• Title/Summary/Keyword: Successful fusion

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Identification of Certain Sequences in the 3rd Cytoplasmic Loop of$D_4$ Dopamine Receptor that Suppress the Bacterial Expression

  • Cheong, Ji-Sook;Kim, Ae-Young;Kim, Kyeong-Man
    • Archives of Pharmacal Research
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    • v.19 no.4
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    • pp.275-279
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    • 1996
  • To study the functional roles of dopamine receptors, we decided to raise antibodies against these proteins. To make antigen, we expressed the whole 3rd cytoplasmic loop of dopamine receptors in a fusion protein with glutathione-S-transferase (GST). $For D_2\; and\; D_3$ receptors, it was successful to express and purify fusion proteins for the whole 3rd cytoplasmic loops. However, we could not express the fusion protein for the whole 3rd cytoplasmic loop of $D_4$ dopamine receptor in the bacteria. To study the causes that prevent the bacterial expression of the GST-fusion protein of the 3rd cytoplasmic loop of $D_4$ dopamine receptor, we conducted more detailed studies on $D_4$ dopamine receptor. To locate the region which prevents bacterial expression, we made sequential constructs in the 3rd cytoplasmic loop decreasing the size step by step, and confirmed their expressions in the SDS PAGE. It was found that certain regions of 3rd cytoplasmic loop of $D_4$ dopamine receptor, located in N-terminal side of the 3rd cytoplasmic loop of $D_4$ dopamine receptor suppress the bacterial expression of fusion protein.

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MULTI-SENSOR DATA FUSION FOR FUTURE TELEMATICS APPLICATION

  • Kim, Seong-Baek;Lee, Seung-Yong;Choi, Ji-Hoon;Choi, Kyung-Ho;Jang, Byung-Tae
    • Journal of Astronomy and Space Sciences
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    • v.20 no.4
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    • pp.359-364
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    • 2003
  • In this paper, we present multi-sensor data fusion for telematics application. Successful telematics can be realized through the integration of navigation and spatial information. The well-determined acquisition of vehicle's position plays a vital role in application service. The development of GPS is used to provide the navigation data, but the performance is limited in areas where poor satellite visibility environment exists. Hence, multi-sensor fusion including IMU (Inertial Measurement Unit), GPS(Global Positioning System), and DMI (Distance Measurement Indicator) is required to provide the vehicle's position to service provider and driver behind the wheel. The multi-sensor fusion is implemented via algorithm based on Kalman filtering technique. Navigation accuracy can be enhanced using this filtering approach. For the verification of fusion approach, land vehicle test was performed and the results were discussed. Results showed that the horizontal position errors were suppressed around 1 meter level accuracy under simulated non-GPS availability environment. Under normal GPS environment, the horizontal position errors were under 40㎝ in curve trajectory and 27㎝ in linear trajectory, which are definitely depending on vehicular dynamics.

Load Sharing Mechanism Across Graft-Bone Interface in Static Cervical Locking Plate Fixation

  • Han, In-Ho;Kuh, Sung-Uk;Chin, Dong-Kyu;Jin, Byung-Ho;Cho, Yang-Eun;Kim, Keun-Su
    • Journal of Korean Neurosurgical Society
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    • v.45 no.4
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    • pp.213-218
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    • 2009
  • Objective : This study is a retrospective clinical study over more than 4 years of follow up to understand the mechanism of load sharing across the graft-bone interface in the static locking plate (SLP) fixation compared with non-locking plate (NLP). Methods : Orion locking plates and Top non-locking plates were used for SLP fixation in 29 patients and NLP fixation in 24 patients, respectively. Successful interbody fusion was estimated by dynamic X-ray films. The checking parameters were as follows : screw angle (SA) between upper and lower screw, anterior and posterior height of fusion segment between upper and lower endplate (AH & PH), and upper and lower distance from vertebral endplate to the end of plate (UD & LD). Each follow-up value of AH and PH were compared to initial values. Contributions of upper and lower collapse to whole segment collapse were estimated. Results : Successful intervertebral bone fusion rate was 100% in the SLP group and 92% in the NLP group. The follow-up mean value of SA in SLP group was not significantly changed compared with initial value, but follow-up mean value of SA in NLP group decreased more than those in SLP group (p=0.0067). Statistical analysis did not show a significant difference in the change in AH and PH between SLP and NLP groups (p>0.05). Follow-up AH of NLP group showed more collapse than PH of same group (p=0.04). The upper portion of the vertebral body collapsed more than the lower portion in the SLP fixation (p=0.00058). Conclusion : The fused segments with SLP had successful bone fusion without change in initial screw angle, which was not observed in NLP fixation. It suggests that there was enough load sharing across bone-graft interface in SLP fixation.

Development of Multi-sensor Image Fusion software(InFusion) for Value-added applications (고부가 활용을 위한 이종영상 융합 소프트웨어(InFusion) 개발)

  • Choi, Myung-jin;Chung, Inhyup;Ko, Hyeong Ghun;Jang, Sumin
    • Journal of Satellite, Information and Communications
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    • v.12 no.3
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    • pp.15-21
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    • 2017
  • Following the successful launch of KOMPSAT-3 in May 2012, KOMPSAT-5 in August 2013, and KOMPSAT-3A in March 2015 have succeeded in launching the integrated operation of optical, radar and thermal infrared sensors in Korea. We have established a foundation to utilize the characteristics of each sensors. In order to overcome limitations in the range of application and accuracy of the application of a single sensor, multi-sensor image fusion techniques have been developed which take advantage of multiple sensors and complement each other. In this paper, we introduce the development of software (InFusion) for multi-sensor image fusion and valued-added product generation using KOMPSAT series. First, we describe the characteristics of each sensor and the necessity of fusion software development, and describe the entire development process. It aims to increase the data utilization of KOMPSAT series and to inform the superiority of domestic software through creation of high value-added products.

Intelligent System based on Command Fusion and Fuzzy Logic Approaches - Application to mobile robot navigation (명령융합과 퍼지기반의 지능형 시스템-이동로봇주행적용)

  • Jin, Taeseok;Kim, Hyun-Deok
    • Journal of the Korea Institute of Information and Communication Engineering
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    • v.18 no.5
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    • pp.1034-1041
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    • 2014
  • This paper propose a fuzzy inference model for obstacle avoidance for a mobile robot with an active camera, which is intelligently searching the goal location in unknown environments using command fusion, based on situational command using an vision sensor. Instead of using "physical sensor fusion" method which generates the trajectory of a robot based upon the environment model and sensory data. In this paper, "command fusion" method is used to govern the robot motions. The navigation strategy is based on the combination of fuzzy rules tuned for both goal-approach and obstacle-avoidance. We describe experimental results obtained with the proposed method that demonstrate successful navigation using real vision data.

Comparisons of internal self-field magnetic flux densities between recent Nb3Sn fusion magnet CICC cable designs

  • Kwon, S.P.
    • Progress in Superconductivity and Cryogenics
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    • v.18 no.3
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    • pp.10-20
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    • 2016
  • The Cable-In-Conduit-Conductor (CICC) for the ITER tokamak Central Solenoid (CS) has undergone design change since the first prototype conductor sample was tested in 2010. After tests showed that the performance of initial conductor samples degraded rapidly without stabilization, an alternate design with shorter sub-cable twist pitches was tested and discovered to satisfy performance requirements, namely that the minimum current sharing temperature ($T_{cs}$) remained above a given limit under DC bias. With consistent successful performance of ITER CS conductor CICC samples using the alternate design, an attempt is made here to revisit the internal electromagnetic properties of the CICC cable design to identify any correlation with conductor performance. Results of this study suggest that there may be a simple link between the $Nb_3Sn$ CICC internal self-field and its $T_{cs}$ performance. The study also suggests that an optimization process should exist that can further improve the performance of $Nb_3Sn$ based CICC.

Fuctionalization of SBA-16 Mesoporous Materials with Cobalt(III) Cage Amine Complex

  • Han, Sang-Cheol;Sujandi, Sujandi;Park, Sang-Eon
    • Bulletin of the Korean Chemical Society
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    • v.26 no.9
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    • pp.1381-1384
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    • 2005
  • Surface modification of tridimensional cubic mesoporous silica, SBA-16, was investigated with pendant arm functionalized cobalt diaminosarcophagine (diAMsar) cage complex which covalently grafted onto the silica surface through the silication with sylanol group. The spectroscopic results showed that the mesoporous structure was preserved under the $[Co(diAMsar)]^{3+}$ grafting reaction condition. Successful grafting prevented the cobalt diAMsar cage from leaching out from the SBA-16 support.

Thermoplastic Fusion Bonding of UV Modified PMMA Microfluidic Devices (UV 개질된 PMMA 미세유체 장치의 열가소성 폴리머 용융 접합)

  • Park, Taehyun
    • Journal of the Korean Society for Precision Engineering
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    • v.31 no.5
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    • pp.441-449
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    • 2014
  • Thermoplastic fusion bonding is widely used to seal polymer microfluidic devices and optimal bonding protocol is required to obtain a successful bonding, strong bonding force without channel deformation. Besides, UV modification of the PMMA (poly-methyl methacrylate) is commonly used for chemical or biological application before the bonding process. However, study of thermal bonding for the UV modified PMMA was not reported yet. Unlike pristine PMMA, the optimal bonding parameters of the UV modified PMMA were $103^{\circ}C$, 71 kPa, and 35 minutes. A very low aspect ratio micro channel (AR=1:100, $20{\mu}m$ depth and $2000{\mu}m$ width) was successfully bonded (over 95%, n>100). Moreover, thermal bonding of multi stack PMMA chips was successfully demonstrated in this study. The results may applicable to fabricate a complex 3 dimensional microchannel networks.

Image Fusion of High Resolution SAR and Optical Image Using High Frequency Information (고해상도 SAR와 광학영상의 고주파 정보를 이용한 다중센서 융합)

  • Byun, Young-Gi;Chae, Tae-Byeong
    • Journal of the Korean Society of Surveying, Geodesy, Photogrammetry and Cartography
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    • v.30 no.1
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    • pp.75-86
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    • 2012
  • Synthetic Aperture Radar(SAR) imaging system is independent of solar illumination and weather conditions; however, SAR image is difficult to interpret as compared with optical images. It has been increased interest in multi-sensor fusion technique which can improve the interpretability of $SAR^{\circ\circ}$ images by fusing the spectral information from multispectral(MS) image. In this paper, a multi-sensor fusion method based on high-frequency extraction process using Fast Fourier Transform(FFT) and outlier elimination process is proposed, which maintain the spectral content of the original MS image while retaining the spatial detail of the high-resolution SAR image. We used TerraSAR-X which is constructed on the same X-band SAR system as KOMPSAT-5 and KOMPSAT-2 MS image as the test data set to evaluate the proposed method. In order to evaluate the efficiency of the proposed method, the fusion result was compared visually and quantitatively with the result obtained using existing fusion algorithms. The evaluation results showed that the proposed image fusion method achieved successful results in the fusion of SAR and MS image compared with the existing fusion algorithms.

Outcome Analysis of the Patients with and without Anterior Plating in Multi-Level Degenerative Cervical Diseases (다분절 퇴행성 경추질환에서의 전방 금속판 사용 유무에 따른 환자의 예후분석)

  • Kim, Sang Woo;Kim, Sung Min;Shin, Dong Ik;Cho, Yong Jun;Shim, Young Bo;Choi, Sun Kil
    • Journal of Korean Neurosurgical Society
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    • v.30 no.12
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    • pp.1369-1374
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    • 2001
  • Objectives : The rates of pseudarthrosis for two- and three level fusion have been reported to be 17-63 and 50% without anterior cervical plating. The purpose of this study is to assess the effects of anterior cervical plating in the treatment of multilevel degenerative cervical disease such mostly the additional risks associated with hardware implants and its benefits, fusion rate and radiographic results, and clinical outcomes. Methods : Forty-seven patients who underwent operations between 1993 and 1997 were retrospectively reviewed. The technique for operation was same for both groups(Smith Robinson with autologous iliac bone graft). Group I consisted of 35 consecutive patients treated with anterior cervical decompression and fusion with anterior cervical plate fixation. Group II consisted of 12 consecutive patients treated without plate fixation. We compared clinical outcomes by Prolo score, radiographic results in the rate of fusion, cervical lordosis by Gore angle, disc height by Farfan method, and surgical complications between two groups. Results : The favorable clinical outcomes(excellent and good) by Prolo score were observed with the use of anterior cervical plate fixation(89% vs 75%). The successful fusion rate of multilevel cervical fusion was as seen with anterior cervical plate fixation(97% vs 75%). The overall graft complication rate in multilevel fusions was decreased, with anterior cervical plate fixation, and the hardware related complications were relatively minimal without serious consequences. Conclusion : Anterior cervical plate fixation in the treatment of multilevel cervical disorders is an effective stabilizing method which provides increased bony fusion rate, decreased graft complications, maintained cervical lordosis, early mobilization of the these patients without serious hardware related complications.

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