• 산업기술연구
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• 제24권B호
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• pp.139-142
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• 2004

To identify conserved structural or functional subunit(s) in the CP1 (editing) domains of class Ia tRNA synthetases, five available structures were compared and analyzed. Through sequence alignments of the CP1 domains, three conserved regions were found near the amino acid binding site in the editing domain. Structural overlapping of the three subunits clearly showed that there exist three common structural subunits in all of the five editing RS structures. The new alignment suggests a translocation movement of the CP1 domain caused by the binding with tRNA. Based on the experimental and modeling results, it is proposed that subunits 1 and 3 accommodate the incoming amino acid binding, while subunit 2 contributes to the interactions with the adenosine ring of the A76 to stabilize the overall tRNA binding.. Since these subunits are critical for the editing reaction, we expect that these key structures should be conserved through all class Ia editing RSs.

• Bulletin of the Korean Chemical Society
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• 제28권2호
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• pp.207-210
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• 2007

To identify structural or functional common subunit(s) in the CP1 (editing) domains of class Ia tRNA synthetases, five available structures were compared and analyzed. Through the sequence alignments and structural overlapping of the CP1 domains, three conserved regions were identified near the amino acid binding site in the editing domain. Structural overlapping of the three subunits clearly showed the existence of three common structural subunits in all of the five editing RS structures. Based on the established experimental results and our modeling results, it is proposed that subunits 1 and 3 accommodate the incoming amino acid binding, while subunit 2 contributes to the interactions with the adenosine ring of the A76 to stabilize the overall tRNA binding. Since these subunits are critical for the editing reaction, we expect that these key structures should be conserved through the most class Ia editing RSs.

• BMB Reports
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• 제57권1호
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• pp.12-18
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• 2024

Due to the development of CRISPR technology, the era of effective editing of target genes has arrived. However, the off-target problem that occurs when recognizing target DNA due to the inherent nature of CRISPR components remains the biggest task to be overcome in the future. In this review, the principle of inducing such unintended off-target editing is analyzed from the structural aspect of CRISPR, and the methodology that has been developed to reduce off-target editing until now is summarized.

• BMB Reports
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• 제49권8호
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• pp.431-436
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• 2016

Human papillomavirus (HPV) is the major cause of cervical cancer, a deadly threat to millions of females. The early oncogene product (E7) of the high-risk HPV16 is the primary agent associated with HPV-related cervical cancers. In order to understand how E7 contributes to the transforming activity, we investigated the structural features of the flexible N-terminal region (46 residues) of E7 by carrying out N-15 heteronuclear NMR experiments and replica exchange molecular dynamics simulations. Several NMR parameters as well as simulation ensemble structures indicate that this intrinsically disordered region of E7 contains two transient (10-20% populated) helical pre-structured motifs that overlap with important target binding moieties such as an E2F-mimic motif and a pRb-binding LXCXE segment. Presence of such target-binding motifs in HPV16 E7 provides a reasonable explanation for its promiscuous target-binding behavior associated with its transforming activity.

• 한국측량학회지
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• 제39권6호
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• pp.363-369
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• 2021

정밀도로지도는 자율주행차의 기본 인프라로 활용되어 최신 도로정보가 신속하게 반영되어야 한다. 하지만 현재 정밀도로지도 공정 중 객체 도화 및 구조화 편집과정이 수작업으로 이루어지며 주요 구축 대상인 도로 노면선 표시의 레이어를 생성하는데 가장 오랜 시간이 소요된다. 이에 본 연구에서는 선행 연구에서 기학습된 포인트넷(PointNet) 모델을 통해 색상 유형(백색, 청색, 황색)이 예측된 도로 노면선 표시의 포인트 클라우드를 입력 데이터로 활용하였고, 이를 기반으로 본 연구에서는 도로 노면선 표시 레이어의 도화 및 구조화 편집을 자동화하는 방법론을 제안하였다. 제안한 방법론을 통해 구축한 3차원 벡터 데이터의 활용성을 검증하기 위해 정밀도로지도 품질검사 기준에 따라 정확도를 분석하였다. 벡터 데이터의 위치정확도 검사에서 수평 오차와 수직 오차에 대한 평균제곱근오차(RMSE: Root Mean Square Error)는 0.1m 이내로 나타나 적합성을 검증하였으며, 구조화 편집 정확도 검사에서 선표시 유형과 선규제 유형의 구조화 정확도가 모두 88.235%로 나타나 활용성을 검증하였다. 따라서, 본 연구에서 제안한 방법론으로 정밀도로지도를 위한 도로 노면선 표시의 벡터 데이터를 효율적으로 구축할 수 있는 것을 알 수 있었다.

• 한국정보통신학회:학술대회논문집
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• 한국해양정보통신학회 2002년도 추계종합학술대회
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• pp.363-367
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• 2002

컴퓨터를 이용한 전자문서처리에 이용되는 기존의 문서편집 시스템에서는 비구조적인 문서구조를 다루게 됨으로써 시스템 내에서 사용되는 수학식의 표현은 이미지나 텍스트 등의 비구조적인 방법에 의해 표현되거나 처리된다. 이렇게 사용된 수학식은 문서의 가독성과 재사용성 그리고 문서의 처리와 교환에 상대적인 불편을 초래하게 된다. 그러므로 이러한 단점을 극복하고 효율적으로 구조적인 문서상에 MathML(Mathematical Markup Language) 수식 구조를 적용시킬 수 있는 문서편집 시스템이 요구된다. 이에 본 논문에서는 MathML을 기반으로 한 수학식의 표현을 구조적으로 처리할 수 있는 MathML 수식 편집이 가능한 XML(eXtensible Markup Language) 기반의 구조적 문서생성을 위한 문서 편집 시스템을 설계 및 구현하였다.

• BMB Reports
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• 제56권2호
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• pp.102-107
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• 2023

Genome editing using CRISPR-associated technology is widely used to modify the genomes rapidly and efficiently on specific DNA double-strand breaks (DSBs) induced by Cas9 endonuclease. However, despite swift advance in Cas9 engineering, structural basis of Cas9-recognition and cleavage complex remains unclear. Proper assembly of this complex correlates to effective Cas9 activity, leading to high efficacy of genome editing events. Here, we develop a CRISPR/Cas9-RAD51 plasmid constitutively expressing RAD51, which can bind to single-stranded DNA for DSB repair. We show that the efficiency of CRISPR-mediated genome editing can be significantly improved by expressing RAD51, responsible for DSB repair via homologous recombination (HR), in both gene knock-out and knock-in processes. In cells with CRISPR/Cas9-RAD51 plasmid, expression of the target genes (cohesin SMC3 and GAPDH) was reduced by more than 1.9-fold compared to the CRISPR/Cas9 plasmid for knock-out of genes. Furthermore, CRISPR/Cas9-RAD51 enhanced the knock-in efficiency of DsRed donor DNA. Thus, the CRISPR/Cas9-RAD51 system is useful for applications requiring precise and efficient genome edits not accessible to HR-deficient cell genome editing and for developing CRISPR/Cas9-mediated knockout technology.

• 대한공간정보학회지
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• 제13권2호
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• pp.71-77
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• 2005

GIS의 기초가 되는 수치지도의 활용은 빠르게 증가하고 있는 추세이다. 수치지도의 제작 공정에 있어서 많은 발전이 있어 왔지만 지리조사와 구조화편집 공전에는 여전히 지도 출력물에 의존하는 기존의 방법을 사용하고 있었다. 이에 본 연구는 이러한 공정에 현장측량시스템의 활용하는 방법을 제안하였다. 제안된 방법을 수치지도 Ver 2.0 제작에 적용하여 기존의 방법에 의한 결과와 비교하였다. 비교 분석을 통해 제안된 방법은 수치지도 제작 과정에 필요한 소요 인원 및 처리 단계가 줄임을 통해 전체 비용의 5% 정도의 절감할 수 있다는 것을 보였다.

• Molecules and Cells
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• 제41권10호
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• pp.889-899
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• 2018

Intrinsically disordered proteins (IDPs) are highly unorthodox proteins that do not form three-dimensional structures under physiological conditions. The discovery of IDPs has destroyed the classical structure-function paradigm in protein science, 3-D structure = function, because IDPs even without well-folded 3-D structures are still capable of performing important biological functions and furthermore are associated with fatal diseases such as cancers, neurodegenerative diseases and viral pandemics. Pre-structured motifs (PreSMos) refer to transient local secondary structural elements present in the target-unbound state of IDPs. During the last two decades PreSMos have been steadily acknowledged as the critical determinants for target binding in dozens of IDPs. To date, the PreSMo concept provides the most convincing structural rationale explaining the IDP-target binding behavior at an atomic resolution. Here we present a brief developmental history of PreSMos and describe their common characteristics. We also provide a list of newly discovered PreSMos along with their functional relevance.

• 한국의류학회지
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• 제45권3호
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• pp.510-524
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• 2021

Selfie practices on social media can result in negative body image for men. The current study investigated the dual body image pathway model for the relationship between selfie practices on social media and body satisfaction, with internalization as a mediator. Structural equation modeling analyses supported our research model when studying 446 young Korean male Instagram users. The results indicated that selfie editing behavior, but not selfie browsing behavior, significantly predicted an increased internalization of a body ideal. The positive associations between internalization, muscularity dissatisfaction, and body fat dissatisfaction were confirmed. Additionally, the indirect effects of selfie editing behavior on muscularity and body fat dissatisfaction due to internalization were significant. The study confirmed the detrimental effects of selfie behavior on body satisfaction for male social media users. The results provided valuable information that selfie editing may be a risky behavior since it can result in developing muscularity and body fat dissatisfaction. Theoretical contributions and practical implications were discussed. Future research should address the age and cultural differences that may elucidate the impacts of selfie practices on men's body image concerns.