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http://dx.doi.org/10.11625/KJOA.2021.29.1.97

Purification Nematicidal Substance and Nematicidal Activity from Ginkgo biloba L. Outer Seedcoat  

Jang, Yu Ju (고려대학교 생명과학대학 식물생명공학과 대학원)
Hwang, Hyeon Jeong (부산대학교 생명자원과학대학 생명환경화학과)
Kim, Keun Ki (부산대학교 생명환경화학과, 생명산업융합연구원)
Publication Information
Korean Journal of Organic Agriculture / v.29, no.1, 2021 , pp. 97-109 More about this Journal
Abstract
Plant parasitic nematodes are causing significant damage in crop production. There is a need to develop eco-friendly nematicide that reduces the damage of nematode and has little effect on the environment and human. In this study, we have isolated a substance having nematicidal activity from Ginkgo biloba L. outer seedcoat. Studies of G. biloba L. outer seedcoat are insufficient compared to the seed and leaves due to their odor and toxicity. The dried G. biloba L. outer seedcoat was extracted with dichloromethane:methanol (1:1) and fractionated into hexane, ethyl acetate and H2O. Four steps TLC were performed from EtOAc fraction to purely isolate GB4-3 with nematicidal activity. To compare nematicidal activity, G. biloba L. seedcoat methanol extract and purified GB4-3 were investigated in terms of treatment concentration and time. As a result, the nematicidal activity increased with concentration and time. In the place treated with 20 ㎍/mL of crude G. biloba L. seedcoat MeOH extract, strong activity appeared after 12 hours, and 46% nematicidal activity shown after 18 hours. About 69% of nematicidal activity was confirmed in the place where GB4-3 purified from outer seedcoat was treated with 20 ㎍/mL, and the possibility of development as nematicide was very high. This study could be used as a basic data for the development of a nematode preparation from G. biloba L. outer seedcoat.
Keywords
ginkgo; isolation; nematicide; nematode; outer seedcoat;
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