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http://dx.doi.org/10.5010/JPB.2017.44.1.061

Characterization of Gibberellic Acid-Stimulated Arabidopsis (GASA) gene to drought stress response in Poplar (Populus alba × P. glandulosa)  

Choi, Hyunmo (Forest Biotechnology Division, National Institute of Forest Science)
Bae, Eun-Kyung (Forest Biotechnology Division, National Institute of Forest Science)
Choi, Young-Im (Forest Biotechnology Division, National Institute of Forest Science)
Yoon, Seo-Kyung (Forest Biotechnology Division, National Institute of Forest Science)
Lee, Hyoshin (Forest Biotechnology Division, National Institute of Forest Science)
Publication Information
Journal of Plant Biotechnology / v.44, no.1, 2017 , pp. 61-68 More about this Journal
Abstract
Gibberellic Acid-Stimulated Arabidopsis (GASA) genes are involved in plant hormone signaling, cell division and elongation, as well as in responses to stress conditions in plants. In this study, we isolated a GASA gene from hybrid poplar (Populus alba ${\times}$ P. glandulosa) and analyzed its physiological phenotype and molecular functions in poplar. PagGASA cDNA encodes a putative protein composed of 95 amino acids containing an N-terminal signal peptide and a conservative cysteine-rich C-terminal domain. Southern blot analysis revealed that one or two copies of the PagGASA are present in the poplar genome. The PagGASA transcripts were highly detected in flowers and roots. Moreover, the expression of PagGASA was induced by growth hormone (gibberellic acid) and stress hormones (abscisic acid, jasmonic acid, and salicylic acid). By using transgenic analysis, we showed that the upregulation of PagGASA in poplar provides high tolerance to drought stress. Therefore, our results suggest that PagGASA plays an important role in drought stress tolerance via stress-related plant hormone signaling in poplar.
Keywords
Drought stress; Gene expression; PagGASA; transgenic poplar;
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1 Alonso-Ramirez A, Rodriguez D, Reyes D, Jimenez JA, Nicolas G, Lopez-Climent M, Gomez-Cadenas A, Nicolas C (2009) Evidence for a role of gibberellins in salicylic acid-modulated early plant responses to abiotic stress in Arabidopsis seeds. Plant Physiol 150:1335-1344   DOI
2 Atkinson NJ, Urwin PE (2012) The interaction of plant biotic and abiotic stresses: from genes to the field. J Exp Bot 63: 3523-3543   DOI
3 Aubert D, Chevillard M, Dorne AM, Arlaud G, Herzog M (1998) Expression patterns of GASA genes in Arabidopsis thaliana: the GASA4 gene is upregulated by gibberellins in meristematic regions. Plant Mol Biol 36:871-883   DOI
4 Ballen-Taborda C, Plata G, Ayling S, Rodriguez-Zapata F, Lopez-Lavalle LAB, Duitama J, Tohme J (2013) Identification of cassava microRNAs under abiotic stress. Int J Genomics 2013:articleID 857986
5 Ben-Nissan G, Weiss D (1996) The petunia homologue of tomato gast1: transcript accumulation coincides with gibberellin-induced corolla cell elongation. Plant Mol Biol 32:1067-1074   DOI
6 Ben-Nissan G, Lee JY, Borohov A, Weiss D (2004) GIP, a Petunia hybrida GA-induced cysteine-rich protein: a possible role in shoot elongation and transition to flowering. Plant J 37:229-238   DOI
7 Bouquin T, Meier C, Foster R, Nielsen ME, Mundy J (2001) Control of specific gene expression by gibberellins and brassinosteroid. Plant Physiol 127:450-458   DOI
8 Cheng H, Qin L, Lee S, Fu X, Richards DE, Cao D, Luo D, Harberd NP, Peng J (2004) Gibberellin regulates Arabidopsis floral development via suppression of DELLA protein function. Development 131:1055-1064   DOI
9 de la Fuente JI, Amaya I, Castillejo C, Sanchez-Sevilla JF, Quesada MA, Botella MA, Valpuesta V (2006) The strawberry gene FaGAST affects plant growth through inhibition of cell elongation. J Exp Bot 57:2401-2411   DOI
10 Furukawa T, Sakaguchi N, Shimada H (2006) Two OsGASR genes, rice GAST homologue genes that are abundant in proliferating tissues, show different expression patterns in developing panicles. Genes Genet Syst 81:171-180   DOI
11 Golldack D, Luking I, Yang OS (2011) Plant tolerance to drought and salinity: stress regulating transcription factors and their functional significance in the cellular transcriptional network. Plant Cell Rep 30:1383-1391   DOI
12 Herzog M, Dorne AM, Grellet F (1995) GASA, a gibberellinregulated gene family from Arabidopsis thaliana related to the tomato GAST1 gene. Plant Mol Biol 27:743-752   DOI
13 Hirayama T, Shinozaki K (2010) Research on plant abiotic stress responses in the post-genome era: past, present and future. Plant J 61:1041-1052   DOI
14 Kim YH, Kim MD, Choi YI, Park SC, Yun DJ, Noh EW, Lee HS, Kwak SS (2011) Transgenic poplar expressing Arabidopsis NDPK2 enhances growth as well as oxidative stress tolerance. Plant Biotechnol J 9:334-347   DOI
15 Ko CB, Woo YM, Lee DJ, Lee MC, Kim CS (2007) Enhanced tolerance to heat stress in transgenic plants expressing the GASA4 gene. Plant Physiol Biochem 45:722-728   DOI
16 Kotilainen M, Helariutta Y, Mehto M, Pollanen E, Albert VA, Elomaa P, Teeri TH, (1999) GEG participates in the regulation of cell and organ shape during corolla and carpel development in Gerbera hybrida. Plant Cell 11:1093-1104   DOI
17 Lee HS, Lee JS, Noh EW, Bae EK, Choi YI, Han MS (2005) Generation and analysis of expressed sequence tags from poplar (Populus alba ${\times}$ P. tremula var. glandulosa) suspension cells. Plant Sci 169:1118-1124   DOI
18 Luo LJ (2010) Breeding for water-saving and drought-resistance rice (WDR) in China. J Exp Bot 61:3509-3517   DOI
19 Lee HS, Bae EK, Park SY, Sjodin A, Lee JS, Noh EW, Jansson S (2007) Growth-phase-dependent gene expression profiling of poplar (Populus alba ${\times}$ P. tremula var. glandulosa) suspension cells. Physiol Plantarum 131:599-613   DOI
20 Li KL, Bai X, Li Y, Cai H, Ji W, Tang LL, Wen YD, Zhu YM (2011) GsGASA1 mediated root growth inhibition in response to chronic cold stress is marked by the accumulation of DELLAs. J Plant Physiol 168:2153-2160   DOI
21 Nahirnak V, Almasia NI, Hopp HE, Vazquez-Rovere C (2012) Snakin/GASA proteins. Plant Signal Behav 7(8):1004-1008   DOI
22 Nakashima K, Yamaguchi-Shinozaki K, Shinozaki K (2014) The transcriptional regulatory network in the drought response and its crosstalk in abiotic stress responses including drought, cold, and heat. Frontiers in Plant Sci 5(170):25-31
23 Pfaffl MW (2001) A new mathematical model for relative quantification in real-time RT-PCR. Nucleic Acids Res 29(9):e45   DOI
24 Qu J, Kang SG, Hah C, Jang J-C (2016) Molecular and cellular characterization of GA-Stimultaed Transcripts GASA4 and GASA6 in Arabidopsis thaliana. Plant Sci 246:1-10   DOI
25 Roxrud J, Lid SE, Fletcher JC, Schmidt ED, Opsahl-Sorteberg HG (2007) GASA4, one of the 14-member Arabidopsis GASA family of small polypeptides, regulates flowering and seed development. Plant Cell Physiol 48:471-483   DOI
26 Zhang S, Wang X (2008) Expression pattern of GASA, downstream genes of DELLA, in Arabidopsis. Chinese Sci Bull 53: 3839-3846
27 Shi L, Gast RT, Gopalraj M, Olszewski NE (1992) Characterization of a shoot-specific, GA3-and ABA-regulated gene from tomato. Plant J 2:153-159
28 Sun S, Wang H, Yu H, Zhong C, Zhang X, Peng J, Wang X (2013) GASA14 regulates leaf expansion and abiotic stress resistance by modulating reactive oxygen species accumulation. J Exp Bot 64:1637-1647   DOI
29 Taylor BH, Scheuring CF (1994) A molecular marker for lateral root initiation: the RSI-1 gene of tomato (Lycopersicon esculentum Mill) is activated in early lateral root primordial. Mol Gen Genet 243:148-157
30 Wang L, Wang Z, Xu Y, Joo SH, Kim SK, Xue Z, Xu Z, Wang Z, Chong K (2009) OsGSR1 is involved in crosstalk between gibberellins and brassinosteroids in rice. Plant J 57:498-510   DOI
31 Zhang S, Yang C, Peng J, Sun S, Wang X (2009) GASA5, a regulator of flowering time and stem growth in Arabidopsis thaliana. Plant Mol Biol 69:745-759   DOI
32 Zhong C, Xu H, Ye S, Wang S, Li L, Zhang S, Wang X (2015) AtGASA6 serves as an integrator of gibberellin-, abscisic acidand glucose-signaling during seed germination in Arabidopsis. Plant Physiol 169:2288-2303
33 Zimmermann R, Sakai H, Hochholdinger F (2010) The gibberellic acid stimulated-like gene family in maize and its role in lateral root development. Plant Physiol 152:356-365   DOI