• Journal of Ginseng Research
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• 제44권4호
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• pp.570-579
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• 2020

Background: Many researchers reported that the various immune activities of red ginseng are due to acid polysaccharides. But, the exact structural characteristics of the acidic polysaccharide in red ginseng have not been fully elucidated. Therefore, we isolated the acidic polysaccharide from red ginseng and characterized the structural property of the active moiety of this polysaccharide, which contributes to the immunostimulatory activity of red ginseng. Methods: A polysaccharide (RGP-AP-I) was purified from red ginseng via size-exclusion chromatography using Sephadex G-100. Immunostimulatary activity of RGP-AP-I was investigated via anti-complementory and macrophage stimulatory activity. The structure of RGP-AP-I was characterized by HPLC, sugar composition, β-glucosyl Yariv reagent and methylation analysis. Results: Peritoneal macrophages stimulated using RGP-AP-I significantly augmented the production of various cytokines such as interleukin (IL)-6, IL-12, and tumor necrosis factor (TNF)-α. The primary structure of RGP-AP-I was elucidated by assessing its sugar composition and methylation analysis. RGP-AP-I is a 96 kDa acidic polysaccharide, and comprises nine different monosaccharides, which mainly include sugars such as rhamnose (Rha, 9.5%), galacturonic acid (GalA, 18.4%), galactose (Gal, 30.4%), and arabinose (Ara, 35.0%). RGP-AP-I exhibited an considerable reaction with the β-glucosyl Yariv reagent, revealing the presence of arabino-β-3,6-galactan. Methylation analysis indicated that RGP-AP-I comprises 21 different glycosyl linkages, such as 3-, 4-, 6- and 3,6-linked Galp; 5-linked Araf; 2,4-linked Rhap; and 4-linked GalAp, which are characteristics of rhamnogalacturonan I (RG-I). Conclusion: we assumed that the immunostimulatory activity of RGP-AP-I may be due to the RG-I structure, which comprises a main chain with a repeating linkage unit, [→2)-Rhap-(1→4)-GalAp-(1→] and three groups of side chains such as (1→5)-linked arabinan, (1→4)-linked galactan, and arabino-β-3,6-galactan, which branch at the C(O)4 positions of Rha residues in the main chain of RGP-AP-I.

• 보건교육건강증진학회지
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• 제31권3호
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• pp.73-81
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• 2014

Objectives: This study examined how adolescents' cognitive stress relates to suicidal thought, thereby offering basic data about social and institutional ways to reduce adolescent suicide. Methods: In this study, data from an online survey on adolescents' health conducted by Korea Center for Disease Control(2013, 9th) was used after altering it to fit the purpose of the study. The data was analysed using the complex sampling method and structural equation model(SEM). Results: Whilst adolescents' cognitive stress had positive effects on suicidal thought, problem drinking and cognitive depression, it had a negative effect on physical activity. The structural equation model from cognitive stress, problem drinking, cognitive depression, physical activity influenced suicidal thought. Problem drinking on the other hand had an influence on cognitive depression. Conclusion: These findings suggest that future intervention programs for prevention of adolescents' suicidal thought should also address problem drinking, cognitive depression, and cognitive stress.

• 한국생활과학회지
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• 제19권1호
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• pp.63-74
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• 2010

The purpose of the study was to examine the actual conditions of caregiver-infant ratios, group-room activity areas, evaluations of infant programs and caregiver-infant interactions based on structural and process indicators which are major factors of infant care. The subjects were 20 caregivers and 91 infants from 14 infant classes of 13 day care centers in Daejeon. An actual survey was conducted on caregiver-infant ratios and group-room activity areas, and teaching-learning plans for infants and daily schedules were gathered for the evaluation of infant programs. The caregiver-infant interactions were observed every one minute for a total of 20 minutes using Lee Wan Jeong's "Evaluation Measure of Caregiver-infant Interactions"(1999). The results of this study were as follows: First, caregiver-infant ratios ranged from 2.5 to 7 infants per caregiver, resulting in the difference of the number of infants. Second, the 14 classes for one-year-old infants were arranged in three different ways; 5 classrooms with distinctive activity areas, 2 without any divided areas and 7 containing a mix of partial activity areas. Third, in teaching-learning plans for infants, there were a large number of topics related to seasonal features and experiences while the fewest were about basic life habits. Fourth, in the caregiver-infant interactions, caregivers used more positive interactions and linguistic modeling than sensitive responses to infants and social interactions.

• 한국학교ㆍ지역보건교육학회지
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• 제14권2호
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• pp.29-45
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• 2013

Objectives: The purpose of this study was to identify relationships between experiential activity, ego-resilience, sense of community, and school life adjustment of middle school students and to provide the basic data that can contribute to the activation of experiential activities. Methods: 2,351 middle school students of panel survey conducted by National Youth Policy Institute were used in the final analysis. The structural equation model(SEM) was carried out to analyze structural relationships between experiential activity and ego-resilience, sense of community, and school life adjustment of the youth. IBM SPSS Statistics 20.0 and IBM SPSS AMOS 20.0. were used for the analysis of all data. Results: Experiential activities had a statistically significant positive effect on ego-resilience(p<0.001), school life adjustment(p<0.05), and the sense of community (p<0.001). Especially experiential activities acted on ego-resiliency, a sense of community, school life adjustment as an important parameter. Sense of community also showed to be an important mediating role between ego-resiliency and school life adjustment. Conclusions: In order to maximize the effectiveness of experiential activities, it is necessary to build the community networking enough to take full advantage of community resources and develop various experiential activities to meet the needs of youth and social change.

• 통합자연과학논문집
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• 제7권1호
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• pp.45-49
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• 2014

The (c-Jun N-terminal kinase 3) JNK3 is a potential therapeutic target for various neurological disorders. Here, a three dimensional quantitative structure-activity relationship (3D-QSAR) study on phenoxypyridine as JNK3 inhibitors was performed to rationalize the structural requirements responsible for the inhibitory activity of these compounds. The comparative molecular field analysis (CoMFA) using different partial atomic charges, was employed to understand the structural factors affecting JNK3 inhibitory potency. The Gasteiger-Marsili yielded a CoMFA model with cross-validated correlation coefficient ($q^2$) of 0.54 and non-cross-validated correlation coefficient ($r^2$) of 0.93 with five components. Furthermore, contour maps suggested that bulky substitution with oxygen atom in $R^3$ position could enhance the activity considerably. The work suggests that further chemical modifications of the compounds could lead to enhanced activity and could assist in the design of novel JNK3 inhibitors.

• 성인간호학회지
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• 제20권4호
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• pp.626-640
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• 2008

Purpose: The aim of this study was to construct leisure physical activity model of middle-aged women in urban area. Methods: Data were gathered by self-report questionnaire from 211 women aged between 41 and 59 years in urban community. The data were analyzed using the SPSS/WIN 10.0 program and the model was constructed using the LISREL 8.54 program. Results: Variables that have direct effects on leisure physical activity were health state, past leisure physical activity, social support, self-efficacy, and affect. Perceived leisure state and behavioral leisure attitude also influenced leisure physical activity in an indirect way. Perceived leisure state had a direct effect on self-efficacy. Behavioral leisure attitude, past leisure physical activity, and experience of exercise effect had significantly direct effects on affect Conclusion: It will provide basic information for developing strategies of programs to enhance leisure physical activity of middle-aged women in urban area.

• 대한교통학회지
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• 제20권6호
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• pp.129-140
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• 2002

본 연구에서는 개인의 사회, 경제적 특성과 활동참여, 통행행동의 관계를 규명하기 위하여 개인의 사회 경제적 특성을 시간 변동 외생변수와, 시간 불변동 외생변수로 설정하고 개인의 활동참여와 통행행동에 관한 변수를 내생변수로 설정하여 모형을 구축하였다. 모형의 실효성을 검증하기 위하여 분석에 사용된 자료는 1991년, 1992년 미국 Puget Sound 지역의 Transportation Survey 자료를 이용하였으며, Panel 자료의 변수 간 복잡한 상호관계를 규명해 내기 위하여 Dynamic Structural Equation Model을 구축하였다. 활동참여와 통행행 동에 관한 10개의 내생변수와 개인의 사회, 경제적 특성인 10개의 외생변수를 사용하여 모형추정을 한 결과, 개인의 생계활동에 의해 유지활동과 여가활동은 시간적 제약을 받고, 여가활동은 유지활동에 제약을 받게 된다는 것을 알게 되었으며, 개인의 사회경제지표에 의해 영향을 받는 것으로 나타났다. 또한, 개인의 활동 패턴은 과거 연도의 각 활동 지속시간과 통행패턴에 영향을 받는 것으로 나타나 Panel 자료 분석의 필요성이 부각되었다. 본 연구의 결과로부터 보다 충실한 활동기반모형의 구축을 위해서는 Panel 자료의 구축의 필요성이 더욱 요구된다.

• Genomics & Informatics
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• 제10권2호
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• pp.128-132
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• 2012

To learn the differences between the structure-activity relationship and molecular vibration-activity relationship in the ligand-receptor interaction of the histamine receptor, 47 ligands of the histamine receptor were analyzed by structural similarity and molecular vibrational frequency patterns. The radial tree that was produced by clustering analysis of molecular vibrational frequency patterns shows its potential for the functional classification of histamine receptor ligands.

• 한국응용약물학회:학술대회논문집
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• 한국응용약물학회 1998년도 Proceedings of UNESCO-internetwork Cooperative Regional Seminar and Workshop on Bioassay Guided Isolation of Bioactive Substances from Natural Products and Microbial Products
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• pp.52-55
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• 1998

During a survey of new antitumor substances from higher plants, we have found that the crude extract of Simaba cedron Planchon (Simaroubaceae) showed cytotoxic activity (IC$\sub$50/ 0.7 $\mu\textrm{g}$/mL) against P388 leukemia cells. Activity-guided chromatographic purification using P388 cells led to the isolation of five novel quassinoids, cedronolactones A-E (1-5) and nine known quassinoids, simalikalactone D (6), chaparrinon (7), chaparrin (8), glaucarubolone (9), glaucarubol (10), samaderine Z (11), guanepolide (12), ailanquassin A (13), and polyandrol (14). In this seminar, the structural elucidation of 1-5 and the cytotoxic activity of the isolated compounds are discussed.

• 대한토목학회논문집
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• 제28권2D호
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• pp.171-179
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• 2008

본 연구는 토요일에 생계활동에 참여하는 출근자와 생계활동에 참여하지 않고 여가활동만 참여하는 휴무자를 대상으로 활동참여 및 통행행태간의 관계를 분석하였다. 분석결과 각각의 활동참여는 통행을 유발시켜 통행시간이 발생되며, 통행시간이 모여 통행량이 발생하는 3단계의 인과구조를 이루고 있음을 확인하였다. 출근자의 경우 생계활동시간과 여가활동시간의 관계를 보면, 부(-)의 관계를 가짐에 따라 생계활동시간이 길어질 때 여가활동시간이 짧아짐을 알 수 있다. 이는 시간적인 제약(time budget)으로 인해 생계활동시간이 증가할 때 여가활동에 투입되는 시간이 짧아짐에 따라 나타나는 현상이다. 생계활동시간과 통행시간, 생계활동시간과 통행수 관계 역시 부(-)의 관계를 가짐에 따라 생계활동시간이 증가할 때 통행시간과 통행수가 감소한다. 여가활동시간과 통행시간 및 통행수의 관계를 보면 출근자 및 휴무자 모두 양(+)의 관계를 보인다. 따라서 여가활동시간이 길어진다는 것은 많은 여가활동에 참여하는 것으로 이에 따라 통행시간 및 통행수가 증가한다.