• Microbiology and Biotechnology Letters
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• v.31 no.2
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• pp.103-110
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• 2003

The regulation of pyrimidine nucleotide synthesis has been proved to be controlled by a regulatory protein PyrR-mediated attenuation in the Gram-positive bacteria. After several bacterial genome sequencing projects, we have discovered the PyrR orthologues in the databases for Haemophilus influenzae and Synechocystis and sp. PCC6803 genome sequences. To investigate whether these PyrR orthologue proteins regulate pyrimidine nucleotide synthesis as well as the cases of Bacillus, the PyrR regions of each strains were amplified by PCR and cloned with pUC19 or T-vector in Escherichia coli and with a shuttle vector pHPS9 for E. coli and B. subtilis. For the regulation test of the PyrR orthologues, the aspartate-transcarbamylase (ATCase) assay was carried out. From the results of the ATCase assay, it was confirmed that Synechocystis sp. PCC6803 could not restore by pyrimidines to a B. subtilis, PyrR but H. influenzae PyrR could. For Purification of PyrR orthologue proteins, PyrR orthologue genes were cloned into the expression vector (pET14b). Over-expressed product of PyrR orthologue genes was purified and analyzed by the SDS-PACE. The purified PyrR orthologue proteins from H. influenzae and Synechocystis sp. PCC6803 turned out to be molecular mass of 18 kDa and 21 kDa, respectively. The result of uracil phosphoribosyl transferase (UPRTase) assay with purified PyrR orthologue proteins showed that H. influenzae PyrR protein only has UPRTase activity. In addition, we could predict several regulatory mechanisms that PyrR orthologue proteins regulate pyrimidine de novo synthesis in bacteria, through phylogenetic analysis for PyrR orthologue protein sequences.

• Korean Journal of Social Welfare
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• v.60 no.3
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• pp.253-274
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• 2008

The link between work life and family life is an essential subject matter in understanding the lives of dual-earner couples. Concepts of spillover and crossover explain the link between work and family. The present study examines both the positive and negative aspects in these processes. The data come from the Sloan Working Families Study conducted by the Alfred P. Sloan Center on Parents, Children, and Work and NORC at the University of Chicago. The Experience Sampling Method employed explores directly the daily life experiences of the participants. The data were analysed using t-test. Both spillover and crossover were found in the lives of dual-earner couples in this study. Men and women brought happy emotions at work to home, but the data provide limited support for spillover of negative emotions. Gender differences were more apparent in examining the process of crossover. Men appear responsive to the positive and negative emotional experiences their spouse brought home while women were found not to be responsive to their spouses' positive emotional experiences at work. Furthermore, the analysis revealed an interesting trend concerning the emotions of working couples in that they generally seem to recover to their average level of emotions once home. This suggests that home can be a respite from strong emotions, a comforting place. By looking closely into the emotions experienced by working parents in their daily lives, this study adds contextual understanding concerning the link between work and family life. The findings on the effects of positive experiences at work invite social work practitioners and researchers to further investigate the phenomena of spillover and crossover processed in greater detail, taking into account this contextual aspect of family life as well as the work life of dual-earner couples.

• Journal of Bio-Environment Control
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• v.29 no.3
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• pp.259-264
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• 2020

In this study, the humidity control effect of a counter-flow ventilator was analyzed in a greenhouse with high relative humidity at night in the winter season. A case of the counter-flow ventilator was 0.96 × 0.65× 0.82(W × D × H, m) and there were heat transfer element and two fans for air supply and exhaust in the counter-flow ventilator. Two counter-flow ventilators were used in this study and the setting humidity of the ventilators was 80%. The temperature and relative humidity at night(18:00-8:00) in the greenhouse were measured. In a greenhouse without a counter-flow ventilator, the average temperature and humidity was 14.9℃, 82.8%, respectively. When the counter-flow ventilator was operated, the corresponding averages were 15.1℃, 79.9%. The independent sample t test of monthly temperature and relative humidity showed no difference in temperature, and a significant difference in relative humidity with 1% of the significance level. Therefore, using the counter-flow ventilator helps to control relative humidity in greenhouse and increase yield.. And further research considering the pros and cons of using the counter-flow ventilator is needed.

• Journal of the Korean Society of Marine Environment & Safety
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• v.21 no.6
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• pp.696-703
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• 2015

This paper is a follow-up research of 'A study on the development of Safety Management Evaluation Index for Shipping Company'. It aims to develop the evaluation items of Safety Management Index(hereafter called as 'SMI') applicable to 'Safety Advantage Point', which is suggested as the sub-index of 'Safety Management Evaluation Index' function in the preceding research and also verify their adequacy. For that purpose, firstly, the 11 evaluation items were developed after collecting the practical opinions from safety management personnels in Korean shipping companies. Secondly, the survey was carried out for the verification of the adequacy of the 11 evaluation items. And lastly, its results was analysed, whether it has statistical meanings of differences in adequacy awareness amongst safety managers grouped depending on business type(coastal, ocean-going) and business capacity(number of owned-operating ships). As a result, there was no relevance between their adequacy awareness and business type of their companies. However, a difference in their adequacy awareness existed depending on their companies' business capacity(small, medium and large sized). In conclusion, when the safety management level of shipping companies is evaluated by SMI, the same indices can be applicable to both coastal and ocean-going shipping companies by a grouping of companies' business capacity.

• Journal of Industrial Convergence
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• v.18 no.1
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• pp.45-52
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• 2020

This study is a descriptive study conducted to investigate the effect of smoking cigarette sign sold in Korea on the smoking cessation effects and to use it as a basis for future smoking cessation and health related health data. To this end, we surveyed 500 men and women over 20 years of age on the Internet Naver web using Naver form questionnaire for 10 days from October 1, 2018 using blogs and cafes. The analyzed data were analyzed by factor analysis, T-test and correlation using SPSS WIN20.0. The main findings were as follows: First, the cigarette warning picture harmful to human body had the biggest influence on the smoking cessation effect. Second, the smoking cessation effect had the highest correlation with the pre-question consciousness about the cigarette warning picture. This indicates that the cigarette pack warning is considered the most sensitive part of smokers. Based on the results of this paper, diversification of cigarette warning picture and regular replacement of warning picture are expected to provide useful basic data for suggesting practical measures to prevent smoking. do. Therefore, the smoking warning picture is widely promoted through the media and the media, which has a positive effect on the practice of quitting smoking to the public, and is also used as a basic data for the health policy for the health promotion of the public.

• Journal of Korean Society of Disaster and Security
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• v.13 no.3
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• pp.29-43
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• 2020

The purpose of this study was to identify the priority of the ISO auditors' specialized knowledge and job skills required to perform their duties, along with their personal characteristics, in order to enhance field-based auditing capability. For achieving the objectives of the study, the questionnaire consisted of a total of 50 competency factors, adding personal characteristics area to the ISO 19011-based specialized knowledge and job skills areas and the survey was conducted on the currently active ISO auditors with a five-point recert scale. Based on the results of the survey, the importance and present of ISO auditors in 3 competency areas were verified statistically significant difference through a t-test. The top priority and next priority competency factors for the overall competency and each competency areas were obtained through the Borich coefficient and the Locus for Focus Model. Considering the top priority competency factors in each competency areas identified from the study results, it can help develop related auditing curriculum. for improving the auditors' competency, and furthermore, it is expected to help strengthen ISO management system including quality, health & safety, environment and business continuity for companies.

• Journal of Gifted/Talented Education
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• v.26 no.1
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• pp.161-185
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• 2016

The purpose of this study was to investigate for gifted elementary students in science the feature of character, life goals and happiness, and the effect relationship on happiness based on the relationship between factors. For this, independent t-test, multi-linear regression analysis and hierarchical regression were conducted. The results from this study are as follows. First, scientifically gifted elementary students show higher level of responsibility, ethics, positive self-understanding and contribution goal, but lower in material and image goal than general students. Second, character, life goal and happiness are correlated. Third, female students rather than male students and students with consideration/service character have intrinsic goals. While, the students with higher level of consideration/service and lower level of sympathy show extrinsic goals. The higher the level of consideration/service and the lower of self-control they have the higher their happiness are. Fourth, as scientifically gifted elementary students have more consideration and relationship goal, their happiness go up. While, the more they have self-growth and material goal, the lower the happiness. Fifth, the character of scientifically gifted elementary students is the factor that explains the effect on happiness more easily than life goal, relatively. The factor of life goal mediates the consideration/service and happiness. In conclusion, I hope that this study contribute to raise the happiness of scientifically gifted elementary students, and considerate the character education and counseling program for character development.

• Journal of Pharmaceutical Investigation
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• v.41 no.3
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• pp.191-196
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• 2011

In this study simple and sensitive high performance liquid chromatographic method using a commercially available column, was developed and validated for the determination of zolpidem tartrate in human plasma. The developed method with suitable validation was applied to a bioequivalence study of two different kinds of zolpidem tartrate. Two different formulations containing 10 mg of zolpidem tartate (CAS : 99294-93-6) were compared in 24 healthy male volunteers in order to compare the bioavailability and prove the bioequivalence. The study was performed in an open, single dose randomized, 2-sequence, cross-over design in 24 healthy male volunteers with a one-week washout period. Blood samples for pharmacokinetic profiling were drawn at selected times during 12 h. The mean $AUC_{0-12h}$, $C_{max}$, $T_{max}$ and $T_{1/2}$ were $676.6{\pm}223.4$ $ng{\cdot}h{\cdot}mL^{-1}$, $177.4{\pm}34.2$ $ng{\cdot}mL^{-1}$, and $0.8{\pm}0.4$ and $3.5{\pm}2.1$, respectively, for the test formulations, and $640.7{\pm}186.6$ $ng{\cdot}h{\cdot}mL^{-1}$, $193.0{\pm}64.5$ $ng{\cdot}mL^{-1}$, and $0.9{\pm}0.4$ and $2.7{\pm}0.9$, respectively, for the reference formulation. Both primary target parameters $AUC_{0-12h}$ and $C_{max}$ were log-transformed and tested parametrically by analysis of variance (ANOVA). 90% confidence intervals of $AUC_{0-12h}$ and $C_{max}$ were in the range of acceptable limits of bioequivalence (80-125%). Based on these results, the two formulations of zolpidem tartate are considered to be bioequivalent.

• Restorative Dentistry and Endodontics
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• v.30 no.5
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• pp.393-401
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• 2005

The purpose of this study was to compare the canal configuration after shaping by ProTaper rotary files and ProTaper hand files in resin simulated canals. Forty resin simulated canals with a curvature of J-shape and S-shape were divided into four groups by 10 blocks each Simulated root canals in resin block were prepared by ProTaper rotary files and ProTaper hand files using a crown-down pressureless technique All simulated canals were prepared up to size $\#25$ file at end-point of preparation. Pre- and post-instrumentation images were recorded with color scanner. Assessment of canal shape was completed with an image analysis program. Measurements were made at 0, 1, 2, 3, 4, 5, 6 and 7 mm from the apex. At each level, outer canal width, inner canal width, total canal width, and amount of transportation from original axis were recorded. Instrumentation time was recorded. The data were analyzed statistically using independent t-test. The result was that ProTaper hand files cause significantly less canal transportation from original axis of canal body and maintain original canal configuration better than ProTaper rotary files, however ProTaper hand files take more shaping time.

• Korean Journal of Plant Taxonomy
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• v.46 no.1
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• pp.1-12
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• 2016

DNA sequences of nrITS regions obtained from 49 accessions representing 12 species and one variety within the section Adonanthe of the genus Adonis were analyzed to test the previous intra-sectional classification system and to determine their phylogenetic relationships. The results showed that the seven accessions of A. amurensis Regel et Radde included in the present study did not form a monophyletic group, as some of the accessions showed a close relationship with the Japanese endemic species A. ramosa Franch., which implies that the current species delimitation and identification of A. amurensis is problematic. Adonis pseudoamurensis W. T. Wang, which is frequently misidentified as A. ramosa in Korea, formed a segregated group, which suggests that they should not be considered as conspecific taxa. Accessions from A. shikokuensis Nishikawa et Koji Ito, A. multiflora Nishikawa et Koji Ito, and A. pseudoamurensis formed a clade, but monophyly of each species was not evident. The nrITS data did not support the classification system proposed by Wang, who classified sect. Adonanthe into four series, as most of these were found to be either polyphyletic or paraphyletic.