Journal of Embryo Transfer (한국수정란이식학회지)

The Korean Society of Embryo Transfer (한국수정란이식학회)
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Physiological Evaluation of Cloned Mini-pigs in a Transportable Isolator for the Study of Xenotransplantation

바이오장기 연구를 위한 이동식 아이솔레이터 내 복제 미니 돼지의 생리 활성 평가

  • Kim, Hae-Sung (College of Veterinary Medicine, Chungbuk National University) ;
  • Jeon, Yu-Byeol (College of Veterinary Medicine, Chungbuk National University) ;
  • Kwak, Seong-Sung (College of Veterinary Medicine, Chungbuk National University) ;
  • Jeong, Seung-A (College of Veterinary Medicine, Chungbuk National University) ;
  • Jung, Eui-Man (College of Veterinary Medicine, Chungbuk National University) ;
  • Hyun, Sang-Hwan (College of Veterinary Medicine, Chungbuk National University) ;
  • Jeung, Eui-Bae (College of Veterinary Medicine, Chungbuk National University)
  • Received : 2011.08.02
  • Accepted : 2011.08.19
  • Published : 2011.09.30
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Abstract

The present study investigated the physiological evaluation of cloned mini-pigs in a transportable isolator. Transportable isolator was designed and manufactured by our research team for transporting gnotobiotic pig. Until now, no previous reports are available regarding the physiological activities and harmful effects when pigs were transported in this isolator. Five cloned mini-pigs of 1~2 year (s) old female with a body weight between 80~90 kg were used. The effects of transportable isolator on stress-related hormone, adrenocorticotrophic hormone (ACTH) and cortisol levels, and heart rate were evaluated. In addition, it was also examined the effects of transportable isolator on blood chemistry factors (alanine aminotransferase: ALT, aspartate aminotransferase: AST, blood urea nitrogen: BUN, glucose, and creatinine). Blood was sampled just before the beginning of transport (T0), at the end of transport (30min after the transport; T1), and 30 min after the end of transport (T2). At the same time, heart rate was also evaluated. As a result, heart rate had no significant (p>0.05) differences at the various-time points of study (T0, T1, T2). However, heart rate was slightly higher than normal range in T1 and T2. The ACTH level was higher than normal range. Whereas, the cortisol level was lower than normal range. There were no statistical significant differences both ACTH and cortisol level between different time groups. Also, there were no significant differences in blood chemistry factors. Therefore, our present study shows that transportable isolator has no harmful effect on stress and physiological condition in cloned mini-pigs.

Keywords

References

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