Recyclable single-stranded DNA template for synthesis of siRNAs
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Ali, Mussa M.
(Department of Pharmacology, Tulane University Health Sciences Center)
Obregon, Demian (Humesis Biotechnology Corporation) Agrawal, Krishna C. (Department of Pharmacology, Tulane University Health Sciences Center) Mansour, Mahmoud (Department of Anatomy, Physiology and Pharmacology, College of Veterinary Medicine, Auburn University) Abdel-Mageed, Asim B. (Department of Urology, Tulane University Health Sciences Center) |
1 | Oleinikov, A. V., Zhao, J. and Gray, M. D. (2005) RNA interference by mixtures of siRNAs prepared using custom oligonucleotide arrays. Nucleic. Acids. Res. 33, e92. DOI ScienceOn |
2 | Yu, J. Y., DeRuiter, S. L. and Turner, D. L. (2002) RNA interference by expression of short-interfering RNAs and hairpin RNAs in mammalian cells. Proc. Natl. Acad. Sci. U.S.A 99, 6047-6052. DOI ScienceOn |
3 | Luo, B., Heard, A. D. and Lodish, H. F. (2004) Small interfering RNA production by enzymatic engineering of DNA (SPEED). Proc. Natl. Acad. Sci. U.S.A. 101, 5494-5499. DOI ScienceOn |
4 | Davis, R., Jia, D., Cinar, B., Sikka, S. C., Moparty, K., Zhau, H. E., Chung, L. W., Agrawal, K. C. and Abdel-Mageed, A. B. (2003) Functional androgen receptor confers sensitization of androgen independent prostate cancer cells to anticancer therapy via caspase activation. Biochem. Biophy. Res. Commun. 309, 937-945. DOI ScienceOn |
5 | Caplen, N. J., Parrish, S., Imani, F., Fire, A. and Morgan, R. A. (2001) Specific inhibition of gene expression by small double-stranded RNAs in invertebrate and vertebrate systems. Proc. Natl. Acad. Sci. U.S.A. 98, 9742-9747. DOI ScienceOn |
6 | Abdel-Mageed, A. B. and Agrawal, K. C. (1998) Activation of nuclear factor Kappa B: potential role in metallothionein-mediated mitogenic response. Cancer Res. 58, 2335-2338. |
7 | Honore, B., Vorum, H. and Baandrup, U. (1999) hnRNPs H, H' and F behave differently with respect to posttranslational cleavage and subcellular localization. FEBS Lett. 456, 274-280. DOI ScienceOn |
8 | Abdel-Mageed, A. B. and Agrawal, K. C. (1997) Antisense down-regulation of metallothionein induces growth arrest and apoptosis in human breast carcinoma cells. Cancer Gene Ther. 4, 199-207. |
9 | Jiang, M., Arzumanov, A. A., Gait, M. J., Milner, J. A. (2005) Bi-functional siRNA construct induces RNA interference and also primes PCR amplification for its own quantification. Nucleic. Acids. Res. 33, e151. DOI ScienceOn |
10 | Rose, S. D., Kim, D. H., Amarzguioui, M., Heidel, J. D., Collingwood, M. A., Davis, M. E., Rossi, J. J. and Behlke, M. A. (2005) Functional polarity is introduced by dicer processing of short substrate RNAs. Nucleic. Acids. Res. 33, 4140-4156. DOI ScienceOn |
11 | Castanotto, D. and Rossi, J. J. (2004) Construction and transfection of PCR products expressing siRNAs or shRNAs in mammalian cells. Methods Mol. Biol. 252, 509-514. |
12 | Kim, J., Kim, H., Lee, Y., Yang, K., Byun, S. and Han, K. A. (2006) Simple and economical short-oligonucleotidebased approach to shRNA generation. J. Biochem. Mol. Biol. 39, 329-334. DOI |
13 | MacDonald, L. E., Zhou, Y. and McAllister, W. T. (1993) Termination and slippage by bacteriophage T7 RNA polymerase. J. Mol. Biol. 232, 1030-1047. DOI ScienceOn |
14 | Tomari, Y. and Zamore, P. D. (2005) Perspective: machines for RNAi. Genes Dev. 15, R61-R64. |
15 | Donze, O. and Picard, D. (2002) RNA interference in mammalian cells using siRNAs synthesized with T7 RNA polymerase. Nucleic. Acids. Res. 30, e46. DOI ScienceOn |
16 | Strat, A., Gao, L., Utsuki, T., Cheng, B., Nuthalapaty, S. Mathis, J. M., Odaka, Y. and Giordano, T. (2006) Specific and nontoxic silencing in mammalian cells with expressed long dsRNAs. Nucleic. Acids. Res. 34, 3803-3810. DOI ScienceOn |
17 | Milligan, J. F., Groebe, D. R., Witherell, G. W. and Uhlenbeck, O. C. (1987) Oligoribonucleotide synthesis using T7 RNA polymerase and synthetic DNA templates. Nucleic. Acids. Res. 15, 8783-8798. DOI |
18 | Elbashir, S. M., Harborth, J., Lendeckel, W., Yalcin, A., Weber, K. and Tuschl, T. (2001) Duplexes of 21-nucleotide RNAs mediate RNA interference in cultured mammalian cells. Nature 441, 494-498. DOI ScienceOn |
19 | Sohail, M., Doran, G., Riedemann, J., Macaulay, V. and Southern, E. M. (2003) A simple and cost-effective method for producing small interfering RNAs with high efficacy. Nucleic. Acids. Res. 31, e38. DOI ScienceOn |
20 | Reynolds, A., Leake, D., Boese, Q., Scaringe, S., Marshall, W. S. and Khvorova, A. (2004) Rational siRNA design for RNA interference. Nature Biotechnol. 3, 326-330. |
21 | Donze, O. and Picard, D. (2002) RNA interference in mammalian cells using siRNAs synthesized with T7 RNA polymerase. Nucleic. Acids. Res. 30, e46. DOI ScienceOn |
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