• Journal of the Society of Cosmetic Scientists of Korea
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• v.43 no.2
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• pp.93-102
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• 2017

In this study, various plant extracts were screened for the effective and safe skin care ingredient according to the antioxidant activity assay guidelines. We selected an optimized combination herbal extract, Charmzone extract (CZE), which composed of Nelumbo nucifera leaves, Saururus chinensis and Orostachys japonica. CZE exerted free radical scavenging activity. It reduced reactive oxygen species formation and increased total antioxidant capacities in human keratinocyte (HaCaT) and normal human dermal fibroblasts (NHDF). CZE also leaded procollagen type 1 secretion in NHDF and decreased cellular melanin contents in B16F10. The production of nitric oxide was decreased by CZE in lipopolysaccharide-stimulated RAW264.7 in a dose dependent manner. Therefore, it is concluded that CZE can be applied for naturally derived anti-aging functional skin care ingredient for anti-oxidation, wrinkle enhancement, whitening, anti-inflammation and wound healing of skin.

• Transactions of the Korean Society of Automotive Engineers
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• v.13 no.4
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• pp.135-144
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• 2005

The Effects of intake swirl and combustion parameters on the performance and emission characteristics in a V8 type turbocharged intercooler D.I. diesel engine of the displacement $16.7\iota$ were studied experimentally in this paper. Generally the swirl in the combustion process of diesel engine promotes mixing of the injection fuel and the intake air. Also, TCI diesel engine is put to practically use intercooler in order to increase boost efficiency which is cooled boost air. As a result of steady flow test, when the swirl ratio is increased, the mean flow coefficient is decreased, whereas the Gulf factor is increased. And through engine test, its can be effected to meet performance and emission by optimizing the main parameters; the swirl ratio is 2.25, compression ratio is 17.5, combustion bowl is re-entrant $8.5^{\circ}$, nozzle hole diameter is $\phi0.33^{\ast}3+\phi0.35^{\ast}2$, injection timing is BTDC $12^{\circ}CA$ and turbocharger is T02 model which are compressor 0.6A/R+46trim and turbine 1.0A/R+57trim.

• The Journal of the Petrological Society of Korea
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• v.23 no.2
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• pp.31-43
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• 2014

The Yeongdeok granite emplaced in the eastern Yeongyang subbasin is typically a medium- to coarse-grained massive biotite granite. It intruded into Precambrian schist & gneiss complex and is unconformably overlain by Cretaceous sedimentary rocks. In this study, we attempt to investigate the magma type which formed Yeongdeok granite and estimate the emplacement depth using Al-in-hornblende geobarometer to mineral composition. According to the magma fractionation, $TiO_2$, $Al_2O_3$, $Fe_2O_3{^*}$, FeO, $Fe_2O_3$, MnO, MgO, CaO, $Na_2O$ and $P_2O_5$ show positive trend but $K_2O$ indicate negative trend with $SiO_2$ contents. Those are identified as calc-alkaline series in AFM diagram and show the chemical characteristics of the I-type magma through the oxidation tendency of the iron ion and the portion of the alkaline composition. When calculated using the equation of Hollister et al. (1987), the emplacement depths of the Yeongdeok granite range from 8.98 to 17.19 km and average depth was estimated 13.03 km approximately.

• Proceedings of the Korean Society of Applied Pharmacology
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• 1994.04a
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• pp.186-186
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• 1994

Many agonists have been known to activate the hydrolysis of membrane phospholipids through the bindings with corresponding receptors on the various cells. Diacylglycerol and inositol 1,4,5-trisphosphate(IP3) generated by the action of phosphoinositide-specific phospholipase C (PI-PLC) are well known second messengers for the activation of protein kinase C and the mobilization of Ca2+ in many cells. Three types of PI-PLC isozyme (${\alpha}$,${\gamma}$, and $\delta$) and several subtrpes for each type have been identified from mammalian sources by purification of enzymes and cloning of their cDNAs. Each type PI-PLC isozyme is coupled to different receptors and mediators, for example, ${\beta}$-types are coupled to the seven-transmembrane-receptors via Gq family of G-proteins and ${\beta}$-types directly to the receptor tyrosine kinases. Specific modulators for the signaling pathway through each type of PI-PLC should be very useful as potential potential candidates for lend substances in developing novel drugs. To establish the sensitive and convenient screening systems for searching modulators on PI-PLC mediated signaling, two kinds of approaches have been tried. (1) Establishment of in vitro assay condition for each type of PI-PLC isozyme: Overexpression by using vaccinia virus and purification of each isozyme was carried out for the preparation of large amounts of enaymes. Optimum and sensitive assay condition for the measurements of PI-ELC activities were established. (2) Development of the cell lines in which each type of PI-PLC is permanently overexpressed: A fibroblast cell line (3T3${\gamma}$1-7) in which PI-PLC-${\gamma}$1 was overexpressed by using pZip-neo expression vector was developed and used for the measurement of PDGF-induced IP3 formation. The responses for IP3 formed in 3T3${\gamma}$1-7 cells by the treatment of PDGF is 8 times more sensitive than those in control cells. 3T3${\gamma}$l-7 cell is useful for the screening of the inhibitors on the PDGF-induced cellular responses from large number of samples in a small volume(50 ${\mu}$l) and short time(5-15 min). Using these systems, we screened hundreds of herb-extracts for the inhibition of PDGF-induced IP3 formation and selected several extracts that showed the inhibition as the candidates for isolation and characterization of active substances. The determination of the acting point of selected extracts or fractions in the PDGF signaling pathway has been analyzing.

• BMB Reports
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• v.51 no.1
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• pp.3-4
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• 2018

Parkinson's disease (PD) is a debilitating disorder resulting from loss of dopamine neurons. In dopamine deficient state, the basal ganglia increases inhibitory synaptic outputs to the thalamus. This increased inhibition by the basal ganglia output is known to reduce firing rate of thalamic neurons that relay motor signals to the motor cortex. This 'rate model' suggests that the reduced excitability of thalamic neurons is the key for inducing motor abnormalities in PD patients. We reveal that in response to inhibition, thalamic neurons generate rebound firing at the end of inhibition. This rebound firing increases motor cortical activity and induces muscular responses that triggers Parkinsonian motor dysfunction. Genetic and optogenetic intervention of the rebound firing prevent motor dysfunction in a mouse model of PD. Our results suggest that inhibitory synaptic mechanism mediates motor dysfunction by generating rebound excitability in the thalamocortical pathway.

• Journal of the Mineralogical Society of Korea
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• v.25 no.4
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• pp.221-231
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• 2012

Uljin cassiterite deposit had been known to be a pegmatitic origin derived from the Wangpiri (Buncheon) granitic gneiss of Precambrian period. Lithium ore also shows the same origin and its lithium bearing mineral was ascertained to be a taeniolite. But the presence of leucocratic granites which played the role of host rocks haven't been clearly designated yet in these provinces. Even though Bonghwa and Youngweol sericite deposits situated in the vicinities of Hambaeg syncline had been known to have their host rocks as Hongjesa Granites of Precambrian period and Pegmatitic migmatite of unknown age respectively. But younger leucocratic granites are characterized by more amounts of albite and sericite (muscovite-3T type) than those of the older granitic rocks which contain plenty of biotite and chlorites. Although the younger granites show rather higher contents of alkalies such as $Na_2O$ (0.13~8.03 wt%) and $K_2O$ (1.71~6.38 wt%), but CaO (0.05~1.21 wt%) is very deficient due to the albitization and greisenization. Manisan granite, which is assumed to be Daebo granite which intruded the Gyunggi Gneiss Complex was again intruded by leucocratic granite whose microclinized part changed into kaolins. Taebaegsan region shows a wide distribution of carbonate rocks which are especially favorable to the ore depositions. And the presence of alkali granites which formed in the later magmatic evolution are well known to be worthwhile to the prospections of various rare metals and REEs resources.

• Journal of Plant Biotechnology
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• v.29 no.2
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• pp.85-92
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• 2002

This study has been focused on improving transformation efficiency of rice using Agrobacterium tumefaciens. We have demonstrated the effect of this system when the GPAT gene related to the cold-resistance was transferred by Agrobacterium tumefaciens in rice. Transformation conditions were modified using intron $\beta$-glucuronidase (GUS) expression as a reporter gene in the rice. In this study, mature seed-derived calli of rice (Oruza sativa L. cv. Dongjin) were pre-cultured for 3 days and then infected with Agrobacterium. When this infected calli were cultured in the dark for 10 days on co-cu]lure medium containing 50 mg/L of CaCl$_2$, 30 mg/L of acetosyringone, 2 mg/L of 2,4-D, 120 mg/L of betaine, high GUS expression was observed. In the present transformation system, the efficiency of transformation of GPAT gene was about 54%. Stable integration of GPAT gene into chromosomal DNA was proven by southern blot analysis of genomic DNA isolated from T$_{0}$ progenies. The progenies (T1 generation) derived from primary transformant of 5 lines were segregated with a 3 (resistant) : 1 (sensitive ratio) in medium containing hygromycin. This high frequency transformation system can be used as a useful tool in transformation of another monocotyledon.n.

• Korean Journal of Poultry Science
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• v.31 no.3
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• pp.177-185
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• 2004

This experiment was conducted to examine the dietary effects of local granular ark shell(GAS) replacing limestone on egg shell quality in aged hens. A total of 200 Hy-Line Brown layers of 54-weeks-old were allotted into four treatments with five replications each. They were fed control(limestone only) 1% GAS, 2% GAS and 3% GAS diets for 6 weeks. GAS was substituted for coarse-type limestone at 0, 1, 2 or 3% of diet on weight basis. Particle sizes of limestone and GAS were within the ranges of 2~4 mm in diameters. In vitro HCl solubility of GAS was significantly lower(6.27 vs. 7.90%) compared to that of limestone(p < 0.05). The dietary treatment did not affect feed intake and egg production rate of the birds. Egg shell strength and thickness tended to be improved for birds fed the diets containing higher levels of GAS. The average egg shell strength and thickness of 2 or 3% GAS groups were significantly greater than those of the control (P < 0.05). The Tibial ash content of birds from 3% GAS group increased significantly as compared to that of the control. Tibial breaking strength, however, was not affected by the dietary treatment. Serum Ca and P contents of the birds were also not different among the dietary treatments. In conclusion, locally prepared GAS can be used as a calcium source in aged layer diet.

• Journal of the Society of Cosmetic Scientists of Korea
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• v.44 no.2
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• pp.191-200
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• 2018

In this study, we conducted research to find anti-wrinkle skin care ingredients from natural sources via the measurement of procollagen type 1 levels in the medium of normal human dermal fibroblast (NHDF) and found that a Nelumbo nucifera leave extract (NLE) was as the best effective ingredient. By the high performance liquid chromatography analysis, we confirmed isoquercitrin was one of the main compounds of NLE. Moreover, it also increased the procollagen type 1 production without cytotoxicity of NHDF. NLE and isoquercitrin exerted free radical scavenging activity. Especially, isoquercitrin exhibited strong intracellular antioxidant capacity in human skin-derived cells, HaCaT and NHDF. Finally, we performed clinical test for the inhibitory effect of NLE on skin wrinkle formation. A randomized study was conducted in 22 healthy female volunteers, aged between 30 and 65 yrs, with moderate to moderately severe facial wrinkles. Volunteer applied a 1.7% NLE cream (isoquercitrin 51 ppm) and a control cream at each facial side (left/right) twice daily for 8 weeks. The 1.7% NLE cream improved skin roughness through reducing the wrinkle of the craw's feet significantly without any skin side effect. Our results demonstrate that NLE and isoquercitrin can increase the collagen production and exert antioxidant activity. Therefore, we expect that the new non-toxic herbal extract, isoquercitrin-containing NLE will be interesting natural ingredient of cosmetics with anti-wrinkle efficacy.

• Journal of Physiology & Pathology in Korean Medicine
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• v.17 no.5
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• pp.1281-1287
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• 2003

In this research 2 sample compounds are made and analyzed in terms of the in vivo and in vitro effects on the ovariectomized rats. The 2 compounds are prescribed based on pathologic patterns of osteoporosis, and added calcium citrate from tuna bone powder. SG is for menopausal women(Type I osteoporosis) and GN is for senile men(Type II osteoporosis). Through these, SG manifested Significant effects on the T4, osteocalcin level, and through the histological changes of osteoid tissues and lipocytes. On the other hand GN showed significant increase on the biochemical markers of osteocalcin, TALP, even in histological features and bone mineral density and intensity of femur it showed meaningful changes. But In the results of RT-PCR on the IL-1β, IL-6, TNF α, there weren't coherent results with in vivo test, that is they were increased in the sample compound group than control group. These increase of bone resorption was seemed that those cytokines had the osteoclasts promote their own resorptive functions after fragments of bone tissues were increased in the cavity. And this clearance of inner fragments help the bone to strengthen its own substance. Putting together above facts, the sample compounds, SG and GN, made of tuna bone powder and herbal solutions are predicted that there would be pharmacological actions improving the osteoporosis initiated from the disorders of calcic absorption and increase of bone resorption. And GN has more effective actions than SG at least in the animal model.