• YAKHAK HOEJI
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• v.35 no.4
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• pp.332-334
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• 1991

The synthesis of melandrin has been accomplished in good yield by the reaction of 5-tosyloxyanthranilic acid ethyl ester with p-acetoxybenzoyl chloride, followed by removal of protecting groups with methanolic ammonia.

• BMB Reports
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• v.32 no.2
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• pp.196-202
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• 1999

The existence of the Ras-independent signal transduction pathway of insulin leading to DNA synthesis was investigated in Rat-1 fibroblasts overexpressing human insulin receptor (HIRc-B) using the single-cell microinjection technique. Microinjection of a dominant-negative mutant $Ras^{N17}$ protein into quiescent HIRc-B cells inhibited the DNA synthesis stimulated by insulin. Microinjection of oncogenic H-$Ras^{V12}$ protein ($H-Ras^{V12}$) (0.1 mg/ml) induced DNA synthesis by 35%, whereas that of control-injected IgG was induced by 20%. When the marginal amount of oncogenic H-$Ras^{V12}$ protein was coinjected with a dominant-negative mutant of the H-Ras protein ($Ras^{N17}$), DNA synthesis was 35% and 74% in the absence and presence of insulin, respectively. This full recovery of DNA synthesis by insulin suggests the existence of the Ras-independent pathway. The same recovery was observed in the cells coinjected with either H-$Ras^{V12}$ plus H-$Ras^{N17}$ plus SH2 domain of the p85 subunit of PI3-kinase ($p85^{SH2-N}$) or H-$Ras^{V12}$ plus H-$Ras^{N17}$ plus interfering anti-Shc antibody. When co-injected with a dominant-negative H-$Ras^{N17}$, the DNA synthesis induced by the Ras-independent pathway was blocked. These results indicate that the Ras-independent pathway of insulin leading to DNA synthesis exists, bypassing the p85 of PI3-kinase and Shc protein, and requires Rac1 protein.

• Asian-Australasian Journal of Animal Sciences
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• v.14 no.1
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• pp.17-20
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• 2001

The effect of chicken IGF-I on protein synthesis of chicken embryo myoblasts cultured in serum-free medium was examined. When myoblasts were expanded to approximate 20-30% of well, the medium was changed to the serum-free medium including 0, 2, 20, 200 or 2000 ng/ml of recombinant chicken IGF-I. The culture medium including 10% fetal calf serum (FCS) was used as positive control. After 1 day of incubation, protein synthesis was measured by the incorporation of [$^3H$]-L-leucine. Thereafter cells were continued to incubate for further 18 hours, and the radioactivity in the protein was measured as an index of protein synthesis. The values for protein synthesis cultured in the serum-free medium without chicken IGF-I or with 2000 ng/ml of chicken IGF-I were the lowest. Protein synthesis was elevated with increasing chicken IGF-I concentration from 0 to 20 ng/ml. The values for protein synthesis in the 20 ng/ml and 200 ng/ml IGF-I groups were about half of that of the FCS group. The present study revealed that the potency of chicken IGF-I at the levels of 20 to 200 ng/ml to stimulate myoblast protein synthesis was about half of that of 10% FCS.

• Journal of the Korean Institute of Telematics and Electronics B
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• v.31B no.1
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• pp.57-63
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• 1994

The logic function synthesis using ULM U$_h$ is constructed based on canonic Reed-Muller expansion coefficient for a given function. This paper proposes the fast synthesis algorithm using ULM U$_h$ for ternary function. By using circuit cost and synthesis method of proposed in this paper, order of control input variable minimum number of ULM U$_h$ can be decided in the synthesis of n-variable ternary function. Accordingly, this method enables to optimum circuit realization for ternary function synthesis using ULM ULM U$_h$ and can be applied to ternary function synthesis using ULM U$_h$. The complexity of search for select the order of all control input variables is (n+2)(n-1)/2.

• Journal of the Korea Computer Graphics Society
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• v.15 no.1
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• pp.17-23
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• 2009

We present a simple new idea to improve the quality of exemplar based texture synthesis using multiple rotated input exemplars. Our algorithm successfully obtain rotational synthesis feature variations and manages to reduce the artifacts in the results, especially patch seams due to the structure of the exemplars provided which have been inappropriate for previous neighborhood matching synthesis algorithms. Our algorithm is parallel in nature, thus it is possible to implement our algorithm using GPU or multi-core CPU to accelerate synthesis process.

• Journal of Advanced Marine Engineering and Technology
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• v.24 no.1
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• pp.82-87
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• 2000

The component mode synthesis method(CMS) used for vibration analysis has demerit that error becomes larger, as degree of natural frequency grows higher. The reason of error occurrence is that Guyan's static reduction is used and the number of modes taken in each component is deficient. This paper proposes the substructure synthesis method using dynamic reduction to solve the problem from the component mode synthesis method. Computer simulation for the proposed method. FEM and the component mode synthesis method(CMS) on a rectrangular plate has been carried out to prove the avilability of the proposed method.

• Transactions of the Korean Society of Mechanical Engineers A
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• v.29 no.5 s.236
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• pp.716-725
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• 2005

The method of substructure synthesis o. component mode synthesis(CMS) provides an effective means of dynamic analysis of very large and/or complex structures. In this study, residual dynamic flexibility attachment modes in hybrid component mode synthesis are considered for the purpose of exactly compensating the effect of higher order truncated modes. Following this way, the analysis accuracy of the synthesized structure can be improved effectively with less computational effort. In order to show the accuracy and effectiveness of the proposed hybrid component mode synthesis(HCMS), numerical experiments were carried out for the models of a clamped-clamped beam. The results verified the effectiveness of the proposed method.

• 한국신재생에너지학회:학술대회논문집
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• 2011.05a
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• pp.74.2-74.2
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• 2011

A simulation study on synthesis gas process in GTL process was carried out in order to find optimum operation conditions for GTL (gas-to-liquid) pilot plant design. Optimum operating conditions for synthesis gas process were determined by changing reaction variables such as feed temperature and pressure. During the simulation, overall synthesis process was assumed to proceed under steady-state conditions. It was also assumed that physical properties of reaction medium were governed by RKS (Redlich-Kwong-Soave) equation. The effect of temperature and pressure on synthesis gas process $H_2$/CO ratio were mainly examined. Simulation results were also compared to experimental results to confirm the reliability of simulation model. Simulation results were reasonably well matched with experimental results.

• Biotechnology and Bioprocess Engineering:BBE
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• v.11 no.3
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• pp.258-261
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• 2006

The addition of zeolite particles enhances the stability of mRNA molecules in a cell-free protein synthesis system. When $20{\mu}g/{\mu}L$ of zeolite (Y5.4) is added to a reaction mixture of cell-free protein synthesis, a substantial increase in protein synthesis is observed. The stabilizing effect of zeolite is most dearly observed in an in vitro translation reaction directed by purified mRNA, as opposed to a coupled transcription and translation reaction. Upon the addition of zeolite in the in vitro translation reaction, the life span of the mRNA molecules is substantially extended, leading to an 80% increase in protein synthesis. The effect of zeolite upon the mRNA stability appears be strongly related to the cation exchange (potassium to sodium) reaction. Our results demonstrate the possibility of modifying this biological process using heterogeneous, non-biological substances in a cell-free protein synthesis system.

• Journal of the Korean Institute of Electrical and Electronic Material Engineers
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• v.25 no.6
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• pp.481-485
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• 2012

ZnO nanowires were synthesized by hydrothermal technique. Prepared synthesis aqueous solutions were preserved by preheating in autoclave type synthesis equipment with various preheating time of 1 h difference. ITO-coated corning glass substrates deposited with AZO seed layers were then inserted in the preheated synthesis aqueous solutions and ZnO nanowires were grown for 180 min at $90^{\circ}C$. Density, length and aspect ratio of the grown ZnO nanowires were investigated. Composition, structural and optical properties of the grown ZnO nanowires were analyzed. Characteristics of the ZnO nanowires were comparatively studied in relation with $Zn^{2+}$ ion concentration measured directly after the preheating of synthesis aqueous solution.