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Purification Nematicidal Substance and Nematicidal Activity from Ginkgo biloba L. Outer Seedcoat (은행 외종피로부터 살선충 물질의 순수 분리와 활성)

  • Jang, Yu Ju;Hwang, Hyeon Jeong;Kim, Keun Ki
    • Korean Journal of Organic Agriculture
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    • v.29 no.1
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    • pp.97-109
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    • 2021
  • Plant parasitic nematodes are causing significant damage in crop production. There is a need to develop eco-friendly nematicide that reduces the damage of nematode and has little effect on the environment and human. In this study, we have isolated a substance having nematicidal activity from Ginkgo biloba L. outer seedcoat. Studies of G. biloba L. outer seedcoat are insufficient compared to the seed and leaves due to their odor and toxicity. The dried G. biloba L. outer seedcoat was extracted with dichloromethane:methanol (1:1) and fractionated into hexane, ethyl acetate and H2O. Four steps TLC were performed from EtOAc fraction to purely isolate GB4-3 with nematicidal activity. To compare nematicidal activity, G. biloba L. seedcoat methanol extract and purified GB4-3 were investigated in terms of treatment concentration and time. As a result, the nematicidal activity increased with concentration and time. In the place treated with 20 ㎍/mL of crude G. biloba L. seedcoat MeOH extract, strong activity appeared after 12 hours, and 46% nematicidal activity shown after 18 hours. About 69% of nematicidal activity was confirmed in the place where GB4-3 purified from outer seedcoat was treated with 20 ㎍/mL, and the possibility of development as nematicide was very high. This study could be used as a basic data for the development of a nematode preparation from G. biloba L. outer seedcoat.

Influence of El Niño on the Production of Highland Kimchi Cabbage in South Korea (국내 고랭지배추 생산량에 대한 엘니뇨 영향)

  • Shim, Kyo-Moon;Kim, Yongseok;Hur, Jina;Jo, Sera;Kang, Kee-Kyung
    • Korean Journal of Agricultural and Forest Meteorology
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    • v.22 no.4
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    • pp.279-286
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    • 2020
  • The objective of this study was to assess the impact of El Niño on highland kimchi cabbage production for the period from 1991-2016 in South Korea. Years with less than 1.0 Oceanic Niño index (ONI) were classified into non El Niño years, while years with equal to or greater than 1.0 ONI were defined as El Niño years. The national average production (3,444 kg 10a-1) of high kimchi cabbage in El Niño years tended to be less than that in non El Niño years (3,722 kg 10a-1) with significant differences (p = 0.0042) in the production between these groups of years. The averaged production of highland kimchi cabbage of El Niño end years (3,289 kg 10a-1) was less than those of El Niño start years and non El Niño years by 310 and 433 kg 10a-1, respectively. Such difference was significant statistically (p=0.035). According to our analysis, the differences in kimchi cabbage productions resulted from low temperature, short sunshine duration, and precipitation increase during the cultivation period of highland kimchi cabbage. This study may help for further analysis on the impact of extreme weather conditions during El Niño years on crop production.

Effects of the Structured Nursing Intervention Program on Dysmenorrhea of Middle School Girls (구조화된 간호중재 프로그램이 여중생의 월경곤란증 완화에 미치는 영향)

  • Choi, Soung-Hee;Kim, Hyeon Ok
    • Journal of the Korean Society of School Health
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    • v.14 no.1
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    • pp.145-160
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    • 2001
  • The main purpose of this study is to identify the effects of a structured nursing intervention program on dysmenorrhea in middle school girls. This study employed two methods of research at the same time: a quasi-experiment pre- and post-test to use for comparison. Subjects were middle school girls assigned to the research program: 40 girls in the second grade, 40 girls in the third grade at K Girls' Middle School and H Girls' Middle School located in J city. The data were collected for 68 days from August 23 to October 30, 1999. The data were collected by observation technique and by a self-administered questionnaire. The instruments used for this study were as follows: the "Face Interval Pain Scale" menstrual pain instrument developed by McGreth(1987) and the "Menstrual Distress Questionnaire" dysmenorrhea instrument developed by Moos(1968). The dysmenorrhea knowledge instrument was developed by the researcher and refered to above. The research procedure was as follows: after preliminary examination, the experimental groups were provided educational programs with O.H.P. films developed by the researcher and with the videotape "first meeting" supported by P&G company and produced by EBS. The groups followed the educational programs twice for two weeks. At the beginning of menstruation, these subjects could choose one among the following choices: * Jacobson's progressively muscle relaxation method and massage treatment * Jacobson's progressively muscle relaxation method and hot bag treatment * Jacobson's progressively muscle relaxation method, a pain killer treatment, and massage treatment * Jacobson's progressively muscle relaxation method, a pain killer treatment, and hot bag treatment The dysmenorrhea of the control group were measured during the same period. The analysis of the collected data was done using an SPSS-PC+, descriptive statistic including real numbers, percentage, averages, standard deviations and t-test, $x^2$ test, and ANCOVA. The results of this study were as follows. (1) The knowledge level of the girls provided with the planned nursing intervention program was higher when compared to these who did not follow the educational programs. (2) The original difference in the dysmenorrhea level having been controled, the dysmenorrhea level made, nevertheless, a statistical difference between the experimental group and the control group(F=63.8, p=.00). Observed pain on quantity measurement of dysmenorrhea showed significant difference depending on the treatment(t=4.6, p=.00). In conclusion, those in the planned nursing intervention program had a higher knowledge level and fewer symptoms than the control group. Accordingly, the nursing intervention program for dysmenorrhea developed by the researcher can be effectively applied in nursing practice and can be the foundation for this kind of program.

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Flying Aphid Population at the Horticultural Experiment Station, Suweon (원예시험장 주변의 진딧물)

  • Paik Woon Hah;Song Ki Won;Choi Seong Sik
    • Korean journal of applied entomology
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    • v.13 no.1 s.18
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    • pp.25-31
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    • 1974
  • This survey was aimed to accumulate basic data of aphid population at the Horticultural Experiment Station at Suweon. The yellow pan traps were setted at five locations (Fig.1.), and ran from May 1 to October 31. 1970. About one hundred and twenty species of aphids were trapped, including 24 species of plant vims vectors. Of these, dominant species were as follows: (Asterisk shows virus vector) Aphid species No. of catches * Aphis spiraecola PATCH 2,635, * Aphis craccivora KOCH 2,377, * Myzus persicae SULXER 2,111, Capitophorus hippophaes javanicus H.R. LAMBERS 2,051, Anoecia fulviabdominalis SASAKI 1,480, * Aphis gossypii GLOVER 867, * Macrosiphum avenae FABRICIUS 859, Cervaphis quercus TAKAHASHI 692, * Lipaphis erysimi KALTENBACH 645, Pleotrichophorus chrysanthemi THEOBALD 489, The above 10 species consisted $76.5\%$ of total catches and the 24 vector species consisted $55.5\%$. The curve of the seasonal occurrence of flying aphids at Horticultural Experiment Station shows bimodal, typical for the temperate region. The total number of trapped aphids at the Station from May to September, 1970, were less than that of average yearly catches at the College of Agriculture from 1967 to 1970. Thi, low numbers at Horticultural Experiment Station may attribute to the frequent spraying of insecticides from Spring to Summer on growing crops there. But the aphids population increase suddenly in the middle of October. This might be resulted from cease of insecticide applications and migration of aphids from summer host to winter host plants.

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Production and High Temperature Mechanical Properties of Ti-TiC Composite by Reaction Milling (반응밀링법에 의한 Ti-TiC 복합재료의 제조 및 고온 기계적 특성)

  • Jin, Sang-Bok;Choe, Cheol-Jin;Lee, Sang-Yun;Lee, Jun-Hui;Kim, Sun-Guk
    • Korean Journal of Materials Research
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    • v.8 no.10
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    • pp.918-924
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    • 1998
  • This study has been carried out to investigate the effect of reaction milling time on the synthesis of Ti- TiC p powder synthesised from the elemental titanium and activated carbon by reaction milling(RM), and the effect of vacu­u urn hot pressing temperature and TiC volume fraction on microstructural and mechanical properties of Ti- TiC com­p posite $\infty$ns이idated by vacuum hot pressing(VHP).T The elemental powders of titanium and activated carbon can be converted into Ti- TiC composite powders by reac­t tion milling for about 300hours, and were the average grain size of the as- milled powders has been measured to be a about $5\mu\textrm{m}$. The relative density of Ti- TiC VHPed above $1000^{\circ}C$ during Ihr is about 98% and the mechanical properties o of In- situ Ti- TiC composites are improved by TiC particle dispersed uniformly on titanium matrix. In order to investi­g gate thermal stability of Ti- TiC composite, after annealing at $600^{\circ}C$ for 80hrs micro- Vickers hardness have been per­f formed, and the values have been shown little changed as compared with those before annealing. The compact has b been tested on high temperature compressive test at $700^{\circ}C$ and has showed a high temperature compressive strength of 330MPa in a Ti- 20vol% TiC.

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The Comparison Consideration on Experimental Articles about Melanoma Published in Journals of Korean Medicine (국내 한의학계에 보고된 흑색종 관련 실험적 연구들의 비교 고찰)

  • Kwon, Kang;Kim, Nam-Kwen;Kim, Sung-Young;Lee, Dong-Jin;Kim, Chul-Yun;Seo, Hyung-Sik
    • The Journal of Korean Medicine Ophthalmology and Otolaryngology and Dermatology
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    • v.28 no.3
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    • pp.30-47
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    • 2015
  • Objective : Melanoma is a very critical and devastating disease. Although many people have depended on surgical operation in melanoma treatment, they have placed importance on non-invasive methods constantly. So we planned to establish a research methodology by analysing existing articles containing conservative melanoma treatments in Journals of Korean Medicine published in Korea.Methods : Using search words of anti-cancer, B16, cancer, lung metastasis, melanoma, metastasis, S-100, SK-MEL, tumor, tyrosinase, we collected 26 articles by searching internet portal sites as following;Using search words of anti-cancer, B16, cancer, lung metastasis, melanoma, metastasis, S-100, SK-MEL, tumor, tyrosinase, we collected 26 articles by searching internet portal sites as followinghttp://oasis.kiom.re.kr,http://www.koreantk.com,http://www.riss.kr,http://www.dbpia.co.kr,http://www.ndsl.kr,http://kiss.kstudy.com,http://www.naver.com,http://www.google.com.Result : The number of articles is 26 and in the year of 2003, 2004 is ranked the highest number in publication. The journal of acupuncture & moxibustion society ranked the highest(30.8%). 2 and 4 authors ranked the highest(26.9%) in number of authors. T-test ranked the highest(58.1%) in statistics methods. P.O. med indicated in 11 articles and Pharmacopuncture in 15 articles. B16 murine melanoma cell was indicated in 25 articles by cancer-induced methods. In measurement, T cell activity was indicated in 14 articles, NK activity in 4 articles, IL-2 in 6 articles, apoptosis in 1 article, lung metastasis in 14 articles.Conclusion : Considering overall results, it is necessary to diversify cancer-induced methods and measurement methods in experimental melanoma research.

Effect of Light-emitting Diodes on Photosynthesis and Growth of in vitro Propagation in Tea Tree (Camellia sinensis L.) (LED 광질이 차나무 기내배양묘의 생육 및 광합성에 미치는 영향)

  • Im, Hyeon-Jeong;Na, Chae-Sun;Song, Chi-Hyeon;Won, Chang-O;Song, Ki-Seon;Hwang, Jung-Gyu;Kim, Do-Hyun;Kim, Sang-Geun;Kim, Hyun-Chul
    • Journal of agriculture & life science
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    • v.53 no.6
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    • pp.13-21
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    • 2019
  • The influences of light generated by LEDs on shoot growth and photosynthesis of Tea plant(Camellia sinensis L.) were evaluated. The growth characteristics were investigated after 45 days of culture under four different light qualities: fluorescent lamp, red LED, blue LED, red+blue+white LED. Shoot growth was promoted by red light, especially root length and area were further promoted under the red+blue+white LED. Also, T/R ratio and Chlorophyll content were highest in red+blue+white. Fluor Cam was used to measure the fluorescence images of the plants, inhibition of photochemical efficiency(Fv/Fm) were not changed in all treatment. However, non-photochemical quenching(NPQ) were found rapidly increasing in blue LED, these results were that blue LED were inhibit photosynthetic efficiency and must be considered for efficiently in vitro cultivation of the tea plant. The above results suggest that light qualities could be an important factor to foster in vitro growth of the species. Also, In order to produce healthy plants, it is effective to using light qualities of red+blue+white LED on in vitro culture of the tea plant. These results could be used to mass propagating shoot and produce of healthy seedling.

Changes in Dormant Phase and Bud Development of 'Fuji' Apple Trees in the Chungju Area of Korea (충주지역에서 '후지' 사과나무의 휴면단계 변화 및 눈 발달)

  • Lee, ByulHaNa;Park, YoSup;Park, Hee-Seung
    • Horticultural Science & Technology
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    • v.33 no.4
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    • pp.501-510
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    • 2015
  • In this study, we investigated the onset and release of endo-dormancy under natural conditions by observing bud break characteristics in 'Fuji' apple trees using water cuttings. Through examinations of bud break rate and days to bud break, we found that the endo-dormancy of 'Fuji' apple tree continues for 70 d from 165 to 255 d after full bloom (DAFB), from late October to early January of the following year. In addition, within 20 d of first bud break, based on a final bud break rate of 60% or more, we able to identify the timing of the changeover from para-dormancy to endo-dormancy, and endo-dormancy to eco-dormancy. Analysis of the chilling requirement during the endo-dormancy period revealed that chilling accumulation up to 255 DAFB to release endo-dormancy amounted to 666 and 517 h based on the CH and Utah models, respectively. Observation of internal changes in the bud during endo-dormancy showed that flower bud differentiation begins from mid-July, and t ime of inflorescence o f the disk f lower is a vailable to f ind. The f lower buds subsequently developed slowly but steadily during endo-dormancy and in the following year in February, the developmental stage of each organ had progressed. Moreover, the flower buds of 'Fuji' apples were mostly healthy during the dormancy period, but some exhibited necrosis of flower primordium, due partial cell damage from the formation of ice crystals rather than a direct effect of the low temperature. Flower buds were formed in both the axillary buds of bourse shoots and terminal buds of spurs, but lower bud differentiation was observed for the terminal buds of spurs at rate of about 65% of total buds, which was directly related to the bud size and shoot diameter.

Comparison of the Legislation Applicable to Compare the use of Diagnostic Radiation Devices (진단용 방사선발생장치 이용에 적용되는 법제의 비교)

  • Ko, Jong-Kyung;Jeon, Yeo-Ryeong;Han, Eun-Ok;Cho, Pyong-Kon;Kim, Yong-Min
    • Journal of radiological science and technology
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    • v.38 no.3
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    • pp.277-286
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    • 2015
  • Diagnostic radiation devices that is used in the country has reached to 78,000 units. When used for human subjects diagnostic purposes, it is subject to Medical Service Act, when used in diagnostic purposes in animal subjects, the subject to Veterinarians Act. When used for other purposes are subject to the Nuclear Safety Act. Even the same radiation devices varies the legislation that is applied depending on the intended use and object. Diversified been p rovisions a re necessary compared to t he analysis o f l egal content in o rder t o prevent confusion of the legislation is a matter to be applied. It is a qualitative study that Nuclear Safety Act, Medical Service Act and Veterinarians Act administrative procedures for the introduction of the applied diagnostic radiation devices, safety inspection, human resources management, area management and the content related to administrative punishment. The Nuclear Safety Act sub-provisions, the introduction of diagnostic radiation generating devices, there are many complex and complete requirements administrative procedures on the concept of a permit. Inspection of safety associated with the use, would be subject to periodic inspection auditing characteristics over the entire field of radiation safety management. It must receive court regular education for the safety administrator and workers. Unlike the reference of the radiation dose rate to specify the radiation controlled area there is a measurement obligation of radiation dose rate. Unlike the reference of the radiation dose rate to specify the radiation controlled area there is a measurement obligation of radiation dose rate. Quantitative difference of administrative punishment that is imposed when legislation violation has reached up to 10 times, over the entire field, the largest burden of radiation safety management at the time of application of the Nuclear Safety Act sub provisions. And it is applied differently depending on the purpose and the imaging target using the same diagnostic radiation devices. Depending on the use mainly under the current legal system, radiation can be lacking in fairness of the contents of the legislation for safety management, there is a risk of confusion. Alternatives such as centralized and standardization of legislation by diagnostic radiation devices use is expected to be necessary.

An Analysis on Changing Pattern of Economic Active Population by Working Life Table for Korean Men (우리나라 노동생명표에 의한 노동력추이 분석)

  • 조진만
    • Korea journal of population studies
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    • v.13 no.2
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    • pp.1-18
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    • 1990
  • This is a study which attempt to analyze changing patierns of economic active popu-lation, t o estimato- future patterns, and exa- mine vartons problems arises by changing c ire u mst ances of t he labor force market in- clunging soici al, economic ic, heathl th and demoi-graph ic aspects. We have constructed series of wotking life table which are useful in syt uiolyioig the lirocess of growth and structural change of labor force. Work i ng life tables represent ihie life eyele of econrmic' activity in hi ypothetical cohorts, that is. gen-erat i on of men Sn bject at eat' b period ot f their lives th given ra to's o mor tali it y and of par-- tici pation in economic activities. The tabloes prot' ide measorues of the alvet'age he ng t able of economically aeti \- e life. and agespecific rates of en trannee' into and retirement from the hahn' force. In const routing working life tables, age-specific activity rates and life tabole popula- titoto which represents contemporary condi-tions of moortality in Korea au'e the basic' maltoerials. We have derived the age-specific rates foorm economically active population survey, whoich were conducted by the Bureau of Statistics, Economic Planning Borard of the Korean government. Working life tables are constructed for men wtable these materi- als in the year of 1970, 1980 and 1988 by a modified Wolfbein-Wool's method. Some of the findings may be summerized as follow : 1) A central part of constructing working life table is calculation of stationary' economic active population, which represents the number of men in the stationtary population extoected to be in the labor force at each age group in the life span. The stationary economic active population by age have generally a universal pattern, where they rise sharply in the early twenties, approach its' peak in the thirties decline thereafter. at first graolually and then more rapidly at an advanced age. Korean men show the same general pa ttern of age distribution of stationary eco-- nomic active population with sharp increase hegining from the age interval of 20 to 24, reaching to maximum level at older age. The population. however, presumably, increased substantially due to increaseing school atte endance rates. Another difference exiSts in the youngest age groups, that is the activity rate in the year of 1988 is lower than that of Japan. The table shows an analysis of changes in the age distrihution of labor force between 1970, 1980 and 1988. 2) It was shown an analysis of changes in the age distribution and cause of separation from labor force. The entrance rate to labor force has increased from 18~\5 persons to 299 persons per 1000 head of stationary population between that of 1980 than that of 1988 for Korean men in 20~24 age group. The entrace rate to labor force shows a rapid entrance appearance concentrated on the 15~24 age group. The separation rate from labor force by retirment in Korea in the year of 1988 shows a great difference of the about four times as much as that of Japan. 3) The functions of table illustrate the patterns of working life of males in Korea in 1970, 1980 and 1988. The average remaining number of economically active years, e at age 15 in 1988 is 46.39 which is 2.12 years of increase compared with that of at age 15 in 1970,1980 and 1988 are 43.90,44.27 and 46.39 respectively, showing steadily increase dur- ing the past double decade the increase in the length of economically active life various age may be considered to have come both from extention of general life expectancy and from increasing entrance rate to economic activity in high age that of working is far greater in 1988 than that of 1980. The gaps between expectation of life and average remaining years of economically active widened due to rapid improvement of mortality level in Ko- rea. This observation together with the population pressure by the appearance of a group of younger population implies that constant increase of economically inactive population among older age group.

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