• Journal of agriculture & life science
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• v.46 no.1
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• pp.163-176
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• 2012

TTo increase productivity of a strong extracellular alkaline protease (BCAP), stable strains of Bacillus clausii I-52 carrying another copy of BCAP gene in the chromosome were developed. Integrative vector, pHPS9-fuBCAP carrying BCAP promoter, ribosome binding site, signal sequence and active protease gene was constructed and transferred into B. clausii I-52, and integration of the constructed plasmid into chromosome was identified by PCR. An investigation was carried out on BCAP production by B. clausii I-52 and transformant C5 showing the highest relative activity of alkaline protease using submerged fermentation. Maximum enzyme activity was produced when cells were grown under the submerged fermentation conditions at $37^{\circ}C$ for 48 h with an aeration rate of 1 vvm and agitation rate of 650 rpm in a optimized medium (soybean meal 2%, wheat flour 1%, sodium citrate 0.5%, $K_2HPO_4$ 0.4%, $Na_2HPO_4$ 0.1%, NaCl 0.4%, $MgSO_47H_2O$ 0.01%, $FeSO_47H_2O$ 0.05%, liquid maltose 2.5%, $Na_2CO_3$ 0.6%). A protease yield of approximately 134,670U/ml was achieved using an optimized media, which show an increase of approximately 1.6-fold compared to that of non-transformant (83,960 U/ml). When the stability of transformant C5 was examined, the integrated plasmid pHPS9-fuBCAP was detected in the transformant after cultivation for 8 days, suggesting that it maintained stably in the chromosomal DNA of transformant C5.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.31 no.2
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• pp.204-213
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• 1986

The objective of this paper was to examine the response of rice seedling emergence and early growth under the different temperature (day/night, 29/21$^{\circ}C$, 17/17$^{\circ}C$, 12/12$^{\circ}C$) and the different seeding depth (1 cm, 2 cm, 3 cm). The trial was carried out in the phytotron and field in the Crop Experiment Station, Suwon, Korea in 1985. Calcium peroxide-coated seeds were very effctive in promoting seedling emergence, seedling establishment and eary growth of rice. Coated seeds were more effective in low temperature condition (17/17$^{\circ}C$, 12/12$^{\circ}C$) than in high temperature (29/21$^{\circ}C$) at the phytotron trial. The deeper the seeding depth, the less the emergence and seedling establishment, and the available seeding depth was I cm in the direct seeding under the flooded soil. In the field trial (seeding date, May 1) the results for the emergence and seedling establishment were similar to those in the phytotron trial. Available cultivars for the direct seeding cultivation under the flooded soil were Cheonmabyeo, Namyangbyeo, Kihobyeo, Akibare, Nakdongbyeo and Dongjinbyeo in Japonica type, and Taebaegbyeo, Samgangbyeo and Gayabyeo in Indica x Jponica type. Coefficients of variation to the emergence and seedling establishment between rice cultivars were very high. Therefore, in the direct seeding cultivation under the submerged paddy, choice of rice cultivars and improved technique for direct seeding will be more necessary.

• Proceedings of the Korean Geotechical Society Conference
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• 2010.09a
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• pp.124-143
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• 2010

Cofferdam is a temporary levee or dam structure built by using sheet pile or earth materials to prevent water infiltration during construction work of bridge, dam, harbour dock, or hydraulic structures in the river. In this regard, it is required to secure cutoff ability for dry work and workability for rapid installation and removal of the temporary dam or levee structures. In this paper, case studies for design and construction of cofferdam were performed, and water diversion method was briefed with some examples of cofferdam type as well. For the case study details of design and construction were reviewed based on cofferdams under construction related to 16 submerged weirs of "The 4-river restoration project" and dam type cofferdam respectively. From the review, it was known that the method for changing the water flow is selected based on the data from geological and geo-hydraulic site investigation in order to mitigate environmental effects by making sure if the design cross-sectional area of flow and maximum working days are sufficiently guaranteed. Finally, the primary findings and main conclusion derived are summarized that determination of applicable type of cofferdam should be checked by case study and meet design requirements such as water inflow control, constructability.

• Journal of the Korean Society of Propulsion Engineers
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• v.21 no.4
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• pp.12-20
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• 2017

The ablation characteristics of graphite nozzle throat insert was analyzed for the use in solid rocket propulsion system. The propulsion system was composed of three types of conventional nozzles, such as De-Laval type, blast tube type, and submerged type. Various kinds of propellants were used in ten kinds of propulsion system that had different shapes with each other. Total forty eight tests were performed. From the results of the analysis, it was found that the ablation rate was increased for the higher average chamber pressure and the higher oxidizer mole fraction. A useful correlation for nozzle throat ablation rate was developed in terms of the chamber pressure, oxidizer mole fraction, and throat size. The calculated ablation rates from the correlation showed agreement within ${\pm}0.10mm/s$ with the experimentally determined values.

• The KSFM Journal of Fluid Machinery
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• v.20 no.1
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• pp.74-80
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• 2017

Pump sump system or pumping stations are built to draw water from a source such as river and used for irrigation, thermal power plants etc. If pump sump is improperly shaped or sized, air entraining vortices or submerged vortices may develop. This may greatly affect pump operation if vortices grow to an appreciable extent. Moreover, the noise and vibration of the pump can be increased by the remaining of vortices in the pump flow passage. Therefore, the vortices in the pump flow passage have to be reduced for a good performance of pump sump station. In this study, the effect of pump intake leaning angle and flow rate on the pump sump internal flow has been investigated. There are three cases with different leaning angle. Moreover, a pipe type with elbow also has been studied. The flow rate with three classes of air entraining vortices has been examined and investigated by decreasing the water level. The result shows that the air entraining vortices easily occurs at the pump intake with large leaning angle. Moreover, the elbow type of the pump intake easily occurs air entraining vortices at the high flow rate (or velocity) in comparison to other pump intake type.

• Journal of Navigation and Port Research
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• v.33 no.9
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• pp.645-651
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• 2009

In this study, a series of laboratory experiments were carried out to investigate the weak reflection of regular and random water waves over a train of protruded permeable shore structures. A cylindrical slit type breakwater and the alternatives are employed and compared for reflecting and transmitting capabilities of incident waves including wave forces. A series of random waves were generated by using the Bretschneider-Mitsuyasu frequency and directional spectrum. Measured spectrum of irregular waves without breakwaters is verified by comparing with those of the input waves generated. Weak reflection is occurred at the breakwater center of the peak frequency. If the row of breakwaters is fixed at three layers and the relative height of breakwater is fixed at 0.6, around 45% of incident wave energy is reflected to offshore. It is also found that the transmission of directional random waves increases as the maximum frequency parameter increases. A very good agreement is observed. Reflection coefficients of permeable submerged breakwaters are less than those of impermeable breakwaters. The upside-down L shape is recommended for a small fishery harbor mooring in terms of reflecting capability and of practical application. The final design was applied to the wharf of a small beach of Seolly, near Namhae at the southeast coast of Korea.

• Journal of Periodontal and Implant Science
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• v.37 no.4
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• pp.671-679
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• 2007

Background: $TiUnite^{TM}$ is a highly crystalline and phosphate enriched titanium oxide surface which has a unique porous surface structure. This improved implant surface enhances bone response and reduces healing period. It also assures early stability of implant. These help to increase the success of implant. The aim of this study is to evaluate the survival rate of $TiUnite^{TM}$ surfaced single implant. Materials and methods: A retrospective analysis of 89 $TiUnite^{TM}$ surfaced implants replacing a single tooth was assessed according to their dental record. The age of the patients ranged from 17 to 82 years (mean age: $45.8{\pm}14.6)$. Data were recorded regarding the survival rate of these implants. Results: Fifty-two implants (57%) were placed in the maxilla, and 37 (43%) in the mandible. Over 75% were placed in the posterior area. Of the placed implants, 67% were the wide type. while 25% were the regular type and only 8% were of the narrow type. The single implants produced an overall clinical survival rate of 96.6% over the observation period (mean 17.9 months). Among 89 implants, only 2 implants were removed and one implant was submerged. Conclusion: According to these data, $TiUnite^{TM}$ surfaced implant in a single tooth restoration showed favorable survival rate although this study was done in a short term period.

• The Journal of Korean Academy of Prosthodontics
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• v.47 no.2
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• pp.136-147
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• 2009

Statement of problem: Since the introduction of the concept of osseointegration in dental implants, high long-term success rates have been achieved. Though the use of dental implants have increased dramatically, there are few studies on domestic implants with clinical and objective long-term data. Purpose: The aim of this retrospective study was to provide long-term data on the $Implantium^{(R)}$ implant, which features a sandblasted and acid-etched surface and internal connection with microthreads. Material and methods: 106 $Implantium^{(R)}$ implants placed in 38 patients at Yonsei University Hospital were examined to determine the effect of various factors on implant success and marginal bone loss, through clinical and radiographic results during a 6 to 30 month period. Results: 1. Out of a total of 106 implants placed in 38 patients, one fixture was lost, resulting in a 99.1% cumulative survival rate. 2. Among the 96 implants which were observed throughout the study period, the survival rates were 97.0% in the maxilla and 100% in the mandible. The survival rate in the posterior regions was 98.9% and 100% in the anterior regions. 3. The mean bone loss during the first year after prosthesis placement was 0.17 mm, while the mean annual bone loss after the first year was 0.04 mm, which was statistically less than during the first year(P<.05). 4. There was no significant difference in marginal bone loss according to age during the first year(P>.05), but after the first year, the mean annual bone loss in patients above 50 years was significantly greater(P<.05) compared with patients under 50 years. 5. No significant difference in marginal bone loss was found according to the following factors: gender, jaw, location in the arch, type of implant(submerged or non-submerged), presence of bone grafts, type of prostheses, and type of opposing dentition(P<.05). Conclusion: Based on these results, the sole factor influencing marginal bone loss was age, while factors such as gender, jaw, location in the arch, type of implant, presence of bone grafts, type of prostheses and type of opposing dentition had no significant effect on bone loss. In the present study, the success rate of the $Implantium^{(R)}$ implant with a SLA surface and internal connection with microthreads was satisfactory up to a maximum 30 month period, and the marginal bone loss was in accord with the success criteria of dental implants.

• Membrane Journal
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• v.15 no.2
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• pp.114-120
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• 2005

The purpose of this study is to obtain practical information about membrane coupled $ A_2O$ system for muncipal wastewater treatment. A flat-plate microfiltration (MF) module with a pore size $0.25\;{\mu}m$ was submerged into the aeration basin and treated water was filtrated through the membrane by continuous suction with low pressure. The system was operated with synthetic wastewater to find operational parameters of internal recycle ratio and maximum MLSS showing best water quality and long-term stability. The internal recycle was defined as type 1 for aerobic to anoxic tank and type 2 for anoxic to anaerobic tank, respectively When the flux was maintained at $0.015\;m^3/m^2/hr$ (15 LMH) with 2Q type 1 internal recycle ratio, the optimal operational setting were 10 internal recycle ratio for type 2 and maximum MLSS of 11,000 mg/L among tested conditions. At this condition, removal efficiencies of BOD, CODcr, T-N and T-P showed $97.3\%,\;94.2\%,\;64.0\%,\;63.0\%$, respectively.

• Proceedings of the Korea Committee for Ocean Resources and Engineering Conference
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• 2004.05a
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• pp.257-262
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• 2004

A series of preliminary model tests are performed to find out the wave attenuating effect of the pneumatic breakwater of environment friendly type, which is a bubble screen generated by releasing compressed air from a submerged multiple parallel manifold Rising bubbles induce vertical current, which produces horizontal currents flowing away from the bubble-screen area in both directions. Near bottom, the corresponding currents flow toward the bubble screen, thus completing the circulation pattern. The surface current moving against the direction of wave propagation causes some attenuation of the waves. It becomes more effective as the relative depth (d/ L) increases (short-period waves in deep water). With the same air-discharge, the multiple parallel manifold can be more effective for the attenuation of longer waves through optimum arrangement of manifold number. installation depth, manifold gap, etc. The pneumatic breakwater will give a wide utilization as a device for protecting harbor facilities and as a simple, mobile breakwater.