• Biomedical Science Letters
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• v.10 no.4
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• pp.415-420
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• 2004

Fibrinolytic substance was purified from the Wooltalikong (Phaseolus ssp.), using DEAE-cellulose chromatography, Sephadex G-150 gel-filtration, and FPLC gel-filtration. The substance has a molecular weight of 5262.70 Da as measured by MALD-TOF mass spectrometry. It has a pH optimum at pH 6.0. The fibrinolytic activity of purified substance was inhibited by EDTA and 1,10-phenanthroline and slightly decreased by PMSF and pepstatin A. It shows the maximum fibrinolytic activity at 40℃ and the substance was stable up to 50℃. The activity of the substance was increased by Zn/sup 2+/ and was totally inhibited by Hg/sup 2+/. This study revealed that Wooltalikong could be a good source of fibrinolytic products due to its small molecular size and heat-resistant ability.

• The Journal of Korean Physical Therapy
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• v.15 no.1
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• pp.9-25
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• 2003

The purpose of this study was to evaluate the effect of therapeutic ultrasound after muscle contusion injury by observed immunoreactivity of substance-P that plays an important role in pain transmission. Ultrasound irradiation(1MHz, 1W/$cm^{2}$ continuous mode, treatment time 5 min) was applied through water submersion technique to 1 limb daily by kept off 5cm from muscle belly of gastrocnemius. The result of this study were as follows. 1. The substance-P was expressed in lamina I and II of dorsal horn of spinal cord, also in lamina IV and around of central cannel of spinal cord. Experimental group was lower expressed than control group with the exception of 1 days. 2. The substance-P immunoreactivity was decreased for 5 days together in lumbar and sacral region of all groups, expecially experimental group was rapidly. These data suggest therapeutic ultrasound may stimulate pain relief by diminish of substance-P in dorsal horn of spinal cord.

• Journal of Plant Biotechnology
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• v.32 no.2
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• pp.139-144
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• 2005

This experiment was conducted to identify the effect of several plant growth retardants on endogenous ABA-like substance content and GA-like substance activity in seedlings of Sedirea japonica cultured in vitro. When seedlings of Sedirea japonica were treated with low concentration of 0.05 mg/L Uniconazole, 0.1 mg/L Ancymidol and 0.3 mg/L Paclobutrazol, the content of ABA-like substances of the leaf was lower than that of the control. However, the activity of GA-like substances was similar or higher in treated seedlings. In the mid and high concentrations of three kinds of growth retardants, the ABA-like substance content was increased, but GA-like substance activity was inhibited. The content of ABA-like substances in the root was lower in 0.05 and 0.2 mg/L Uniconazole, 0.2 mg/L Ancymidol and 0.1 mg/L Paclobutrazol treatments than that of the control, but in the mid and high concentration treatments, the content was increased. GA-like substance activity in low concentration was increased but in the mid and high concentration, the activity was inhibited compared with low concentration treatment.

• The Korean Journal of Physiology
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• v.28 no.1
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• pp.51-59
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• 1994

This study was carried out to elucidate the excitatory mechanisms of Substance P in the antral circular muscle, using isometric contraction recording, conventional microelectrode method and whole-cell patch clamp technique. Substance P produced tonic and phasic contractions in a dose-dependent manner and depolarized membrane potential with increased amplitude of slow waves in muscle strips. Voltage-dependent $Ca^{2+}$ currents were increased by the application of Substance P from a holding potential of -60mV to 50mV in 10mV steps and this effect was blocked by the addition of an antagonist. Also Substance P increased transient and spontaneous oscillatory $K^+$ outward currents. The enhanced outward currents were abolished by apamin in dispersed single cells. These results suggest that the depolarization of membrane potential by Substance P activates voltage-dependent $Ca^{2+}$ channels, which represents an excitatory response in the antral circular muscle and led to an increase in $Ca^{2+}\;activated\;K^+\;currents$.

• The Korean Society of Law and Medicine
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• v.14 no.1
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• pp.11-36
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• 2013

Korea has been recognized as a safe country about the substance abuse, but now is not safe country because there are over 10 thousand drug related offenses in a year since 1999. Seventy percent of them are drug abusers, so Korea government must have stronger political strategies about the prevention and the treatment of the drug abuse. Till now Korea government has focused on the imprisonment rather than the treatment for the drug abusers. So the relapse rates are over 40% among the incarcerated drug abusers. Now we need more treatment strategies and development of treatment methods to decrease the relapse rates. Substance abuse is a brain disease with a chronic relapsing nature and has a bio-psycho-social etiology. So treatment process has three steps, motivation enhancement treatment for substance-free lifestyle, rehabilitation aimed at maximizing multiple aspects of life functioning, and relapse prevention. To improving the treatment strategies the Government must execute the national survey about the substance abuse, enforce a law to operate the drug court and to sentence a treatment order with a probation, and establish a national addiction institute for study and education about the substance abuse.

• Bulletin of the Korean Chemical Society
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• v.35 no.1
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• pp.158-164
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• 2014

Substance P and neuropeptide Y were discovered as early diagnostic biomarkers of acute myocardial infarction in Korean patients and confirmed using enzyme-linked immunosorbent assay (ELISA). We screened 12 peptides from the sera of Korean acute myocardial infarction (AMI) patients and detected 3 peptides (neuropeptide Y, substance P, and N-terminal pro-B-type natriuretic peptide) to be elevated from patients' sera by liquid chromatography mass/mass spectrometry. The elevated concentration of 3 peptides was confirmed by ELISA. The screening results revealed the substance P, neuropeptide Y, and pro-B-type natriuretic peptide (47-76) concentrations were higher in patients' sera than in healthy controls. The sensitivity and specificity of substance P for AMI diagnostic marker were 80% and 83%, respectively, and those of neuropeptide Y were 87% and 90%, respectively compared to healthy controls. These results suggest that substance P and neuropeptide Y could be used as early diagnostic biomarkers in patients with AMI.

• Child Health Nursing Research
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• v.12 no.3
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• pp.351-358
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• 2006

Purpose: This study was attempted to find out the differences of characteristics between potential substance-abuse group and healthy group of middle school students in Busan. Method: The participants were 458 middle school students in Busan. The data were collected from 1st September 2004 to 31th October 2004 using structured questionnaire, and then were analyzed by SPSS-WIN 10.0 program with frequency, percentage, mean, standard deviation and $X^2-test$. Results: 23.6% of the subjects have been smoking, and 16.2% of them have been drinking. 18.6% of the subjects was classified to potential substance-abuse group based on the cut-point suggested by Lee et al.. The characteristics of potential substance-abuse adolescents are that they have religion and siblings, that their parents have divorced, that they don't live with their parents, and that their perceived health state is bad. Conclusion: Based on the findings of this study, It is necessary to develop a substance abuse prevention education program for potential substance-abuse group.

• 한국연소학회:학술대회논문집
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• 2014.11a
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• pp.337-338
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• 2014

In this study, a method of using a capacitance sensor was investigated as a means to measure the mass fraction of a type of harmful substance. Using MEMS process, we developed a capacitance sensor and studied the real time mass fraction with harmful substance mixture liquid.

• The Journal of the Korean Society for Microbiology
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• v.6 no.1
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• pp.9-13
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• 1971

The bacterial substance inhibited the methylene blue-uptaking activity of red cells. On keeping at room temperature the methylene blue-uptaking activity of red cells sensitized with bacterial substance was more rapidly destroyed than that of normal cells. The methylene blue-uptaking activity of red cells was more inhibited by the supernatant fraction than sediment of bacterial substance.

• Plant Resources
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• v.8 no.2
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• pp.145-154
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• 2005

The antimicrobial effect of each fraction after fractionation of an ethanol extract of leaf mustard was examined in terms of nucleic acid, chloroform, ethylacetate, and butanol. The ethylacetate fraction, which showed the strongest level of antimicrobial effect among the different ethanol extract fractions of leaf mustard, was isolated and purified using silica gel column chromatography and HPLC, respectively, to obtain a single antimicrobial substance called KLM-1. The antimicrobial effect of this substance was 10 times higher than that of the ethylacetate fraction. A further study is on the way to confirm the structure of the antimicrobial substance KLM-1 through LC/Mass and NMR.