• Journal of Industrial Technology
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• v.24 no.B
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• pp.139-142
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• 2004

To identify conserved structural or functional subunit(s) in the CP1 (editing) domains of class Ia tRNA synthetases, five available structures were compared and analyzed. Through sequence alignments of the CP1 domains, three conserved regions were found near the amino acid binding site in the editing domain. Structural overlapping of the three subunits clearly showed that there exist three common structural subunits in all of the five editing RS structures. The new alignment suggests a translocation movement of the CP1 domain caused by the binding with tRNA. Based on the experimental and modeling results, it is proposed that subunits 1 and 3 accommodate the incoming amino acid binding, while subunit 2 contributes to the interactions with the adenosine ring of the A76 to stabilize the overall tRNA binding.. Since these subunits are critical for the editing reaction, we expect that these key structures should be conserved through all class Ia editing RSs.

• Bulletin of the Korean Chemical Society
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• v.28 no.2
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• pp.207-210
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• 2007

To identify structural or functional common subunit(s) in the CP1 (editing) domains of class Ia tRNA synthetases, five available structures were compared and analyzed. Through the sequence alignments and structural overlapping of the CP1 domains, three conserved regions were identified near the amino acid binding site in the editing domain. Structural overlapping of the three subunits clearly showed the existence of three common structural subunits in all of the five editing RS structures. Based on the established experimental results and our modeling results, it is proposed that subunits 1 and 3 accommodate the incoming amino acid binding, while subunit 2 contributes to the interactions with the adenosine ring of the A76 to stabilize the overall tRNA binding. Since these subunits are critical for the editing reaction, we expect that these key structures should be conserved through the most class Ia editing RSs.

• BMB Reports
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• v.57 no.1
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• pp.12-18
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• 2024

Due to the development of CRISPR technology, the era of effective editing of target genes has arrived. However, the off-target problem that occurs when recognizing target DNA due to the inherent nature of CRISPR components remains the biggest task to be overcome in the future. In this review, the principle of inducing such unintended off-target editing is analyzed from the structural aspect of CRISPR, and the methodology that has been developed to reduce off-target editing until now is summarized.

• BMB Reports
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• v.49 no.8
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• pp.431-436
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• 2016

Human papillomavirus (HPV) is the major cause of cervical cancer, a deadly threat to millions of females. The early oncogene product (E7) of the high-risk HPV16 is the primary agent associated with HPV-related cervical cancers. In order to understand how E7 contributes to the transforming activity, we investigated the structural features of the flexible N-terminal region (46 residues) of E7 by carrying out N-15 heteronuclear NMR experiments and replica exchange molecular dynamics simulations. Several NMR parameters as well as simulation ensemble structures indicate that this intrinsically disordered region of E7 contains two transient (10-20% populated) helical pre-structured motifs that overlap with important target binding moieties such as an E2F-mimic motif and a pRb-binding LXCXE segment. Presence of such target-binding motifs in HPV16 E7 provides a reasonable explanation for its promiscuous target-binding behavior associated with its transforming activity.

• Journal of the Korean Society of Surveying, Geodesy, Photogrammetry and Cartography
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• v.39 no.6
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• pp.363-369
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• 2021

High-definition road maps are used as the basic infrastructure for autonomous vehicles, so the latest road information must be quickly reflected. However, the current drawing and structural editing process of high-definition road maps are manually performed. In addition, it takes the longest time to generate road lanes, which are the main construction targets. In this study, the point cloud of the road lane markings, in which color types(white, blue, and yellow) were predicted through the PointNet model pre-trained in previous studies, were used as input data. Based on the point cloud, this study proposed a methodology for automatically drawing and structural editing of the layer of road lane markings. To verify the usability of the 3D vector data constructed through the proposed methodology, the accuracy was analyzed according to the quality inspection criteria of high-definition road maps. In the positional accuracy test of the vector data, the RMSE (Root Mean Square Error) for horizontal and vertical errors were within 0.1m to verify suitability. In the structural editing accuracy test of the vector data, the structural editing accuracy of the road lane markings type and kind were 88.235%, respectively, and the usability was verified. Therefore, it was found that the methodology proposed in this study can efficiently construct vector data of road lanes for high-definition road maps.

• Proceedings of the Korean Institute of Information and Commucation Sciences Conference
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• 2002.11a
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• pp.363-367
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• 2002

Represent of mathematical formula used within system handling document that is nonstructural in existent document editing system that is used in electron document processing that use computer is represented or processed by method that is nonstructural of image or text or etc. Such mathematical formular causes relative inconvenience to readablility and reusability of document and processing and exchange of document. Therefore, document editing system is required that can overcome such nonadvantage and apply MathML mathematical formula structure on efficiently structural document. Therefore, designed and implemented that document editing system for structural document creation of XML base that can mathematical formular editing of MathML base in this paper.

• BMB Reports
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• v.56 no.2
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• pp.102-107
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• 2023

Genome editing using CRISPR-associated technology is widely used to modify the genomes rapidly and efficiently on specific DNA double-strand breaks (DSBs) induced by Cas9 endonuclease. However, despite swift advance in Cas9 engineering, structural basis of Cas9-recognition and cleavage complex remains unclear. Proper assembly of this complex correlates to effective Cas9 activity, leading to high efficacy of genome editing events. Here, we develop a CRISPR/Cas9-RAD51 plasmid constitutively expressing RAD51, which can bind to single-stranded DNA for DSB repair. We show that the efficiency of CRISPR-mediated genome editing can be significantly improved by expressing RAD51, responsible for DSB repair via homologous recombination (HR), in both gene knock-out and knock-in processes. In cells with CRISPR/Cas9-RAD51 plasmid, expression of the target genes (cohesin SMC3 and GAPDH) was reduced by more than 1.9-fold compared to the CRISPR/Cas9 plasmid for knock-out of genes. Furthermore, CRISPR/Cas9-RAD51 enhanced the knock-in efficiency of DsRed donor DNA. Thus, the CRISPR/Cas9-RAD51 system is useful for applications requiring precise and efficient genome edits not accessible to HR-deficient cell genome editing and for developing CRISPR/Cas9-mediated knockout technology.

• Journal of Korean Society for Geospatial Information Science
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• v.13 no.2 s.32
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• pp.71-77
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• 2005

Digital maps that art the primitive components for GIS has been increasingly used for various applications. Although their production processes has been noticeably improved, particularly, the geographic survey and structural editing processes still depends on the traditional process depending on maps printed on papers. This study thus proposes a new efficient method employing a computerized filed survey system into these processes. We applied this method to producing a digital map ver 2.0 and compare the results nth those based on the existing method. From this comparison analysis, it is shown that the proposed method saves about 5% from the overall cost by reducing the man power and processing steps required for digital map production.

• Molecules and Cells
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• v.41 no.10
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• pp.889-899
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• 2018

Intrinsically disordered proteins (IDPs) are highly unorthodox proteins that do not form three-dimensional structures under physiological conditions. The discovery of IDPs has destroyed the classical structure-function paradigm in protein science, 3-D structure = function, because IDPs even without well-folded 3-D structures are still capable of performing important biological functions and furthermore are associated with fatal diseases such as cancers, neurodegenerative diseases and viral pandemics. Pre-structured motifs (PreSMos) refer to transient local secondary structural elements present in the target-unbound state of IDPs. During the last two decades PreSMos have been steadily acknowledged as the critical determinants for target binding in dozens of IDPs. To date, the PreSMo concept provides the most convincing structural rationale explaining the IDP-target binding behavior at an atomic resolution. Here we present a brief developmental history of PreSMos and describe their common characteristics. We also provide a list of newly discovered PreSMos along with their functional relevance.

• Journal of the Korean Society of Clothing and Textiles
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• v.45 no.3
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• pp.510-524
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• 2021

Selfie practices on social media can result in negative body image for men. The current study investigated the dual body image pathway model for the relationship between selfie practices on social media and body satisfaction, with internalization as a mediator. Structural equation modeling analyses supported our research model when studying 446 young Korean male Instagram users. The results indicated that selfie editing behavior, but not selfie browsing behavior, significantly predicted an increased internalization of a body ideal. The positive associations between internalization, muscularity dissatisfaction, and body fat dissatisfaction were confirmed. Additionally, the indirect effects of selfie editing behavior on muscularity and body fat dissatisfaction due to internalization were significant. The study confirmed the detrimental effects of selfie behavior on body satisfaction for male social media users. The results provided valuable information that selfie editing may be a risky behavior since it can result in developing muscularity and body fat dissatisfaction. Theoretical contributions and practical implications were discussed. Future research should address the age and cultural differences that may elucidate the impacts of selfie practices on men's body image concerns.