• 원예과학기술지
• /
• 제28권5호
• /
• pp.891-894
• /
• 2010

본 연구는 원예치료가 치매노인의 스트레스와 혈중 코티졸에 미치는 영향에 대해서 알아보기 위하여 노인시설에서 보호를 받고 있는 치매노인 중에서 약물을 복용하지 않으며 혈액검사에 동의한 자를 대상으로 하였다. 원예치료 프로그램은 치매노인의 스트레스 감소를 궁극적 목표로 대상자들이 선호하는 꽃 장식만을 실시한 결과 주관적 스트레스 측정결과 대조군은 12.88점에서 17.88점으로 스트레스 수치가 높아진 반면에 실험군은 원예치료 실시 전 13.88점에서 실시 후 6.38점으로 유의하게(p = 0.007) 낮아졌다. 스트레스의 생리적인 변화로 혈중 코티졸 호르몬을 측정한 결과 원예치료 실시 전 $11.33{\mu}g{\cdot}L^{-1}$에서 실시 후에 $9.85{\mu}g{\cdot}L^{-1}$로 혈중 코티졸 호르몬의 수치가 유의하게(p = 0.037) 낮아지는 것을 볼 수 있었다. 이와 같이 원예치료는 혈중 코티졸 호르몬 수치를 낮추어 주어 스트레스 감소에 효과적인 것을 알 수 있었다.

• Journal of Ginseng Research
• /
• 제43권1호
• /
• pp.125-134
• /
• 2019

Background: Excessive stress causes varied physiological and psychological disorders including male reproductive problems. Here, we attempted to investigate the protective effects of Korean Red Ginseng (Panax ginseng Meyer; KRG) against sub-acute immobilization stress-induced testicular damage in experimental rats. Methods: Male rats (age, 4 wk; weight, 60-70 g) were divided into four groups (n = 8 in each group): normal control group, immobilization control group, immobilization group treated with 100 mg/kg of KRG daily, and immobilization group treated with 200 mg/kg of KRG daily. Normal control and immobilization control groups received vehicle only. KRG (100 mg/kg and 200 mg/kg) was mixed in the standard diet powder and fed daily for 6 mo. Parameters such as organ weight, blood chemistry, sperm kinematic values, and expression levels of testicular-related molecules were measured using commercially available kits, Western blotting, and reverse transcription polymerase chain reaction. Results: Data revealed that KRG restored the altered testis and epididymis weight in immobilization stress-induced rats significantly (p < 0.05). Further, KRG ameliorated the altered blood chemistry and sperm kinematic values when compared with the immobilization control group and attenuated the altered expression levels of spermatogenesis-related proteins (nectin-2, cAMP responsive element binding protein 1, and inhibin-${\alpha}$), sex hormone receptors (androgen receptor, luteinizing hormone receptor, and follicle-stimulating hormone receptor), and antioxidant-related enzymes (glutathione S-transferase m5, peroxiredoxin-4, and glutathione peroxidase 4) significantly in the testes of immobilization stress-induced rats. Conclusion: KRG protected immobilization stress-induced testicular damage and fertility factors in rats, thereby indicating its potential in the treatment of stress-related male sterility.

• IMMUNE NETWORK
• /
• 제5권4호
• /
• pp.247-251
• /
• 2005

Background: Natural killer (NK) cells are CD3 (-) CD14 (-) CD56 (+) lymphocytes. They play an important role in the body's innate immune response. They can induce spontaneous killing of cancer cells or virus-infected cells via the Fas/Fas ligand or the granzyme/perforin systems. The corticotropin-releasing hormone (CRH) is an important regulator for the body's stress response. It promotes proliferation and migration of various cancer cells through the CRH type 1 receptor under stress, and also inhibits NK or T cell activity. However, the relationship of CRH and NK cell migration to the target has not been confirmed. Herein, we study the effect of CRH on NK cell migration. Methods: We used the human NK cell line, NK-92MI, and tested the expression of CRH receptor type 1 on NK-92MI by RT-PCR. This was to examine the effect of CRH on tumor and NK cell migration, thus NK cells (NK-92MI) were incubated with or without CRH and then each CRH treated cell's migration ability compared to that of the CRH untreated group. Results: We confirmed that CRH receptor type 1 is expressed in NK-92MI. CRH can decrease NK cell migration in a time-/dose-dependent manner. Conclusion: These data suggest CRH can inhibit NK cell migration to target cells.

• Clinical and Experimental Reproductive Medicine
• /
• 제45권3호
• /
• pp.116-121
• /
• 2018

Objective: The aim of this study was to compare serum fetuin-A levels and oxidative stress markers, as indicators of insulin resistance, in women with polycystic ovary syndrome (PCOS) and in healthy controls. Methods: This prospective case-control study included 46 patients with PCOS and 48 age- and body mass index-matched control women. Levels of serum hormones, fetuin-A, and oxidative stress markers were measured in blood samples taken during the early follicular period from each participant. Results: Follicle-stimulating hormone (FSH), luteinising hormone (LH), total testosterone levels, and the LH/FSH ratio were found to be significantly higher in women with PCOS than in controls. Serum total antioxidant status, total oxidant status, and oxidative stress index parameters all indicated significantly higher levels of oxidative stress in PCOS patients than in controls. Serum fetuin-A levels, which were analyzed as an indicator of insulin resistance, were higher in the PCOS group than in the control group ($210.26{\pm}65.06{\mu}g/mL$ and $182.68{\pm}51.20{\mu}g/mL$, respectively; p= 0.024). Conclusion: The data obtained from the present study suggest that higher levels of both serum fetuin-A and oxidative stress markers might be related with PCOS.

• 한국수정란이식학회지
• /
• 제26권3호
• /
• pp.165-169
• /
• 2011

The present study investigated the physiological evaluation of cloned mini-pigs in a transportable isolator. Transportable isolator was designed and manufactured by our research team for transporting gnotobiotic pig. Until now, no previous reports are available regarding the physiological activities and harmful effects when pigs were transported in this isolator. Five cloned mini-pigs of 1~2 year (s) old female with a body weight between 80~90 kg were used. The effects of transportable isolator on stress-related hormone, adrenocorticotrophic hormone (ACTH) and cortisol levels, and heart rate were evaluated. In addition, it was also examined the effects of transportable isolator on blood chemistry factors (alanine aminotransferase: ALT, aspartate aminotransferase: AST, blood urea nitrogen: BUN, glucose, and creatinine). Blood was sampled just before the beginning of transport (T0), at the end of transport (30min after the transport; T1), and 30 min after the end of transport (T2). At the same time, heart rate was also evaluated. As a result, heart rate had no significant (p>0.05) differences at the various-time points of study (T0, T1, T2). However, heart rate was slightly higher than normal range in T1 and T2. The ACTH level was higher than normal range. Whereas, the cortisol level was lower than normal range. There were no statistical significant differences both ACTH and cortisol level between different time groups. Also, there were no significant differences in blood chemistry factors. Therefore, our present study shows that transportable isolator has no harmful effect on stress and physiological condition in cloned mini-pigs.

• Journal of Acupuncture Research
• /
• 제15권2호
• /
• pp.157-171
• /
• 1998

In order to study the effect of direct moxibustion on depressed immune response and thyroid hormone in rats exposed to cold stress, Sprague-Dawley male rats were put in the horizontal refrigerator by $-10^{\circ}C$ for 14 days and thereafter $-18^{\circ}C$ for 11 days(control group). Sample I group was treated by daily direct moxibustion to bilateral Shinsu(BL23) from the 22nd day to the 25th day for 4 days under the same condition with the control group. Sample II group was treated by daily direct moxibustion to bilateral non-acupoints near the root of the tail from the 22nd day to the 25th day for 4 days under the same condition with the control group. And RBC, WBC, CD4+ T cell count, T3, T4 and TSH were measured. The results were as follows; 1. RBC increased with statistical significance in the sample I and sample II groups compared with the control group. 2. WBC increased with statistical significance in the sample I group, but there was not any significance in the sample II group compared with the control group. 3. Lymphocyte increased with statistical significance both in the sample I and sample II groups compared with the control group. 4. CD4+ T cell count increased with statistical significance both in the sample I and sample II groups compared with the control group. 5. T3, T4 and TSH increased respectively with statistical significance in the sample I and sample II groups compared with the control group.

• Clinical and Experimental Reproductive Medicine
• /
• 제49권1호
• /
• pp.49-56
• /
• 2022

Objective: Oxidative stress and sperm DNA fragmentation (SDF) have been linked to idiopathic male infertility (IMI). Various antioxidants have been tried to improve semen parameters and fertility potential in IMI patients, but with inconsistent results. The study aimed to compare the effects of coenzyme Q10 (CoQ10) and Centrum multivitamins on semen parameters, seminal antioxidant capacity, and SDF in infertile men with idiopathic oligoasthenospermia (OA). Methods: This prospective controlled clinical study involved 130 patients with idiopathic OA and 58 fertile controls. The patients were divided randomly into two groups: the first group received CoQ10 (200 mg/day orally) and the second group received Centrum multivitamins (1 tablet/day) for 3 months. Semen parameters, CoQ10 levels, reactive oxygen species (ROS), total antioxidant capacity (TAC), catalase, SDF, and serum hormone levels (follicle-stimulating hormone, luteinizing hormone, testosterone, and prolactin) were compared at baseline and after 3 months. Results: Both CoQ10 and Centrum improved sperm concentration and motility, but the improvement was greater with Centrum therapy (p<0.05). Similarly, both therapies improved antioxidant capacity, but TAC and catalase improvement was greater (p<0.01 and p<0.001 respectively) with CoQ10, whereas ROS (p<0.01) and SDF (p<0.001) improvements were greater with Centrum administration. Centrum therapy was associated with reduced serum testosterone (p<0.05). Conclusion: In conclusion, both CoQ10 and Centrum were effective in improving semen parameters, antioxidant capacity, and SDF, but the improvement was greater with Centrum than with CoQ10. Therefore, Centrum-as a source of combined antioxidants-may provide more effective results than individual antioxidants such as CoQ10 in the treatment of infertile men with idiopathic OA.

• 부모자녀건강학회지
• /
• 제2권
• /
• pp.30-52
• /
• 1999

The purpose of this study is to clarify that neonatal massage is a nursing intervention which is desirable to LBW by making nurses provide such massage service to LBW hospitalized at NICU and confirming effects of the service on weight gain, vital signs and stress hormones(cortisol, norepinephrine, epinephrine). The data were collected from Dec. 14, 1998 to Oct. 4, 1999. The subjects were 28 LBW infants. The data were analyzed with the SAS program with T-test, Chi-square test and ANOVA. 1. The Experimental group that received neonatal massage showed a higher daily gain of weight than the comparative group. Weight gain for 10 days and weight gain of seven days after the massage were also higher in the former group than in the latter, indicating that there are statistically significant differences between the two groups.

• Journal of Plant Biotechnology
• /
• 제44권1호
• /
• pp.61-68
• /
• 2017

GASA는 GA에 의해 조절되는 식물 유전자로서, 여러 식물에 보존되어 있고 다양한 조직에서 식물의 생장과 발달 및 스트레스 반응에 관여하는 것으로 알려져 있다. 본 연구에서는 GASA 유전자를 현사시나무(Populus alba ${\times}$ P. glandulosa)에서 분리하여 이를 PagGASA라 명명하고, 유전자의 구조와 발현특성을 조사하였다. PagGASA 유전자는 95개의 아미노산으로 구성된 단백질을 암호화하며, 아미노 말단에 시그널 펩티드 영역과 카르복시 말단에 12개 시스테인 잔기가 보존되어 있다. PagGASA는 현사시나무의 염색체에 1 ~ 2 copy 존재하며, 꽃과 뿌리에서 높게 발현하였다. 또한 PagGASA는 GA 뿐 아니라 ABA와 JA, SA와 같은 스트레스 관련 식물 호르몬의 처리에 의해서 발현이 증가하는 것으로 나타났다. 현사시나무에 형질전환하여 PagGASA를 과발현시킨 결과 건조 내성에 효과가 있음을 확인하였다. 따라서 PagGASA는 스트레스 관련 식물 호르몬 신호전달과 연결되어 건조 스트레스 방어기작에서 중요한 역할을 할 것으로 생각된다.

• 한국가축번식학회지
• /
• 제24권3호
• /
• pp.249-254
• /
• 2000

본 연구에서는 half-life가 긴 DHEAS를 스트레스 관련 호르몬으로 보고 다음과 같은 실험을 실시하였다. 실험 1) 매일 30분씩 3주간 장기 고온스트레스 부과 후 4일간의 휴식을 준 생쥐에서의 DHEAS 변화를 관찰하여 이를 cortisol과 비교하였다. 그 결과 cortisol의 경우 장기 고온스트레스 부과 후 즉시 혈액을 채취한 그룹(LW)과 4일간의 휴식 후 혈액을 채취한 그룹(LR) 모두 대조군(C)에 비해 유의적 차이를 나타내지 않았다. 이에 반해 DHEAS는 장기 고온스트레스 부과 후 즉시 혈액을 채취한 그룹(LW)에서 대조군에 비해 유의적인 감소(p<0.05)를 보였으며, 4일간의 휴식(LR)을 준 후에도 여전히 유의적인 차이를 유지하였다(p<0.05). 실험 2) 고온스트레스를 매일 30분씩 1주간, 2주간, 3주간 부과한 그룹에서의 휴식 기간별 혈청 DHEAS농도 차이를 측정하였다. 그 결과 스트레스 기간이 길어질수록 혈청 DHEAS농도가 감소되는 경향을 나타내었으며, 10일간의 휴식을 주었음에도 2주간, 3주간 부과한 그룹에서 여전히 대조군에 비해 낮은 농도를 유지하였다(p<0.05). 이러한 결과로 보아 cortisol을 이용한 기존방식은 스트레스가 사라진 후의 상태를 파악하는데 어려움이 있었던데 반해, DHEAS는 변화된 상태를 지속적으로 유지함으로써 스트레스가 사라진 후의 상태를 파악하는데 적합한 호르몬으로 판단된다.