• Journal of Microbiology and Biotechnology
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• 제16권10호
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• pp.1529-1536
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• 2006

We cloned a gene of ompH(D:4) from pigs infected with P. multocida D:4 in Korea [16]. The gene is composed of 1,026 nucleotides coding 342 amino acids (aa) with a signal peptide of 20 aa (GenBank accession number AY603962). In this study, we analyzed the ability of the ompH(D:4) to induce protective immunity against a wild-type challenge in mice. To determine appropriate epitope(s) of the gene, one full and three different types of truncated genes of the ompH(D:4) were constructed by PCR using pET32a or pRSET B as vectors. They were named ompH(D:4)-F (1,026 bp [1-1026] encoding 342 aa), ompH(D:4)-t1 (693 bp [55-747] encoding 231 aa), ompH(D:4)-t2 (561 bp [187-747] encoding 187 aa), and ompH(D:4)-t3 (540 bp [487-1026] encoding 180 aa), respectively. The genes were successfully expressed in Escherichia coli BL21(DE3). Their gene products, polypeptides, OmpH(D:4)-F, -t1, -t2, and -t3, were purified individually using nickel-nitrilotriacetic acid (Ni-NTA) affinity column chromatography. Their $M_rs$ were determined to be 54.6, 29, 24, and 23.2 kDa, respectively, using SDS-PAGE. Antisera against the four kinds of polypeptides were generated in mice for protective immunity analyses. Some $50{\mu}g$ of the four kinds of polypeptides were individually provided intraperitoneally with mice (n=20) as immunogens. The titer of post-immunized antiserum revealed that it grew remarkably compared with pre-antiserum. The lethal dose of the wild-type pathogen was determined at $10{\mu}l$ of live P. multocida D:4 through direct intraperitoneal (IP) injection, into post-immune mice (n=5, three times). Some thirty days later, the lethal dose ($10{\mu}l$) of live pathogen was challenged into the immunized mouse groups [OmpH(D:4)-F, -t1, -t2, and -t3; n=20 each, two times] as well as positive and negative control groups. As compared within samples, the OmpH(D:4)-F-immunized groups showed lower immune ability than the OmpH(D:4)-t1, -t2, and -t3. The results show that the truncated-OmpH(D:4)-t1, -t2, and -t3 can be used for an effective vaccine candidate against swine atrophic rhinitis caused by pathogenic P. multocida (D:4) isolated in Korea.

• 대한수학회지
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• 제45권4호
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• pp.1075-1087
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• 2008

We study a class of KMS-symmetric quantum Markovian semigroups on a quantum spin system ($\mathcal{A},{\tau},{\omega}$), where $\mathcal{A}$ is a quasi-local algebra, $\tau$ is a strongly continuous one parameter group of *-automorphisms of $\mathcal{A}$ and $\omega$ is a Gibbs state on $\mathcal{A}$. The semigroups can be considered as the extension of semi groups on the nontrivial abelian subalgebra. Let $\mathcal{H}$ be a Hilbert space corresponding to the GNS representation con structed from $\omega$. Using the general construction method of Dirichlet form developed in [8], we construct the symmetric Markovian semigroup $\{T_t\}{_t_\geq_0}$ on $\mathcal{H}$. The semigroup $\{T_t\}{_t_\geq_0}$ acts separately on two subspaces $\mathcal{H}_d$ and $\mathcal{H}_{od}$ of $\mathcal{H}$, where $\mathcal{H}_d$ is the diagonal subspace and $\mathcal{H}_{od}$ is the off-diagonal subspace, $\mathcal{H}=\mathcal{H}_d\;{\bigoplus}\;\mathcal{H}_{od}$. The restriction of the semigroup $\{T_t\}{_t_\geq_0}$ on $\mathcal{H}_d$ is Glauber dynamics, and for any ${\eta}{\in}\mathcal{H}_{od}$, $T_t{\eta}$, decays to zero exponentially fast as t approaches to the infinity.

• 미생물학회지
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• 제29권6호
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• pp.387-391
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• 1991

Methylobacterium extorquens AM1 growing on methanol as a sole source of carbon and energy was found to grow most rapidly (t$t_{d}$ =6h) at 30.deg.C in a mineral medium (pH 7.0) containing 0.5% (v/v) methanol which was agitated at 200 rpm (optimal cultivation condition). Cells grown under the optimal cultivation condition contained more spermidine, but less putrescine, than the cells grown on 2.5%(v/v) ( $t_{d}$ =8h ) or at 20.deg.C ( $t_{d}$ =8h ). Cells cultivated under the optimal condition was found to contain more spermidine than the cells grown at pH 6.0 (( $t_{d}$ =7h ) and pH 8.0 ($t_{d}$ =7.3h). the cells growing at the stationary phase under the optimal condition accumulated more spermine or putrescine than the cells growing at the same phase on 2.5%(v/v) methanol or at pH 6.0 and pH 8.0, respectively. M. extorquens AM1 grown in a medium agitated at 100 rpm ( $t_{d}$ =8.8h ) contained less spermidine and spermine than the cells grown under the optimal cultivation condition.

• 대한수학회보
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• 제44권4호
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• pp.731-742
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• 2007

Let X be a Banach space, $A:D(A){\subset}X{\rightarrow}X$ the generator of a compact $C_0-semigroup\;S(t):X{\rightarrow}X,\;t{\geq}0$, D a locally closed subset in X, and $f:(a,b){\times}C([-q,0];X){\rightarrow}X$ a function of Caratheodory type. The main result of this paper is that a necessary and sufficient condition in order that D be a viable domain of the semi linear differential equation of retarded type $$u#(t)=Au(t)+f(t,u_t),\;t{\in}[t_0,\;t_0+T],{u_t}_0={\phi}{\in}C([-q,0];X)$$ is the tangency condition $$\limits_{h{\downarrow}0}^{lim\;inf\;h^{-1}d(S(h)v(0)+hf(t,v);D)=0}$$ for almost every $t{\in}(a,b)$ and every $v{\in}C([-q,0];X)\;with\;v(0){\in}D$.

• 대한수학회지
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• 제51권4호
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• pp.791-815
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• 2014

We show amongst other that if $f,g:[a,b]{\rightarrow}\mathbb{C}$ are two functions of bounded variation and such that the Riemann-Stieltjes integral $\int_a^bf(t)dg(t)$ exists, then for any continuous functions $h:[a,b]{\rightarrow}\mathbb{C}$, the Riemann-Stieltjes integral $\int_{a}^{b}h(t)d(f(t)g(t))$ exists and $${\int}_a^bh(t)d(f(t)g(t))={\int}_a^bh(t)f(t)d(g(t))+{\int}_a^bh(t)g(t)d(f(t))$$. Using this identity we then provide sharp upper bounds for the quantity $$\|\int_a^bh(t)d(f(t)g(t))\|$$ and apply them for trapezoid and Ostrowski type inequalities. Some applications for continuous functions of selfadjoint operators on complex Hilbert spaces are given as well.

• 대한수학회논문집
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• 제9권4호
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• pp.819-823
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• 1994

If H is a Hilbert space, then an operator $T : D(T) \subset H \to H$ is said to be monotone if $$ (x-y, Tx-Ty) \geq 0$$ for any x, y in D(T). Many authors [1], [4] obtained the existence theorem for the equation $y = x + Tx$ for x, given an element y in H and a monotone operator T. On the other hand some iterative methods were applied to the approximations for the solution of the above equation [6], [8]. For example Bruck [2] obtained the iterative solution of the above equation with an explicit error estimate as follows.

• 수산해양기술연구
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• 제13권2호
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• pp.1-4
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• 1977

원뿔대 모양의 은대구 통발을 권양할 때의 류휴저항을 측정하고, 그로부터 모랫줄의 굵기,권양의 소요 순마력등을 추산하는 식을 유도했다. 1.통발의 권양속도 v(m/sec)와 류휴저항(수중중량 포함)T(kg)사이에는 대체로 다음과 같은 관계가 성립한다. (a)빈통발을 직립으로 권양할 때: T=$48v^{1\cdot0}$ $45^\circ$ 경사지게 권양할 때: T=$50v^{1\cdot1}$ (단 $0.3\leqq v \leqq1.3$) 이고,이들 사이에 유의차는 없다. (b)밑면에 천을 깐 통발을 직립으로 권양할 때: T=$120^{1\cdot1}$ $45^\circ$ 경사지게 권양할 때: T=98v$^{1=cdot1}$ (단 $0.3\leqq v \leqq0.8$) 2.통발의 유류저항의 최대치를 T=$120v^{1\cdot1}$ (단 $0.3\leqq v \leqq0.8$)이라 보고, 모릿줄로써 P.P.3년 로우프를 쓴다면 d(mm)는 대략 $d=72\frac{D}{H}v^{1\cdot1}$ 을 만족시켜야 한다. 3.권양의 순마력 P(ps)는 $P=\frac{75}{120}\frac{D}{H}v^{2\cdot1}$ 이상일 것이 요구된다.

• 한국자기학회:학술대회 개요집
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• 한국자기학회 2008년도 Asian Magnetics Conference
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• pp.4.2-4.2
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• 2008

• 대한수학회논문집
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• 제19권2호
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• pp.307-319
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• 2004

In this paper, we establish some new oscillation criteria for the functional differential equations of the form $\frac{d}{dt}$$\frac{1}{a_{n-1}(t)}$$\frac{d}{dt}(\frac{1}{{a_{n-2}(t)}\frac{d}{dt}(...(\frac{1}{a_1(t)}\frac{d}{dt}x(t))...)))^\alpha$ + $\delta[f_1(t,s[g_1(t)],\frac{d}{dt}x[h_1(t)])$ + $f_2(t,x[g_2(t)],\frac{d}{dt}x[h_2(t)])]=0$ via comparing it with some other functional differential equations whose oscillatory behavior is known.

• 한국자기학회지
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• 제4권1호
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• pp.52-55
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• 1994

HD 기체에 대하 수소 및 중수소 원자핵 스핀-격자 완화시간 $T_{1}(H)$와 $T_{1}(D)$가 측정되었다. 실험데이타는 온도 30 K < T < 313 K 및 압력 0.67 atm < P < 1.92 atm에서 측정되어졌다. 핵자기공명 주파수는 각각 수소원자핵에 대해서 358.012 MHz였고 중수소원자핵에 대해서는 54.958 MHz였다. 측정된 $T_{1}(H)$와 $T_{1}(D)$의 비로부터 분자스핀 연산자들인 $J_{z}$와 ${3J_z}^{2}-J(J+1)$에 관련된 상관시간의 비 ${\tau}_{1}/{\tau}_{2}$의 값이 얻어졌다. J = 1 상태에서의 원자핵 스핀-격자 완화시간이 온도의 함수로서 $T^{0.25}$에 비례하는 것이 관측되었다.