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Analysis of Bacterials Community Structure in Leadchate-Contaminated Groundwater using Denaturing Gradient Gel Electrophoresis (Denaturing Gradient Gel Electrophoresis를 이용한 매립지 침출수로 오염된 지하수의 세균 군집 분석)

  • Kim Jai-Soo;Kim Ji-Young;Koo So-Yeon;Ko Kyung-Seok;Lee Sang-Don;Cho Kyung-Suk;Koh Dong-Chan
    • Microbiology and Biotechnology Letters
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    • v.34 no.2
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    • pp.166-173
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    • 2006
  • This research has been performed to clarify the relationship between hydrogeochemistry and bacterial community structure in groundwater contaminated with landfill leachate. We collected and analyzed samples from 5 sites such as leachate (KSG1-12), treated leachate (KSG1-16), two contaminated groundwaters (KSG1-07 and KSG1-08) and non-contaminated groundwater (KSG1-13). pH was 8.83, 8.04, 6.87, 6.87 and 6.53 in order; redox potential (Eh) 108, 202, 47, 200 and 154 mV; electric conductivity (EC) 3710, 894, 1223, 559 and 169.9 $\mu$S/cm; suspended solids (SS) 86.45, 13.74, 4.18, 0.24 and 11.91 mg/L. In KSG01-12, the ion concentrations were higher especially in $Cl^-$ and $HCO_3^-$ than other sites. The concentrations of Fe, Mn and $SO_4^{2-}$ were higher In KSG1-07 than in KSG1-08, and vise versa in $NO_3^{2-}$. In the comparison of DGGE fingerprint patterns, the similarity was highest between KSG1-13 and KSG1-16 (57.2%), probably due to common properties like low or none contaminant concentrations. Otherwise KSG1-08 showed lowest similarities with KSG1-13 (25.8%) and KSG1-12 (27.6%), maybe because of the degree of contamination. The most dominant bacterial species in each site were involved in $\alpha$-Proteobacteria (55.6%) in KSG1-12, $\gamma$-Proteobacteria (50.0%) in KSG1-16, $\beta$-Proteobacteria (66.7%) in KSG1-07, $\gamma$-Proteobacteria (54.5%) in KSG1-08 and $\beta$-Proteobacteria (36.4%) in KSG1-13. These results indicate that the microbial community structure might be changed according to the flow of leachate in grounderwater, implying changes in concentrations of pollutants, available electron accepters and/or other environmental conditions.

Spatial Characteristics of Pollutant Concentrations in the Streams of Shihwa Lake (시화호 유입하천의 수질오염물질 농도에 관한 연구)

  • Jang, Jeong-Ik;Han, Ihn-Sup;Kim, Kyung-Tae;Ra, Kong-Tae
    • Journal of Korean Society of Environmental Engineers
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    • v.33 no.4
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    • pp.289-299
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    • 2011
  • We studied the characteristics of pollutant concentrations in 9 streams that flow into Shihwa Lake in order to provide the scientific data for effective implementation of total pollution loads management system (TPLMS) of the Lake. Suspended solid (SS), chemical oxygen demand (COD), dissolved nutrients ($NO_2$, $NO_3$, $NH_4$, $PO_4$ and $SiO_2$), total phosphorus (TP) and total nitrogen (TN) in stream water from industrial complexes, urban and agricultural regions were determined. Pollutant concentrations in December were higher than that in other sampling periods. COD concentration from industrial complex region with average of 12.6 mg/L was 2 times higher those from urban region (6.6 mg/L) and agricultural region (5.9 mg/L). TP concentration from industrial region also showed higher concentration than other regions. TN concentration in stream water was 5.89 mg/L for industrial region, 3.02 mg/L for urban region and 5.27 mg/L for agricultural region, respectively, suggesting inflow of TN due to fertilizer usage in agricultural field. Relative percentage of nitrogen compounds in TN follows the sequence: $NH_4$ (35.1%) > $NO_2$ (20.0%) > DON (22.8%) > PON (8.9%) > $NO_2$ (3.2%). Concentrations of dissolved nutrients, TP and TN in stream water were 3.2~37.2 times higher than that in Shihwa Lake seawater, therefore large amount of pollutants may be directly entered into Shihwa Lake without any treatment. For Gunja stream of industrial region, pollutants at midstream showed relatively higher concentration compared to upstream and downstream. It is necessary to manage the illegal discharging of sewage and waste water. Our results provide valuable informations on the estimation and reduction of total pollutant loads in the process of establishing adequately strategic and implemental plan of Shihwa Lake TPLMS.

Long-term Variation in Ocean Environmental Conditions of the Northern East China Sea (동중국해 북부해역의 해양환경 장기변동)

  • Yoon, Sang Chol;Youn, Suk Hyun;Whang, Jae Dong;Suh, Young Sang;Yoon, Yi Yong
    • Journal of the Korean Society for Marine Environment & Energy
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    • v.18 no.3
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    • pp.189-206
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    • 2015
  • The present study was conducted to investigate the oceanic characteristics of the northern East China Sea through identification of long-term variation patterns of oceanic environment factors, for the objective of gaining understanding of oceanic environment characteristics of the northern waters of East China Sea, which closely influence the oceanic environments of waters nearby South Korea. The study methodology included the use of oceanographic data (water temperature, salinity, dissolved oxygen, nutrients, and chlorophyll-a) on the northern East China Sea from the Korea Oceanographic Data Center (KODC), collected by season for 20 years between 1995 and 2014. Moreover, for the study on the distribution of nutrients, chlorophyll-a. The main water masses that affected the northern East China Sea during the study period were classified as Changjiang diluted water (CDW), Tiawan current warm water (TCWW), Yellow Sea cold water (YSCW), and Kuroshio source water (KW). The forces of CDW and TCWW that forms on the surface and sub-surface layers had weakened for 20 years and the force of KW that forms on the intermediate layer showed a distinctively decreasing trend. However, YSCW showed a trend of expanding its force. Phosphate and silicate exhibited a decreasing tendency and phosphate showed a pattern of being depleted on the surface layer after 2009. It is determined that one of the reasons for this is the concentration of nutrients introduced through CDW and TCWW being too low. The concentration of chlorophyll-a exhibited an increasing tendency during the study period, the reasons for which are determined to be the influences of increase in water temperature, supply of nutrients via YSCW, and increases in light transmission from decrease in suspended solid due to the construction of the Three Gorges Dam.

Effect of the Application of a Suspended and a Mixing-in-Pipe Type Aerator on the Liquid Fertilization of Pig Manure Slurry (현수, 배관 내 혼합 폭기방식 적용이 돼지분뇨 슬러리의 액상 비료화에 미치는 영향)

  • Jeong, Kwang Hwa;Kim, Jung-Kon;Khan, Modabber Ahmed;Kwag, Jung-Hoon;Han, Duk-Woo
    • Journal of the Korea Organic Resources Recycling Association
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    • v.22 no.4
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    • pp.62-71
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    • 2014
  • Three types of diffuser systems were manufactured and applied to investigate the effect of liquid fertilization of pig manure slurry by application of aeration processes. In the first type reactor, commonly used diffuser system, which diffuse air upward by diffusing aerator fixed at the bottom of the reactor is installed. In case of the second type, air diffuser is installed 10 cm above of the bottom of a reactor. In the third type reactor, the venturi-type air diffuser is installed at circulation pipe, which return pig slurry in the reactor(mixing-in-pipe process). The pig manure slurry separated to solid/liquid was flowed into the experimental reactor, and left as it for one week to precipitate solids. The concentration of organic matter, T-N, T-P and BOD in the raw pig manure slurry flowed into the reactor of bottom-fixed type aeration process were 1.82%, 4,400 mg/L, 360 mg/L and 13,542 mg/L, respectively. After aeration the concentration of organic matters, T-N, T-P and BOD in the slurry were 2.01%, 4,400 mg/L, 420 mg/L and 16,824 mg/L, respectively. The concentration of organic matter, T-N, T-P and BOD in the mixing-in-pipe type changed from 1.58%, 3,700 mg/L, 260 mg/L and 15,735 mg/L to 1.96%, 4,000 mg/L, 340 mg/L, and 18,098 mg/L, respectively. Changes of the concentration of organic matter, T-N, T-P and BOD of the pig manure slurry collected from the middle layers of two aeration reactors; bottom aeration process and the mixing-in-pipe process, were 10.4%, 0%, 16.7% and 24.2% and 24.0%, 8.1%, 30.8% and 15.0%, respectively. The thickness of foam layer generated on the surface of pig manure slurry in aeration tank was thinner in mixing-in-pipe reactor than bottom-fixed type aeration reactor.

Reduction of Pollutant Load by Small Pond in a Rice Paddy Applied with Pig Manure Compost (돈분퇴비가 시용된 논의 양분유출 저감을 위한 저류지 효과)

  • Kim, Min-Kyeong;Kim, Myung-Hyun;Choi, Soon-Kun;Cho, Kwang-Jin;Hong, Seong-Chang;Jung, Goo-Bok;So, Kyu-Ho
    • Journal of the Korea Organic Resources Recycling Association
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    • v.22 no.4
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    • pp.21-27
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    • 2014
  • Pig slurry has been considered as environmental waste to be treated in an appropriate manner. Moreover, water-born pollution loads by agricultural non-point source(NPS) pollution are expected to become intensified due to ongoing precipitation change. This study was conducted to develop a best management practice to reduce NPS pollution load by agricultural activity with pig manure compost. An eco-friendly way, small drainage pond, was suggested in this study to avoid direct drainage of agricultural runoffs and eventually reduce the amount of pollutants discharged into the surrounding aqua-environment. A small pond($12m^2$) was constructed at the corner of a rice paddy field($17,15m^2$) located in Suwon, Korea. Water was allowed to drain only via a small drainage pond. Sampling was repeatedly made at two locations, one from an entrance and the other from an exit of a pond, during the rice cultivation period(May to October, 2013). Generally, sampling was made only when runoff water drained through a pond, such as during and/or after rain(irrigation). The water quality analysis showed that all quality parameters(SS, $COD_{Mn}$, T-N, and T-P) were improved as water passed through the pond. The amount of runoff water was reduced by 67.8%. Suspended solids and $COD_{Mn}$ concentrations were reduced by 79.8% and 71.9%, respectively. In case of T-N and T-P amounts, the reduction rates were 73.6% and 74.9%, respectively. Our data implies that agricultural NPS pollution from rice paddy fields with pig manure-based fertilizer can be effectively managed when an appropriate drainage water management practice is imposed.

The Effect of Variations in the Tsushima Warm Currents on the Egg and Larval Transport of Anchovy in the Southern Sea of Korea (한국 남해의 대마난류 변동이 멸치 난$\cdot$자어의 연안역 수송에 미치는 영향)

  • CHOO Hyo-Sang;KIM Dong-Soo
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.31 no.2
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    • pp.226-244
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    • 1998
  • The relationship between the transport of eggs and larvae of Anchovy (Engraulis japonica) and the oceanic condition in the southern sea of Korea was examined on August and November 1996. In summer (August), when the Tsushima Warm Current is strong near to the coast, the warm waters such as warm streamers from the Tsushima Warm Current intrude into the coastal area, and cyclonic circulations are formed. The warm water intrusions also generate wakes around Komun Island, Sori Island and Koje Island. In the coastal area where the warm water intrusions occur, the nutrients, dissolved oxygen, suspended solid and chlorophyll are concentrated in probably relation to the upwelling concerned with this warm streamer and/or the wakes. Anchovy eggs and larvae are transported to the coastal area by the cyclonic circulations. The hatching and growth of anchovy larvae are increased because of high primary production in the cyclonic circulations. However, as the amount of Copepods which are a main food for anchovy larvae decrease in the coastal area, anchovy larvae seem to move to the Isushima Warm Water area for seeking a prey. In autumn (November), the Tsushima Warm Current is far away from the coast. In this season the warm water intrusions almost disappear, and the small scaled frontal eddies are formed between the coastal water and the Tsushima Warm Water. As the surface water moves towards offshore, few anchovy eggs and larvae were sampled in the survey area. Chemical and biological substances are concentrated in the leftdown sides of the small scaled frontal eddies because of eddy formation.

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The Study on Ozone Treatment of Wasting Activated Sludge for VFA Production and Reuse as Carbon Source for Phosphorus Release (잉여슬러지의 오존분해에 따른 VFA의 생성 및 인 방출을 위한 탄소원으로의 재이용 가능성에 관한 연구)

  • Ko, Eun-Taek;Cho, Jin-Woo;Park, Eun-Young;Ahn, Kyu-Hong
    • Journal of Korean Society of Environmental Engineers
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    • v.27 no.10
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    • pp.1052-1057
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    • 2005
  • Recently, the ozone treatment of wasting activated sludge has become one of the effective and feasible process for the sludge reduction. The objective of this study is to investigate the availability of ozonized wasting sludge on external carbon sources 13r phosphorus release. Experiment results showed that the ozone treatment of activated sludge could produce a large amount of VFA such as acetic acid and isobutyric acid. For example, 50.24 mg/L acetic acid was produced with the ozone dose of 0.05 g $O_3/g$ SS, and 123.56 mg/L acetic acid with 0.5 g $O_3/g$ SS. The higher ozone dose was applied, the more VFA was produced from sludge reduction into a limited point. Finally, using ozonated sludge as only carbon source, the batch experiment, to measure phosphorus release rate in anaerobic condition were performed. The specific phosphorus release rates were investigated as 0.94, 1.37, 1.48, 1.68 mg P/g VSS/hr with ozone dose of 0.05, 0.1, 0.2, 0.5 g $O_3/g$ SS, respectively. Considering the degree of mineralization, VFA production, phosphorus release rate, and economical aspect, the optimal ozone dose for sludge reduction and using carbon sources ranged from 0.05 to 0.1 g $O_3/g$ SS.

Study on the Characteristics and Non-point Source Pollution Loads in Stormwater Runoff of Shihwa Lake (시화호 유역 비점오염물질의 유출특성 및 부하량 연구)

  • Ra, Kong-Tae;Kim, Kyung-Tae;Kim, Joung-Keun;Bang, Jae-Hyun;Lee, Jung-Moo;Kim, Sung-Keun;Kim, Eun-Soo;Yun, Min-Sang;Cho, Sung-Rok
    • Journal of the Korean Society for Marine Environment & Energy
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    • v.14 no.1
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    • pp.40-50
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    • 2011
  • We study the characteristic and total flux of non-point pollutants such as total suspended solids (TSS), chemical oxygen demand (COD), dissolved nutrients, total phosphorus (TP) and total nitrogen (TN) in the storm water runoff from urban streams and sewer outlets of Banweol Industrial Complex around Shihwa Lake. The concentrations of non-point pollutants were generally increased with increasing of the duration and intensity of rainfall. Mean TSS concentration of Ansan stream was higher than that of sewer outlets but mean concentrations of COD, TP and TN were approximately 2~5 times higher of sewer outlet than of urban stream. TSS showed statistically positive relationships with COD and TP but it had negative correlation with dissolved nutrients. There was a significant correlation between total flux of non-point pollutants in the storm water runoff and total basin area of each sewer outlet, showing that the highest runoff flux was observed at 3rd sewer outlet which represents the largest basin area from Banweol industrial complex. Total runoff fluxes for TSS, COD, TP and TN in this study were 187,536 kg, 17,118 kg, 922 kg, 13,519 kg, respectively. Given the basin area of sewer outlet in Banweol industrial complex which corresponds only 3% from total catchment area around Shihwa Lake, enormous amount of non-point pollutants will be entered into Shihwa Lake without any treatment. It is necessary to manage and reduce of various non-point sources and pollutants because the runoff of nonpoint pollutants during storm events should be deteriorating the water quality of Shihwa Lake. Our results provides useful informations on the development of best managements practices (BMPs) for effective implementation of total pollution loads management system of Shihwa Lake.

Modification of Endothelium on Contractile Response of Brain Vessels to Contracting Agents (혈관 수축제의 뇌혈관 수축반응에 대한 혈관근 내피세포의 역할)

  • Kook, Young-Johng;Baik, Yung-Hong;Kim, Jong-Keun;Choi, Bong-Kyu;Choi, Soo-Hyung;Kim, Yung-In
    • The Korean Journal of Pharmacology
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    • v.24 no.2
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    • pp.203-216
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    • 1988
  • To delineate the mechanisms of vasoconstriction and vasodilation in cerebral arteries the effects of some vasoconstrictors and calcium antagonists on the basilar artery (BA) and arterial circle of Willis (WC) were examined and also the role of endothelium in the action of these drugs was investigated in pigs, cats and rabbits. In pig cerebral arteries, dose-dependent contractile responses were elicited by KCI, histamine, 5-hydroxytryptamine (5-HT) and angiotensin, but norepinephrine (NE), phenylephrine (PE) and epinephrine (EP) elicited dose-dependent contractions only under pretreatment with propranolol 10-6 M. The magnitudes of maximal contractile effects of these drugs were different from each other, and 5-H~ was the largest and angiotensin the smallest. Some calcium antagonists dose-dependently inhibited KCI (35 mM)-induced contraction and the order of potency in inhibiting the contraction was nifedipine > > diltiazem > flunarizine > oxybutynin > isosorbide dinitrate (ISDN) > glyceryl trinitrate. 5-HT (10-6 M)-induced contraction was dosedependently inhibited by nifedipine but slightly inhibited by diltiazem and ISDN. In rings with intact endothelium, KCI (35 mM)-induced contraction was not affected by acetylcholine (ACh) but $PGF_{2{\alpha}}$ (lO-SM)-induced contraction was dose-dependently relaxed by ACh and adenosine. This endothelium-dependent relaxation was not affected by nifedipine (l0-6M)-pretreatment but markedly inhibited by methylene blue (50,uM)-pretreatment. In the porcine arterial rings without endothelium, ACh had no effect or even contracted the $PGF_{2{\alpha}}-induced$ contraction. However, the dosedependent relaxing effect of ACh appeared when the deendothelized porcine ring and rabbit thoracic aorta with intact endotheli urn were simultaneously suspended into a bath and this relaxing effect was also inhibited by methylene blue-pretreatment. In cat cerebral arteries, 5-HT and NE elicited dose-dependent contractile responses and ACh also produced dose-dependent contraction regardless of the existence of endothelium. ACh-induced contraction was most prominent. 5-HT (IO-SM)induced contraction was not relaxed but contracted additionally by ACh even in the intact endothelial ring. In rabbit cerebral arteries, 5-HT and NE elicited dose-dependent contractile responses and 5-HT-induced contraction was more prominent. In the intact endothelial preparations, 5-HT (lO-s M)-induced contraction was markedly relaxed by the addition of ACh( IO-SM) and this endothelium-dependent relaxing effect was inhibited by atropine (l0-7M)-pretreatment but notaffected by diltiazem (l0-6M)-pretreatment. These results suggest that ACh elicits endotheliumdependent relaxing effect mediated by muscarinic receptors in cerebral arteries of pig and rabbit, and that ACh acts as vasoconstrictor in cat cerebral artery.

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Estimation of Chlorophyll-a Concentrations in the Nakdong River Using High-Resolution Satellite Image (고해상도 위성영상을 이용한 낙동강 유역의 클로로필-a 농도 추정)

  • Choe, Eun-Young;Lee, Jae-Woon;Lee, Jae-Kwan
    • Korean Journal of Remote Sensing
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    • v.27 no.5
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    • pp.613-623
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    • 2011
  • This study assessed the feasibility to apply Two-band and Three-band reflectance models for chlorophyll-a estimation in turbid productive waters whose scale is smaller and narrower than ocean using a high spatial resolution image. Those band ratio models were successfully applied to analyzing chlorophyll-a concentrations of ocean or coastal water using Moderate Imaging Spectroradiometer(MODIS), Sea-viewing Wide Field-fo-view Sensor(SeaWiFS), Medium Resolution Imaging Spectrometer(MERIS), etc. Two-band and Three-band models based on band ratio such as Red and NIR band were generally used for the Chl-a in turbid waters. Two-band modes using Red and NIR bands of RapidEye image showed no significant results with $R^2$ 0.38. To enhance a band ratio between absorption and reflection peak, We used red-edge band(710 nm) of RapidEye image for Twoband and Three-band models. Red-RE Two-band and Red-RE-NIR Three-band reflectance model (with cubic equation) for the RapidEye image provided significance performances with $R^2$ 0.66 and 0.73, respectively. Their performance showed the 'Approximate Prediction' with RPD, 1.39 and 1.29 and RMSE, 24.8, 22.4, respectively. Another three-band model with quadratic equation showed similar performances to Red-RE two-band model. The findings in this study demonstrated that Two-band and Three-band reflectance models using a red-edge band can approximately estimate chlorophyll-a concentrations in a turbid river water using high-resolution satellite image. In the distribution map of estimated Chl-a concentrations, three-band model with cubic equation showed lower values than twoband model. In the further works, quantification and correction of spectral interferences caused by suspended sediments and colored dissolved organic matters will improve the accuracy of chlorophyll-a estimation in turbid waters.