• Journal of Magnetics
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• v.2 no.3
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• pp.101-104
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• 1997

Vector Moving Preisach model has been applied to the unoriented Co-based alloy thin film media. In the model, the out-of plane easy axis distribution of the particles was derived directly from the texture coefficient phkl obtained from XRD analysis, which corresponds to the fraction of the grains that have the {hkl} plane lying parallel to in-plane direction. The model was validated, by its prediction of a variety of responses, including major loop, minor loop, and the angular dependence of coercivities.

• Journal of the Korean Mathematical Society
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• v.56 no.6
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• pp.1515-1528
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• 2019

We show that large subsets of vector spaces over finite fields determine certain point configurations with prescribed distance structure. More specifically, we consider the complete graph with vertices as the points of $A{\subseteq}F^d_q$ and edges assigned the algebraic distance between pairs of vertices. We prove nontrivial results on locating specified subgraphs of maximum vertex degree at most t in dimensions $d{\geq}2t$.

• Bulletin of the Korean Mathematical Society
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• v.56 no.6
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• pp.1525-1537
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• 2019

Let ${\tilde{M}}$ be a Riemannian manifold equipped with a concircular vector field ${\tilde{X}}$ and M a submanifold (with its induced metric) of ${\tilde{M}}$. Denote by X the restriction of ${\tilde{X}}$ on M and by $X^T$ the tangential component of X, called the canonical field of M. In this article we study submanifolds of ${\tilde{M}}$ whose canonical field $X^T$ is also concircular. Several characterizations and classification results in this respect are obtained.

• Journal of Microbiology and Biotechnology
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• v.22 no.2
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• pp.190-198
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• 2012

RNA interference (RNAi) is rapidly becoming a valuable tool in biological studies, as it allows the selective and transient knockdown of protein expression. The short-interfering RNAs (siRNAs) transiently silence gene expression. By contrast, the expressed short-hairpin RNAs induce long-term, stable knockdown of their target gene. Trichoplusia ni (T. ni) cells are widely used for mammalian cell-derived glycoprotein expression using the baculovirus system. However, a suitable shRNA expression system has not been developed yet. We investigated the potency of shRNA-mediated gene expression inhibition using human and Drosophila U6 promoters in T. ni cells. Luciferase, EGFP, and ${\beta}$-N-acetylglucosaminidase (GlcNAcase) were employed as targets to investigate knockdown of specific genes in T. ni cells. Introduction of the shRNA expression vector under the control of human U6 or Drosophila U6 promoter into T. ni cells exhibited the reduced level of luciferase, EGFP, and ${\beta}$-N-acetylglucosaminidase compared with that of untransfected cells. The shRNA was expressed and processed to siRNA in our vector-transfected T. ni cells. GlcNAcase mRNA levels were down-regulated in T. ni cells transfected with shRNA vectors-targeted GlcNAcase as compared with the control vector-treated cells. It implied that our shRNA expression vectors using human and Drosophila U6 promoters were applied in T. ni cells for the specific gene knockdown.

• The Journal of Korean Society of Virology
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• v.26 no.2
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• pp.141-150
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• 1996

본 연구에서는 $CD4^+$ T 세포를 일회성으로 감염시킬 수 있고 $CD4^+$ T 세포에 HIV-1 envelope 유전자를 전달할 수 있는 바이러스 입자를 생산하는 HIV-1 complementation system을 개발하였고 이 system을 이용하여 $CD4^+$ T 세포에 선택적으로 HIV-1 envelope 당단백질을 발현시켰다. 이 system은 Gag/Gag-Pol expressor와 Env expressor로 구성되어있다. Gag/Gag-Pol expressor는 바이러스 입자 생산에 필요한 구조단백질과 기능단백질을 발현시키지만 packaging signal이 결핍되어 바이러스 입자로 유전자가 들어가지 못하도록 제조되었다. Env expressor는 Tat, Rev와 envelope 당단백질을 발현시키고 packaging signal을 갖고 있어 바이러스 입자로 envelope 유전자가 들어가도록 제조되었다. Gag/Gag-Pol expressor로부터 Gag와 Gag-Pol의 발현은 Rev 단백질을 요구하였고 Env expressor로부터 Rev 단백질 이 제공될 때 Gag와 Gag-Pol 단백질은 효율적으로 발현되었다. Gag/Gag-Pol과 Env expressor로 cotransfection된 COS-1 세포에서 $CD4^+$ T 세포를 일회성으로 감염시킬 수 있는 바이러스 입자가 생산되었다. 생산된 바이러스 입자는 $CD4^+$ T 세포에 HIV-1 envelope 유전자를 전달하여 envelope 당단백질을 발현시켰고 복제 가능한 자손 바이러스의 생성을 유도하지 못하였다. 본 연구에서 개발된 방법은 $CD4^+$ T 세포에서 envelope 당단백질의 기능을 분석하고 관심 있는 유전자를 $CD4^+$ T 세포에 전달하는 바이러스 입자의 생산에 이용할 수 있다.

• Bulletin of the Korean Mathematical Society
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• v.47 no.3
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• pp.551-561
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• 2010

In this paper we give a new characterization of real hypersurfaces of type B, that is, a tube over a totally geodesic $\mathbb{Q}P^n$ in complex two-plane Grassmannians $G_2(\mathbb{C}^{m+2})$, where m = 2n, with the Reeb vector $\xi$ belonging to the distribution $\mathfrak{D}$, where $\mathfrak{D}$ denotes a subdistribution in the tangent space $T_xM$ such that $T_xM$ = $\mathfrak{D}{\bigoplus}\mathfrak{D}^{\bot}$ for any point $x{\in}M$ and $\mathfrak{D}^{\bot}=Span{\xi_1,\;\xi_2,\;\xi_3}$.

• Journal of the Korean Institute of Telematics and Electronics B
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• v.33B no.2
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• pp.10-19
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• 1996

This paper presents a finger-shaped multisensor system which can measure the tyep and position of a target surface by contactl. The multi-sensor system consists of a sphere-shpaed optical tactile sensor located at the finger tip and a force/torque sensor located at the joint of a finger. The optial tactile sensor determines the type and position of the target surface using the shape and position of the CCD image of the touching area generated by a contact between the sensor and the taget surface. The force/torque sensor also determines the position and surface normal vector by applying the distributionof forces and torques t the contact point to the equations of finger shape. The measurements on the position and surface normal vector at a contact point obtined by two individual sensors are fused using a statistical method. The integrated sensor system has 0.8mm error in position measurement and 1.31$^{\circ}$ error in normal vector measurement. The developed sensor system has many applications, such as autonomous compliance control, automatic grasping and recognition, etc.

• Asian-Australasian Journal of Animal Sciences
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• v.33 no.6
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• pp.1023-1033
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• 2020

Objective: The efficiency of the knock-in process is very important to successful gene editing in domestic animals. Recently, it was reported that transient loosening of the nucleosomal folding of transcriptionally inactive chromatin might have the potential to enhance homologous recombination efficiency. The objective of this study was to determine whether histone deacetylases (HDAC) inhibitor and RAD51 recombinase (RAD51) expression were associated with increased knock-in efficiency on the β-casein (bCSN2) gene locus in mammary alveolar-large T antigen (MAC-T) cells using the transcription activator-like effector nucleases (TALEN) system. Methods: MAC-T cells were treated with HDAC inhibitors, valproic acid, trichostatin A, or sodium butyrate for 24 h, then transfected with a knock-in vector, RAD51 expression vector and TALEN to target the bCSN2 gene. After 3 days of transfection, the knock-in efficiency was confirmed by polymerase chain reaction and DNA sequencing of the target site. Results: The level of HDAC 2 protein in MAC-T cells was decreased by treatment with HDAC inhibitors. The knock-in efficiency in MAC-T cells treated with HDAC inhibitors was higher than in cells not treated with inhibitors. However, the length of the homologous arm of the knock-in vector made no difference in the knock-in efficiency. Furthermore, DNA sequencing confirmed that the precision of the knock-in was more efficient in MAC-T cells treated with sodium butyrate. Conclusion: These results indicate that chromatin modification by HDAC inhibition and RAD51 expression enhanced the homologous recombination efficiency on the bCSN2 gene locus in MAC-T cells.

• Journal of Veterinary Clinics
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• v.16 no.2
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• pp.328-331
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• 1999

Theileriosis에 대한 효율적인 예방대책을 마련하기 위한 일환으로 발현된 T. sergenti 재조합 항원단백질의 면역원성을 조사하였다. 먼저, E. coli 단백질 발현 vector인 pQE 32 plasmid vector를 이용하여 발현된 T. sergenti의 재조합 막표면단백질(KTs-MP)을 4개월령의 유우 송아지에 접종하였다. 그리고 접종된 송아지의 혈액변화상과 T. sergenti에 대한 항체가의 변화상을 분석한 결과, 재조합단백질의 접종에 의하여 항체가가 상승되는 것을 알 수 있었다. 그러나 재조합단백질의 접종만으로는 T. sergenti의 감염을 완전하게 예방하지는 못하였다.

• Journal of applied mathematics & informatics
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• v.7 no.2
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• pp.439-451
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• 2000

To compute derivatives using matrix vector multiplication method, new algorithms were introduced in [1.2]n By these algorithms, we reduced roundoff error in computing derivative using Chebyshev collocation methods (CCM). In this paper, some applications of these algorithms ar presented.