• Title/Summary/Keyword: Strawberry

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Dipping Strawberry Plants in Fungicides before Planting to Control Anthracnose (딸기 탄저병 방제를 위한 정식 전 살균제 침지처리 효과)

  • Nam, Myeong Hyeon;Lee, In Ha;Kim, Hong Gi
    • Research in Plant Disease
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    • v.20 no.1
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    • pp.54-58
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    • 2014
  • Anthracnose crown rot (ACR), caused by Colletotrichum fructicola, is a serious disease of strawberry in Korea. The primary inoculums of ACR were symptomless strawberry plants, plant debris, and other host plants. To effectively control anthracnose in symptomless transplanted strawberries, it is necessary to use disease-free plants, detect the disease early, and apply a fungicide. Therefore, in 2010 and 2011, we evaluated the efficacy of pre-plant fungicide dips by using strawberry transplants infected by C. fructicola for the control of anthracnose. Dipping plants in prochloraz-Mn for 10 min before planting was most effective for controlling anthracnose in symptomless strawberry plants and resulted in more than 76% control efficacy. Azoxystrobin showed a control efficacy of over 40%, but plants treated with pyraclostrobin, mancozeb and iminoctadine tris showed high disease severity. The control efficacy of the dip treatment with prochloraz-Mn did not differ with temperature and time. Treatment with prochloraz-Mn for more than an hour caused growth suppression in strawberry plants. Therefore, the development of anthracnose can be effectively reduced by dipping strawberry plants for 10 min in prochloraz-Mn before planting.

Development of transgenic strawberry plants expressing monellin, a sweet protein (감미단백질 모넬린 발현 딸기 형질전환 식물체 개발)

  • Min, Sung Ran;Ko, Suk Min;Lyu, Jae Il;Park, Ji Hyun;Yi, So Young;Lee, In-Ha;Kim, Hyun Sook;Kim, Tae Il;Choi, Pil Son;Jeong, Won-Joong;Kim, Suk Weon;Kim, Jonghyun;Liu, Jang R.
    • Journal of Plant Biotechnology
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    • v.42 no.3
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    • pp.180-185
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    • 2015
  • Leaf discs from 'Yeobong' and 'Maehyang' strawberry plants were used as explants for transformation. The Agrobacterium tumefaciens strain EHA105 harboring the monellin gene under the control of the CaMV 35S promoter was used in co-cultivation experiments. The frequencies of callus formation and plant regeneration from leaf explants after co-cultivation in 'Yeobong' were higher than those of 'Maehyang'. These transgenic plants showed normal growth patterns and flowering. PCR and Southern hybridization confirmed that 1 to 2 copies of the monellin gene were integrated into genome of the transgenic strawberry plants. Northern blot analysis confirm that the transcripts were expressed in transgenic strawberry plants. Although long-term subcultured transgenic strawberry plants showed a phenomenon to escape the transgene, the transformation system established in this study provides new opportunities for genetic improvement of strawberry plants.

Effects of Supplemental LEDs on the Fruit Quality of Two Strawberry (Fragaria × ananassa Duch.) Cultivars due to Ripening Level (LED 보광이 딸기 두 품종의 성숙도에 따른 과실 품질에 미치는 영향)

  • Choi, Hyo Gil;Jeong, Ho Jeong
    • Journal of Bio-Environment Control
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    • v.28 no.4
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    • pp.302-310
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    • 2019
  • This study was conducted to investigate the effects of LEDs on the fruit qualities of two strawberry (Fragaria ${\times}$ ananassa Duch.) cultivars such as hardness, phytochemicals, and antioxidant activity using the strawberry fruits, which were harvested by 50% and 100% ripening levels of fruits grown under LEDs illuminated during 6 hours after sunset from November 2018 to January of the following year. In the hardness of strawberry fruit, when two strawberry cultivars were illuminated red LED light, in 50% ripening as well as 100% ripening fruit of both 'Daewang' and 'Seolhyang' cultivars were significantly higher compared to other treatments. Also, in the sugar content on 50% ripening fruit of two cultivars, the fruit of red LED light was significantly higher than in the other LEDs and control. On the other hand, in the acidity of 50% and 100% ripening levels of strawberry fruit, the fruit under control condition was higher in than that of LEDs. The phenolic compounds of strawberry fruit grown in control was much higher than that of strawberry treated with LEDs. However, the influence of LEDs on flavonoid and anthocyanin content of strawberry fruit did not affected. Changes in the phytochemicals contents of the strawberry tends to be affected depending on the maturity of fruit. Antioxidant activity such as DPPH and ABTS of were not different by maturity of fruit, and supplementation of LEDs during 6 hours at night. Therefore, we concluded that lighting of LEDs is effective for fruit quality in terms of sugar accumulation and fruit hardness.

Development and shelf-life determination of senior-friendly strawberry jelly (고령자 친화 딸기 젤리 개발 및 저장수명 결정)

  • Kim, Ji Hyeon;Min, Sea Cheol
    • Korean Journal of Food Science and Technology
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    • v.49 no.2
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    • pp.181-185
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    • 2017
  • Senior-friendly strawberry jelly was developed using strawberry juice, sugar, xanthan gum, and locust bean gum. The experimental variables included strawberry juice (30.0-40.0%, w/w) and sugar (7.5-10.0%, w/w) concentrations and the xanthan gum/locust bean gum ratio (0.3-4.0), and interactions among these variables were predicted using a response surface methodology. The optimal concentrations of strawberry juice and sugar and the ratio of xanthan gum/locust bean gum, determined against jelly hardness, were found to be 40.0, 10.0, and 1.5%, respectively. The hardness, gumminess, and chewiness of strawberry jelly increased significantly during storage at 5 and $15^{\circ}C$ (p<0.05). The lightness of the gum decreased significantly at both temperatures (p<0.05), whereas the hue angle increased during storage at $15^{\circ}C$. The zero-order kinetics was used to predict the shelf-life of the prepared jelly ($R^2=0.89-0.96$), which was determined to be 38 and 26 days at 5 and $15^{\circ}C$, respectively.

Investigation of Genetic Diversity of Fusarium oxysporum f. sp. fragariae Using PCR-RFLP

  • Kim, Ji-Su;Kang, Nam Jun;Kwak, Youn-Sig;Lee, Choungkeun
    • The Plant Pathology Journal
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    • v.33 no.2
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    • pp.140-147
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    • 2017
  • Fusarium wilts of strawberry, caused by Fusarium oxysporum f. sp. fragariae, is a serious soil-borne disease. Fusarium wilt causes dramatic yield losses in commercial strawberry production and it is a very stubborn disease to control. Reliable chemical control of strawberry Fusarium wilt disease is not yet available. Moreover, other well-known F. oxysporum have different genetic information from F. oxysporum f. sp. fragariae. This analysis investigates the genetic diversity of strawberry Fusairum wilt pathogen. In total, 110 pathogens were isolated from three major strawberry production regions, namely Sukok, Hadong, Sancheong in Gyeongnam province in South Korea. The isolates were confirmed using F. oxysporum f. sp. fragariae species-specific primer sets. Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) analyses were executed using the internal transcribed spacer, intergenic spacer, translation elongation factor1-${\alpha}$, and ${\beta}$-tubulin genes of the pathogens and four restriction enzymes: AluI, HhaI, HinP1I and HpyCH4V. Regarding results, there were diverse patterns in the three gene regions except for the ${\beta}$-tubulin gene region. Correlation analysis of strawberry cultivation region, cultivation method, variety, and phenotype of isolated pathogen, confirmed that genetic diversity depended on the classification of the cultivated region.

Effect of Pectinase Treatment on Extraction Yield of the Juice of Fragaria ananassa Duch. and the Quality Characteristics of Strawberry Wine during Ethanolic Fermentation (딸기 과즙의 수율과 딸기 발효주의 품질에 대한 pectinase 처리의 영향)

  • Jeong, Eun-Jeong;Kim, Min-Hwa;Kim, Yong-Suk
    • Food Science and Preservation
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    • v.17 no.1
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    • pp.72-78
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    • 2010
  • To develop a low-ethanol strawberry wine, the use of pectinase to improve the extraction yield of strawberry juice was investigated, and changes in physicochemical characteristics during ethanolic fermentation were assessed. The juice yield from strawberry fruit increased by 18.9% after Viscozyme L treatment (1,000 ppm, 30 min), compared with a control group, a greater increase than seen with other pectinases (17.5-18.7%). No significant quality differences were observed between control juice and juice prepared with enzyme treatment, indicating that neither physicochemical characteristics nor ethanol content during fermentation were affected by pectinase treatment. The major pigments of strawberry juice were cyanidin-3-glucoside and pelargonidin-3-glucoside, both of which are anthocyanins. The pigment level after enzyme treatment was slightly lower than that of the control group, at all fermentation times. We consider that the economics of strawberry wine manufacture may be increased by use of pectinase because juice level was increased, but no change in ethanol content or physicochemical characteristics was apparent.

Efficacy by Application Schedule of Fungicides before Harvest Stage for Control of Strawberry Powdery Mildew (딸기 흰가루병 방제를 위한 수확전 약제 처리 시기)

  • Nam, Myeong-Hyeon;Jung, Suck-Kee;Jang, Chang-Soon;Song, Jeong-Young;Kim, Hong-Gi
    • Research in Plant Disease
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    • v.11 no.1
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    • pp.39-42
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    • 2005
  • Powdery mildew, caused by Sphaerotheca aphanis var. aphanis, is an economically significant disease of strawberry in Korea. When powdery mildew is not controlled adequately, it often spreads rapidly through strawberry plants and damage is associated with reduced yields. Proper timing of fungicide applications is, therefore, essential for effective disease control. This study evaluated the efficacy by application schedule of fungicides before harvest stage for preventing powdery mildew in 2001-2003. The systemic fungicides, azoxystrobin, kresoxim-methyl, and cupper fungicide DBEDC were applied preventively during the first part of the cultivating season. Preventative applications of DBEDC by dipping treatment before transplanting and kresoxim-methyl by foliar spray before blooming stage were one of the most effective control schedule tested to prevent and manage this disease. This research demonstrated the significance of application time to control of powdery mildew, particularly provided elimination unnecessary sprays of agrochemicals and reduction costs for strawberry growers.

Effect of Storage Condition on the Quality and Microbiological Change of Strawberry “Minyubong” during Storage (저장조건이 딸기 “미녀봉”의 저장 중 품질 및 미생물학적 변화에 미치는 영향)

  • Lee, Se-Hee;Lee, Myung-Suk;Namkyu Sun;Song, Kyung-Bin
    • Food Science and Preservation
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    • v.11 no.1
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    • pp.7-11
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    • 2004
  • To examine the effect of storage condition on the quality and microbiological change of strawberry “Minyubong”, the rate of weight loss, decay rate, pH and titratable acidity, and microbial (total bacterial count, mold and yeast) changes were determined during storage. Strawberry fruits were packaged with low-density polyethylene (LDPE). Strawberries were then stored at 4$^{\circ}C$ and 20$^{\circ}C$, respectively. LDPE package was effective on the decrease of decay rate of strawberry as well as the rate of weight loss, compared with the non-packaged. Microbial changes of strawberry stored at 4$^{\circ}C$ and 20$^{\circ}C$ were monitored during storage. Packaging affected the microbial change, resulting in retarding the growth of total bacteria as well as mold and yeast, compared with the non-packaged, regardless of storage temperature. These results indicate that storage of strawberry fruits wrapped with LDPE at 4'C should be recommended in terms of quality as well as its shelf-life.

Effects of Supplemental LED Lighting on Productivity and Fruit Quality of Strawberry (Fragaria × ananassa Duch.) Grown on the Bottom Bed of the Two-Bed Bench System (2단 베드 시스템의 하단부에서 자란 딸기의 생산성 및 과일 품질에 미치는 보광 LED의 효과)

  • Choi, Hyo Gil;Jeong, Ho Jeong;Choi, Gyeong Lee;Choi, Su Hyun;Chae, Soo Cheon;Ann, Seoung Won;Kang, Hee Kyoung;Kang, Nam Jun
    • Journal of Bio-Environment Control
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    • v.27 no.3
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    • pp.199-205
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    • 2018
  • The aim of this study was to confirm that effects of supplemental LED illumination on a strawberry yield and fruit quality when strawberry grown on a bottom bed to be deficient ambient light due to shading of a upper bed during cultivation by a two-bed bench system. A strawberry was cultivated as a drip irrigation system in the two-bed bench system filled with a strawberry exclusive media from October 2015 to January 2016. The upper and the bottom bed without LED illumination for growth of a strawberry were using as a control. For LED light treatments, from 10 am to 4 pm, we illuminated LEDs as $100{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$ of light intensity by using blue, red, and mixing LED (blue plus red) on the strawberry plants of the bottom bed. In the yield of strawberry fruit, the strawberry grown on the bottom bed treated with the blue LED significantly increased compared with that of the bottom bed part control, and increased to by near 90% of the strawberry output of the upper bed part control. The soluble sugar content of strawberry fruit grown on the upper bed part control and on the bottom bed illuminated with blue or mixed LED was higher than that of red LED and the control of the bottom bed. The content of anthocyanin was the highest increased in the strawberry grown on the upper bed part control that received a lot of ambient light, however when comparing only the bottom bed, strawberry fruits grown on all LED treatments were higher than that of the control. Therefore, we considered that using of the blue LED light on the bottom bed of two-bed bench system during strawberry cultivation is advantageous for the increase of yield and improvement of fruit quality.

Effect of Nutrition Solution pH and Electrical Conductivity on Fusarium Wilt on Strawberry Plants in Hydroponic Culture (딸기 수경재배에서 시들음병 발생에 배양액 pH와 EC 효과)

  • Nam, Myeong Hyeon;Lee, Hee Chul;Kim, Tae il;Lee, Eun Mo;Yoon, Hae Suk
    • Research in Plant Disease
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    • v.24 no.1
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    • pp.26-32
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    • 2018
  • Fusarium wilt on strawberry plants caused by Fusarium oxysporum f. sp. fragariae (Fof) is a major disease in Korea. The prevalence of this disease is increasing, especially in hydroponic cultivation in strawberry field. This study assessed the effect of nutrition solution pH and electrical conductivity (EC) on Fusarium wilt in vitro and in field trials. pH levels of 5.0, 5.5, 6.0, 6.5, 7.0, and 7.5 were assayed in vitro and in field trials. EC levels at 0, 0.5, 0.8, 1.0, and $1.5dS{\cdot}m^{-1}$ were assayed in field trials. Mycelial growth of Fof increased with increasing pH and was highest at $25^{\circ}C$ pH 7 and lowest at $20^{\circ}C$, pH 5.0 in vitro. The incidence of Fusarium wilt was lowest in the pH 6.5 treatment and highest in the pH 5 treatment in field trials. At higher pH levels, the EC decreased in the drain solution and the potassium content of strawberry leaves increased. In the EC assay, the severity of Fusarium wilt and nitrogen content of leaves increased as the EC increased. These results indicate that Fusarium wilt is related to pH and EC in hydroponic culture of strawberry plants.