deinococcus. The two strains also showed typical chemotaxonomic features for the genus Deinococcus, in terms of the major polar lipid (phosphoglycolipid) and the major fatty acids ($C_{16:0}$, $C_{16:1}$ ${\omega}6c/{\omega}7c$, $iso-C_{17:0}$, and $iso-C_{15:0}$). They grew at temperatures between $4^{\circ}C$ and $30^{\circ}C$ and at pH values of 6.0-8.0. Based on the physiological characteristics, the 16S rRNA gene sequence analysis results, and the low DNA-DNA reassociation level with Deionococcus marmoris, strains $Ant21^T$ ($=KEMB\;9004-167^T$ $=JCM\;31436^T$) and Ant22 (KEMB 9004-168 =JCM 31437) represent novel species belonging to the genus Deinococcus, for which the name Deinococcus rubrus is proposed.

• Korean Journal of Microbiology
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• v.46 no.3
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• pp.233-239
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• 2010

Four genes (yqfR, yfmL, ydbR, deaD) were identified as putative DEAD-box RNA helicase genes in the genomic sequence of Bacillus subtilis by homology search. To understand the function of these genes, each of the genes was deleted and the constructed strains were tested for their growth charateristics at different temperatures. The growth rate of ydbR deletion mutant ($T_d$=53 min) was a little bit reduced at $37^{\circ}C$ as compared to that of wild type strain (CU1065). But the growth rate of other three (yqfR, yfmL, deaD) deletion mutants ($T_d$=30-40 min) is nearly equal to the growth rate of wild type ($T_d$=32 min). On the other hands, the growth rate of deletion mutants were reduced at $22^{\circ}C$ in order of yqfR ($T_d$=151 min), yfmL ($T_d$=214 min), ydbR ($T_d$=343 min), which showed cold-sensitive phenotype. The deletion mutant of deaD ($T_d$=109 min) grew equally as compared to the growth rate ($T_d$=102 min) of the wild type at $22^{\circ}C$ and did not show cold-sensitive growth. Double, triple and quadruple deletion mutants of these genes were constructed, and growth rate of these mutants were measured at various temperature conditions ($22^{\circ}C$, $37^{\circ}C$, $42^{\circ}C$) using LB broth. Multiple deletion mutations showed more severe cold-sensitive growth than single deletion mutations, and double deletion of ydbR and yfmL ($T_d$=984 min) showed most cold-sensitive growth than any other double mutants. Such a cold-sensitive growth of these mutations is quite similar to the result of csdA or srmB deletion in E. coli and suggested that physiological role of ydbR and yfmL is related with ribosome assembly.