• BMB Reports
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• v.43 no.11
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• pp.732-737
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• 2010

RNA interference is a post-transcriptional silencing mechanism triggered by the bioavailability and/or exogenous introduction of double-stranded RNA (dsRNA) into cells. Here we describe a novel method for the synthesis of siRNA in a single vessel. The method employs in vitro transcription and a single-stranded DNA (ssDNA) template and design, which incorporates upon self-annealing, two promoters, two templates, and three loop regions. Using this method of synthesis we generated efficacious siRNAs designed to silence both exogenous and endogenous genes in mammalian cells. Due to its unique design the single-stranded template is easily amenable to adaptation for attachment to surface platforms for synthesis of siRNAs. A siRNA synthesis platform was generated using a 3' end-biotinylated ssDNA template tethered to a streptavidin coated surface that generates stable siRNAs under multiple cycles of production. Together these data demonstrate a unique and robust method for scalable siRNA synthesis with potential application in RNAi-based array systems.

• Journal of the Korea Institute of Information and Communication Engineering
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• v.16 no.3
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• pp.571-578
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• 2012

In this paper, we propose two main points. The first is the constraint for logic synthesis, and the second is an efficient logic synthesis method. Logic synthesis is a process to obtain the gate-level netlist from RTL (register transfer level) codes using logic mapping and optimization with the specified constraints. The result of logic synthesis is tightly dependent on constraint and logic synthesis method. Since the size and timing can be dramatically changed by these, we should precisely consider them. In this paper, we present the considering items in the process of logic synthesis by using our experience and experimental results. The proposed techniques was applied to a circuit with the hardware resource of about 650K gates. The synthesis time for the hybrid method was reduced by 47% comparing the bottom-up method and It has better timing property about slack than top-down method.

• Proceedings of the Korean Society of Precision Engineering Conference
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• 2004.10a
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• pp.1270-1273
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• 2004

The mechanism design is synthesis of suitable mechanism which can be output motions about input motions. That has generally two steps which are the type synthesis and the dimensional synthesis. And required mechanism analysis step for confirming middle or final result. The type synthesis is definition of mechanism type which required aim and the dimensional synthesis is calculation of dimension about defined type mechanism. The type synthesis of mechanism is included qualitative design field which isn't defined systematic design method. especially, the most difficult step for mechanism design automation. In this paper proposed the component modular design method which is figured mechanism types automate with component modules using component modular approach. And develop CAD(Computer Aided Program) program for application.

• Bulletin of the Korean Chemical Society
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• v.34 no.4
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• pp.1070-1076
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• 2013

Herein is reported a simple and efficient synthesis of isoflavones starting from various substituted phenacyl bromides and salicylaldehydes in presence of NHC. The mechanism involved domino catalysis type approach with consumption and regeneration of catalyst in two catalytic cycles. This method proved to be very lucrative and gives very good yield. The method described here represents an environmentally benign alternative to classical approach.

• Proceedings of the KSME Conference
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• 2008.11a
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• pp.822-826
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• 2008

A linkage mechanism is a device to convert an input motion into a desired output motion. Traditional linkage mechanism designs are based on trial and error approaches so that size or shape changes of an original mechanism often result in improper results. In order to resolve these problems, an improved automatic mechanism synthesis method that determines the linkage type and dimensions by using an optimization method during the synthesis process has been proposed. For the synthesis, a planar linkage is modeled as a set of rigid blocks connected by zero-length translational springs with variable stiffness. In this study, the sizes of rigid blocks were also treated as design variables for more general linkage synthesis. The values of spring stiffness and the size of rigid block yielding a desired output motion at the end-effecter are found by using an optimization method.

• Proceedings of the Acoustical Society of Korea Conference
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• 1991.06a
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• pp.45-49
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• 1991

In area of the speech synthesis, the waveform coding with high quality are mainly used to the synthesis by analysis. However, it is difficult to applying the waveform coding to the synthesis by rule, because the parameters of this coding are not classified as either excitation parameters and vocal tract parameters. In this paper, we proposed a new pitch change method that can alter the pitch periods in the waveform coding. The proposed method expands the pitch period by the LPC synthesis method, and then the period is compressed by the waveform halving technique. Thus, it is possible that the waveform coding is carried out the synthesis by rule in speech processing.

• Bulletin of the Korean Chemical Society
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• v.28 no.9
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• pp.1493-1498
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• 2007

In this work, alkyl methacrylate-based microgels were synthesized by an experimental design method, and their sebum absorption characteristics were investigated. The results of fractional factorial experimentation indicated that the cross-linking agent content, solvent content, and stirring speed were the main parameters in the synthesis of the microgels. The suitable synthesis conditions were determined by the response surface design method. Through a study of the monomer and solvent effects, it was confirmed that the microgel shows the highest sebum absorption ratio when t-butyl methacrylate is used as a monomer or when acetone is used as a solvent. The optimal microgel synthesis conditions for cosmetic application were determined, and the resulting microgel had a mean particle size of 4.7 μm and a sebum absorption ratio of 435%.

• Transactions of the Korean Society of Mechanical Engineers A
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• v.22 no.2
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• pp.399-407
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• 1998

This paper deals with a method for finding the physical structure of dynamic systems which shows reasonable response to a given specifiation. The method uses only four basic models of bond graph prototypes which have been originally proposed by the authors as a general model for dynamic systems, and then makes its procedure highly physical in the sense that it can synthesize a dynamic system through the structural transformation directly on bond graph models without any mathematical manipulation. Also, it is shown that this method has further advantages in optimizing parameters for an existing system rather than developing design concepts for a new device, the latter being more suitable using the so-called analytical synthesis method introduced by Park and Redfield. One example serves to trace the outlines of the direct synthesis method proposed in this paper for dynamic systems in terms of bond graph prototypes.

• Proceedings of the Korean Society for Noise and Vibration Engineering Conference
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• 2000.11a
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• pp.201-206
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• 2000

In this work transfer function synthesis method based on FRF data of each substructure is investigated for a complex structure composed of many substructures. Though the transfer function synthesis method has superiority to analyze the characteristics of interfaces among substructures effectively, many problems arise in the computation process, especially matrix inversion process. Due to computational problems, the error between the data obtained by test and the predictions through computations is inevitable. So in this paper, computational aspects in the transfer function synthesis method are examined through a steering system problem of passenger car. For the FBS method, frequency response functions of 3 substructures are measured experimentally. Effects of several parameters such as matrix inversion method, connection conditions between substructures and off-diagonal terms on system response are studied numerically.

• BMB Reports
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• v.38 no.5
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• pp.517-525
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• 2005

Solid phase peptide synthesis method, which was introduced by Merrifield in 1963, has spawned the concept of combinatorial chemistry. In this review, we summarize the present technologies of solid phase peptide synthesis (SPPS) that are related to combinatorial chemistry. The conventional methods of peptide library synthesis on polymer support are parallel synthesis, split and mix synthesis and reagent mixture synthesis. Combining surface chemistry with the recent technology of microelectronic semiconductor fabrication system, the peptide microarray synthesis methods on a planar solid support are developed, which leads to spatially addressable peptide library. There are two kinds of peptide microarray synthesis methodologies: pre-synthesized peptide immobilization onto a glass or membrane substrate and in situ peptide synthesis by a photolithography or the SPOT method. This review also discusses the application of peptide libraries for high-throughput bioassays, for example, peptide ligand screening for antibody or cell signaling, enzyme substrate and inhibitor screening as well as other applications.