Browse > Article

Zeolite-Mediated Cation Exchange Enhances the Stability of mRNA during Cell-Free Protein Synthesis  

Kim, You-Eil (School of Chemical and Biological Engineering, Seoul National University)
Kim, Dong-Myung (Department of Fine Chemical Engineering and Chemistry, Chungnam National University)
Choi, Cha-Yong (School of Chemical and Biological Engineering, Seoul National University)
Publication Information
Biotechnology and Bioprocess Engineering:BBE / v.11, no.3, 2006 , pp. 258-261 More about this Journal
Abstract
The addition of zeolite particles enhances the stability of mRNA molecules in a cell-free protein synthesis system. When $20{\mu}g/{\mu}L$ of zeolite (Y5.4) is added to a reaction mixture of cell-free protein synthesis, a substantial increase in protein synthesis is observed. The stabilizing effect of zeolite is most dearly observed in an in vitro translation reaction directed by purified mRNA, as opposed to a coupled transcription and translation reaction. Upon the addition of zeolite in the in vitro translation reaction, the life span of the mRNA molecules is substantially extended, leading to an 80% increase in protein synthesis. The effect of zeolite upon the mRNA stability appears be strongly related to the cation exchange (potassium to sodium) reaction. Our results demonstrate the possibility of modifying this biological process using heterogeneous, non-biological substances in a cell-free protein synthesis system.
Keywords
zeolite; ion-exchange; sodium; potassium; cell-free protein synthesis; translation;
Citations & Related Records

Times Cited By Web Of Science : 4  (Related Records In Web of Science)
Times Cited By SCOPUS : 4
연도 인용수 순위
1 Hultin, T. and P. H. Näslund (1974) Effects of thallium (I) on the structure and functions of mammalian ribosomes. Chem. Biol. Interact. 8: 315-328   DOI   ScienceOn
2 Cahn, F. and M. Lubin (1978) Inhibition of elongation steps of protein synthesis at reduced potassium concentrations in reticulocytes and reticulocyte lysate. J. Biol. Chem. 253: 7798-7803
3 Fuchs, U., W. Stiege, and V. A. Erdmann (1997) Ribonucleolytic activities in the Escherichia coli in vitro translation system and in its separate components. FEBS Lett. 414: 362-364   DOI   ScienceOn
4 Kim, D.-M. and C.-Y. Choi (1996) A semicontinuous prokaryotic coupled transcription/translation system using a dialysis membrane. Biotechnol. Prog. 12: 645-649   DOI   ScienceOn
5 Ingle, C. A. and S. R. Kushner (1996) Development of an in vitro mRNA decay system for Escherichia coli: poly(A) polymerase I is necessary to trigger degradation. Proc. Natl. Acad. Sci. USA 93: 12926-12931
6 Doi , N., H. Takashima, M. Kinjo, K. Sakata, Y. Kawahashi, Y. Oishi, R. Oyama, E. Miyamoto-Sato, T. Sawasaki, Y. Endo, and H. Yanagawa (2002) Novel fluorescence labeling and high-throughput assay technologies for in vitro analysis of protein interactions. Genome Res. 12: 487-492   DOI
7 Kim, J.-E., E.-J. Kim, and T.-H. Park (2005) Enhanced production of recombinant protein in Escherichia coli using silkworm hemolymph. Biotechnol. Bioprocess Eng. 10: 353-356   과학기술학회마을   DOI   ScienceOn
8 Jewett, M. C., A. P. Oliveira, K. R. Patil, and J. Nielsen (2005) High-throughput transcriptome analysis in metabolic engineering. Biotechnol. Bioprocess Eng. 10: 385-399   과학기술학회마을   DOI   ScienceOn
9 Oh, M.-K., D. R. Scoles, and S.-M. Pulst (2005) DNA microarray analysis of immediate response to EGF treatment in rat schwannoma cells. Biotechnol. Bioprocess Eng. 10: 444-450   과학기술학회마을   DOI   ScienceOn
10 Naslund, P. H. and T. Hultin (1970) Effects of potassium deficiency on mammalian ribosomes. Biochim. Biophys. Acta 204: 237-247
11 Pratt, J. M. (1984) Coupled transcription-translation in prokaryotic cell-free system. pp. 179-209. In: B. D. Hames and S. J. Higgins (eds.). Transcription and Translation: A Practical Approach. IRL press, NY, USA
12 Kim, D.-M., Y.-E. Kim, and C.-Y. Choi (1996) Enhancement of protein synthesis with sodium ion in a cell-free system from Escherichia coli. J. Ferment. Bioeng. 82: 398-400   DOI   ScienceOn
13 Gurevich, V. V., I. D. Pokrovskaya, T. A. Obukhova, and S. A. Zozulya (1991) Preparative in vitro mRNA synthesis using SP6 and T7 RNA polymerases. Anal. Biochem. 195: 207-213   DOI   ScienceOn
14 Levine, H., M. R. Trindle, and K. Moldave (1966) Monovalent cation requirement for the aminoacyl transfer reaction in protein synthesis. Nature 211: 1302-1303   DOI   ScienceOn
15 Kawarasaki, Y., T. Kawai, H. Nakano, and T. Yamane (1995) A long-lived batch reaction system of cell-free protein synthesis. Anal. Biochem. 226: 320-324   DOI   ScienceOn
16 Pestka, S., R. Goorha, H. Rosenfeld, C. Neurath, and H. Hintikka (1972) Studies on transfer ribonucleic acidribosome complexes. XX. Peptidyl-puromycin synthesis on mammalian polyribosomes. J. Biol. Chem. 247: 4258-4263
17 Kim, D.-M., Y.-E. Kim, and C.-Y. Choi (1995) Effect of zeolites on protein synthesis in a cell-free system from Escherichia coli. Biotechnol. Lett. 17: 1043-1046   DOI
18 Jung, G.-Y., E.-Y. Lee, Y. Kim, B.-W. Jung, S.-H. Kang, and C.-Y. Choi (2000) Stabilization effect of zeolite on DHFR mRNA in a wheat germ cell-free protein synthesis system. J. Biosci. Bioeng. 89: 193-195   DOI   ScienceOn
19 Kim, D.-M., T. Kigawa, C.-Y. Choi, and S. Yokoyama (1996) A highly efficient cell-free protein synthesis system from Escherichia coli. Eur. J. Biochem. 239: 881-886   DOI   ScienceOn
20 Jermutus, L., L. A. Ryabova, and A. Plückthun (1998) Recent advances in producing and selecting functional proteins by using cell-free translation. Curr. Opin. Biotechnol. 9: 534-548   DOI   ScienceOn
21 Hultin, T. (1966) Factors influencing the puromycininduced release of protein from liver ribosomes. Biochim. Biophys. Acta 123: 561-573